Membre

@article{courtois:hal-03499742,

title = {Transfer of sulfidized silver from silver nanoparticles, in sewage sludge, to plants and primary consumers in agricultural soil environment},

author = {Pauline Courtois and Annette Vaufleury and Anna Grosser and Christine Lors and Franck Vandenbulcke},

url = {https://hal-univ-fcomte.archives-ouvertes.fr/hal-03499742},

doi = {10.1016/j.scitotenv.2021.145900},

year  = {2021},

date = {2021-07-01},

urldate = {2021-07-01},

journal = {Science of the Total Environment},

volume = {777},

pages = {145900},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{jaskulak:hal-03493603,

title = {Species-specific Cd-detoxification mechanisms in lumbricid earthworms Eisenia andrei, Eisenia fetida and their hybrids},

author = {Marta Jaskulak and Agnieszka Rorat and Ligia Kurianska-Piatek and Sebastian Hofman and Janusz Bigaj and Franck Vandenbulcke and Barbara Plytycz},

url = {https://hal.archives-ouvertes.fr/hal-03493603},

doi = {10.1016/j.ecoenv.2020.111425},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {208},

pages = {111425 -},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{courtois:hal-03149939,

title = {Medium-term effects of Ag supplied directly or via sewage sludge to an agricultural soil on Eisenia fetida earthworm and soil microbial communities},

author = {Pauline Courtois and Agnieszka Rorat and Sébastien Lemiere and Remy Guyoneaud and Eléonore Attard and Manon Longepierre and Franc cois Rigal and Clément Levard and Perrine Chaurand and Anna Grosser and Anna Grobelak and Malgorzata Kacprzak and Christine Lors and Agnes Richaume and Franck Vandenbulcke},

url = {https://hal.archives-ouvertes.fr/hal-03149939},

doi = {10.1016/j.chemosphere.2020.128761},

year  = {2021},

date = {2021-04-01},

urldate = {2021-04-01},

journal = {Chemosphere},

volume = {269},

pages = {128761},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{grosser:hal-03175993,

title = {Effects of silver nanoparticles on performance of anaerobic digestion of sewage sludge and associated microbial communities},

author = {Anna Grosser and Anna Grobelak and Agnieszka Rorat and Pauline Courtois and Franck Vandenbulcke and Sébastien Lemi`ere and Remy Guyoneaud and Eléonore Attard and Piotr Celary},

url = {https://hal.archives-ouvertes.fr/hal-03175993},

doi = {10.1016/j.renene.2021.02.127},

year  = {2021},

date = {2021-06-01},

urldate = {2021-06-01},

journal = {Renewable Energy},

volume = {171},

pages = {1014-1025},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Audusseau2020,

title = {Impacts of metallic trace elements on an earthworm community in an urban wasteland: Emphasis on the bioaccumulation and genetic characteristics in Lumbricus castaneus},

author = {Hélène Audusseau and Franck Vandenbulcke and Cassandre Dume and Valentin Deschins and Maxime Pauwels and Agnès Gigon and Matthieu Bagard and Lise Dupont},

year  = {2020},

date = {2020-01-01},

journal = {Science of the Total Environment},

volume = {718},

pages = {9},

abstract = {Metallic trace elements (MTEs) soil pollution has become a worldwide 

	concern, particularly regarding its impact on earthworms. Earthworms, 

	which constitute the dominant taxon of soil macrofauna in temperate 

	regions and are crucial ecosystem engineers, are in direct contact 

	with MTEs. The impacts of MTE exposure on earthworms, however, vary 

	by species, with some able to cope with high levels of contamination. 

	We combined different approaches to study the effects of MTEs at 

	different levels of biological organisation of an earthworm community, 

	in a contaminated urban wasteland. Our work is based on field collection 

	of soil and earthworm samples, with a total of 891 adult earthworms 

	from 8 species collected, over 87 quadrats across the study plot. 

	We found that MTE concentrations are highly structured at the plot 

	scale and that some elements, such as Pb, Zn, and Cu. are highly 

	correlated. Comparing species assemblage to MTE concentrations, we 

	found that the juvenile and adult abundances, and community composition, 

	were significantly affected by pollution. Along the pollution gradient, 

	as species richness decreased. Lumbricus castaneus became more dominant. 

	We thus investigated the physiological response of this species to 

	a set of specific elements (Pb, Zn, Cu, and Cd) and studied the impacts 

	of MTE concentrations at the plot scale on its population genetic. 

	These analyses revealed that L castaneus is able to bioaccumulate 

	high quantities of Cd and Zn, but not of Cu and Pb. The population 

	genetic analysis, based on the genotyping of 175 individuals using 

	8 microsatellite markers, provided no evidence of the role of the 

	heterogeneity in MTE concentrations as a barrier to gene flow. The 

	multidisciplinary approach we used enabled us to reveal the comparatively 

	high tolerance of L castaneus to MTE concentrations, suggesting that 

	this is a promising model to study the molecular bases of MTE tolerance. 

	(C) 2020 Elsevier B.V. All rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Courtois2020,

title = {Accumulation, speciation and localization of silver nanoparticles in the earthworm Eisenia fetida},

author = {Pauline Courtois and Agnieszka Rorat and Sébastien Lemiere and Clément Levard and Perrine Chaurand and Anna Grobelak and Christine Lors and Franck Vandenbulcke},

year  = {2020},

date = {2020-01-01},

journal = {Environmental Science and Pollution Research},

pages = {10},

abstract = {The use of silver nanoparticles (AgNPs) in agriculture and many consumer 

	products has led to a significant release of Ag in the environment. 

	Although Ag toxicity in terrestrial organisms has been studied extensively, 

	very little is known about the accumulation capacity and coping mechanisms 

	of organisms in Ag-contaminated soil. In this context, we exposed 

	Eisenia fetida earthworms to artificial OECD soil spiked with a range 

	of concentrations of Ag (AgNPs or AgNO3). The main aims were to (1) 

	identify the location and form of accumulation of Ag in the exposed 

	earthworms and (2) better understand the physiological mechanisms 

	involved in Ag detoxification. The results showed that similar doses 

	of AgNPs or AgNO3 did not have the same effect on E. fetida survival. 

	The two forms of Ag added to soil exhibited substantial differences 

	in speciation at the end of exposure, but the Ag speciation and content 

	of Ag in earthworms were similar, suggesting that biotransformation 

	of Ag occurred. Finally, 3D images of intact earthworms obtained 

	by X-ray micro-computed tomography revealed that Ag accumulated preferentially 

	in the chloragogen tissue, coelomocytes, and nephridial epithelium. 

	Thus, E. fetida bioaccumulates Ag, but a regulation mechanism limits 

	its impact in a very efficient manner. The location of Ag in the 

	organism, the competition between Ag and Cu, and the speciation of 

	internal Ag suggest a link between Ag and the thiol-rich proteins 

	that are widely present in these tissues, most probably metallothioneins, 

	which are key proteins in the sequestration and detoxification of 

	metals.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Jaskulak2020,

title = {Modelling assisted phytoremediation of soils contaminated with heavy metals - Main opportunities, limitations, decision making and future prospects},

author = {Marta Jaskulak and Anna Grobelak and Franck Vandenbulcke},

year  = {2020},

date = {2020-01-01},

journal = {Chemosphere},

volume = {249},

pages = {126196},

abstract = {The heavy metals (HMs) soils contamination is a growing concern since 

	HMs are not biodegradable and can accumulate in all living organisms 

	causing a threat to plants and animals, including humans. Phytoremediation 

	is a cost-efficient technology that uses plants to remove, transform 

	or detoxify contaminants. In recent years, phytoremediation is entering 

	the stage of large-scale modelling via various mathematical models. 

	Such models can be useful tools to further our understanding and 

	predicting of the processes that influence the efficiency of phytoremediation 

	and to precisely plan such actions on a large-scale. When dealing 

	with extremely complicated and challenging variables like the interactions 

	between the climate, soil and plants, modelling before starting an 

	operation can significantly reduce the time and cost of such process 

	by granting us an accurate prediction of possible outcomes. Research 

	on the applicability of different modelling approaches is ongoing 

	and presented work compares and discusses available models in order 

	to point out their specific strengths and weaknesses in given scenarios. 

	The main aim of this paper is to critically evaluate the main advantages 

	and limitations of available models for large-scale phytoremediation 

	including, among others, the Decision Support System (DSS), Response 

	Surface Methodology (RSM), BALANS, PLANTIX and various regression 

	models. Study compares their applicability and highlight existing 

	gaps in current knowledge with a special reference to improving the 

	efficiency of large-scale phytoremediation of sites contaminated 

	with heavy-metals. The presented work can serve as a useful tool 

	when choosing the most suitable model for the phytoremediation of 

	contaminated sites. (C) 2020 Elsevier Ltd. All rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Jaskulak2020a,

title = {Effects of sewage sludge supplementation on heavy metal accumulation and the expression of ABC transporters in Sinapis alba L. during assisted phytoremediation of contaminated sites},

author = {Marta Jaskulak and Anna Grobelak and Franck Vandenbulcke},

year  = {2020},

date = {2020-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {197},

pages = {12},

abstract = {ATP binding cassette (ABC) transporters, types C, G, and B were monitored 

	via qPCR in order to investigate the influence of heavy metal (HM) 

	contamination of post-industrial and post-agricultural soils and 

	the effects of its supplementation with sewage sludge, on Sinapis 

	alba plants. Five house-keeping genes were selected and validated 

	to ensure the best reference points. The relative expression of ABC 

	types C and G genes was profoundly affected by experimental conditions 

	and included their upregulation after plants exposure to heavy metals 

	and downregulation after supplementation with sewage sludge. However, 

	ABC type C was more responsive then type G. The experimental conditions 

	altered the expression of ABC type C gene faster than ABC type G 

	and thus, the expression of ABC type C can therefore potentially 

	be used as a bioindicator during assisted phytoremediation of degraded 

	sites. In clean soil, supplementation with sewage sludge with a slight 

	content of heavy metals still caused an upregulation in the expression 

	of ABC types C and G, which showed that proper toxicity assessments 

	are necessary to ensure safe application of sewage sludge into soils. 

	Results showed that the analysed genes take a significant part in 

	plants metal detoxification and that their expression is regulated 

	at transcriptional level after exposure to soil contaminated with 

	heavy metals by both, industrial activities and by sewage sludge 

	supplementation. Thus, their expression can potentially be used as 

	an early-warning biomarker when soil supplementation with sewage 

	sludge is incorporated into the soil-management process.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Jaskulak2020c,

title = {Modeling and optimizing the removal of cadmium by Sinapis alba L. from contaminated soil via Response Surface Methodology and Artificial Neural Networks during assisted phytoremediation with sewage sludge},

author = {Marta Jaskulak and Anna Grobelak and Franck Vandenbulcke},

year  = {2020},

date = {2020-01-01},

journal = {International Journal of Phytoremediation},

volume = {22},

number = {12},

pages = {1321-1330},

abstract = {The study was aimed to model and optimize the removal of cadmium from 

	contaminated post-industrial soil viaSinapis albaL. by comparing 

	two modeling approaches: Response Surface Methodology (RSM) and Artificial 

	Neural Networks (ANN). The experimental design was done using the 

	Box-Behnken Design method. In the RSM model, the quadratic model 

	was shown to predict the closest results in comparison to our experimental 

	data. For ANN approach, a two-layer Feed-Forward Back-Propagation 

	Neural Network model was designed. The results showed that sewage 

	sludge supplementation increased the efficiency of theSinapis albaplant 

	in removing Cd from the soil. After 28 days of exposure, the removal 

	rate varied from 10.96% without any supplementation to 65.9% after 

	supplementation with the highest possible (law allowed) dose of sewage 

	sludge. The comparison proved that the prediction capability of the 

	ANN model was much higher than that of the RSM model (adjustedR-square: 

	0.98, standard error of the Cd prediction removal: 0.85 +/- 0.02). 

	Thus, the ANN model could be used for the prediction of heavy metal 

	removal during assisted phytoremediation with sewage sludge. Moreover, 

	such approach could also be used to determinate the dose of sewage 

	sludge that will ensure highest process efficiency.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Lanier2019,

title = {Combined toxic effects and DNA damage to two plant species exposed to binary metal mixtures (Cd/Pb)},

author = {Caroline Lanier and Fabien Bernard and Sylvain Dumez and Julie Leclercq-Dransart and Sébastien Lemière and Franck Vandenbulcke and Fabrice Nesslany and Anne Platel and Inès Devred and Audrey Hayet and Damien Cuny and Annabelle Deram},

year  = {2019},

date = {2019-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {167},

pages = {278-287},

abstract = {Acute and long-term (3-, 10- and 56-day exposure) laboratory toxicity 

	tests were carried out to assess the individual and combined toxic 

	effects of cadmium (Cd) and lead (Pb) in Brassica oleracea and Trifolium 

	repens. In addition to morphological parameters, this work also used 

	comet assay to address endpoints in relation to genotoxicity. Bioaccumulation 

	was measured to demonstrate the influence of the mixture on the concentrations 

	of each metal in the plant. 

 

	The statistical method reported by Ince et al. (1999) was used to 

	evaluate the types of interaction between Cd and Pb in each treatment 

	and concerning their combined effect. 

 

	This study concludes that the combined effects of binary metal combinations 

	of Cd/Pb on morphological parameters are most often additive, sometimes 

	antagonistic and more rarely synergistic, thus extending the findings 

	of previous publications on this subject. 

 

	DNA damage analysis revealed concentration- and time-dependent interactions. 

	Synergistic effects of mixed metals (more breaks than individually 

	applied metals) are observed in T. repens after a short exposure. 

	Antagonistic effects are statistically significant after 10 days-exposure, 

	suggesting competition between metals. At 56 days, the rate of DNA 

	damage observed in plants exposed to the Cd/Pb mixture was similar 

	to that measured in plants exposed to lead only and was significantly 

	lower than the rate of DNA damage induced by Cd. This supports the 

	idea that there may be competition between metals and also strengthens 

	the hypothesis that long-term reparation mechanisms may be implemented. 

	Cd/Pb co-exposure does not significantly influence the bioaccumulation 

	of each metal. It is nevertheless important to note that a statistically 

	significant ‘interaction’ is not necessarily biologically relevant 

	and should therefore be considered with caution when assessing heavy 

	metals combined effects},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Courtois2019,

title = {Ecotoxicology of silver nanoparticles and their derivatives introduced in soil with or without sewage sludge: A review of effects on microorganisms, plants and animals},

author = {Pauline Courtois and Agnieszka Rorat and Sébastien Lemiere and Remy Guyoneaud and Eleonore Attard and Clément Levard and Franck Vandenbulcke},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Pollution},

volume = {253},

pages = {578-598},

abstract = {Silver nanoparticles (AgNPs) are widely incorporated in many products, 

	partly due to their antimicrobial properties. The subsequent discharge 

	of this form of silver into wastewater leads to an accumulation of 

	silver species (AgNPs and derivatives resulting from their chemical 

	transformation), in sewage sludge. As a result of the land application 

	of sewage sludge for agricultural or remediation purposes, soils 

	are the primary receiver media of silver contamination. Research 

	on the long-term impact of AgNPs on the environment is ongoing, and 

	this paper is the first review that summarizes the existing state 

	of scientific knowledge on the potential impact of silver species 

	introduced into the soil via sewage sludge, from microorganisms to 

	earthworms and plants. Silver species can easily enter cells through 

	biological membranes and affect the physiology of organisms, resulting 

	in toxic effects. In soils, exposure to AgNPs may change microbial 

	biomass and diversity, decrease plant growth and inhibit soil invertebrate 

	reproduction. Physiological, biochemical and molecular effects have 

	been documented in various soil organisms and microorganisms. Negative 

	effects on organisms of the dominant form of silver in sewage sludge, 

	silver sulfide (Ag2S), have been observed, although these effects 

	are attenuated compared to the effects of metallic AgNPs. However, 

	silver toxicity is complex to evaluate and much remains unknown about 

	the ecotoxicology of silver species in soils, especially with respect 

	to the possibility of transfer along the trophic chain via accumulation 

	in plant and animal tissues. Critical points related to the hazards 

	associated with the presence of silver species in the environment 

	are described, and important issues concerning the ecotoxicity of 

	sewage sludge applied to soil are discussed to highlight gaps in 

	existing scientific knowledge and essential research directions for 

	improving risk assessment. (C) 2019 Elsevier Ltd. All rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Dupont2019,

title = {Genetic variation of the epigeic earthworm Lumbricus castaneus populations in urban soils of the Paris region (France) revealed using eight newly developed microsatellite markers},

author = {Lise Dupont and Maxime Pauwels and Cassandre Dume and Valentin Deschins and Hélène Audusseau and Agnès Gigon and Florence Dubs and Franck Vandenbulcke},

year  = {2019},

date = {2019-01-01},

journal = {Applied Soil Ecology},

volume = {135},

pages = {33-37},

abstract = {Urban soils are subject to intense environmental pressures, e.g. physical 

	disturbance, surface transformation and pollution, which greatly 

	impact the activity of soil organisms. The epigeic earthworm Lumbricus 

	castaneus (Savigny, 1826) is known to be tolerant to trace elements 

	contamination of soils and was found to be abundant in the urban 

	context. In order to investigate how urban environmental stressors 

	shape the population genetic variation of this species, we developed 

	8 microsatellite loci from a microsatellite-enriched genomic library. 

	Polymorphism was explored in 6 populations (153 individuals) from 

	the Paris region. The number of alleles per locus varied from 7 to 

	29. The average expected heterozygosity within populations ranged 

	from 0.596 to 0.705. These new microsatellite markers revealed a 

	significant genetic structure at fine spatial scale, highlighting 

	that they could be particularly useful for genetic studies of populations 

	in urban soils.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Jaskulak2019,

title = {Gene expression, DNA damage and other stress markers in Sinapis alba L. exposed to heavy metals with special reference to sewage sludge application on contaminated sites},

author = {Marta Jaskulak and Anna Grobelak and Anna Grosser and Franck Vandenbulcke},

year  = {2019},

date = {2019-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {181},

pages = {508-517},

abstract = {Bioindicators are promising tools used to detect the long-term effects 

	of selected biosolids on plants development and should be implemented 

	before large-scale supplementation of sewage sludge into the soil. 

	The presented study shows the impact of sewage sludge application 

	on metal-sensitive toxicity biological parameters (biomarkers) in 

	Sinapis alba including: germination, root length, the activity of 

	guaiacol peroxidase, the chlorophyll content, the level of DNA damage 

	and the expression level of Ribulose-1,5-bisphosphate carboxylase/oxygenase 

	(rbcL) and metallothionein (mt). We evaluated data from selected 

	biomarkers in order to broaden our understanding of plants defense 

	mechanisms against heavy metal contamination and the application 

	of sewage sludge into soils. Overall, in contaminated soil after 

	supplementation with both municipal sewage sludges, an increase in 

	toxicity was noticed in DNA damage, mt and rbcl expression and total 

	chlorophyll content. The supplementation of both soils with municipal 

	sewage sludge caused a two-time induction in the mt expression. Moreover, 

	clean soil supplemented with sewage sludge caused an increase in 

	DNA damage shown as the tail moment from approximately 12 mu m on 

	control to 40 mu m after supplementation. Even if those biosolids 

	increased the initial germination, roots length, and biomass in comparison 

	to the unamended soil, the toxicity was evidenced with other stress 

	markers. Results showed, that in order to accurately assess the influence 

	of sewage sludge application on plants the use of several specific 

	biomarkers is required for safe land restoration. The conducted study 

	also confirmed, both under biochemical and genotoxic tests, that 

	iron enrichment for biosolids or contaminated soil can significantly 

	reduce the bioavailability and toxicity of other metals.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Jaskulak2019a,

title = {Bioaccumulation, antioxidative response, and metallothionein expression in Lupinus luteus L. exposed to heavy metals and silver nanoparticles},

author = {Marta Jaskulak and Agnieszka Rorat and Anna Grobelak and Zayneb Chaabene and Malgorzata Kacprzak and Franck Vandenbulcke},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Science and Pollution Research},

volume = {26},

number = {16},

pages = {16040-16052},

abstract = {Yellow-lupin (Lupinus luteus L.) was grown on soils contaminated with 

	heavy metals during two parallel studies. In the first one, the soil 

	was contaminated by industrial activities whereas, in the second 

	one, the soil was artificially contaminated with a single metal including 

	Cd, Pb, Zn, Ni (in nitrate form), and Ag (in nitrate and nanoparticles 

	form). The study was performed to assess a plant's response to contamination 

	including its antioxidative response and molecular mechanisms involved 

	in metal detoxification through the expression level of metallothioneins 

	(MTs). Overall, the study provided insights into identification and 

	validation of housekeeping genes (HKG) in L. luteus under exposure 

	to metal stress and showed the effects of selected heavy metals and 

	silver nanoparticles on the expression of metallothioneins, the activity 

	of guaiacol peroxidase (GPX) and bioaccumulation of metals in leaves 

	of L. luteus. As such, HKG validation using BestKeeper, NormFinder, 

	and geNorm software allowed for the selection of four most stable 

	reference genes in a context metal contamination for the selected 

	plant. Moreover, a significant increase in the expression levels 

	of MT was observed in plants grown under heavy metal stress and none 

	on plants grown on 25mgkg(-1) of silver nanoparticles. Also, the 

	GPX activity and MT expression showed statistically significant changes 

	between different conditions and doses which means that they can 

	be used as highly sensitive stress markers for planning the phytoremediation 

	process on a large scale.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Chaabene2018,

title = {Copper toxicity and date palm (Phoenix dactylifera) seedling tolerance: Monitoring of related biomarker},

author = {Zayneb Chaâbene and Imen Rekik-Hakim and Agnieszka Rorat and Amine Elleuch and Hafedh Mejdoub and Franck Vandenbulcke},

year  = {2018},

date = {2018-01-01},

journal = {Environmental Toxicology and Chemistry},

volume = {Article in Press. DOI: 10.1002/etc.4007},

abstract = {Date palm (Phoenix dactylifera) seeds were exposed to different copper (Cu) solutions to examine plant stress responses. Low Cu concentrations (0.02 and 0.2 mM) caused an increase of seed germination, whereas higher Cu amounts (2 mM) significantly inhibited seed germination, delayed hypocotyl elongation, increased seedling mortality, and reduced the germination index by more than 90%. Metal-related toxicity symptoms appeared after 15 d of 2 mM of Cu exposure. Biochemical activities such as amylase activity and redox balance elements were examined to study the relationship between external Cu amount and internal plant response. The present study showed that amylolytic activity	was dose- and time-dependent. Likewise, H2 O2 production increased after exposure to Cu, which was correlated with thiobarbituric acid reactive substance (TBARS) accumulation. Furthermore at low Cu concentrations, superoxide dismutase (SOD) and catalase (CAT) activities increased, suggesting that date palm seed stimulated its metal homeostasis networks.	However, the highest cupric ion amounts increased cell oxidant accumulation and reduced enzyme production. Gene expression level measures of P. dactylifera phytochelatin synthase (Pdpcs) and P. dactylifera metallothionein (Pdmt) encoding genes have been carried out to investigate the implication of PdPCS and PdMT proteins in Cu homeostasis and/or	its sequestration. Phoenix dactylifera metallothionein induction reached a peak after 30 d of exposure to 0.2 mM of Cu. However, it was down-regulated in plants exposed to higher Cu concentrations. In the same conditions, Pdpcs was overexpressed during 1 mo of exposure before it decreased thereafter. These observations provide a new insight into date palm cell response to Cu, a metal that can be toxic but that is also an essential element.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Chaabene2018a,

title = {Insight into the expression variation of metal-responsive genes in the seedling of date palm (Phoenix dactylifera)},

author = {Zayneb Chaâbene and Agnieszka Rorat and Imen Rekik-Hakim and Fabien Bernard and Grubb-C. Douglas and Amine Elleuch and Franck Vandenbulcke and Hafedh Mejdoub},

year  = {2018},

date = {2018-01-01},

journal = {Chemosphere},

volume = {197},

pages = {123-134},

abstract = {Phytochelatin synthase and metallothionein gene expressions were monitored via qPCR in order to investigate the molecular mechanisms involved in Cd and Cr detoxification in date palm (Phoenix dactylifera). A specific reference gene validation procedure using BestKeeper, NormFinder and geNorm programs allowed selection of the three most stable reference genes in a context of Cd or Cr contamination among six reference gene candidates, namely elongation factor α1, actin, aldehyde dehydrogenase, SAND family, tubulin 6 and TaTa box binding protein. Phytochelatin synthase (pcs) and metallothionein (mt) encoding gene expression were induced from the first days of exposure. At low Cd stress (0.02mM), genes were still up- regulated until 60th day of exposure. At the highest metal concentrations, however, pcs and mt gene expressions decreased. pcs encoding gene was significantly up-regulated under Cr exposure, and was more responsive to increasing Cr concentration than mt encoding gene. Moreover, exposure to Cd or Cr influenced clearly seed germination and hypocotyls elongation. Thus, the results have proved that both analyzed genes participate in metal detoxification and their expression is regulated at transcriptional level in date palm subjected to Cr and Cd stress. Consequently, variations of expression of mt and pcs genes may serve as early-warning biomarkers of metal stress in this species.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Plytycz2018,

title = {The existence of fertile hybrids of closely related model earthworm species, Eisenia andrei and E. fetida},

author = {Barbara Plytycz and Janusz Bigaj and Artur Osikowski and Sebastian Hofman and Andrzej Falniowski and Tomasz Panz and Pawel Grzmil and Franck Vandenbulcke},

year  = {2018},

date = {2018-01-01},

journal = {PLoS ONE},

volume = {13},

number = {1},

pages = {e0191711},

abstract = {Lumbricid earthworms Eisenia andrei (Ea) and E. fetida (Ef) are simultaneous hermaphrodites with reciprocal insemination capable of self-fertilization while the existence of hybridization of these two species was still	debatable. During the present investigation fertile hybrids of Ea	and Ef were detected. Virgin specimens of Ea and Ef were laboratory crossed (Ea+Ef) and their progeny was doubly identified. 1 ±identified	by species-specific maternally derived haploid mitochondrial DNA sequences of the COI gene being either `a' for worms hatched from	Ea ova or `f' for worms hatched from Ef ova. 2 ±identified by the	diploid maternal/ paternal nuclear DNA sequences of 28s rRNA gene	being either `AA' for Ea, `FF' for Ef, or AF/FA for their hybrids derived either from the `aA' or `fF' ova, respectively. Among offspring of Ea+Ef pairs in F1 generation there were mainly aAA and fFF earthworms resulted from the facilitated self-fertilization and some aAF hybrids from aA ova but none fFA hybrids from fF ova. In F2 generation resulting	from aAF hybrids mated with aAA a new generations of aAA and aAF	hybrids were noticed, while aAF hybrids mated with fFF gave fFF and	both aAF and fFA hybrids. Hybrids intercrossed together produced plenty of cocoons but no hatchlings independently whether aAF+aAF or aAF+fFA were mated. These results indicated that Ea and Ef species,	easy to maintain in aboratory and commonly used as convenient models in biomedicine and ecotoxicology, may also serve in studies on molecular basis of interspecific barriers and mechanisms of introgression and speciation. Hypothetically, their asymmetrical hybridization can	be modified by some external factors.},

note = {ACLO},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Lors2018c,

title = {Biodeterioration of mortars exposed to sewers in relation to microbial diversity of biofilms formed on the mortars surface},

author = {Christine Lors and Johanne Aube and Rémy Guyoneaud and Franck Vandenbulcke and Denis Damidot},

year  = {2018},

date = {2018-01-01},

journal = {International Biodeterioration & Biodegradation},

volume = {130},

pages = {23-31},

abstract = {Strong deterioration of concrete in sewer systems is mainly due to 

	microorganisms and especially to sulfur-oxidizing bacteria. Mortars 

	made either with ordinary Portland cement (OPC) or calcium aluminate 

	cement (CAC) were exposed in a waste water collector for five years. 

	Mortar microstructure observed by microscopy reported a larger thickness 

	of the degraded zone for OPC mortar. Taxonomic identification of 

	bacterial communities performed on biofilms collected at the mortar 

	surface reported similar bacterial diversities, but strong differences 

	of relative abundance. A greater neutrophilic sulfur-oxidizing bacterial 

	(NSOB) activity was observed for OPC mortar certainly in conjunction 

	with its larger acid neutralization capacity. Thus, CAC mortar was 

	less biodeteriorated than OPC mortar as less NSOB were able to settle 

	on its surface in relation with its specific microstructure. The 

	results of the reported field experiments have been compared with 

	bioleaching laboratory experiments performed on identical mortars 

	in the presence of Halothiobacillus neapolitanus as NSOB. As the 

	deterioration mechanisms involved were similar, an acceleration factor 

	with respect to the rate of in situ biodeterioration was determined 

	for laboratory experiment.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Beaumelle2017,

title = {Relationships between metal compartmentalization and biomarkers in earthworms exposed to field-contaminated soils},

author = {Léa Beaumelle and Mickaël Hedde and Franck Vandenbulcke and Isabelle Lamy},

year  = {2017},

date = {2017-01-01},

journal = {Environmental Pollution},

volume = {224},

pages = {185-194},

abstract = {Partitioning tissue metal concentration into subcellular compartments reflecting toxicologically available pools may provide good descriptors of the toxicological effects of metals on organisms. Here we investigated the relationships between internal compartmentalization of Cd, Pb and Zn and biomarker responses in a model soil organism: the earthworm. The aim of this study was to identify metal fractions reflecting the toxic pressure in an endogeic, naturally occurring earthworm species (Aporrectodea caliginosa) exposed to realistic field-contaminated soils. After a 21 days exposure experiment to 31 field-contaminated soils, Cd, Pb and Zn concentrations in earthworms and in three subcellular 

 ractions (cytosol, debris and granules) were quantified. Different biomarkers were measured: the expression of a metallothionein gene (mt), the activity of catalase (CAT) and of glutathione-s-transferase (GST), and the protein, lipid and glycogen reserves. Biomarkers were further combined into an integrated biomarker index (IBR). The subcellular fractionation provided better predictors of biomarkers than the total internal contents hence supporting its use when assessing toxicological bioavailability of metals to earthworms. The most soluble internal pools of metals were not always the best predictors of biomarker responses. metallothionein expression responded to increasing concentrations 

 of Cd in the insoluble fraction (debris granules). Protein and glycogen contents were also mainly related to Cd and Pb in the insoluble fraction. On the other hand, GST activity was better explained by Pb in the cytosolic fraction. CAT activity and lipid contents variations were not related to metal subcellular distribution. The IBR was best explained by both soluble and insoluble fractions of Pb and Cd. This study further extends the scope of mt expression as a robust and specific biomarker in an ecologically representative earthworm species exposed to field-contaminated soils. The genetic lineage of the individuals, assessed by DNA barcoding with cytochrome c oxidase subunit I, did not influence mt expression. (C) 2017 Elsevier Ltd. All rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Chaabene2017b,

title = {The phytochelatin synthase gene in date palm (Phoenix dactylifera L.): Phylogeny, evolution and expression},

author = {Zayneb Chaâbene and Hakim-Imen Rekik and Walid Kriaa and C.-Douglas Grubb and Bassem Khemakhem and Franck Vandenbulcke and Hafedh Mejdoub and Amine Elleuch},

year  = {2017},

date = {2017-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {140},

pages = {7-17},

abstract = {We studied date palm phytochelatin synthase type i (pdpcs1), which catalyzes the cytosolic synthesis of phytochelatins (pcs), a heavy metal binding protein, in plant cells. the gene encoding pdpcs1 (pdpcs) consists of 8 exons and 7 introns and encodes a protein of 528 amino acids. pcs gene history was studied using notung phylogeny. during evolution, gene loss from several lineages was predicted including proteobacteria, bilateria and brassicaceae. in addition, eleven gene duplication events appeared toward interior nodes of the reconciled tree and four gene duplication events appeared toward the external nodes. these latter sequences belong to species with a second copy of pcs suggesting that this gene evolved through subfunctionalization. pdpcs1 gene expression was measured in seedling hypocotyls exposed to cd, cu and cr using quantitative real-time polymerase chain reaction (qpcr). a pdpcs1 overexpression was evidenced in p. dactylifera seedlings exposed to metals suggesting that 1-the pdpcs1 gene is functional, 2-there is an implication of the enzyme in metal detoxification mechanisms. additionally, the structure of pdpcs1 was predicted using its homologue from nostoc (cyanobacterium, nspcs) as a template in discovery studio and pymol software. these analyses allowed us to identify the phytochelatin synthase type i enzyme in date palm (pdpcs1) via recognition of key consensus amino acids involved in the catalytic mechanism, and to propose a hypothetical binding and catalytic site for an additional substrate binding cavity.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Courtois2017,

title = {Sewage sludge, silver nanoparticles and terrestrial environments: state of the art and involved work},

author = {Pauline Courtois and Agnieszka Rorat and Sébastien Lemière and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {International Conference of Environmental Biotechnology, 12th December 2017, Czestochowa (Poland)},

abstract = {The fast increase of the global population, urbanization, industrialization and the improvement of technologies used in wastewater treatment plant have led to a strong increase of the production of sewage sludge. because of the high organic matter and nutrients content, sewage sludge can be applied as a fertilizer, but some contaminants shall be strictly monitored. in europe, legislation focused on: seven metal trace elements (cadmium, chromium, copper, mercury, nickel, lead and zinc) and organic traces compounds (7 polychlobiphenyls and 3 polycyclic aromatic hydrocarbons (fluoranthene, benzo(b)fluoranthene and benzo(a)pyrene)). still, some potentially dangerous contaminants, e.g. silver nanoparticles are not regulated at present. due to their biocidal properties, silver nanoparticles are among the most frequently used nanomaterials (in more of 300 products and production estimated around 500 tons per year). releases in the environment through wastewater treatment plants, linked to the life cycle of products containing nano-silver, can cause a contamination of ecosystem and ecotoxicological and/or health effects [1]. many research aimed on the assessment of the risks and dangers associated with the use of silver, nano-silver and their derivatives on organisms and ecosystems. recent works have shown that the nano-silver 1) decrease the diversity and the quantity of soil microorganisms [2], 2) are accumulated in plant and animal tissues [3,4] and 3) cause biological effects on studied organisms. nevertheless, the understanding of their effects on the terrestrial ecosystem is still incomplete and many questions subsist. this work aims on the effects of the nano-silver toxicity in terrestrial environment, mainly throught its effects on organisms living in close contact with soil, to characterize it and to understand its mechanisms of action on organisms. references [1] anses, 2015. avis de l’agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail. [2] schlich k.et hund-rinke, k., 2015. influence of soil properties on the effect of silver nanomaterials on microbial activity in five soils. environmental pollution. 196, 321–330. [3] colman b.p., arnaout c.l., anciaux s., gunsch c.k., hochella jr m.f., kim b., lowry g.v., mcgill b.m., reinsch b.c., c.j. richardson, unrine j.m., wright j.p., yin l. et bernhardt e.s. 2013. low concentrations of silver nanoparticles in biosolids cause adverse ecosystem responses under realistic field scenario. plos one 8(2): e57189. doi: 10.1371/journal.pone.0057189 [4] shoults-wilson, w.a., reinsch, b.c., tsyusko, o.v., bertsch, p.m., lowry, g.v., unrine, j.m., 2011. effect of silver nanoparticle surface coating on bioaccumulation and reproductive toxicity in earthworms ( eisenia fetida ). nanotoxicology 5, 432–444.},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Jaskulak2017a,

title = {Expression of metallothioneins and phytochelatins in Lupinus luteus L. exposed to heavy metals and nanoparticles},

author = {Marta Jaskulak and Aneta Murtaś and Agnieszka Rorat and Anna Grobelak and Zayneb Chaâbene and Małgorzata Kacprzak and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {SETAC North America 38th Annual Meeting, 12-16 November 2017, Minneapolis},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Murtas2017a,

title = {Expression of metallothionein coding gene in Festuca pratensis H. grown on artificially contaminated soils with silver nanoparticles or ionic silver},

author = {Aneta Murtaś and Marta Jaskulak and Agnieszka Rorat and Anna Grobelak and Zayneb Chaâbene and Małgorzata Kacprzak and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {SETAC North America 38th Annual Meeting, 12-16 November 2017, Minneapolis},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Rorat2017b,

title = {ETNA2 project: Silver ecotoxicology in terrestrial environments},

author = {Agnieszka Rorat and Pauline Courtois and Sébastien Lemière and E Attard and Rémy Guyoneaud and C Levard and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {International Conference of Environmental Biotechnology, 12th December 2017, Czestochowa (Poland)},

abstract = {Due to the increasing incorporation of silver nanoparticles (agnp) in consumer products, silver contamination of sewage sludge may increase and have environmental impacts. indeed sewage sludge are sometimes used in agriculture as fertilizers. in such a context, our project etna2 propose to monitor the silver concentrations in selected sewage sludge coming from southern poland during 2 years and examine their evolution in term of concentrations. in parallel, the assessment of the terrestrial ecotoxicity of silver ions, silver nanoparticles (agnp) and their transformation products (sulphides of silver) will be conducted. therefore, such ecotoxicological assessment will be done using key organisms of soil functioning which are affected by chemical contamination: microorganisms and earthworms. exposures of the ecotoxicologically important test species eisenia fetida were conducted in mesocosms containing agricultural reference soil contaminated by sewage sludge, ag ions, agnp or silver transformation products. these compounds were added to the soil or through the sludge after fermentation. our aim is to address the lack of informations concerning the question of silver in soil ecotoxicology. the impact of ag on soil microflora communities was explored as preliminary experiments evidenced the bactericidal properties of ag in this environmental context. a better understanding of the effects of ag, depending on its form (sulphides from wwtp sludge, ag ions and agnps) will be achieved mainly through genomic and trancriptomic approaches combined with full physico-chemical characterization. analysis will be conducted to make a link between silver evolution/ behavior in sludge and soils and their terrestrial ecotoxicity},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Rorat2017c,

title = {Silver and silver nanoparticles as a threat for terrestrial ecosystems},

author = {Agnieszka Rorat and Pauline Courtois and Sébastien Lemière and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {The XXVI International Symposium “Molecular and Physiological Aspects of Regulatory Processes of the Organism, 16 September 2017, Kraków (Poland)},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Rorat2017d,

title = {Evaluation du vermicompostage appliqué aux boues d'épuration par la surveillance de la qualité du compost et des réponses immunitaires de trois espèces de vers de terre: Eisenia fetida, Eisenia andrei et Dendrobaena veneta},

author = {Agnieszka Rorat and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {Colloque 2017 de la Société Française d'Ecotoxicologie Fondamentale et Appliquée, 29-30 juin 2017, Lille.},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Rorat2017d,

title = {Protective role of metallothionein during regeneration in Eisenia andrei exposed to cadmium},

author = {Agnieszka Rorat and Franck Vandenbulcke and Adrianna Gałuszka and Beata Klimek and Barbara Plytycz},

year  = {2017},

date = {2017-01-01},

journal = {Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology},

volume = {203},

pages = {39-50},

abstract = {Lumbricid earthworms are often exposed to simultaneous action of various environmental stressors like soil contamination, temperature fluctuation or predators' attacks, which may induce extrusion of coelomocytecontaining coelomic fluid or loss of tail segments. if the injuries are not lethal, renewal of the immune-competent cells and soluble components of coelomic fluid and/or the regeneration of tail segments occurs. the aim of our investigations was to test the hypothesis that exposure of adult earthworms eisenia andrei to cadmium-polluted soil at room temperature (rt) and/or low temperature (6 °c) have adverse effects on restoration of experimentally depleted coelomocytes or on regeneration of amputated posterior segments. intact control earthworms and their experimental counterparts subjected to electrostimulation-induced coelomocyte depletion or surgical amputation of posterior segments were maintained either in control soil or in soil spiked with cadmium chloride (500 mg/kg air-dried soil) at rt or 6 °c. four weeks after the beginning of experiments, cadmium accumulation in earthworm bodies was significantly lower at 6 °c than at room temperature. the numbers of restored cells and fluorophore contents were hardly affected by temperature or cadmium. however, cocoon production was reduced by cadmium and completely abolished at 6 °c and regeneration of amputated posterior segments was inhibited in cold but was enhanced by cadmium exposure at rt. independently on the temperature, the 4-week cadmium exposure of adult earthworms was connected with significantly upregulated expression of cd-metallothionein (but not of catalase, lysenin and phytochelatin) in coelomocytes},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Rorat2017a,

title = {Vermiremediation of polycyclic aromatic hydrocarbons and heavy metals in sewage sludge composting process},

author = {Agnieszka Rorat and Dariusz Wloka and Anna Grobelak and Anna Grosser and Aleksandra Sosnecka and Marcin Milczarek and Paulina Jelonek and Franck Vandenbulcke and Malgorzata Kacprzak},

year  = {2017},

date = {2017-01-01},

journal = {Journal of Environmental Management},

volume = {187},

pages = {347-353},

abstract = {The main objective of this work was to study the dynamics of the degradation of polycyclic aromatic hydrocarbons (pahs) during sewage sludge vermicomposting. this eco-biotechnology employing earthworms as natural bioreactors for decomposing of organic matter may be used for vermiremediation of particular pollutants present in various organic matter sources. in this experiment, sewage sludge was mixed with bulking agents and precomposted. afterward, adult eisenia andrei specimens were introduced into the process. total heavy metal and pahs concentration were measured in composts and earthworms before and after the process. while heavy metal concentrations fluctuated mildly in the substratum, several metals clearly accumulated in the earthworms' bodies. body accumulation factors could be ranked as follows (cd > cu > zn > ni > cr > pb). interestingly, addition of earthworms into the process has led to the high percentage of pahs removal and some of the 16 priority pahs analyzed in this study have been accumulated in earthworms' bodies. applied conditions did not affect worms' viability but they almost completely inhibited their reproduction.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Suleiman2017,

title = {Determination of the performance of vermicomposting process applied to sewage sludge by monitoring of the compost quality and immune responses in three earthworm species: Eisenia fetida, Eisenia andrei and Dendrobaena veneta},

author = {Hanine Suleiman and Agnieszka Rorat and Anna Grobelak and Anna Grosser and Marcin Milczarek and Barbara Płytycz and Małgorzata Kacprzak and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

journal = {Bioresource Technology},

volume = {241},

pages = {103-112},

abstract = {The aim of this study was to assess the effectiveness of vermicomposting process applied on three different sewage sludge (precomposted with grass clippings, sawdust and municipal solid wastes) using three different earthworm species. selected immune parameters, namely biomarkers of stress and metal body burdens, have been used to biomonitor the vermicomposting process and to assess the impact of contaminants on earthworm’s physiology. biotic and abiotic parameters were also used in order to monitor the process and the quality of the final product. dendrobaena veneta exhibited much lower resistance in all experimental conditions, as the bodyweight and the total number of circulating immune cells decreased in the most contaminated conditions. all earthworm species accumulated heavy metals as follows cd > co > cu > zn > ni > pb > cr: eisenia sp. worms exhibited the highest ability to accumulate several heavy metals. vermicompost obtained after 45 days was acceptable according to agronomic parameters and to compost quality norms in france and poland},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Vandenbulcke2017,

title = {Silver nanoparticles in sewage sludge- a new threat?},

author = {Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {International Conference of Environmental Biotechnology. Ecole Polytechnique de Czestochowa, 12-13 Décember 2017 (Poland)},

note = {INV},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Beaumelle2016,

title = {A structural equation model of soil metal bioavailability to earthworms: confronting causal theory and observations using a laboratory exposure to field-contaminated soils},

author = {Léa Beaumelle and Denis Vile and Isabelle Lamy and Franck Vandenbulcke and Frédéric Gimbert and Mickaël Hedde},

year  = {2016},

date = {2016-01-01},

journal = {Science of the Total Environment},

volume = {569},

pages = {961-972},

abstract = {Structural equation models (sem) are increasingly used in ecology as multivariate analysis that can represent theoretical variables and address complex sets of hypotheses. here we demonstrate the interest of sem in ecotoxicology, more precisely to test the three-step concept of metal bioavailability to earthworms. the sem modeled the three-step causal chain between environmental availability, environmental bioavailability and toxicological bioavailability. in the model, each step is an unmeasured (latent) variable reflected by several observed variables. in an exposure experiment designed specifically to test this sem for cd, pb and zn, aporrectodea caliginosa was exposed to 31 agricultural field-contaminated soils. chemical and biological measurements used included cacl2-extractable metal concentrations in soils, free ion concentration in soil solution as predicted by a geochemical model, dissolved metal concentration as predicted by a semi-mechanistic model, internal metal concentrations in total earthworms and in subcellular fractions, and several biomarkers. the observations verified the causal definition of cd and pb bioavailability in the sem, but not for zn. several indicators consistently reflected the hypothetical causal definition and could thus be pertinent measurements of cd and pb bioavailability to earthworm in field-contaminated soils. sem highlights that the metals present in the soil solution and easily extractable are not the main source of available metals for earthworms. this study further highlights sem as a powerful tool that can handle natural ecosystem complexity, thus participating to the paradigm change in ecotoxicology from a bottom-up to a top-down approach. (c) 2016 elsevier b.v. all rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Bernard2016,

title = {Antioxidant defense gene analysis in Brassica oleracea and Trifolium repens exposed to Cd and/or Pb},

author = {Fabien Bernard and Sylvain Dumez and Franck Brulle and Sébastien Lemière and Anne Platel and Fabrice Nesslany and Damien Cuny and Annabelle Deram and Franck Vandenbulcke},

year  = {2016},

date = {2016-01-01},

journal = {Environmental Science and Pollution Research},

volume = {23},

number = {4},

pages = {3136-3151},

abstract = {This study focused on the expression analysis of antioxidant defense genes in brassica oleracea and in trifolium repens. plants were exposed for 3, 10, and 56 days in microcosms to a field-collected suburban soil spiked by low concentrations of cadmium and/or lead. in both species, metal accumulations and expression levels of genes encoding proteins involved and/or related to antioxidant defense systems (glutathione transferases, peroxidases, catalases, metallothioneins) were quantified in leaves in order to better understand the detoxification processes involved following exposure to metals. it appeared that strongest gene expression variations in t. repens were observed when plants are exposed to cd (metallothionein and ascorbate peroxidase upregulations) whereas strongest variations in b. oleracea were observed in case of cd/pb co-exposures (metallothionein, glutathione transferase, and peroxidase upregulations). results also suggest that there is a benefit to use complementary species in order to better apprehend the biological effects in ecotoxicology},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Rorat2016,

title = {Interactions between sewage sludge-amended soil and earthworms—comparison between Eisenia fetida and Eisenia andrei composting species},

author = {Agnieszka Rorat and Hanine Suleiman and Anna Grobelak and Anna Grosser and Malgorzata Kacprzak and Barbara Plytycz and Franck Vandenbulcke},

year  = {2016},

date = {2016-01-01},

journal = {Environmental Science and Pollution Research},

volume = {23},

number = {4},

pages = {3026-3035},

abstract = {Vermicomposting is an eco-friendly technology, where earthworms are introduced in the waste, inter alia sewage sludge, to cooperate with microorganisms and enhance decomposition of organic matter. the main aims of the present study was to determine the influence of two different earthworm species, eisenia fetida and eisenia andrei, on the changes of selected metallic trace elements content in substratum during vermicomposting process using three different sewage sludge mainly differentiated by their metal contents. final vermicompost has shown a slight reduction in cd, cu, ni, and pb, while the zn concentration tends to increase. accumulation of particular heavy metals in earthworms’ bodies was assessed. both species revealed high tendency to accumulate cd and zn, but not cu, ni, and pb, but e. andrei has higher capabilities to accumulate some metals. riboflavin content, which content varies depending on metal pollution in several earthworms species, was measured supravitaly in extruded coelomocytes. riboflavin content decreased slightly during the first 6 weeks of exposure and subsequently restored till the end of the 9-week experiment. selected agronomic parameters have also been measured in the final product (vermicompost) to assess the influence of earthworms on substratum},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Souleman2016,

title = {Isolation and characterization of eight polymorphic microsatellites markers for the earthworm Lumbricus terrestris},

author = {Dima Souleman and Fabien Grumiaux and Hélène Frérot and Franck Vandenbulcke and Maxime Pauwels},

year  = {2016},

date = {2016-01-01},

journal = {European Journal of Soil Biology},

volume = {74},

pages = {76-80},

abstract = {The earthworm lumbricus terrestris, which is an ecologically important sentinel species for soils widely distributed throughout northern europe, has never been involved in deep population genetic studies. in order to promote future studies, we report here the isolation and characterization of 8 new polymorphic microsatellite loci isolated from sequencing of a microsatellite-enriched genomic library. microsatellite markers were tested on 192 field collected individuals. allelic richness per locus varied from 4.921 to 24.876, with a mean value of 6.125 alleles per locus. the observed heterozygosity (ho) ranged from 0.132 to 0.839, whereas the expected heterozygosity (he) ranged from 0.407 to 0.926. developed markers should be helpful for accurate estimation of population structure, dispersal and gene flow among populations},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Vandenbulcke2016,

title = {Nanoparticles in waste: New threat?},

author = {Franck Vandenbulcke},

year  = {2016},

date = {2016-01-01},

booktitle = {ASSM (Advances in Sustainable Sewage sludge Management) 2016 International Conference, 18-22 September 2016, Cracovie (Pologne)},

note = {INV},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Lanier2016,

title = {Combined effect of Cd and Pb spiked field soils on bioaccumulation, DNA damage, and peroxidase activities in Trifolium repens},

author = {Caroline Lanier and Fabien Bernard and Sylvain Dumez and Julie Leclercq-Dransart and Sébastien Lemière and Franck Vandenbulcke and Fabrice Nesslany and Anne Platel and I Devred and Damien Cuny and Annabelle Deram},

year  = {2016},

date = {2016-01-01},

journal = {Environmental Science and Pollution Research},

volume = {23},

number = {2},

pages = {1755-1767},

abstract = {The present study was designed to investigate the combined effects of cd and pb on accumulation and genotoxic potential in white clover (trifolium repens). for this purpose, t. repens was exposed to contaminated soils (2.5–20 mg kg−1 cadmium (cd), 250–2000 mg kg−1 lead (pb) and a mixture of these two heavy metals) for 3, 10 and 56 days. the resulting bioaccumulation of cd and pb, dna damage (comet assay) and peroxidase activities (apox and gpox) were determined. the exposure time is a determinant factor in experiments designed to measure the influence of heavy metal contamination. the accumulation of cd or pb resulting from exposure to the two-metal mixture does not appear to depend significantly on whether the white clover is exposed to soil containing one heavy metal or both. however, when t. repens is exposed to a cd/pb mixture, the percentage of dna damage is lower than when the plant is exposed to monometallic cd. dna damage is close to that observed in the case of monometallic pb exposure. peroxidase activity cannot be associated with dna damage under these experimental conditions},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Bernard2015,

title = {Antioxidant responses of Annelids, Brassicaceae and Fabaceae to pollutants: A review},

author = {Fabien Bernard and Franck Brulle and Sylvain Dumez and Sébastien Lemière and Anne Platel and Fabrice Nesslany and Damien Cuny and Annabelle Deram and Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {114},

pages = {273-303},

abstract = {Pollutants, such as Metal Trace Elements (MTEs) and organic compounds 

	(polycyclic aromatic hydrocarbons, pesticides), can impact DNA structure 

	of living organisms and thus generate damage. For instance, cadmium 

	is a well-known genotoxic and mechanisms explaining its clastogenicity 

	are mainly indirect: inhibition of DNA repair mechanisms and/or induction 

	of Reactive Oxygen Species (ROS). Animal or vegetal cells use antioxidant 

	defense systems to protect themselves against ROS produced during 

	oxidative stress. Because tolerance of organisms depends, at least 

	partially, on their ability to cope with ROS, the mechanisms of production 

	and management of ROS were investigated a lot in Ecotoxicology as 

	markers of biotic and abiotic stress. This was mainly done through 

	the measurement of enzyme activities The present Review focuses on 

	3 test species living in close contact with soil that are often used 

	in soil ecotoxicology: the worm Eisenia fetida, and two plant species, 

	Trifolium repens (white clover) and Brassica oleracea (cabbage). 

	E. fetida is a soil-dwelling organism commonly used for biomonitoring. 

	T. repens is a symbiotic plant species which forms root nodule with 

	soil bacteria, while B. oleracea is a non-symbiotic plant. In literature, 

	some oxidative stress enzyme activities have already been measured 

	in those species but such analyses do not allow distinction between 

	individual enzyme involvements in oxidative stress. Gene expression 

	studies would allow this distinction at the transcriptomic level. 

 

	A literature review and a data search in molecular database were carried 

	out on the basis of keywords in Scopus, in PubMed and in Genbank™ 

	for each species. Molecular data regarding E. fetida were already 

	available in databases, but a lack of data regarding oxidative stress 

	related genes was observed for T. repens and B. oleracea. By exploiting 

	the conservation observed between species and using molecular biology 

	techniques, we partially cloned missing candidates involved in oxidative 

	stress and in metal detoxification in E. fetida, T. repens and B. 

	oleracea.},

note = {ACLS},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Bernard2015a,

title = {Gene expression analysis of antioxidant responses in Annelids, Brassicaceae and Fabaceae exposed to cadmium and/or lead contaminated soils},

author = {Fabien Bernard and Franck Brulle and Sylvain Dumez and Sébastien Lemière and Anne Platel and Fabrice Nesslany and Damien Cuny and Annabelle Deram and Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

booktitle = {SETAC, 3-7 May 2015, Barcelone (Espagne)},

abstract = {Pollutants, such as Metal Trace Elements (MTEs) and organic compounds 

	(polycyclic aromatic hydrocarbons, pesticides), can impact DNA structure 

	of living organisms and thus generate DNA damage. Animal or vegetal 

	cells use antioxidant defense systems to protect themselves against 

	Reactive Oxygen Species (ROS) produced during oxidative stress. The 

	mechanisms of production and management of ROS were investigated 

	a lot in ecotoxicology as unspecific markers. We performed a literature 

	survey of the biochemical responses (enzyme activities) and expression 

	variations of genes encoding proteins involved and/or related to 

	antioxidant response in three species that are often used in ecotoxicology 

	and that live in close contact with soil: the earthworm, Eisenia 

	fetida, the white clover, Trifolium repens and the cabbage, Brassica 

	oleracea. Literature survey and data search in molecular database 

	were carried out on the basis of keywords in Scopus, in PubMed and 

	in Genbank™ for each species (and in related species). In literature, 

	some antioxidant enzyme activities have already been measured in 

	those species but such analysis do not allow distinction of individual 

	enzyme involvements in oxidative stress. Gene expression studies 

	would allow this distinction at the transcriptomic level. By exploiting 

	the conservation observed between species and using molecular biology 

	techniques, we partially cloned and measured the gene expression 

	of many candidates involved and/or related to antioxidant responses 

	in E. fetida, T. repens and B. oleracea. Therefore, analysis of the 

	gene expression level of most effectors involved and/or related to 

	antioxidant responses has been performed in the 3 species exposed 

	in vivo (3, 10 and 56 days) in a well-characterised sub-urban reference 

	soil spiked with cadmium and/or lead at environmental concentrations. 

	The use of gene expression analysis (in combination with metal accumulation 

	analysis) has shown the interest of studying the effects of metal 

	stress in several species. A major interest of this work lies in 

	the measurement of most genes involved and/or related to antioxidant 

	response at the same time and in several species. Indeed, as responses 

	to oxidative stress may be different between species for the same 

	protein family, one may keep in mind that model species should be 

	used in a complementary manner in order to better understand the 

	effects of xenobiotics on exposed organisms.},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Homa2015,

title = {Dermal exposure of Eisenia andrei earthworms: Effects of heavy metals on metallothionein and phytochelatin synthase gene expressions in coelomocytes},

author = {Joanna Homa and Agnieszka Rorat and Jerzy Kruk and Claude Cocquerelle and Barbara Plytycz and Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

journal = {Environmental Toxicology and Chemistry},

volume = {34},

number = {6},

pages = {1397-1404},

abstract = {Parameters such as total number of coelomocytes, riboflavin content in coelomocytes, expression of genes implied in metal homeostasis, and detoxification mechanisms can be used as biomarkers to assess the impact of metals on annelids. defense biomarkers (detoxification gene expressions and coelomocyte parameters) were investigated in the ecotoxicologically important species eisenia andrei following in vivo exposure to 5 different metals (zinc, copper, nickel, lead, and cadmium) at known concentrations. coelomocyte numbers and riboflavin content were not affected by metallic exposure, but metal-specific gene expression variations were evidenced},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Lemiere2015,

title = {Is Eisenia fetida a suitable species to monitor environmental soil genotoxicants},

author = {Sébastien Lemière and Annabelle Deram and Fabien Bernard and Alain Leprêtre and Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

booktitle = {SETAC, 3-7 May 2015, Barcelone (Espagne)},

abstract = {As well as for air and waters, not only chemical analyses have to be taken into account to monitor anthropic contamination of soils. biological responses of exposed organisms should be considered too and particularly for environmental genotoxicants as pahs or metals. it is well-known that studies of soil contaminations are complex. for example, the environmental availability, environmental and toxicological bioavailabilities of present metal contaminants, influenced by soil factors, ageing phenomena, organic matter..., their concomitant presences, and often at low concentrations and then their potential toxic interactions have to be considered. that’s why methods of classical genetic toxicology (indirect testing, unrealistic exposure...) did not seem fully satisfactory. several years ago, we developed an environmental genotoxicity marker with eisenia fetida,the comet assay. it is a technique allowing the evaluation of dna damage (single and double strand breaks, alkali-labile sites) in a cellular population and it was developed on cœlomocytes of this biological model largely used in soil ecotoxicology. in this poster presentation, we will present obtained results in different contexts with this environmental genotoxicty marker: exposures to field-contaminated soils (1), to spiked soils with a mixture of metals (2) and to soils after a remediation treatment (3). in the first work (1), in vivo short-term exposures of e. fetida were conducted using soils sampled in two different areas in the north of france: from the vicinity of a former smelter and from a suburb zone close to a battery plant still in activity. in the second (2), in order to better understand metal interactions, in vivo short to mid-term exposures (3, 10 and 56 days) were performed using an urban-surrounding soil spiked with cadmium or/and lead at concentrations found in french contaminated soils. in the last work (3), in vivo short-term exposures of e. fetida were conducted using a contaminated soil sampled in the field and a reference soil contaminated or not by a mixture of lead, zinc and cadmium. in both soils were added (or not) remediation agents (biochars from two origins) at two concentrations. in all cases, obtained genotoxicity results after these exposures will be detailed. we will try to discuss the interests and limits of our environmental genotoxicity marker and to conclude if it is useful and relevant to monitor anthropic contaminated soils.},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pauget2015,

title = {Soil bioindicators: how soil properties influence their responses and how to select them in function of the site issues?},

author = {Benjamin Pauget and Laurence Rougé and Antonio Bispo and Cécile Grand and Thierry Béguiristain and Jean-Jacques Bessoule and Josselin Bodilis and Rémi Chaussod and Nathalie Cheviron and Michaël Coeurdassier and Jérôme Cortet and Steven Criquet and Samuel Dequiedt and Olivier Faure and Christophe Gangneux and Isabelle Gattin and Marina Le-Guédard and Adnane Hitmi and Nicolas Laurent and Marc Legras and Sylvie Nélieu and Nuria Ruiz and Salima Taibi and Franck Vandenbulcke and Annette de-Vaufleury and Cécile Villenave and Guénola Pérès},

year  = {2015},

date = {2015-01-01},

booktitle = {SETAC, 3-7 May 2015, Barcelone (Espagne)},

abstract = {Context - despite no directive exists for soil protection, this key component of ecosystem needs to be protected. to fill the lack of tools to monitor the soil threats and to assess the impact of soil management, biological indicators have been developed in the french program bioindicators 2 (ademe). on the 47 plots of 13 sites (agricultural, industrial and forest), the influence of soil characteristics and soil use on the response of 80 biological parameters (fauna, flora and microorganisms) have been assessed by using linear multivariate regressions. results - the soil texture and the organic carbon rate has been identified as the main soil characteristic influencing the biological responses, even if each bioindicator is influenced by its own pool of soil parameters. it underlines the necessity to use battery of bioindicators for an integrative assessment of soil quality. an influence of soil contaminants (organic or metallic) have been highlighted for 86% of the bioindicators showing the necessity to consider their bioavailability for suitable soil management. to disseminate the use of bioindicators, a web interface has been implemented (http://ecobiosoil.univ-rennes1.fr/ademe-bioindicateur/). it presents all the biological methods developed in the program and allows consultation of the variation range of each bioindicator considering site contexts and soil physico-chemical characteristics of soils thus leading to provide a first benchmark. it also provides tools to the end-users to select the bioindicator(s) which best fit(s) with their own site problematic (agricultural practices, bioavailability of contaminants, soil monitoring...) and to communicate (technical sheets...). conclusion – for the first time, the soil properties influence on the response of 80 bioindicators was characterized under similar environmental conditions. as each bioindicator is influenced by a specific pool of soil parameter, chemical measures cannot be used as a surrogate of biological measures. this program has provided usable tools for a biologically-based site management},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Peres2015,

title = {Which bioindicators are suitable for soil quality monitoring and risk assessment? From relevance study to transfer tool development},

author = {Guénola Pérès and Benjamin Pauget and Annette De-Vaufleury and Michaël Coeurdassier and Marina Le-Guédard and Jean-Jacques Bessoule and Samuel Dequiedt and Rémi Chaussod and Lionel Ranjard and Daniel Cluzeau and Muriel Guernion and Laurence Rougé and Mickaël Hedde and Nathalie Cheviron and Jeanne-Chantal Dur and Sylvie Nélieu and Christian Mougin and Isabelle Gattin and Christophe Gangneux and Nicolas Laurent and Marc Legras and Karine Laval and Patrice Lepelletier and Salima Taibi and Cécile Villenave and Olivier Faure and Jennifer Hellal and Jérôme Cortet and Thierry Béguiristain and Corinne Leyval and Josselin Bodilis and Steven Criquet and Adnane Hitmi and Nuria Ruiz and Franck Vandenbulcke and Cécile Grand and Laurence Galsomies and Antonio Bispo},

year  = {2015},

date = {2015-01-01},

booktitle = {SETAC, 3-7 May 2015, Barcelone (Espagne)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Suleiman2015,

title = {Comparing different strategies of vermicomposting},

author = {Hanine Suleiman and Agnieszka Rorat and Barbara Plytycz and Malgorzata Kacprzak and Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

booktitle = {Journée des Doctorants IRePSE, 08 juillet 2015, CERLA - Cité Scientifique - Université Lille 1, Villeneuve d'Ascq},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke2015,

title = {Questionnements sur les aspects environnementaux du nano-argent : une nanoparticule d’origine anthropique déjà présente dans l’environnement?},

author = {Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

booktitle = {Forum Nanoresp. Les usages du nanoargent, 6 mai 2015, Paris},

abstract = {Les matériaux à base d’argent à l’état nanoparticulaire, ou nanoargent, sont utilisés pour leurs propriétés antimicrobiennes dans des textiles, des déodorants, des désinfectants, des cosmétiques, des emballages alimentaires, des lessives, dans des revêtements d’électroménager, des composants électroniques, des pansements servant à prévenir les infections après des brûlures, etc. que sait-on aujourd’hui des propriétés et des effets du nanoargent ? existe-t-il des procédés pour l’intégrer dans des matériaux de façon stable et durable ? peut-on l’associer à d’autres nanomatériaux ? dans quelles conditions peut-il se révéler toxique ? par exemple, la concentration d’argent dans les boues d’épuration présente-t-elle un risque potentiel pour l’environnement via l’épandage de ces boues sur les terres agricoles ? en bref, à qui profite le nanoargent ?},

note = {INV},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke2015a,

title = {Valorisation des boues des stations d’épuration sur les sols agricoles, focus sur une contamination émergente},

author = {Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

booktitle = {ANSES, 22 avril 2015, Paris},

note = {INV},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke2015b,

title = {Ecotoxicologie des nano-objets : le nano-argent, une nanoparticule d’origine anthropique déjà rejetée dans l’environnement?},

author = {Franck Vandenbulcke and Sébastien Lemière},

year  = {2015},

date = {2015-01-01},

booktitle = {Journée thématique "nano-poudres, nano-materiaux, nano-objets: produits du futur, de la molecule au consommateur", Ecole Centrale de Lille, 29 janvier 2015, Lille},

note = {INV},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Brulle2014,

title = {Identification of suitable qPCR reference genes in leaves of Brassica oleracea under abiotic stresses},

author = {Franck Brulle and Fabien Bernard and Franck Vandenbulcke and Damien Cuny and Sylvain Dumez},

year  = {2014},

date = {2014-01-01},

journal = {Ecotoxicology},

volume = {23},

number = {3},

pages = {459-471},

abstract = {Real-time quantitative pcr is nowadays a standard method to study gene expression variations in various samples and experimental conditions. however, to interpret results accurately, data normalization with appropriate reference genes appears to be crucial. the present study describes the identification and the validation of suitable reference genes in brassica oleracea leaves. expression stability of eight candidates was tested following drought and cold abiotic stresses by using three different softwares (bestkeeper, normfinder and genorm). four genes (bolc.tub6, bolc.sand1, bolc.ubq2 and bolc.tbp1) emerged as the most stable across the tested conditions. further gene expression analysis of a drought- and a cold-responsive gene (bolc.dreb2a and bolc.elip, respectively), confirmed the stability and the reliability of the identified reference genes when used for normalization in the leaves of b. oleracea. these four genes were finally tested upon a benzene exposure and all appeared to be useful reference genes along this toxicological condition. these results provide a good starting point for future studies involving gene expression measurement on leaves of b. oleracea exposed to environmental modifications},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Deram2014,

title = {Complémentarité des modèles végétaux et animaux pour le diagnostic de la fraction génotoxique de sols contaminés},

author = {Annabelle Deram and Sébastien Lemière and Fabien Bernard and Franck Marot and Sylvain Dumez and Anne Platel and Fabrice Nesslany and Franck Vandenbulcke and Damien Cuny},

year  = {2014},

date = {2014-01-01},

booktitle = {Colloque des 3èmes Rencontres nationales de la recherche sur les sites et sols pollués, 18-19 novembre 2014, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pauget2014,

title = {Soil bioindicators: how soil properties influence their responses and to select them in function of the site issues?},

author = {Benjamin Pauget and Laurence Rougé and Antonio Bispo and Cécile Grand and Thierry Béguiristain and Jean-Jacques Bessoule and Josselin Bodilis and Rémi Chaussod and Nathalie Cheviron and Michaël Coeurdassier and Jérôme Cortet and Steven Criquet and Samuel Dequiedt and Olivier Faure and Christophe Gangneux and Isabelle Gattin and Marina Le-Guédard and Adnane Hitmi and Nicolas Laurent and Marc Legras and Sylvie Nélieu and Nuria Ruiz and Salima Taïbi and Franck Vandenbulcke and Annette De-Vaufleury and Cécile Villenave and Guénola Pérès},

year  = {2014},

date = {2014-01-01},

booktitle = {First Global Soil Biodiversity Conference - Assessing soil biodiversity and its role for ecosystem services, 2-5 décembre 2014, Dijon},

abstract = {Introduction despite no european directive exists for soil protection, this key component of ecosystem needs to be protected. to fill the lack of tools to monitor the soil threats and to assess the impact of soil management, biological indicators have been developed in the french program bioindicators 2 (ademe) and a website allowing selection of relevant bioindicators depending on the question asked achieved. on the 47 plots of 13 sites (agricultural, industrial and forest), the influence of soil characteristics and soil use on the response of 80 biological parameters (fauna, flora and microorganisms) have been assessed by using linear multivariate regressions.},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pauget2014a,

title = {Les indicateurs de l'état biologique des sols: Guide de sélection selon le type d’application et/ou la problématique},

author = {Benjamin Pauget and Laurence Rougé and Guénola Pérès and Antonio Bispo and Cécile Grand and Thierry Béguiristain and Jean-Jacques Bessoule and Josselin Bodilis and Rémi Chaussod and Nathalie Cheviron and Michaël Coeurdassier and Jérôme Cortet and Stéven Criquet and Samuel Dequiedt and Olivier Faure and Christophe Gangneux and Isabelle Gattin and Marina Le-Guédard and Adnane Hitmi and Nicolas Laurent and Marc Legras and Sylvie Nélieu and Nuria Ruiz and Franck Vandenbulcke and Cécile Villenave and Annette De-Vaufleury},

year  = {2014},

date = {2014-01-01},

booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris},

abstract = {Pour surveiller, caractériser et évaluer les menaces pesant sur les sols et évaluer l’impact des méthodes de gestion et de protection de cette ressource essentielle, des outils de bioindication basés sur les propriétés biologiques des sols ont été mis en oeuvre simultanément dans le cadre du programme bioindicateurs 2 (ademe). l'influence des paramètres des sols et de ses contaminants sur les réponses de 80 paramètres biologiques (faune, flore et microbiens) a été évaluée sur 47 modalités de 13 sites (agricoles, forestiers et sites contaminés). l'étude a permis d'identifier les principales caractéristiques physico-chimiques ux paramètres de texture des sols influençant la réponse des bio indicateurs et montre que ces paramètres sont variables selon le bioindicateur considéré. une forte influence des contaminants, qu'ils soient organiques ou métalliques est également observée, ce qui souligne la nécessité de prendre en compte leur biodisponibilité lors de l'établissement la réalsiation de plan de gestiondes études de risque. pour faciliter l'utilisation des bioindicateurs, une interface web basée sur des critères de sélection d’outils choisis avec leurs utilisateurs potentiels a été mise en place. cette interface présente l’ensemble des méthodes outils biologiques appliquées dans le programme bioindicateurs 2 et permet (i) de consulter les gammes de variation des bioindicateurs dans les différents contextes du programme bioindicateurs 2 à la base de ce 1er référentiel d'interprétation des bioindicateurs et (ii) de sélectionner le bioindicateur correspondant le mieux à sa problématique (impact d’une méthode de dépollution par ex.) sur la base de critères scientifiques, et techniques et économiques.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}