Membre

@article{courtois:hal-03499742,

title = {Transfer of sulfidized silver from silver nanoparticles, in sewage sludge, to plants and primary consumers in agricultural soil environment},

author = {Pauline Courtois and Annette Vaufleury and Anna Grosser and Christine Lors and Franck Vandenbulcke},

url = {https://hal-univ-fcomte.archives-ouvertes.fr/hal-03499742},

doi = {10.1016/j.scitotenv.2021.145900},

year  = {2021},

date = {2021-07-01},

urldate = {2021-07-01},

journal = {Science of the Total Environment},

volume = {777},

pages = {145900},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{jaskulak:hal-03493603,

title = {Species-specific Cd-detoxification mechanisms in lumbricid earthworms Eisenia andrei, Eisenia fetida and their hybrids},

author = {Marta Jaskulak and Agnieszka Rorat and Ligia Kurianska-Piatek and Sebastian Hofman and Janusz Bigaj and Franck Vandenbulcke and Barbara Plytycz},

url = {https://hal.archives-ouvertes.fr/hal-03493603},

doi = {10.1016/j.ecoenv.2020.111425},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {208},

pages = {111425 -},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{courtois:hal-03149939,

title = {Medium-term effects of Ag supplied directly or via sewage sludge to an agricultural soil on Eisenia fetida earthworm and soil microbial communities},

author = {Pauline Courtois and Agnieszka Rorat and Sébastien Lemiere and Remy Guyoneaud and Eléonore Attard and Manon Longepierre and Franc cois Rigal and Clément Levard and Perrine Chaurand and Anna Grosser and Anna Grobelak and Malgorzata Kacprzak and Christine Lors and Agnes Richaume and Franck Vandenbulcke},

url = {https://hal.archives-ouvertes.fr/hal-03149939},

doi = {10.1016/j.chemosphere.2020.128761},

year  = {2021},

date = {2021-04-01},

urldate = {2021-04-01},

journal = {Chemosphere},

volume = {269},

pages = {128761},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{grosser:hal-03175993,

title = {Effects of silver nanoparticles on performance of anaerobic digestion of sewage sludge and associated microbial communities},

author = {Anna Grosser and Anna Grobelak and Agnieszka Rorat and Pauline Courtois and Franck Vandenbulcke and Sébastien Lemi`ere and Remy Guyoneaud and Eléonore Attard and Piotr Celary},

url = {https://hal.archives-ouvertes.fr/hal-03175993},

doi = {10.1016/j.renene.2021.02.127},

year  = {2021},

date = {2021-06-01},

urldate = {2021-06-01},

journal = {Renewable Energy},

volume = {171},

pages = {1014-1025},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Audusseau2020,

title = {Impacts of metallic trace elements on an earthworm community in an urban wasteland: Emphasis on the bioaccumulation and genetic characteristics in Lumbricus castaneus},

author = {Hélène Audusseau and Franck Vandenbulcke and Cassandre Dume and Valentin Deschins and Maxime Pauwels and Agnès Gigon and Matthieu Bagard and Lise Dupont},

year  = {2020},

date = {2020-01-01},

journal = {Science of the Total Environment},

volume = {718},

pages = {9},

abstract = {Metallic trace elements (MTEs) soil pollution has become a worldwide 

	concern, particularly regarding its impact on earthworms. Earthworms, 

	which constitute the dominant taxon of soil macrofauna in temperate 

	regions and are crucial ecosystem engineers, are in direct contact 

	with MTEs. The impacts of MTE exposure on earthworms, however, vary 

	by species, with some able to cope with high levels of contamination. 

	We combined different approaches to study the effects of MTEs at 

	different levels of biological organisation of an earthworm community, 

	in a contaminated urban wasteland. Our work is based on field collection 

	of soil and earthworm samples, with a total of 891 adult earthworms 

	from 8 species collected, over 87 quadrats across the study plot. 

	We found that MTE concentrations are highly structured at the plot 

	scale and that some elements, such as Pb, Zn, and Cu. are highly 

	correlated. Comparing species assemblage to MTE concentrations, we 

	found that the juvenile and adult abundances, and community composition, 

	were significantly affected by pollution. Along the pollution gradient, 

	as species richness decreased. Lumbricus castaneus became more dominant. 

	We thus investigated the physiological response of this species to 

	a set of specific elements (Pb, Zn, Cu, and Cd) and studied the impacts 

	of MTE concentrations at the plot scale on its population genetic. 

	These analyses revealed that L castaneus is able to bioaccumulate 

	high quantities of Cd and Zn, but not of Cu and Pb. The population 

	genetic analysis, based on the genotyping of 175 individuals using 

	8 microsatellite markers, provided no evidence of the role of the 

	heterogeneity in MTE concentrations as a barrier to gene flow. The 

	multidisciplinary approach we used enabled us to reveal the comparatively 

	high tolerance of L castaneus to MTE concentrations, suggesting that 

	this is a promising model to study the molecular bases of MTE tolerance. 

	(C) 2020 Elsevier B.V. All rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Courtois2020,

title = {Accumulation, speciation and localization of silver nanoparticles in the earthworm Eisenia fetida},

author = {Pauline Courtois and Agnieszka Rorat and Sébastien Lemiere and Clément Levard and Perrine Chaurand and Anna Grobelak and Christine Lors and Franck Vandenbulcke},

year  = {2020},

date = {2020-01-01},

journal = {Environmental Science and Pollution Research},

pages = {10},

abstract = {The use of silver nanoparticles (AgNPs) in agriculture and many consumer 

	products has led to a significant release of Ag in the environment. 

	Although Ag toxicity in terrestrial organisms has been studied extensively, 

	very little is known about the accumulation capacity and coping mechanisms 

	of organisms in Ag-contaminated soil. In this context, we exposed 

	Eisenia fetida earthworms to artificial OECD soil spiked with a range 

	of concentrations of Ag (AgNPs or AgNO3). The main aims were to (1) 

	identify the location and form of accumulation of Ag in the exposed 

	earthworms and (2) better understand the physiological mechanisms 

	involved in Ag detoxification. The results showed that similar doses 

	of AgNPs or AgNO3 did not have the same effect on E. fetida survival. 

	The two forms of Ag added to soil exhibited substantial differences 

	in speciation at the end of exposure, but the Ag speciation and content 

	of Ag in earthworms were similar, suggesting that biotransformation 

	of Ag occurred. Finally, 3D images of intact earthworms obtained 

	by X-ray micro-computed tomography revealed that Ag accumulated preferentially 

	in the chloragogen tissue, coelomocytes, and nephridial epithelium. 

	Thus, E. fetida bioaccumulates Ag, but a regulation mechanism limits 

	its impact in a very efficient manner. The location of Ag in the 

	organism, the competition between Ag and Cu, and the speciation of 

	internal Ag suggest a link between Ag and the thiol-rich proteins 

	that are widely present in these tissues, most probably metallothioneins, 

	which are key proteins in the sequestration and detoxification of 

	metals.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Jaskulak2020,

title = {Modelling assisted phytoremediation of soils contaminated with heavy metals - Main opportunities, limitations, decision making and future prospects},

author = {Marta Jaskulak and Anna Grobelak and Franck Vandenbulcke},

year  = {2020},

date = {2020-01-01},

journal = {Chemosphere},

volume = {249},

pages = {126196},

abstract = {The heavy metals (HMs) soils contamination is a growing concern since 

	HMs are not biodegradable and can accumulate in all living organisms 

	causing a threat to plants and animals, including humans. Phytoremediation 

	is a cost-efficient technology that uses plants to remove, transform 

	or detoxify contaminants. In recent years, phytoremediation is entering 

	the stage of large-scale modelling via various mathematical models. 

	Such models can be useful tools to further our understanding and 

	predicting of the processes that influence the efficiency of phytoremediation 

	and to precisely plan such actions on a large-scale. When dealing 

	with extremely complicated and challenging variables like the interactions 

	between the climate, soil and plants, modelling before starting an 

	operation can significantly reduce the time and cost of such process 

	by granting us an accurate prediction of possible outcomes. Research 

	on the applicability of different modelling approaches is ongoing 

	and presented work compares and discusses available models in order 

	to point out their specific strengths and weaknesses in given scenarios. 

	The main aim of this paper is to critically evaluate the main advantages 

	and limitations of available models for large-scale phytoremediation 

	including, among others, the Decision Support System (DSS), Response 

	Surface Methodology (RSM), BALANS, PLANTIX and various regression 

	models. Study compares their applicability and highlight existing 

	gaps in current knowledge with a special reference to improving the 

	efficiency of large-scale phytoremediation of sites contaminated 

	with heavy-metals. The presented work can serve as a useful tool 

	when choosing the most suitable model for the phytoremediation of 

	contaminated sites. (C) 2020 Elsevier Ltd. All rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Jaskulak2020a,

title = {Effects of sewage sludge supplementation on heavy metal accumulation and the expression of ABC transporters in Sinapis alba L. during assisted phytoremediation of contaminated sites},

author = {Marta Jaskulak and Anna Grobelak and Franck Vandenbulcke},

year  = {2020},

date = {2020-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {197},

pages = {12},

abstract = {ATP binding cassette (ABC) transporters, types C, G, and B were monitored 

	via qPCR in order to investigate the influence of heavy metal (HM) 

	contamination of post-industrial and post-agricultural soils and 

	the effects of its supplementation with sewage sludge, on Sinapis 

	alba plants. Five house-keeping genes were selected and validated 

	to ensure the best reference points. The relative expression of ABC 

	types C and G genes was profoundly affected by experimental conditions 

	and included their upregulation after plants exposure to heavy metals 

	and downregulation after supplementation with sewage sludge. However, 

	ABC type C was more responsive then type G. The experimental conditions 

	altered the expression of ABC type C gene faster than ABC type G 

	and thus, the expression of ABC type C can therefore potentially 

	be used as a bioindicator during assisted phytoremediation of degraded 

	sites. In clean soil, supplementation with sewage sludge with a slight 

	content of heavy metals still caused an upregulation in the expression 

	of ABC types C and G, which showed that proper toxicity assessments 

	are necessary to ensure safe application of sewage sludge into soils. 

	Results showed that the analysed genes take a significant part in 

	plants metal detoxification and that their expression is regulated 

	at transcriptional level after exposure to soil contaminated with 

	heavy metals by both, industrial activities and by sewage sludge 

	supplementation. Thus, their expression can potentially be used as 

	an early-warning biomarker when soil supplementation with sewage 

	sludge is incorporated into the soil-management process.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Jaskulak2020c,

title = {Modeling and optimizing the removal of cadmium by Sinapis alba L. from contaminated soil via Response Surface Methodology and Artificial Neural Networks during assisted phytoremediation with sewage sludge},

author = {Marta Jaskulak and Anna Grobelak and Franck Vandenbulcke},

year  = {2020},

date = {2020-01-01},

journal = {International Journal of Phytoremediation},

volume = {22},

number = {12},

pages = {1321-1330},

abstract = {The study was aimed to model and optimize the removal of cadmium from 

	contaminated post-industrial soil viaSinapis albaL. by comparing 

	two modeling approaches: Response Surface Methodology (RSM) and Artificial 

	Neural Networks (ANN). The experimental design was done using the 

	Box-Behnken Design method. In the RSM model, the quadratic model 

	was shown to predict the closest results in comparison to our experimental 

	data. For ANN approach, a two-layer Feed-Forward Back-Propagation 

	Neural Network model was designed. The results showed that sewage 

	sludge supplementation increased the efficiency of theSinapis albaplant 

	in removing Cd from the soil. After 28 days of exposure, the removal 

	rate varied from 10.96% without any supplementation to 65.9% after 

	supplementation with the highest possible (law allowed) dose of sewage 

	sludge. The comparison proved that the prediction capability of the 

	ANN model was much higher than that of the RSM model (adjustedR-square: 

	0.98, standard error of the Cd prediction removal: 0.85 +/- 0.02). 

	Thus, the ANN model could be used for the prediction of heavy metal 

	removal during assisted phytoremediation with sewage sludge. Moreover, 

	such approach could also be used to determinate the dose of sewage 

	sludge that will ensure highest process efficiency.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Lanier2019,

title = {Combined toxic effects and DNA damage to two plant species exposed to binary metal mixtures (Cd/Pb)},

author = {Caroline Lanier and Fabien Bernard and Sylvain Dumez and Julie Leclercq-Dransart and Sébastien Lemière and Franck Vandenbulcke and Fabrice Nesslany and Anne Platel and Inès Devred and Audrey Hayet and Damien Cuny and Annabelle Deram},

year  = {2019},

date = {2019-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {167},

pages = {278-287},

abstract = {Acute and long-term (3-, 10- and 56-day exposure) laboratory toxicity 

	tests were carried out to assess the individual and combined toxic 

	effects of cadmium (Cd) and lead (Pb) in Brassica oleracea and Trifolium 

	repens. In addition to morphological parameters, this work also used 

	comet assay to address endpoints in relation to genotoxicity. Bioaccumulation 

	was measured to demonstrate the influence of the mixture on the concentrations 

	of each metal in the plant. 

 

	The statistical method reported by Ince et al. (1999) was used to 

	evaluate the types of interaction between Cd and Pb in each treatment 

	and concerning their combined effect. 

 

	This study concludes that the combined effects of binary metal combinations 

	of Cd/Pb on morphological parameters are most often additive, sometimes 

	antagonistic and more rarely synergistic, thus extending the findings 

	of previous publications on this subject. 

 

	DNA damage analysis revealed concentration- and time-dependent interactions. 

	Synergistic effects of mixed metals (more breaks than individually 

	applied metals) are observed in T. repens after a short exposure. 

	Antagonistic effects are statistically significant after 10 days-exposure, 

	suggesting competition between metals. At 56 days, the rate of DNA 

	damage observed in plants exposed to the Cd/Pb mixture was similar 

	to that measured in plants exposed to lead only and was significantly 

	lower than the rate of DNA damage induced by Cd. This supports the 

	idea that there may be competition between metals and also strengthens 

	the hypothesis that long-term reparation mechanisms may be implemented. 

	Cd/Pb co-exposure does not significantly influence the bioaccumulation 

	of each metal. It is nevertheless important to note that a statistically 

	significant ‘interaction’ is not necessarily biologically relevant 

	and should therefore be considered with caution when assessing heavy 

	metals combined effects},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Courtois2019,

title = {Ecotoxicology of silver nanoparticles and their derivatives introduced in soil with or without sewage sludge: A review of effects on microorganisms, plants and animals},

author = {Pauline Courtois and Agnieszka Rorat and Sébastien Lemiere and Remy Guyoneaud and Eleonore Attard and Clément Levard and Franck Vandenbulcke},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Pollution},

volume = {253},

pages = {578-598},

abstract = {Silver nanoparticles (AgNPs) are widely incorporated in many products, 

	partly due to their antimicrobial properties. The subsequent discharge 

	of this form of silver into wastewater leads to an accumulation of 

	silver species (AgNPs and derivatives resulting from their chemical 

	transformation), in sewage sludge. As a result of the land application 

	of sewage sludge for agricultural or remediation purposes, soils 

	are the primary receiver media of silver contamination. Research 

	on the long-term impact of AgNPs on the environment is ongoing, and 

	this paper is the first review that summarizes the existing state 

	of scientific knowledge on the potential impact of silver species 

	introduced into the soil via sewage sludge, from microorganisms to 

	earthworms and plants. Silver species can easily enter cells through 

	biological membranes and affect the physiology of organisms, resulting 

	in toxic effects. In soils, exposure to AgNPs may change microbial 

	biomass and diversity, decrease plant growth and inhibit soil invertebrate 

	reproduction. Physiological, biochemical and molecular effects have 

	been documented in various soil organisms and microorganisms. Negative 

	effects on organisms of the dominant form of silver in sewage sludge, 

	silver sulfide (Ag2S), have been observed, although these effects 

	are attenuated compared to the effects of metallic AgNPs. However, 

	silver toxicity is complex to evaluate and much remains unknown about 

	the ecotoxicology of silver species in soils, especially with respect 

	to the possibility of transfer along the trophic chain via accumulation 

	in plant and animal tissues. Critical points related to the hazards 

	associated with the presence of silver species in the environment 

	are described, and important issues concerning the ecotoxicity of 

	sewage sludge applied to soil are discussed to highlight gaps in 

	existing scientific knowledge and essential research directions for 

	improving risk assessment. (C) 2019 Elsevier Ltd. All rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Dupont2019,

title = {Genetic variation of the epigeic earthworm Lumbricus castaneus populations in urban soils of the Paris region (France) revealed using eight newly developed microsatellite markers},

author = {Lise Dupont and Maxime Pauwels and Cassandre Dume and Valentin Deschins and Hélène Audusseau and Agnès Gigon and Florence Dubs and Franck Vandenbulcke},

year  = {2019},

date = {2019-01-01},

journal = {Applied Soil Ecology},

volume = {135},

pages = {33-37},

abstract = {Urban soils are subject to intense environmental pressures, e.g. physical 

	disturbance, surface transformation and pollution, which greatly 

	impact the activity of soil organisms. The epigeic earthworm Lumbricus 

	castaneus (Savigny, 1826) is known to be tolerant to trace elements 

	contamination of soils and was found to be abundant in the urban 

	context. In order to investigate how urban environmental stressors 

	shape the population genetic variation of this species, we developed 

	8 microsatellite loci from a microsatellite-enriched genomic library. 

	Polymorphism was explored in 6 populations (153 individuals) from 

	the Paris region. The number of alleles per locus varied from 7 to 

	29. The average expected heterozygosity within populations ranged 

	from 0.596 to 0.705. These new microsatellite markers revealed a 

	significant genetic structure at fine spatial scale, highlighting 

	that they could be particularly useful for genetic studies of populations 

	in urban soils.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Jaskulak2019,

title = {Gene expression, DNA damage and other stress markers in Sinapis alba L. exposed to heavy metals with special reference to sewage sludge application on contaminated sites},

author = {Marta Jaskulak and Anna Grobelak and Anna Grosser and Franck Vandenbulcke},

year  = {2019},

date = {2019-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {181},

pages = {508-517},

abstract = {Bioindicators are promising tools used to detect the long-term effects 

	of selected biosolids on plants development and should be implemented 

	before large-scale supplementation of sewage sludge into the soil. 

	The presented study shows the impact of sewage sludge application 

	on metal-sensitive toxicity biological parameters (biomarkers) in 

	Sinapis alba including: germination, root length, the activity of 

	guaiacol peroxidase, the chlorophyll content, the level of DNA damage 

	and the expression level of Ribulose-1,5-bisphosphate carboxylase/oxygenase 

	(rbcL) and metallothionein (mt). We evaluated data from selected 

	biomarkers in order to broaden our understanding of plants defense 

	mechanisms against heavy metal contamination and the application 

	of sewage sludge into soils. Overall, in contaminated soil after 

	supplementation with both municipal sewage sludges, an increase in 

	toxicity was noticed in DNA damage, mt and rbcl expression and total 

	chlorophyll content. The supplementation of both soils with municipal 

	sewage sludge caused a two-time induction in the mt expression. Moreover, 

	clean soil supplemented with sewage sludge caused an increase in 

	DNA damage shown as the tail moment from approximately 12 mu m on 

	control to 40 mu m after supplementation. Even if those biosolids 

	increased the initial germination, roots length, and biomass in comparison 

	to the unamended soil, the toxicity was evidenced with other stress 

	markers. Results showed, that in order to accurately assess the influence 

	of sewage sludge application on plants the use of several specific 

	biomarkers is required for safe land restoration. The conducted study 

	also confirmed, both under biochemical and genotoxic tests, that 

	iron enrichment for biosolids or contaminated soil can significantly 

	reduce the bioavailability and toxicity of other metals.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Jaskulak2019a,

title = {Bioaccumulation, antioxidative response, and metallothionein expression in Lupinus luteus L. exposed to heavy metals and silver nanoparticles},

author = {Marta Jaskulak and Agnieszka Rorat and Anna Grobelak and Zayneb Chaabene and Malgorzata Kacprzak and Franck Vandenbulcke},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Science and Pollution Research},

volume = {26},

number = {16},

pages = {16040-16052},

abstract = {Yellow-lupin (Lupinus luteus L.) was grown on soils contaminated with 

	heavy metals during two parallel studies. In the first one, the soil 

	was contaminated by industrial activities whereas, in the second 

	one, the soil was artificially contaminated with a single metal including 

	Cd, Pb, Zn, Ni (in nitrate form), and Ag (in nitrate and nanoparticles 

	form). The study was performed to assess a plant's response to contamination 

	including its antioxidative response and molecular mechanisms involved 

	in metal detoxification through the expression level of metallothioneins 

	(MTs). Overall, the study provided insights into identification and 

	validation of housekeeping genes (HKG) in L. luteus under exposure 

	to metal stress and showed the effects of selected heavy metals and 

	silver nanoparticles on the expression of metallothioneins, the activity 

	of guaiacol peroxidase (GPX) and bioaccumulation of metals in leaves 

	of L. luteus. As such, HKG validation using BestKeeper, NormFinder, 

	and geNorm software allowed for the selection of four most stable 

	reference genes in a context metal contamination for the selected 

	plant. Moreover, a significant increase in the expression levels 

	of MT was observed in plants grown under heavy metal stress and none 

	on plants grown on 25mgkg(-1) of silver nanoparticles. Also, the 

	GPX activity and MT expression showed statistically significant changes 

	between different conditions and doses which means that they can 

	be used as highly sensitive stress markers for planning the phytoremediation 

	process on a large scale.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Chaabene2018,

title = {Copper toxicity and date palm (Phoenix dactylifera) seedling tolerance: Monitoring of related biomarker},

author = {Zayneb Chaâbene and Imen Rekik-Hakim and Agnieszka Rorat and Amine Elleuch and Hafedh Mejdoub and Franck Vandenbulcke},

year  = {2018},

date = {2018-01-01},

journal = {Environmental Toxicology and Chemistry},

volume = {Article in Press. DOI: 10.1002/etc.4007},

abstract = {Date palm (Phoenix dactylifera) seeds were exposed to different copper (Cu) solutions to examine plant stress responses. Low Cu concentrations (0.02 and 0.2 mM) caused an increase of seed germination, whereas higher Cu amounts (2 mM) significantly inhibited seed germination, delayed hypocotyl elongation, increased seedling mortality, and reduced the germination index by more than 90%. Metal-related toxicity symptoms appeared after 15 d of 2 mM of Cu exposure. Biochemical activities such as amylase activity and redox balance elements were examined to study the relationship between external Cu amount and internal plant response. The present study showed that amylolytic activity	was dose- and time-dependent. Likewise, H2 O2 production increased after exposure to Cu, which was correlated with thiobarbituric acid reactive substance (TBARS) accumulation. Furthermore at low Cu concentrations, superoxide dismutase (SOD) and catalase (CAT) activities increased, suggesting that date palm seed stimulated its metal homeostasis networks.	However, the highest cupric ion amounts increased cell oxidant accumulation and reduced enzyme production. Gene expression level measures of P. dactylifera phytochelatin synthase (Pdpcs) and P. dactylifera metallothionein (Pdmt) encoding genes have been carried out to investigate the implication of PdPCS and PdMT proteins in Cu homeostasis and/or	its sequestration. Phoenix dactylifera metallothionein induction reached a peak after 30 d of exposure to 0.2 mM of Cu. However, it was down-regulated in plants exposed to higher Cu concentrations. In the same conditions, Pdpcs was overexpressed during 1 mo of exposure before it decreased thereafter. These observations provide a new insight into date palm cell response to Cu, a metal that can be toxic but that is also an essential element.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Chaabene2018a,

title = {Insight into the expression variation of metal-responsive genes in the seedling of date palm (Phoenix dactylifera)},

author = {Zayneb Chaâbene and Agnieszka Rorat and Imen Rekik-Hakim and Fabien Bernard and Grubb-C. Douglas and Amine Elleuch and Franck Vandenbulcke and Hafedh Mejdoub},

year  = {2018},

date = {2018-01-01},

journal = {Chemosphere},

volume = {197},

pages = {123-134},

abstract = {Phytochelatin synthase and metallothionein gene expressions were monitored via qPCR in order to investigate the molecular mechanisms involved in Cd and Cr detoxification in date palm (Phoenix dactylifera). A specific reference gene validation procedure using BestKeeper, NormFinder and geNorm programs allowed selection of the three most stable reference genes in a context of Cd or Cr contamination among six reference gene candidates, namely elongation factor α1, actin, aldehyde dehydrogenase, SAND family, tubulin 6 and TaTa box binding protein. Phytochelatin synthase (pcs) and metallothionein (mt) encoding gene expression were induced from the first days of exposure. At low Cd stress (0.02mM), genes were still up- regulated until 60th day of exposure. At the highest metal concentrations, however, pcs and mt gene expressions decreased. pcs encoding gene was significantly up-regulated under Cr exposure, and was more responsive to increasing Cr concentration than mt encoding gene. Moreover, exposure to Cd or Cr influenced clearly seed germination and hypocotyls elongation. Thus, the results have proved that both analyzed genes participate in metal detoxification and their expression is regulated at transcriptional level in date palm subjected to Cr and Cd stress. Consequently, variations of expression of mt and pcs genes may serve as early-warning biomarkers of metal stress in this species.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Plytycz2018,

title = {The existence of fertile hybrids of closely related model earthworm species, Eisenia andrei and E. fetida},

author = {Barbara Plytycz and Janusz Bigaj and Artur Osikowski and Sebastian Hofman and Andrzej Falniowski and Tomasz Panz and Pawel Grzmil and Franck Vandenbulcke},

year  = {2018},

date = {2018-01-01},

journal = {PLoS ONE},

volume = {13},

number = {1},

pages = {e0191711},

abstract = {Lumbricid earthworms Eisenia andrei (Ea) and E. fetida (Ef) are simultaneous hermaphrodites with reciprocal insemination capable of self-fertilization while the existence of hybridization of these two species was still	debatable. During the present investigation fertile hybrids of Ea	and Ef were detected. Virgin specimens of Ea and Ef were laboratory crossed (Ea+Ef) and their progeny was doubly identified. 1 ±identified	by species-specific maternally derived haploid mitochondrial DNA sequences of the COI gene being either `a' for worms hatched from	Ea ova or `f' for worms hatched from Ef ova. 2 ±identified by the	diploid maternal/ paternal nuclear DNA sequences of 28s rRNA gene	being either `AA' for Ea, `FF' for Ef, or AF/FA for their hybrids derived either from the `aA' or `fF' ova, respectively. Among offspring of Ea+Ef pairs in F1 generation there were mainly aAA and fFF earthworms resulted from the facilitated self-fertilization and some aAF hybrids from aA ova but none fFA hybrids from fF ova. In F2 generation resulting	from aAF hybrids mated with aAA a new generations of aAA and aAF	hybrids were noticed, while aAF hybrids mated with fFF gave fFF and	both aAF and fFA hybrids. Hybrids intercrossed together produced plenty of cocoons but no hatchlings independently whether aAF+aAF or aAF+fFA were mated. These results indicated that Ea and Ef species,	easy to maintain in aboratory and commonly used as convenient models in biomedicine and ecotoxicology, may also serve in studies on molecular basis of interspecific barriers and mechanisms of introgression and speciation. Hypothetically, their asymmetrical hybridization can	be modified by some external factors.},

note = {ACLO},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Lors2018c,

title = {Biodeterioration of mortars exposed to sewers in relation to microbial diversity of biofilms formed on the mortars surface},

author = {Christine Lors and Johanne Aube and Rémy Guyoneaud and Franck Vandenbulcke and Denis Damidot},

year  = {2018},

date = {2018-01-01},

journal = {International Biodeterioration & Biodegradation},

volume = {130},

pages = {23-31},

abstract = {Strong deterioration of concrete in sewer systems is mainly due to 

	microorganisms and especially to sulfur-oxidizing bacteria. Mortars 

	made either with ordinary Portland cement (OPC) or calcium aluminate 

	cement (CAC) were exposed in a waste water collector for five years. 

	Mortar microstructure observed by microscopy reported a larger thickness 

	of the degraded zone for OPC mortar. Taxonomic identification of 

	bacterial communities performed on biofilms collected at the mortar 

	surface reported similar bacterial diversities, but strong differences 

	of relative abundance. A greater neutrophilic sulfur-oxidizing bacterial 

	(NSOB) activity was observed for OPC mortar certainly in conjunction 

	with its larger acid neutralization capacity. Thus, CAC mortar was 

	less biodeteriorated than OPC mortar as less NSOB were able to settle 

	on its surface in relation with its specific microstructure. The 

	results of the reported field experiments have been compared with 

	bioleaching laboratory experiments performed on identical mortars 

	in the presence of Halothiobacillus neapolitanus as NSOB. As the 

	deterioration mechanisms involved were similar, an acceleration factor 

	with respect to the rate of in situ biodeterioration was determined 

	for laboratory experiment.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Beaumelle2017,

title = {Relationships between metal compartmentalization and biomarkers in earthworms exposed to field-contaminated soils},

author = {Léa Beaumelle and Mickaël Hedde and Franck Vandenbulcke and Isabelle Lamy},

year  = {2017},

date = {2017-01-01},

journal = {Environmental Pollution},

volume = {224},

pages = {185-194},

abstract = {Partitioning tissue metal concentration into subcellular compartments reflecting toxicologically available pools may provide good descriptors of the toxicological effects of metals on organisms. Here we investigated the relationships between internal compartmentalization of Cd, Pb and Zn and biomarker responses in a model soil organism: the earthworm. The aim of this study was to identify metal fractions reflecting the toxic pressure in an endogeic, naturally occurring earthworm species (Aporrectodea caliginosa) exposed to realistic field-contaminated soils. After a 21 days exposure experiment to 31 field-contaminated soils, Cd, Pb and Zn concentrations in earthworms and in three subcellular 

 ractions (cytosol, debris and granules) were quantified. Different biomarkers were measured: the expression of a metallothionein gene (mt), the activity of catalase (CAT) and of glutathione-s-transferase (GST), and the protein, lipid and glycogen reserves. Biomarkers were further combined into an integrated biomarker index (IBR). The subcellular fractionation provided better predictors of biomarkers than the total internal contents hence supporting its use when assessing toxicological bioavailability of metals to earthworms. The most soluble internal pools of metals were not always the best predictors of biomarker responses. metallothionein expression responded to increasing concentrations 

 of Cd in the insoluble fraction (debris granules). Protein and glycogen contents were also mainly related to Cd and Pb in the insoluble fraction. On the other hand, GST activity was better explained by Pb in the cytosolic fraction. CAT activity and lipid contents variations were not related to metal subcellular distribution. The IBR was best explained by both soluble and insoluble fractions of Pb and Cd. This study further extends the scope of mt expression as a robust and specific biomarker in an ecologically representative earthworm species exposed to field-contaminated soils. The genetic lineage of the individuals, assessed by DNA barcoding with cytochrome c oxidase subunit I, did not influence mt expression. (C) 2017 Elsevier Ltd. All rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Chaabene2017b,

title = {The phytochelatin synthase gene in date palm (Phoenix dactylifera L.): Phylogeny, evolution and expression},

author = {Zayneb Chaâbene and Hakim-Imen Rekik and Walid Kriaa and C.-Douglas Grubb and Bassem Khemakhem and Franck Vandenbulcke and Hafedh Mejdoub and Amine Elleuch},

year  = {2017},

date = {2017-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {140},

pages = {7-17},

abstract = {We studied date palm phytochelatin synthase type i (pdpcs1), which catalyzes the cytosolic synthesis of phytochelatins (pcs), a heavy metal binding protein, in plant cells. the gene encoding pdpcs1 (pdpcs) consists of 8 exons and 7 introns and encodes a protein of 528 amino acids. pcs gene history was studied using notung phylogeny. during evolution, gene loss from several lineages was predicted including proteobacteria, bilateria and brassicaceae. in addition, eleven gene duplication events appeared toward interior nodes of the reconciled tree and four gene duplication events appeared toward the external nodes. these latter sequences belong to species with a second copy of pcs suggesting that this gene evolved through subfunctionalization. pdpcs1 gene expression was measured in seedling hypocotyls exposed to cd, cu and cr using quantitative real-time polymerase chain reaction (qpcr). a pdpcs1 overexpression was evidenced in p. dactylifera seedlings exposed to metals suggesting that 1-the pdpcs1 gene is functional, 2-there is an implication of the enzyme in metal detoxification mechanisms. additionally, the structure of pdpcs1 was predicted using its homologue from nostoc (cyanobacterium, nspcs) as a template in discovery studio and pymol software. these analyses allowed us to identify the phytochelatin synthase type i enzyme in date palm (pdpcs1) via recognition of key consensus amino acids involved in the catalytic mechanism, and to propose a hypothetical binding and catalytic site for an additional substrate binding cavity.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Courtois2017,

title = {Sewage sludge, silver nanoparticles and terrestrial environments: state of the art and involved work},

author = {Pauline Courtois and Agnieszka Rorat and Sébastien Lemière and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {International Conference of Environmental Biotechnology, 12th December 2017, Czestochowa (Poland)},

abstract = {The fast increase of the global population, urbanization, industrialization and the improvement of technologies used in wastewater treatment plant have led to a strong increase of the production of sewage sludge. because of the high organic matter and nutrients content, sewage sludge can be applied as a fertilizer, but some contaminants shall be strictly monitored. in europe, legislation focused on: seven metal trace elements (cadmium, chromium, copper, mercury, nickel, lead and zinc) and organic traces compounds (7 polychlobiphenyls and 3 polycyclic aromatic hydrocarbons (fluoranthene, benzo(b)fluoranthene and benzo(a)pyrene)). still, some potentially dangerous contaminants, e.g. silver nanoparticles are not regulated at present. due to their biocidal properties, silver nanoparticles are among the most frequently used nanomaterials (in more of 300 products and production estimated around 500 tons per year). releases in the environment through wastewater treatment plants, linked to the life cycle of products containing nano-silver, can cause a contamination of ecosystem and ecotoxicological and/or health effects [1]. many research aimed on the assessment of the risks and dangers associated with the use of silver, nano-silver and their derivatives on organisms and ecosystems. recent works have shown that the nano-silver 1) decrease the diversity and the quantity of soil microorganisms [2], 2) are accumulated in plant and animal tissues [3,4] and 3) cause biological effects on studied organisms. nevertheless, the understanding of their effects on the terrestrial ecosystem is still incomplete and many questions subsist. this work aims on the effects of the nano-silver toxicity in terrestrial environment, mainly throught its effects on organisms living in close contact with soil, to characterize it and to understand its mechanisms of action on organisms. references [1] anses, 2015. avis de l’agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail. [2] schlich k.et hund-rinke, k., 2015. influence of soil properties on the effect of silver nanomaterials on microbial activity in five soils. environmental pollution. 196, 321–330. [3] colman b.p., arnaout c.l., anciaux s., gunsch c.k., hochella jr m.f., kim b., lowry g.v., mcgill b.m., reinsch b.c., c.j. richardson, unrine j.m., wright j.p., yin l. et bernhardt e.s. 2013. low concentrations of silver nanoparticles in biosolids cause adverse ecosystem responses under realistic field scenario. plos one 8(2): e57189. doi: 10.1371/journal.pone.0057189 [4] shoults-wilson, w.a., reinsch, b.c., tsyusko, o.v., bertsch, p.m., lowry, g.v., unrine, j.m., 2011. effect of silver nanoparticle surface coating on bioaccumulation and reproductive toxicity in earthworms ( eisenia fetida ). nanotoxicology 5, 432–444.},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Jaskulak2017a,

title = {Expression of metallothioneins and phytochelatins in Lupinus luteus L. exposed to heavy metals and nanoparticles},

author = {Marta Jaskulak and Aneta Murtaś and Agnieszka Rorat and Anna Grobelak and Zayneb Chaâbene and Małgorzata Kacprzak and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {SETAC North America 38th Annual Meeting, 12-16 November 2017, Minneapolis},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Murtas2017a,

title = {Expression of metallothionein coding gene in Festuca pratensis H. grown on artificially contaminated soils with silver nanoparticles or ionic silver},

author = {Aneta Murtaś and Marta Jaskulak and Agnieszka Rorat and Anna Grobelak and Zayneb Chaâbene and Małgorzata Kacprzak and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {SETAC North America 38th Annual Meeting, 12-16 November 2017, Minneapolis},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Rorat2017b,

title = {ETNA2 project: Silver ecotoxicology in terrestrial environments},

author = {Agnieszka Rorat and Pauline Courtois and Sébastien Lemière and E Attard and Rémy Guyoneaud and C Levard and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {International Conference of Environmental Biotechnology, 12th December 2017, Czestochowa (Poland)},

abstract = {Due to the increasing incorporation of silver nanoparticles (agnp) in consumer products, silver contamination of sewage sludge may increase and have environmental impacts. indeed sewage sludge are sometimes used in agriculture as fertilizers. in such a context, our project etna2 propose to monitor the silver concentrations in selected sewage sludge coming from southern poland during 2 years and examine their evolution in term of concentrations. in parallel, the assessment of the terrestrial ecotoxicity of silver ions, silver nanoparticles (agnp) and their transformation products (sulphides of silver) will be conducted. therefore, such ecotoxicological assessment will be done using key organisms of soil functioning which are affected by chemical contamination: microorganisms and earthworms. exposures of the ecotoxicologically important test species eisenia fetida were conducted in mesocosms containing agricultural reference soil contaminated by sewage sludge, ag ions, agnp or silver transformation products. these compounds were added to the soil or through the sludge after fermentation. our aim is to address the lack of informations concerning the question of silver in soil ecotoxicology. the impact of ag on soil microflora communities was explored as preliminary experiments evidenced the bactericidal properties of ag in this environmental context. a better understanding of the effects of ag, depending on its form (sulphides from wwtp sludge, ag ions and agnps) will be achieved mainly through genomic and trancriptomic approaches combined with full physico-chemical characterization. analysis will be conducted to make a link between silver evolution/ behavior in sludge and soils and their terrestrial ecotoxicity},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Rorat2017c,

title = {Silver and silver nanoparticles as a threat for terrestrial ecosystems},

author = {Agnieszka Rorat and Pauline Courtois and Sébastien Lemière and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {The XXVI International Symposium “Molecular and Physiological Aspects of Regulatory Processes of the Organism, 16 September 2017, Kraków (Poland)},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Rorat2017d,

title = {Evaluation du vermicompostage appliqué aux boues d'épuration par la surveillance de la qualité du compost et des réponses immunitaires de trois espèces de vers de terre: Eisenia fetida, Eisenia andrei et Dendrobaena veneta},

author = {Agnieszka Rorat and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {Colloque 2017 de la Société Française d'Ecotoxicologie Fondamentale et Appliquée, 29-30 juin 2017, Lille.},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Rorat2017d,

title = {Protective role of metallothionein during regeneration in Eisenia andrei exposed to cadmium},

author = {Agnieszka Rorat and Franck Vandenbulcke and Adrianna Gałuszka and Beata Klimek and Barbara Plytycz},

year  = {2017},

date = {2017-01-01},

journal = {Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology},

volume = {203},

pages = {39-50},

abstract = {Lumbricid earthworms are often exposed to simultaneous action of various environmental stressors like soil contamination, temperature fluctuation or predators' attacks, which may induce extrusion of coelomocytecontaining coelomic fluid or loss of tail segments. if the injuries are not lethal, renewal of the immune-competent cells and soluble components of coelomic fluid and/or the regeneration of tail segments occurs. the aim of our investigations was to test the hypothesis that exposure of adult earthworms eisenia andrei to cadmium-polluted soil at room temperature (rt) and/or low temperature (6 °c) have adverse effects on restoration of experimentally depleted coelomocytes or on regeneration of amputated posterior segments. intact control earthworms and their experimental counterparts subjected to electrostimulation-induced coelomocyte depletion or surgical amputation of posterior segments were maintained either in control soil or in soil spiked with cadmium chloride (500 mg/kg air-dried soil) at rt or 6 °c. four weeks after the beginning of experiments, cadmium accumulation in earthworm bodies was significantly lower at 6 °c than at room temperature. the numbers of restored cells and fluorophore contents were hardly affected by temperature or cadmium. however, cocoon production was reduced by cadmium and completely abolished at 6 °c and regeneration of amputated posterior segments was inhibited in cold but was enhanced by cadmium exposure at rt. independently on the temperature, the 4-week cadmium exposure of adult earthworms was connected with significantly upregulated expression of cd-metallothionein (but not of catalase, lysenin and phytochelatin) in coelomocytes},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Rorat2017a,

title = {Vermiremediation of polycyclic aromatic hydrocarbons and heavy metals in sewage sludge composting process},

author = {Agnieszka Rorat and Dariusz Wloka and Anna Grobelak and Anna Grosser and Aleksandra Sosnecka and Marcin Milczarek and Paulina Jelonek and Franck Vandenbulcke and Malgorzata Kacprzak},

year  = {2017},

date = {2017-01-01},

journal = {Journal of Environmental Management},

volume = {187},

pages = {347-353},

abstract = {The main objective of this work was to study the dynamics of the degradation of polycyclic aromatic hydrocarbons (pahs) during sewage sludge vermicomposting. this eco-biotechnology employing earthworms as natural bioreactors for decomposing of organic matter may be used for vermiremediation of particular pollutants present in various organic matter sources. in this experiment, sewage sludge was mixed with bulking agents and precomposted. afterward, adult eisenia andrei specimens were introduced into the process. total heavy metal and pahs concentration were measured in composts and earthworms before and after the process. while heavy metal concentrations fluctuated mildly in the substratum, several metals clearly accumulated in the earthworms' bodies. body accumulation factors could be ranked as follows (cd > cu > zn > ni > cr > pb). interestingly, addition of earthworms into the process has led to the high percentage of pahs removal and some of the 16 priority pahs analyzed in this study have been accumulated in earthworms' bodies. applied conditions did not affect worms' viability but they almost completely inhibited their reproduction.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Suleiman2017,

title = {Determination of the performance of vermicomposting process applied to sewage sludge by monitoring of the compost quality and immune responses in three earthworm species: Eisenia fetida, Eisenia andrei and Dendrobaena veneta},

author = {Hanine Suleiman and Agnieszka Rorat and Anna Grobelak and Anna Grosser and Marcin Milczarek and Barbara Płytycz and Małgorzata Kacprzak and Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

journal = {Bioresource Technology},

volume = {241},

pages = {103-112},

abstract = {The aim of this study was to assess the effectiveness of vermicomposting process applied on three different sewage sludge (precomposted with grass clippings, sawdust and municipal solid wastes) using three different earthworm species. selected immune parameters, namely biomarkers of stress and metal body burdens, have been used to biomonitor the vermicomposting process and to assess the impact of contaminants on earthworm’s physiology. biotic and abiotic parameters were also used in order to monitor the process and the quality of the final product. dendrobaena veneta exhibited much lower resistance in all experimental conditions, as the bodyweight and the total number of circulating immune cells decreased in the most contaminated conditions. all earthworm species accumulated heavy metals as follows cd > co > cu > zn > ni > pb > cr: eisenia sp. worms exhibited the highest ability to accumulate several heavy metals. vermicompost obtained after 45 days was acceptable according to agronomic parameters and to compost quality norms in france and poland},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Vandenbulcke2017,

title = {Silver nanoparticles in sewage sludge- a new threat?},

author = {Franck Vandenbulcke},

year  = {2017},

date = {2017-01-01},

booktitle = {International Conference of Environmental Biotechnology. Ecole Polytechnique de Czestochowa, 12-13 Décember 2017 (Poland)},

note = {INV},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Beaumelle2016,

title = {A structural equation model of soil metal bioavailability to earthworms: confronting causal theory and observations using a laboratory exposure to field-contaminated soils},

author = {Léa Beaumelle and Denis Vile and Isabelle Lamy and Franck Vandenbulcke and Frédéric Gimbert and Mickaël Hedde},

year  = {2016},

date = {2016-01-01},

journal = {Science of the Total Environment},

volume = {569},

pages = {961-972},

abstract = {Structural equation models (sem) are increasingly used in ecology as multivariate analysis that can represent theoretical variables and address complex sets of hypotheses. here we demonstrate the interest of sem in ecotoxicology, more precisely to test the three-step concept of metal bioavailability to earthworms. the sem modeled the three-step causal chain between environmental availability, environmental bioavailability and toxicological bioavailability. in the model, each step is an unmeasured (latent) variable reflected by several observed variables. in an exposure experiment designed specifically to test this sem for cd, pb and zn, aporrectodea caliginosa was exposed to 31 agricultural field-contaminated soils. chemical and biological measurements used included cacl2-extractable metal concentrations in soils, free ion concentration in soil solution as predicted by a geochemical model, dissolved metal concentration as predicted by a semi-mechanistic model, internal metal concentrations in total earthworms and in subcellular fractions, and several biomarkers. the observations verified the causal definition of cd and pb bioavailability in the sem, but not for zn. several indicators consistently reflected the hypothetical causal definition and could thus be pertinent measurements of cd and pb bioavailability to earthworm in field-contaminated soils. sem highlights that the metals present in the soil solution and easily extractable are not the main source of available metals for earthworms. this study further highlights sem as a powerful tool that can handle natural ecosystem complexity, thus participating to the paradigm change in ecotoxicology from a bottom-up to a top-down approach. (c) 2016 elsevier b.v. all rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Bernard2016,

title = {Antioxidant defense gene analysis in Brassica oleracea and Trifolium repens exposed to Cd and/or Pb},

author = {Fabien Bernard and Sylvain Dumez and Franck Brulle and Sébastien Lemière and Anne Platel and Fabrice Nesslany and Damien Cuny and Annabelle Deram and Franck Vandenbulcke},

year  = {2016},

date = {2016-01-01},

journal = {Environmental Science and Pollution Research},

volume = {23},

number = {4},

pages = {3136-3151},

abstract = {This study focused on the expression analysis of antioxidant defense genes in brassica oleracea and in trifolium repens. plants were exposed for 3, 10, and 56 days in microcosms to a field-collected suburban soil spiked by low concentrations of cadmium and/or lead. in both species, metal accumulations and expression levels of genes encoding proteins involved and/or related to antioxidant defense systems (glutathione transferases, peroxidases, catalases, metallothioneins) were quantified in leaves in order to better understand the detoxification processes involved following exposure to metals. it appeared that strongest gene expression variations in t. repens were observed when plants are exposed to cd (metallothionein and ascorbate peroxidase upregulations) whereas strongest variations in b. oleracea were observed in case of cd/pb co-exposures (metallothionein, glutathione transferase, and peroxidase upregulations). results also suggest that there is a benefit to use complementary species in order to better apprehend the biological effects in ecotoxicology},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Rorat2016,

title = {Interactions between sewage sludge-amended soil and earthworms—comparison between Eisenia fetida and Eisenia andrei composting species},

author = {Agnieszka Rorat and Hanine Suleiman and Anna Grobelak and Anna Grosser and Malgorzata Kacprzak and Barbara Plytycz and Franck Vandenbulcke},

year  = {2016},

date = {2016-01-01},

journal = {Environmental Science and Pollution Research},

volume = {23},

number = {4},

pages = {3026-3035},

abstract = {Vermicomposting is an eco-friendly technology, where earthworms are introduced in the waste, inter alia sewage sludge, to cooperate with microorganisms and enhance decomposition of organic matter. the main aims of the present study was to determine the influence of two different earthworm species, eisenia fetida and eisenia andrei, on the changes of selected metallic trace elements content in substratum during vermicomposting process using three different sewage sludge mainly differentiated by their metal contents. final vermicompost has shown a slight reduction in cd, cu, ni, and pb, while the zn concentration tends to increase. accumulation of particular heavy metals in earthworms’ bodies was assessed. both species revealed high tendency to accumulate cd and zn, but not cu, ni, and pb, but e. andrei has higher capabilities to accumulate some metals. riboflavin content, which content varies depending on metal pollution in several earthworms species, was measured supravitaly in extruded coelomocytes. riboflavin content decreased slightly during the first 6 weeks of exposure and subsequently restored till the end of the 9-week experiment. selected agronomic parameters have also been measured in the final product (vermicompost) to assess the influence of earthworms on substratum},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Souleman2016,

title = {Isolation and characterization of eight polymorphic microsatellites markers for the earthworm Lumbricus terrestris},

author = {Dima Souleman and Fabien Grumiaux and Hélène Frérot and Franck Vandenbulcke and Maxime Pauwels},

year  = {2016},

date = {2016-01-01},

journal = {European Journal of Soil Biology},

volume = {74},

pages = {76-80},

abstract = {The earthworm lumbricus terrestris, which is an ecologically important sentinel species for soils widely distributed throughout northern europe, has never been involved in deep population genetic studies. in order to promote future studies, we report here the isolation and characterization of 8 new polymorphic microsatellite loci isolated from sequencing of a microsatellite-enriched genomic library. microsatellite markers were tested on 192 field collected individuals. allelic richness per locus varied from 4.921 to 24.876, with a mean value of 6.125 alleles per locus. the observed heterozygosity (ho) ranged from 0.132 to 0.839, whereas the expected heterozygosity (he) ranged from 0.407 to 0.926. developed markers should be helpful for accurate estimation of population structure, dispersal and gene flow among populations},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Vandenbulcke2016,

title = {Nanoparticles in waste: New threat?},

author = {Franck Vandenbulcke},

year  = {2016},

date = {2016-01-01},

booktitle = {ASSM (Advances in Sustainable Sewage sludge Management) 2016 International Conference, 18-22 September 2016, Cracovie (Pologne)},

note = {INV},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Lanier2016,

title = {Combined effect of Cd and Pb spiked field soils on bioaccumulation, DNA damage, and peroxidase activities in Trifolium repens},

author = {Caroline Lanier and Fabien Bernard and Sylvain Dumez and Julie Leclercq-Dransart and Sébastien Lemière and Franck Vandenbulcke and Fabrice Nesslany and Anne Platel and I Devred and Damien Cuny and Annabelle Deram},

year  = {2016},

date = {2016-01-01},

journal = {Environmental Science and Pollution Research},

volume = {23},

number = {2},

pages = {1755-1767},

abstract = {The present study was designed to investigate the combined effects of cd and pb on accumulation and genotoxic potential in white clover (trifolium repens). for this purpose, t. repens was exposed to contaminated soils (2.5–20 mg kg−1 cadmium (cd), 250–2000 mg kg−1 lead (pb) and a mixture of these two heavy metals) for 3, 10 and 56 days. the resulting bioaccumulation of cd and pb, dna damage (comet assay) and peroxidase activities (apox and gpox) were determined. the exposure time is a determinant factor in experiments designed to measure the influence of heavy metal contamination. the accumulation of cd or pb resulting from exposure to the two-metal mixture does not appear to depend significantly on whether the white clover is exposed to soil containing one heavy metal or both. however, when t. repens is exposed to a cd/pb mixture, the percentage of dna damage is lower than when the plant is exposed to monometallic cd. dna damage is close to that observed in the case of monometallic pb exposure. peroxidase activity cannot be associated with dna damage under these experimental conditions},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Bernard2015,

title = {Antioxidant responses of Annelids, Brassicaceae and Fabaceae to pollutants: A review},

author = {Fabien Bernard and Franck Brulle and Sylvain Dumez and Sébastien Lemière and Anne Platel and Fabrice Nesslany and Damien Cuny and Annabelle Deram and Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {114},

pages = {273-303},

abstract = {Pollutants, such as Metal Trace Elements (MTEs) and organic compounds 

	(polycyclic aromatic hydrocarbons, pesticides), can impact DNA structure 

	of living organisms and thus generate damage. For instance, cadmium 

	is a well-known genotoxic and mechanisms explaining its clastogenicity 

	are mainly indirect: inhibition of DNA repair mechanisms and/or induction 

	of Reactive Oxygen Species (ROS). Animal or vegetal cells use antioxidant 

	defense systems to protect themselves against ROS produced during 

	oxidative stress. Because tolerance of organisms depends, at least 

	partially, on their ability to cope with ROS, the mechanisms of production 

	and management of ROS were investigated a lot in Ecotoxicology as 

	markers of biotic and abiotic stress. This was mainly done through 

	the measurement of enzyme activities The present Review focuses on 

	3 test species living in close contact with soil that are often used 

	in soil ecotoxicology: the worm Eisenia fetida, and two plant species, 

	Trifolium repens (white clover) and Brassica oleracea (cabbage). 

	E. fetida is a soil-dwelling organism commonly used for biomonitoring. 

	T. repens is a symbiotic plant species which forms root nodule with 

	soil bacteria, while B. oleracea is a non-symbiotic plant. In literature, 

	some oxidative stress enzyme activities have already been measured 

	in those species but such analyses do not allow distinction between 

	individual enzyme involvements in oxidative stress. Gene expression 

	studies would allow this distinction at the transcriptomic level. 

 

	A literature review and a data search in molecular database were carried 

	out on the basis of keywords in Scopus, in PubMed and in Genbank™ 

	for each species. Molecular data regarding E. fetida were already 

	available in databases, but a lack of data regarding oxidative stress 

	related genes was observed for T. repens and B. oleracea. By exploiting 

	the conservation observed between species and using molecular biology 

	techniques, we partially cloned missing candidates involved in oxidative 

	stress and in metal detoxification in E. fetida, T. repens and B. 

	oleracea.},

note = {ACLS},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Bernard2015a,

title = {Gene expression analysis of antioxidant responses in Annelids, Brassicaceae and Fabaceae exposed to cadmium and/or lead contaminated soils},

author = {Fabien Bernard and Franck Brulle and Sylvain Dumez and Sébastien Lemière and Anne Platel and Fabrice Nesslany and Damien Cuny and Annabelle Deram and Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

booktitle = {SETAC, 3-7 May 2015, Barcelone (Espagne)},

abstract = {Pollutants, such as Metal Trace Elements (MTEs) and organic compounds 

	(polycyclic aromatic hydrocarbons, pesticides), can impact DNA structure 

	of living organisms and thus generate DNA damage. Animal or vegetal 

	cells use antioxidant defense systems to protect themselves against 

	Reactive Oxygen Species (ROS) produced during oxidative stress. The 

	mechanisms of production and management of ROS were investigated 

	a lot in ecotoxicology as unspecific markers. We performed a literature 

	survey of the biochemical responses (enzyme activities) and expression 

	variations of genes encoding proteins involved and/or related to 

	antioxidant response in three species that are often used in ecotoxicology 

	and that live in close contact with soil: the earthworm, Eisenia 

	fetida, the white clover, Trifolium repens and the cabbage, Brassica 

	oleracea. Literature survey and data search in molecular database 

	were carried out on the basis of keywords in Scopus, in PubMed and 

	in Genbank™ for each species (and in related species). In literature, 

	some antioxidant enzyme activities have already been measured in 

	those species but such analysis do not allow distinction of individual 

	enzyme involvements in oxidative stress. Gene expression studies 

	would allow this distinction at the transcriptomic level. By exploiting 

	the conservation observed between species and using molecular biology 

	techniques, we partially cloned and measured the gene expression 

	of many candidates involved and/or related to antioxidant responses 

	in E. fetida, T. repens and B. oleracea. Therefore, analysis of the 

	gene expression level of most effectors involved and/or related to 

	antioxidant responses has been performed in the 3 species exposed 

	in vivo (3, 10 and 56 days) in a well-characterised sub-urban reference 

	soil spiked with cadmium and/or lead at environmental concentrations. 

	The use of gene expression analysis (in combination with metal accumulation 

	analysis) has shown the interest of studying the effects of metal 

	stress in several species. A major interest of this work lies in 

	the measurement of most genes involved and/or related to antioxidant 

	response at the same time and in several species. Indeed, as responses 

	to oxidative stress may be different between species for the same 

	protein family, one may keep in mind that model species should be 

	used in a complementary manner in order to better understand the 

	effects of xenobiotics on exposed organisms.},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Homa2015,

title = {Dermal exposure of Eisenia andrei earthworms: Effects of heavy metals on metallothionein and phytochelatin synthase gene expressions in coelomocytes},

author = {Joanna Homa and Agnieszka Rorat and Jerzy Kruk and Claude Cocquerelle and Barbara Plytycz and Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

journal = {Environmental Toxicology and Chemistry},

volume = {34},

number = {6},

pages = {1397-1404},

abstract = {Parameters such as total number of coelomocytes, riboflavin content in coelomocytes, expression of genes implied in metal homeostasis, and detoxification mechanisms can be used as biomarkers to assess the impact of metals on annelids. defense biomarkers (detoxification gene expressions and coelomocyte parameters) were investigated in the ecotoxicologically important species eisenia andrei following in vivo exposure to 5 different metals (zinc, copper, nickel, lead, and cadmium) at known concentrations. coelomocyte numbers and riboflavin content were not affected by metallic exposure, but metal-specific gene expression variations were evidenced},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Lemiere2015,

title = {Is Eisenia fetida a suitable species to monitor environmental soil genotoxicants},

author = {Sébastien Lemière and Annabelle Deram and Fabien Bernard and Alain Leprêtre and Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

booktitle = {SETAC, 3-7 May 2015, Barcelone (Espagne)},

abstract = {As well as for air and waters, not only chemical analyses have to be taken into account to monitor anthropic contamination of soils. biological responses of exposed organisms should be considered too and particularly for environmental genotoxicants as pahs or metals. it is well-known that studies of soil contaminations are complex. for example, the environmental availability, environmental and toxicological bioavailabilities of present metal contaminants, influenced by soil factors, ageing phenomena, organic matter..., their concomitant presences, and often at low concentrations and then their potential toxic interactions have to be considered. that’s why methods of classical genetic toxicology (indirect testing, unrealistic exposure...) did not seem fully satisfactory. several years ago, we developed an environmental genotoxicity marker with eisenia fetida,the comet assay. it is a technique allowing the evaluation of dna damage (single and double strand breaks, alkali-labile sites) in a cellular population and it was developed on cœlomocytes of this biological model largely used in soil ecotoxicology. in this poster presentation, we will present obtained results in different contexts with this environmental genotoxicty marker: exposures to field-contaminated soils (1), to spiked soils with a mixture of metals (2) and to soils after a remediation treatment (3). in the first work (1), in vivo short-term exposures of e. fetida were conducted using soils sampled in two different areas in the north of france: from the vicinity of a former smelter and from a suburb zone close to a battery plant still in activity. in the second (2), in order to better understand metal interactions, in vivo short to mid-term exposures (3, 10 and 56 days) were performed using an urban-surrounding soil spiked with cadmium or/and lead at concentrations found in french contaminated soils. in the last work (3), in vivo short-term exposures of e. fetida were conducted using a contaminated soil sampled in the field and a reference soil contaminated or not by a mixture of lead, zinc and cadmium. in both soils were added (or not) remediation agents (biochars from two origins) at two concentrations. in all cases, obtained genotoxicity results after these exposures will be detailed. we will try to discuss the interests and limits of our environmental genotoxicity marker and to conclude if it is useful and relevant to monitor anthropic contaminated soils.},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pauget2015,

title = {Soil bioindicators: how soil properties influence their responses and how to select them in function of the site issues?},

author = {Benjamin Pauget and Laurence Rougé and Antonio Bispo and Cécile Grand and Thierry Béguiristain and Jean-Jacques Bessoule and Josselin Bodilis and Rémi Chaussod and Nathalie Cheviron and Michaël Coeurdassier and Jérôme Cortet and Steven Criquet and Samuel Dequiedt and Olivier Faure and Christophe Gangneux and Isabelle Gattin and Marina Le-Guédard and Adnane Hitmi and Nicolas Laurent and Marc Legras and Sylvie Nélieu and Nuria Ruiz and Salima Taibi and Franck Vandenbulcke and Annette de-Vaufleury and Cécile Villenave and Guénola Pérès},

year  = {2015},

date = {2015-01-01},

booktitle = {SETAC, 3-7 May 2015, Barcelone (Espagne)},

abstract = {Context - despite no directive exists for soil protection, this key component of ecosystem needs to be protected. to fill the lack of tools to monitor the soil threats and to assess the impact of soil management, biological indicators have been developed in the french program bioindicators 2 (ademe). on the 47 plots of 13 sites (agricultural, industrial and forest), the influence of soil characteristics and soil use on the response of 80 biological parameters (fauna, flora and microorganisms) have been assessed by using linear multivariate regressions. results - the soil texture and the organic carbon rate has been identified as the main soil characteristic influencing the biological responses, even if each bioindicator is influenced by its own pool of soil parameters. it underlines the necessity to use battery of bioindicators for an integrative assessment of soil quality. an influence of soil contaminants (organic or metallic) have been highlighted for 86% of the bioindicators showing the necessity to consider their bioavailability for suitable soil management. to disseminate the use of bioindicators, a web interface has been implemented (http://ecobiosoil.univ-rennes1.fr/ademe-bioindicateur/). it presents all the biological methods developed in the program and allows consultation of the variation range of each bioindicator considering site contexts and soil physico-chemical characteristics of soils thus leading to provide a first benchmark. it also provides tools to the end-users to select the bioindicator(s) which best fit(s) with their own site problematic (agricultural practices, bioavailability of contaminants, soil monitoring...) and to communicate (technical sheets...). conclusion – for the first time, the soil properties influence on the response of 80 bioindicators was characterized under similar environmental conditions. as each bioindicator is influenced by a specific pool of soil parameter, chemical measures cannot be used as a surrogate of biological measures. this program has provided usable tools for a biologically-based site management},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Peres2015,

title = {Which bioindicators are suitable for soil quality monitoring and risk assessment? From relevance study to transfer tool development},

author = {Guénola Pérès and Benjamin Pauget and Annette De-Vaufleury and Michaël Coeurdassier and Marina Le-Guédard and Jean-Jacques Bessoule and Samuel Dequiedt and Rémi Chaussod and Lionel Ranjard and Daniel Cluzeau and Muriel Guernion and Laurence Rougé and Mickaël Hedde and Nathalie Cheviron and Jeanne-Chantal Dur and Sylvie Nélieu and Christian Mougin and Isabelle Gattin and Christophe Gangneux and Nicolas Laurent and Marc Legras and Karine Laval and Patrice Lepelletier and Salima Taibi and Cécile Villenave and Olivier Faure and Jennifer Hellal and Jérôme Cortet and Thierry Béguiristain and Corinne Leyval and Josselin Bodilis and Steven Criquet and Adnane Hitmi and Nuria Ruiz and Franck Vandenbulcke and Cécile Grand and Laurence Galsomies and Antonio Bispo},

year  = {2015},

date = {2015-01-01},

booktitle = {SETAC, 3-7 May 2015, Barcelone (Espagne)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Suleiman2015,

title = {Comparing different strategies of vermicomposting},

author = {Hanine Suleiman and Agnieszka Rorat and Barbara Plytycz and Malgorzata Kacprzak and Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

booktitle = {Journée des Doctorants IRePSE, 08 juillet 2015, CERLA - Cité Scientifique - Université Lille 1, Villeneuve d'Ascq},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke2015,

title = {Questionnements sur les aspects environnementaux du nano-argent : une nanoparticule d’origine anthropique déjà présente dans l’environnement?},

author = {Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

booktitle = {Forum Nanoresp. Les usages du nanoargent, 6 mai 2015, Paris},

abstract = {Les matériaux à base d’argent à l’état nanoparticulaire, ou nanoargent, sont utilisés pour leurs propriétés antimicrobiennes dans des textiles, des déodorants, des désinfectants, des cosmétiques, des emballages alimentaires, des lessives, dans des revêtements d’électroménager, des composants électroniques, des pansements servant à prévenir les infections après des brûlures, etc. que sait-on aujourd’hui des propriétés et des effets du nanoargent ? existe-t-il des procédés pour l’intégrer dans des matériaux de façon stable et durable ? peut-on l’associer à d’autres nanomatériaux ? dans quelles conditions peut-il se révéler toxique ? par exemple, la concentration d’argent dans les boues d’épuration présente-t-elle un risque potentiel pour l’environnement via l’épandage de ces boues sur les terres agricoles ? en bref, à qui profite le nanoargent ?},

note = {INV},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke2015a,

title = {Valorisation des boues des stations d’épuration sur les sols agricoles, focus sur une contamination émergente},

author = {Franck Vandenbulcke},

year  = {2015},

date = {2015-01-01},

booktitle = {ANSES, 22 avril 2015, Paris},

note = {INV},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke2015b,

title = {Ecotoxicologie des nano-objets : le nano-argent, une nanoparticule d’origine anthropique déjà rejetée dans l’environnement?},

author = {Franck Vandenbulcke and Sébastien Lemière},

year  = {2015},

date = {2015-01-01},

booktitle = {Journée thématique "nano-poudres, nano-materiaux, nano-objets: produits du futur, de la molecule au consommateur", Ecole Centrale de Lille, 29 janvier 2015, Lille},

note = {INV},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Brulle2014,

title = {Identification of suitable qPCR reference genes in leaves of Brassica oleracea under abiotic stresses},

author = {Franck Brulle and Fabien Bernard and Franck Vandenbulcke and Damien Cuny and Sylvain Dumez},

year  = {2014},

date = {2014-01-01},

journal = {Ecotoxicology},

volume = {23},

number = {3},

pages = {459-471},

abstract = {Real-time quantitative pcr is nowadays a standard method to study gene expression variations in various samples and experimental conditions. however, to interpret results accurately, data normalization with appropriate reference genes appears to be crucial. the present study describes the identification and the validation of suitable reference genes in brassica oleracea leaves. expression stability of eight candidates was tested following drought and cold abiotic stresses by using three different softwares (bestkeeper, normfinder and genorm). four genes (bolc.tub6, bolc.sand1, bolc.ubq2 and bolc.tbp1) emerged as the most stable across the tested conditions. further gene expression analysis of a drought- and a cold-responsive gene (bolc.dreb2a and bolc.elip, respectively), confirmed the stability and the reliability of the identified reference genes when used for normalization in the leaves of b. oleracea. these four genes were finally tested upon a benzene exposure and all appeared to be useful reference genes along this toxicological condition. these results provide a good starting point for future studies involving gene expression measurement on leaves of b. oleracea exposed to environmental modifications},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Deram2014,

title = {Complémentarité des modèles végétaux et animaux pour le diagnostic de la fraction génotoxique de sols contaminés},

author = {Annabelle Deram and Sébastien Lemière and Fabien Bernard and Franck Marot and Sylvain Dumez and Anne Platel and Fabrice Nesslany and Franck Vandenbulcke and Damien Cuny},

year  = {2014},

date = {2014-01-01},

booktitle = {Colloque des 3èmes Rencontres nationales de la recherche sur les sites et sols pollués, 18-19 novembre 2014, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pauget2014,

title = {Soil bioindicators: how soil properties influence their responses and to select them in function of the site issues?},

author = {Benjamin Pauget and Laurence Rougé and Antonio Bispo and Cécile Grand and Thierry Béguiristain and Jean-Jacques Bessoule and Josselin Bodilis and Rémi Chaussod and Nathalie Cheviron and Michaël Coeurdassier and Jérôme Cortet and Steven Criquet and Samuel Dequiedt and Olivier Faure and Christophe Gangneux and Isabelle Gattin and Marina Le-Guédard and Adnane Hitmi and Nicolas Laurent and Marc Legras and Sylvie Nélieu and Nuria Ruiz and Salima Taïbi and Franck Vandenbulcke and Annette De-Vaufleury and Cécile Villenave and Guénola Pérès},

year  = {2014},

date = {2014-01-01},

booktitle = {First Global Soil Biodiversity Conference - Assessing soil biodiversity and its role for ecosystem services, 2-5 décembre 2014, Dijon},

abstract = {Introduction despite no european directive exists for soil protection, this key component of ecosystem needs to be protected. to fill the lack of tools to monitor the soil threats and to assess the impact of soil management, biological indicators have been developed in the french program bioindicators 2 (ademe) and a website allowing selection of relevant bioindicators depending on the question asked achieved. on the 47 plots of 13 sites (agricultural, industrial and forest), the influence of soil characteristics and soil use on the response of 80 biological parameters (fauna, flora and microorganisms) have been assessed by using linear multivariate regressions.},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pauget2014a,

title = {Les indicateurs de l'état biologique des sols: Guide de sélection selon le type d’application et/ou la problématique},

author = {Benjamin Pauget and Laurence Rougé and Guénola Pérès and Antonio Bispo and Cécile Grand and Thierry Béguiristain and Jean-Jacques Bessoule and Josselin Bodilis and Rémi Chaussod and Nathalie Cheviron and Michaël Coeurdassier and Jérôme Cortet and Stéven Criquet and Samuel Dequiedt and Olivier Faure and Christophe Gangneux and Isabelle Gattin and Marina Le-Guédard and Adnane Hitmi and Nicolas Laurent and Marc Legras and Sylvie Nélieu and Nuria Ruiz and Franck Vandenbulcke and Cécile Villenave and Annette De-Vaufleury},

year  = {2014},

date = {2014-01-01},

booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris},

abstract = {Pour surveiller, caractériser et évaluer les menaces pesant sur les sols et évaluer l’impact des méthodes de gestion et de protection de cette ressource essentielle, des outils de bioindication basés sur les propriétés biologiques des sols ont été mis en oeuvre simultanément dans le cadre du programme bioindicateurs 2 (ademe). l'influence des paramètres des sols et de ses contaminants sur les réponses de 80 paramètres biologiques (faune, flore et microbiens) a été évaluée sur 47 modalités de 13 sites (agricoles, forestiers et sites contaminés). l'étude a permis d'identifier les principales caractéristiques physico-chimiques ux paramètres de texture des sols influençant la réponse des bio indicateurs et montre que ces paramètres sont variables selon le bioindicateur considéré. une forte influence des contaminants, qu'ils soient organiques ou métalliques est également observée, ce qui souligne la nécessité de prendre en compte leur biodisponibilité lors de l'établissement la réalsiation de plan de gestiondes études de risque. pour faciliter l'utilisation des bioindicateurs, une interface web basée sur des critères de sélection d’outils choisis avec leurs utilisateurs potentiels a été mise en place. cette interface présente l’ensemble des méthodes outils biologiques appliquées dans le programme bioindicateurs 2 et permet (i) de consulter les gammes de variation des bioindicateurs dans les différents contextes du programme bioindicateurs 2 à la base de ce 1er référentiel d'interprétation des bioindicateurs et (ii) de sélectionner le bioindicateur correspondant le mieux à sa problématique (impact d’une méthode de dépollution par ex.) sur la base de critères scientifiques, et techniques et économiques.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Pelosi2014,

title = {Pesticides and earthworms. A review},

author = {Céline Pelosi and Sébastien Barot and Yvan Capowiez and Mickaël Hedde and Franck Vandenbulcke},

year  = {2014},

date = {2014-01-01},

journal = {Agronomy for Sustainable Development},

volume = {34},

number = {1},

pages = {199-228},

abstract = {Earthworms provide key soil functions that favour many positive ecosystem services. these services are important for agroecosystem sustainability but can be degraded by intensive cultural practices such as use of pesticides. many literature reports have investigated the effect of pesticides on earthworms. here, we review those reports to assess the relevance of the indicators of earthworm response to pesticides, to assess their sensitivity to pesticides, and to highlight the remaining knowledge gaps. we focus on european earthworm species and products authorised in europe, excluding natural compounds and metals. we consider different organisation levels: the infra-individual level (gene expression and physiology), the individual and population levels (life-history traits, population density and behaviour) and the community level: community biomass and density. our analysis shows that earthworms are impacted by pesticides at all organisation levels. for example, pesticides disrupt enzymatic activities, increase individual mortality, decrease fecundity and growth, change individual behaviour such as feeding rate and decrease the overall community biomass and density. insecticides and fungicides are the most toxic pesticides impacting survival and reproduction, respectively},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Peres2014,

title = {Quels bioindicateurs et biomarqueurs utiliser pour la surveillance de la qualité des sols et l’évaluation des risques environnementaux?},

author = {Guénola Pérès and Benjamin Pauget and Thierry Béguiristain and Jean-Jacques Bessoule and Josselin Bodilis and Rémi Chaussod and Daniel Cluzeau and Nathalie Cheviron and Michaël Coeurdassier and Jérôme Cortet and Stéven Criquet and Samuel Dequiedt and Annette De-Vaufleury and Jeanne-Chantal Dur and Olivier Faure and Christophe Gangneux and Isabelle Gattin and Muriel Guernion and Jennifer Hellal and Mickaël Hedde and Adnane Hitmi and Nicolas Laurent and Marc Legras and Marina Le-Guédard and Patrice Lepelletier and Christian Mougin and Sylvie Nélieu and Stéphane Ponton and Lionel Ranjard and Laurence Rougé and Nuria Ruiz and Salima Taibi and Franck Vandenbulcke and Cécile Villenave and Laurence Galsomies and Cécile Grand and Antonio Bispo},

year  = {2014},

date = {2014-01-01},

booktitle = {12ème Journées d'Etude des Sols, 30 juin-4 juillet 2014, Le Bourget du Lac},

abstract = {Le sol est une ressource essentielle pour les sociétés humaines et les écosystèmes qu’il convient de protéger. mettre en place et suivre des actions de protection, de réhabilitation et de meilleures gestions des sols sur le long terme, nécessite de disposer d’indicateurs capables de renseigner sur la dégradation et/ou la restauration des propriétés et des fonctions des sols. les indicateurs classiquement utilisés reposent sur des propriétés physiques et chimiques des sols alors que les paramètres biologiques, bien que en répondant de manière intégrative aux stress environnementaux permettent de renseignant sur l’état global des sols, sont peu intégrés dans la description de la qualité des sols. face à ce constat d’un manque d’indicateurs biologiques (bioindicateurs) l’ademe a mis en place en 2004 un programme national de recherche qui visait à i) développer de nouvelles méthodes de mesure de la diversité et des fonctions des organismes du sol, les valider et les normaliser dans différents types de sol et d’utilisation, ii) démontrer la sensibilité et la complémentarité des bioindicateurs pour caractériser différentes perturbations (ex : contamination, changement d’usage et de pratiques agricoles), iii) identifier des bioindicateurs pertinents (tant en terme de sensibilité qu’en termes de fonctionnalité, transférabilité) répondant aux besoins et attentes spécifiques des gestionnaires de sols agricoles, forestiers et contaminés (surveillance de la qualité des sols, évaluation des risques environnementaux, évaluation du potentiel de requalification d’un sol ..). grâce à plus de 10 années de travaux, séquencées en deux étapes et impliquant 22 équipes de recherche, le programme a permis non seulement d’acquérir une meilleure compréhension du fonctionnement biologique des sols et d’initier un premier référentiel national, mais également de définir des outils (batteries d’indicateurs, indices agrégés) adaptés aux différentes problématiques environnementales que sont i) l’évaluation des impacts des pratiques agricoles telles que le travail du sol, la gestion des rotations, les apports de déchets ou de pesticides, ii) l’évaluation des transferts des contaminants d’origine industrielle vers la chaîne trophique terrestre et leurs effets, iii) l’évaluation de l’état biologique d’un sol contaminé délaissé afin de le requalifier pour un usage futur, et iv) la biosurveillance des sols sur des sites à grandes échelles. ces principaux acquis seront présentés et discutés en termes de qualité de la réponse biologique, d’accessibilité et de transférabilité des outils biologiques vers les utilisateurs potentiels (http://ecobiosoil.univ-rennes1.fr/ademe-bioindicateur/). le couplage de ces résultats avec d’autres travaux en cours permettra de proposer des batteries optimales d’indicateurs biologiques adaptées à chacune des problématiques listées ci-dessus.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Peres2014a,

title = {Which bioindicators are suitable for soil quality monitoring and risk assessment ? From relevance study to transfer tool development},

author = {Guénola Pérès and Benjamin Pauget and Annette De-Vaufleury and Michaël Coeurdassier and Marina Le-Guédard and Jean-Jacques Bessoule and Samuel Dequiedt and Rémi Chaussod and Lionel Ranjard and Daniel Cluzeau and Muriel Guernion and Laurence Rougé and Mickaël Hedde and Nathalie Cheviron and Jeanne-Chantal Dur and Sylvie Nélieu and Christian Mougin and Isabelle Gattin and Christophe Gangneux and Nicolas Laurent and Marc Legras and Karine Laval and Patrice Lepelletier and Salima Taibi and Cécile Villenave and Olivier Faure and Jennifer Hellal and Jérôme Cortet and Thierry Béguiristain and Corinne Leyval and Josselin Bodilis and Steven Criquet and Adnane Hitmi and Nuria Ruiz and Franck Vandenbulcke and Cécile Grand and Laurence Galsomies and Antonio Bispo},

year  = {2014},

date = {2014-01-01},

booktitle = {First Global Soil Biodiversity Conference - Assessing soil biodiversity and its role for ecosystem services, 2-5 décembre 2014, Dijon},

abstract = {Introduction- considering the few number of bioindicators available for soil risk assessment and soil characterization, a national research programme has been set up in france by the french agency for environment and energy management (ademe) to develop indicators able to judge about soil degradation and/or remediation of its properties and functions. objectives of this “bioindicator programme” are to (i) develop and validate methods for measuring soil biodiversity and soil functions, (ii) test the sensibility and complementarity of the bioindicators for the characterization of different perturbations (contamination, modification of soil uses), iii) identify relevant bioindicators or endpoints for different purposes (ecological risk assessment, monitoring of soil quality..), iv) transfer the results to stakeholders (policy makers, farmers, research board) by providing suitable tools. methods- 22 research teams are involved. 47 bioindicators are tested (microorganisms, fauna, flora) in a large panel of situations (47 situations including agricultural, industrial and forest sites) and sampled at the same moment by applying standardised sampling protocols. a common database allows the management of the high number of data (200.000). results- bioindicator programme initiates a first national benchmark i.e. baselines values for the different biological groups. it provides relevant tools (battery of bioindicators, biological index) adapted to different environmental targets such as i) evaluation of the impact of agricultural practices (crop rotations, reduced tillage, organic and pesticides management), ii) evaluation of the bioavailability of industrial contaminants, iii) evaluation of the biological state of contaminated soils in order to orientate their future use, iv) soil monitoring of biological state at large scale. moreover, it provides communication tools, such as bioindicator technical sheets and web-interface addressed to stakeholders (http://ecobiosoil.univ-rennes1.fr/ademebioindicateur/). conclusion- this10 years research programme, unique at the european union scale, permits to improve the knowledge on soil biodiversity and its functioning, and provides tools to stakeholders for future soil management.},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Rorat2014,

title = {Coelomocyte-derived fluorescence and DNA markers of composting earthworm species},

author = {Agnieszka Rorat and Neli Kachamakova-Trojanowska and Alicja Jozkowicz and Jerzy Kruk and Claude Cocquerelle and Franck Vandenbulcke and Michal Santocki and Barbara Plytycz},

year  = {2014},

date = {2014-01-01},

journal = {Journal of Experimental Zoology Part A: Ecological Genetics and Physiology},

volume = {321},

number = {1},

pages = {28-40},

abstract = {Supravital species identification of morphologically similar syntopic earthworms inhabiting dung and compost heaps or those from commercial cultures is difficult. the aim of the studies was to find out non‐invasive species‐specific markers for proper segregation of earthworm species from a dense mixed colony of waste decomposers. worms were segregated according to external characteristics into eisenia andrei, eisenia fetida, and dendrobaena veneta, and left for reproduction and analysis of non‐invasively retrieved coelomocyte‐containing coelomic fluid and/ or species‐specific partial sequences of cytochrome c oxidase subunit i (coi) gene in dna extracted from amputated tail tips of adults and their offspring. flow cytometric analysis of coelomocyte samples revealed that amount of nuclear dna increases in order d. venetae. andrei<e. fetida, and intensity of eleocyte‐derived fluorescence is lower in d. veneta than in eisenia spp. spectrofluorimetry of coelomocyte lysates revealed that the amount of eleocyte‐stored riboflavin is significantly lower in coelomocyte lysates from d. veneta than from eisenia spp., and the emission peak of x‐fluorophore is much more distinct in d. veneta than in eisenia spp. coelomic fluid of e. andrei exhibits a very distinct spectra of mug fluorophore which are absent in d. veneta and in the majority of e. fetida, while some e. fetida possess mug‐like fluorophore. sequences of the coi gene in the dna of the worms from the mixed colony and their offspring confirmed species identity. in conclusion, species‐specific coelomocyte‐derived markers may be a useful complement to morphological and dna‐based taxonomy during studies on syntopic earthworms},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Rorat2014a,

title = {Mutual interactions between sewage sludge-amended soil and earthworms – comparison between Eisenia fetida and Eisenia andrei composting species},

author = {Agnieszka Rorat and Hanine Suleiman and Anna Grobelak and Malgorzata Kacprzak and Franck Vandenbulcke and Barbara Plytycz},

year  = {2014},

date = {2014-01-01},

booktitle = {International Congress on Water, Waste and Energy Management, 16-18 July 2014, Porto (Portugal)},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Suleiman2014b,

title = {Determining the performance of vermicomposting process according to sewage sludge characteristics and earthworm species},

author = {Hanine Suleiman and Agnieszka Rorat and M Milczarek and Anna Grobelak and Barbara Plytycz and Malgorzata Kacprzak and Franck Vandenbulcke},

year  = {2014},

date = {2014-01-01},

booktitle = {International Congress on Water, Waste and Energy Management, 16-18 July 2014, Porto (Portugal)},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Suleiman2014a,

title = {The investigation of chemical and physical changes and heavy metals content in sewage sludge during vermicomposting},

author = {Hanine Suleiman and Agnieszka Rorat and Barbara Plytycz and Malgorzata Kacprzak and Franck Vandenbulcke},

year  = {2014},

date = {2014-01-01},

booktitle = {Conference Valorisation des boues d’épuration urbaines et problèmes liés, mars 2014, Villeneuve d’Ascq},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Bernard2013,

title = {Gene expression analysis of the genes involved in oxidative stress to explain DNA damages in organisms exposed to cadmium/lead contaminated soils},

author = {Fabien Bernard and Sébastien Lemière and Sylvain Dumez and Franck Brulle and Claude Cocquerelle and Anne Platel and Fabrice Nesslany and Damien Cuny and Annabelle Deram and Franck Vandenbulcke},

year  = {2013},

date = {2013-01-01},

booktitle = {SETAC Europe 23rd Annual Meeting, 12-16 May 2013, Glasgow, Scotland (UK)},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Deram2013,

title = {Higher plants to asses the genotoxicity of soil},

author = {Annabelle Deram and Fabien Bernard and Sylvain Dumez and Caroline Lanier and Sébastien Lemière and Anne Platel and Fabrice Nesslany and Franck Vandenbulcke and Damien Cuny},

year  = {2013},

date = {2013-01-01},

booktitle = {SETAC Europe 23rd Annual Meeting, 12-16 May 2013, Glasgow, Scotland (UK)},

publisher = {Deram, A., Bernard, F., Dumez, S., Lanier, C., Lemière, S., Platel, A., Nesslany, F., Vandenbulcke, F. and Cuny, D. (2013). . In "SETAC Europe 23rd Annual Meeting, May 2013, Glasgow, Scotland UK”.},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Deram2013a,

title = {Complémentarité de modèles végétaux et animaux pour la detection d’effets clastogéniques par le test de comètes},

author = {Annabelle Deram and Sébastien Lemière and Fabien Bernard and Sylvain Dumez and Anne Platel and Fabrice Nesslany and Franck Vandenbulcke and Damien Cuny},

year  = {2013},

date = {2013-01-01},

booktitle = {Rencontres scientifiques de l’Anses, Risques sanitaires liés à la pollution des milieux aériens et hydriques, Restitution du programme national de recherche Environnement Santé Travail, 16 avril 2013, Maisons-Alfort},

abstract = {Objectifs les objectifs de ce programme étaient de mieux apprécier les risques génotoxiques induits par des expositions à des matrices contaminées complexes (sols) et le développement d’outils d’évaluation de la fraction toxique susceptible de porter atteinte à l’adn. la génotoxicité environnementale a été évaluée par le test des comètes permettant la quantification des cassures de l’adn dans des populations cellulaires. ce test a été réalisé, après des expositions aux mêmes sols contaminés, de quatre organismes de niveaux trophiques différents : trifolium repens, brassica oleracea, eisenia fetida et le rat. l’étude concomitante de ces organismes végétaux et animaux devait permettre d’apprécier ainsi la sensibilité et la variabilité des réponses obtenues en termes de détection du caractère génotoxique.},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Lemiere2013,

title = {Comet assay in higher plants and invertebrate to assess the genotoxicity of anthropic contaminated soils},

author = {Sébastien Lemière and Fabien Bernard and Sylvain Dumez and Fabrice Nesslany and Anne Platel and Damien Cuny and Franck Vandenbulcke and Annabelle Deram},

year  = {2013},

date = {2013-01-01},

booktitle = {SEGH 2013 29th International Conference. 8 juillet 2013, Toulouse},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Lemiere2013b,

title = {Hazards of anthropic contaminated soils: what could we learn from environmental genotoxicity markers?},

author = {Sébastien Lemière and Annabelle Deram and Fabien Bernard and Sylvain Dumez and Fabrice Nesslany and Anne Platel and Marion Delattre and Damien Cuny and Franck Vandenbulcke},

year  = {2013},

date = {2013-01-01},

booktitle = {SETAC Europe 23rd Annual Meeting. 12-16 May 2013, Glasgow, Scotland (UK)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Pauwels2013,

title = {Using biomarkers in an evolutionary context: Lessons from the analysis of biological responses of oligochaete annelids to metal exposure},

author = {Maxime Pauwels and Hélène Frérot and Dima Souleman and Franck Vandenbulcke},

year  = {2013},

date = {2013-01-01},

journal = {Environmental Pollution},

volume = {179},

pages = {343-350},

abstract = {Anthropogenic activities may lead to the accumulation of inorganic and organic compounds in topsoils. biota living in close contact with contaminated soils may experience stress at different levels of biological organization throughout the continuum from molecular to community level. biological responses observed at the individual or infra-individual level of biological organization led to the development of biomarkers. the development of biomarkers consists often in evidencing biological modifications following a contaminant stress in laboratory conditions, using naïve organisms and it is sometime proposed to use the biological state of individuals from sentinel species collected in the field to evaluate the level of environmental exposure. however, considering the possibility of local adaptation following long-term exposure, organisms response sampled in the field may substantially differ from laboratory specimens. in this review, we discuss this point focusing on the definition and validity of molecular biomarkers of metal pollution using earthworms of the lumbricidae family.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Peres2013,

title = {Application of soil bioindicators for risk assessment, monitoring and soil characterization in contaminated soils. Results from the French national "Bioindicators Programme"},

author = {Guénola Pérès and Antonio Bispo and Cécile Grand and Daniel Cluzeau and Isabelle Gattin and Mickaël Hedde and Nathalie Cheviron and Jennifer Harris-Hellal and Marina Le-Guédard and Jean-Jacques Bessoule and Nuria Ruiz and Benjamin Pauget and Thierry Béguiristain and Samuel Dequiedt and Rémi Chaussod and Olivier Faure and Adnane Hitmi and Steven Criquet and Marc Legras and Nicolas Laurent and Franck Vandenbulcke and Michaël Coeurdassier and Stéphane Ponton and Jérôme Cortet and Cécile Villenave and Josselin Bodilis and Patrice Lepelletier and Salima Taibi and Jeanne-Chantal Thoisy and Jeanne Bodin},

year  = {2013},

date = {2013-01-01},

booktitle = {AquaConSoil, 16-19 April 2013, Barcelona (Espagne)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Rorat2013,

title = {Soil amendment with municipal sewage sludge affects the immune system of earthworms Dendrobaena veneta},

author = {Agnieszka Rorat and Malgorzata Kacprzak and Franck Vandenbulcke and Barbara Plytycz},

year  = {2013},

date = {2013-01-01},

journal = {Applied Soil Ecology},

volume = {64},

pages = {237-244},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Rorat2013a,

title = {The influence of sewage sludge vermicomposting on Dendrobaena veneta, Eisenia andrei and Eisenia fetida immune system},

author = {Agnieszka Rorat and Hanine Suleiman and M Milczarek and Malgorzata Kacprzak and Franck Vandenbulcke and Barbara Plytycz},

year  = {2013},

date = {2013-01-01},

booktitle = {22th International Symposium of Molecular and Physsiological Aspects of Regulatory Processes of the Organism, 5-6 June 2013, Krakow (Poland)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Suleiman2013,

title = {Comparing different strategies of vermicomposting},

author = {Hanine Suleiman and Agnieszka Rorat and Barbara Plytycz and Malgorzata Kacprzak and Franck Vandenbulcke},

year  = {2013},

date = {2013-01-01},

booktitle = {SETAC Europe 23rd Annual Meeting, 12-16 May 2013, Glasgow, Scotland (UK)},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Suleiman2013a,

title = {The influence of vermicomposting on heavy metals contained in sewage sludge},

author = {Hanine Suleiman and Agnieszka Rorat and Barbara Plytycz and Malgorzata Kacprzak and Franck Vandenbulcke},

year  = {2013},

date = {2013-01-01},

booktitle = {5th Central European Congress of Life Science Eurobiotech, 8-11 October 2013, Krakow (Pologne)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Lemiere2013a,

title = {Environmental genotoxicity of cadmium and/or lead in natural soil, for the earthworm, Eisenia fetida},

author = {Sébastien Lemière and Marion Delattre and Julie Leclercq-Dransart and Fabien Bernard and Sylvain Dumez and Fabrice Nesslany and Anne Platel and Damien Cuny and Annabelle Deram and Franck Vandenbulcke},

year  = {2013},

date = {2013-01-01},

booktitle = {SETAC Europe 23rd Annual Meeting, 12-16 May 2013, Glasgow, Scotland (UK)},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Bernard2012,

title = {Sensibilité du test des comètes et de la mesure de l’expression de gènes cibles pour la mise en évidence de la génotoxicité des contaminations des sols en faibles doses},

author = {Fabien Bernard and Sébastien Lemière and Damien Cuny and Franck Vandenbulcke and Annabelle Deram},

year  = {2012},

date = {2012-01-01},

booktitle = {Colloque IRENI. 23 novembre 2012},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Hedde2012,

title = {Soil fauna used as bioindicators for soil monitoring, risk assessment and soil characterization. Results from the French national “Bioindicators Programme.”},

author = {Mickaël Hedde and Antonio Bispo and Daniel Cluzeau and Michaël Coeurdassier and Jérôme Cortet and Annette De-Vaufleury and Muriel Guernion and Benjamin Pauget and Nuria Ruiz and Franck Vandenbulcke and Cécile Villenave and A Alaphilippe and Thierry Béguiristain and Francis Douay and Olivier Faure and H Hitmi and Sabine Houot and Marc Legras and Jean-François Vian and S Conil and Laurence Rougé and Guénola Pérès},

year  = {2012},

date = {2012-01-01},

booktitle = {XVI ICSZ – International Colloquium on Soil Zoology, 6-10 August 2012, Coimbra (Portugal)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Lemiere2012,

title = {Complémentarité des modèles Brassica, Eisenia et Trifolium pour l’évaluation de la génotoxicité environnementale des sols},

author = {Sébastien Lemière and Fabien Bernard and Franck Vandenbulcke and Fabrice Nesslany and Anne Platel and Sylvain Dumez and Damien Cuny and Annabelle Deram},

year  = {2012},

date = {2012-01-01},

booktitle = {11èmes Journées d’Etude des Sols, colloque AFES: Le sol face aux changements globaux.19-23 mars 2012, Versailles},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Manier2012,

title = {Biomarker measurements in Trifolium repens and Eseinia fetida to assess the toxicity of soil contaminated with leachate of landfill: a microcosm study},

author = {Nicolas Manier and Franck Brulle and Franck Le-Curieux and Franck Vandenbulcke and Annabelle Deram},

year  = {2012},

date = {2012-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {80},

pages = {339-348},

abstract = {To assess the toxicity of a soil contaminated with landfill leachate, biomarker measurements in two species living in close contact with the soil, i.e. a plant species trifolium repens and an animal species eisenia fetida, were conducted. briefly, both species were studied after simultaneous exposure conducted in microcosms. the organisms were exposed to soil supplemented with pure leachate, leachate diluted to 50%; leachate diluted to 25% and without leachate. after a 10 weeks exposure period, we observed an increase in the olive trail moment in t. repens, compared to the reference, for 50% and pure leachate. the response observed appears to be dose-dependent and linear in our experimental conditions. addition of the leachate to the reference soil induced an increase in cd-metallothionein-coding mrna quantity in e. fetida. in addition, expression level of another gene implied in detoxification and coding phytochelatin synthase was significantly induced in worms exposed to the reference soil spiked with the leachate, regardless presence of t. repens. thus, t. repens and e. fetida can be used in a complementary manner to assess soil quality. sensitivities of the test species yield sensitive bioassays as both species responded at low doses despite the buffering effect of the soil. (c) 2012 elsevier inc.. all rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Peres2012c,

title = {Earthworm indicators as tools for soil monitoring, characterization and risk assessment. An example from the national Bioindicator programme (France)},

author = {Guénola Pérès and Franck Vandenbulcke and Muriel Guernion and Mickaël Hedde and Thierry Béguiristain and Francis Douay and Sabine Houot and Denis Piron and Antoine Richard and Antonio Bispo and Cécile Grand and Laurence Galsomies and Daniel Cluzeau},

year  = {2012},

date = {2012-01-01},

journal = {Pedobiologia},

volume = {54},

pages = {S77-S87},

abstract = {Considering the limited number of bioindicators available to assess soil quality, a national research programme was set up in france to develop such indicators (2006-2012), the "bioindicator" programme.this programme tested 47 biological parameters (i.e. microorganisms, fauna, flora) including earthworms, in several sites differing in terms of land use, contamination type - pahs or metals - and pollution levels. the present study proposes some study objectives for bioindicator approaches, based on the earthwormn results from the programme. therefore, different earthworm descriptors were tested at the community level (e.g. abundance, biomass, species and functional structures, and ecological traits) as well as the organism level (i.e. measuring the metallothionein coding gene expression level in earthworms). the present results, obtained from the programme's spring 2009 sampling campaign, discriminated among the different descriptors and showed that earthworm and endogeic abundance as well as the individual weight of endogeics seem to be good indicators in non-contaminated (cultivated) sites, while the ecological structure, namely the proportion of anecic vs. endogeic species, and the proportion of non-vulnerable species should be used as indicators of contaminated soils. furthermore, the first results obtained for lumbricus terrestris and l rubellus rubellus are encouraging as they show that metallothionein expression increases in metal-contaminated soils. the relevance of these descriptors, which have to be considered in study objectives, requires the analysis of 2010 results. (c) 2011 elsevier gmbh. all rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Suleiman2012,

title = {Comparing different strategies of vermicomposting; towards a modelling of this process},

author = {Hanine Suleiman and Agnieszka Rorat and Barbara Plytycz and Malgorzata Kacprzak and Franck Vandenbulcke},

year  = {2012},

date = {2012-01-01},

booktitle = {4th International Conference on Advances in Sustainable Sewage Sludge Management. 3-5 December 2012, Szczyrk (Pologne)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Lemiere2012a,

title = {Génotoxicité environnementale du cadmium et du plomb, seuls ou en mélange, en sol naturel, pour le ver Eisenia fetida},

author = {Sébastien Lemière and Marion Delattre and Julie Leclercq-Dransart and Florent Occeli and Fabien Bernard and Sylvain Dumez and Fabrice Nesslany and Anne Platel and Damien Cuny and Annabelle Deram and Franck Vandenbulcke},

year  = {2012},

date = {2012-01-01},

booktitle = {Colloque SEFA, 4-5 juillet 2012, Lyon},

abstract = {En toxicologie génétique, le cadmium est un génotoxique reconnu et les mécanismes expliquant son caractère clastogène sont indirects : par l’induction d’espèces réactives de l’oxygène et/ou l’inhibition des mécanismes de réparation de l’adn. pour le plomb, l’ensemble des travaux publiés sont équivoques et ambigus voire contradictoires. concernant les effets clastogènes et mutagènes parfois reportés, ses mécanismes d’action ne sont pas clairs et probablement indirects. en génotoxicité environnementale, pour ses deux métaux, l’étude de leur caractère génotoxique, seuls ou en mélange et en sols naturels, est encore plus complexe car il faut également considérer: (1) les notions de disponibilité environnementale, de biodisponibilités environnementale et toxicologique, (2) leurs présences simultanées dans les sites et sols contaminés ou pollués (particulièrement dans le contexte régional du nord-pas-de-calais), (3) et alors leurs éventuelles interactions toxicologiques. le test des comètes est une technique de microélectrophorèse de noyaux de cellules isolées qui permet l’étude des dommages primaires à l’adn (cassures double et simple brin, sites alcali-labiles) au sein d’une population cellulaire. cet essai a été mis au point et validé avec les coelomocytes de notre modèle biologique, reconnu en écotoxicité des sols, eisenia fetida. dans une première phase d’expérimentation, nous avons réalisé des expositions in vivo (3 & 10 jours) en utilisant un sol péri-urbain caractérisé (et représentatif du contexte régional) artificiellement contaminé par du cadmium et/ou du plomb, à des concentrations environnementales et réalistes (entre 2,5 et 20 mg/kg de sol sec pour le cadmium et entre 250 et 2000 mg/kg pour le plomb, gammes de concentrations de raison 2). les principaux résultats, qui seront présentés de manière détaillée dans cette communication, sont les suivants : (1) nous observons des dommages à l’adn significatifs suite aux expositions au cadmium seul, sans relations temps-dépendante et/ou dose-dépendante ; (2) suite aux expositions au plomb, une légère augmentation du niveau de dommages à l’adn est observée, mais celle-ci n’est jamais significative : le plomb n’apparait pas comme génotoxique, plus précisément comme clastogène pour notre ver de terre ; (3) les dommages observés suite aux expositions aux différents mélanges cd/pb sont intermédiaires entre les cassures reportées suite aux expositions monométalliques. plusieurs hypothèses pourraient expliquer ces résultats dans le cas des expositions aux mélanges cd/pb (et des résultats complémentaires sont en cours d’acquisition pour les étudier): (1) une mise en place de mécanismes de défense contre les effets toxiques de pb, qui protégerait également les individus contre la génotoxicité de cd ; (2) des effets autres que clastogènes de pb qui masquerait la génotoxicité de cd ; (3) une faible biodisponibilité de pb dans le sol utilisé. a terme, des travaux avec des échantillons environnementaux (i.e. des sols contaminés prélevés in situ) seront également réalisés et confrontés aux résultats obtenus au laboratoire.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Brulle2011,

title = {Gene expression analysis of 4 biomarker candidates in Eisenia fetida exposed to an environmental metallic trace elements gradient: A microcosm study},

author = {Franck Brulle and Sébastien Lemière and Christophe Waterlot and Francis Douay and Franck Vandenbulcke},

year  = {2011},

date = {2011-01-01},

journal = {Science of the Total Environment},

volume = {409},

number = {24},

pages = {5470-5482},

abstract = {Past activities of 2 smelters (metaleurop nord and nyrstar) led to the accumulation of high amounts of metal trace elements (tes) in top soils of the noyelles-godault/auby area, northern france. earthworms were exposed to polluted soils collected in this area to study and better understand the physiological changes, the mechanisms of acclimation, and detoxification resulting from te exposure. previously we have cloned and transcriptionally characterized potential biomarkers from immune cells of the ecotoxicologically important earthworm species eisenia fetida exposed in vivo to te-spiked standard soils. in the present study, analysis of expression kinetics of four candidate indicator genes (cadmium-metallothionein, coactosin like protein, phytochelatin synthase and lysenin) was performed in e. fetida after microcosm exposures to natural soils exhibiting an environmental cadmium (cd) gradient in a kinetic manner. te body burdens were also measured. this microcosm study provided insights into: (1) the ability of the 4 tested genes to serve as expression biomarkers, (2) detoxification processes through the expression analysis of selected genes, and (3) influence of land uses on the response of potential biomarkers (gene expression or te uptake). (c) 2011 elsevier b.v. all rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Laborie2011,

title = {Maternal perinatal undernutrition has long-term consequences on morphology, function and gene expression of the adrenal medulla in the adult male Rat},

author = {Christine Laborie and Olivier Molendi-Coste and Christophe Breton and Valérie Montel and Franck Vandenbulcke and Luca Grumolato and Youssef Anouar and Didier Vieau},

year  = {2011},

date = {2011-01-01},

journal = {Journal of Neuroendocrinology},

volume = {23},

number = {8},

pages = {711-724},

abstract = {Epidemiological studies suggest that maternal undernutrition sensitises to the development of chronic adult diseases, such as type 2 diabetes, hypertension and obesity. although the physiological mechanisms involved in this ‘perinatal programming’ remain largely unknown, alterations of stress neuroendocrine systems such as the hypothalamic-pituitary-adrenal (hpa) and sympathoadrenal axes might play a crucial role. despite recent reports showing that maternal perinatal undernutrition disturbs chromaffin cells organisation and activity in male rats at weaning, its long-term effects on adrenal medulla in adult animals are unknown. using a rat model of maternal perinatal 50% food restriction (fr50) from the second week of gestation until weaning, histochemistry approaches revealed alterations in noradrenergic chromaffin cells aggregation and in cholinergic innervation in the adrenal medulla of 8-month-old fr50 rats. electron microscopy showed that chromaffin cell granules exhibited ultrastructural changes in fr50 rats. these morphological changes were associated with reduced circulating levels and excretion of catecholamines. by contrast, catecholamine plasma levels were significantly increased after a 16 or 72 h of fasting, indicating that the responsiveness of the sympathoadrenal system to food deprivation was accentuated in fr50 adult rats. among 384 pituitary adenylate cyclase-activating polypeptide-sensitive genes, we identified 129 genes (33.6%) that were under expressed (ratio < 0.7) in fr50 animals. a large number of these genes are involved in cytoskeleton remodelling and vesicle trafficking. taken together, our results show that maternal perinatal undernutrition programmes adrenomedullary function and gene expression in adult male rats. because catecholamines contribute to metabolic homeostasis, as well as arterial blood pressure regulation, the alterations observed in the adrenal medulla of adult male fr50 rats may participate in the programming of chronic adult diseases},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Bernard2010,

title = {Metallic trace element body burdens and gene expression analysis of biomarker candidates in Eisenia fetida, using an ''exposure/depuration'' experimental scheme with field soils},

author = {Fabien Bernard and Franck Brulle and Francis Douay and Sébastien Lemière and Sylvain Demuynck and Franck Vandenbulcke},

year  = {2010},

date = {2010-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {73},

number = {5},

pages = {1034-1045},

abstract = {Smelting plant activities lead to the accumulation of metal trace elements (mtes) in soils. the presence of high concentrations of mtes can generate an environmental stress likely to affect macroinvertebrates living in close soil contact such as the annelida oligochaeta. eisenia fetida, an ecotoxicologically important test species, was successively exposed to two field soils: (1) a highly contaminated agricultural topsoil collected near the former smelter metaleurop nord (noyelles-godault, france) which contaminated surrounding soils by its atmospheric emissions [exposure phase], and then (2) a slightly contaminated topsoil from an urban garden located in the conurbation of lille (wambrechies) [depuration phase]. two analyses were performed during each phase. firstly, the gene expression levels of four biomarker candidates identified in previous studies were analyzed in e. fetida coelomocytes. these candidates are cd-metallothionein, phytochelatin synthase, coactosin-like protein and lysenin. secondly, the body burdens of the following elements cd, pb, zn, cu, fe, ca, and p were measured. moreover, both analyses were also performed in lumbricus rubellus, an annelid species collected from the two tested soil-originating sites. analysis of gene expression and mte body burdens in both species are discussed to: (1) evaluate expression biomarkers; (2) gain insight the detoxification processes and the long-term response to a metallic stress and (3) compare the responses observed in a test species (e. fetida) with the responses of a field species (l. rubellus). (c) 2010 elsevier inc. all rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Brulle2010a,

title = {Identification and analysis of the expression profiles of new biomarker candidates in Eisenia fetida: A transcriptomic approach},

author = {Franck Brulle and Claude Cocquerelle and Sébastien Lemière and Francis Douay and Alain Leprêtre and Franck Vandenbulcke},

year  = {2010},

date = {2010-01-01},

booktitle = {SETAC Europe 20th Annual Meeting. 23-27 May 2010, Seville (Espagne)},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Brulle2010b,

title = {Transcriptomic underpinning of toxicant-mediated physiological function alterations in three terrestrial invertebrate taxa: A review},

author = {Franck Brulle and Andrew-John Morgan and Claude Cocquerelle and Franck Vandenbulcke},

year  = {2010},

date = {2010-01-01},

journal = {Environmental Pollution},

volume = {158},

number = {9},

pages = {2793-2808},

abstract = {Diverse anthropogenic activities often lead to the accumulation of inorganic and organic residues in topsoils. biota living in close contact with contaminated soils may experience stress at different levels of biological organisation throughout the continuum from the molecular-genetic to ecological and community levels. to date, the relationship between changes at the molecular (mrna expression) and biochemical/physiological levels evoked by exposures to chemical compounds has been partially established in a limited number of terrestrial invertebrate species. recently, the advent of a family of transcriptomic tools (e.g. real-time pcr, subtractive suppressive hybridization, expressed sequence tag sequencing, pyro-sequencing technologies, microarray chips), together with supporting informatic and statistical procedures, have permitted the robust analyses of global gene expression changes within an ecotoxicological context. this review focuses on how transcriptomics is enlightening our understanding of the molecular-genetic responses of three contrasting terrestrial macroinvertebrate taxa (nematodes, earthworms, and springtails) to inorganics, organics, and agrochemicals},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Cocquerelle2010,

title = {Identification of gene transcripts differentially expressed in the immune competent cells (coelomocytes) of Eisenia fetida exposed to metallic pollution},

author = {Claude Cocquerelle and Ben Elsworth and Franck Brulle and A.John Morgan and M Blaxter and Franck Vandenbulcke},

year  = {2010},

date = {2010-01-01},

booktitle = {International Symposium on Earthworm Ecology (ISEE), 5-10 September 2010, Xalapa, Veracruz (Mexico)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Elsworth2010,

title = {Identification of gene transcripts differentially expressed during metallic exposure in the immune circulating cells, namely coelomocytes, from Eisenia fetida exposed to metallic pollution},

author = {Ben Elsworth and Claude Cocquerelle and Franck Brulle and M Blaxter and Franck Vandenbulcke},

year  = {2010},

date = {2010-01-01},

booktitle = {SETAC Europe 20th Annual Meeting, 23-27 May 2010, Seville (Spain)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Hedde2010,

title = {Recherche d’outils de surveillance, de caractérisation et d’évaluation des risques des sols},

author = {Mickaël Hedde and Antonio Bispo and Daniel Cluzeau and Jérôme Cortet and Francis Douay and Laurence Galsomies and Cécile Grand and Sabine Houot and Corinne Leyval and Benjamin Pauget and Nuria Ruiz-Camacho and Erwin Ulrich and Franck Vandenbulcke and Annette de-Vaufleury and Cécile Villenave and Guénola Pérès},

year  = {2010},

date = {2010-01-01},

booktitle = {Programme ADEME - Bioindicateur II : bio-indicateurs basés sur la faune du sol. Colloque de la Société Française d’Ecotoxicologie Fondamentale et Appliquée},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Homa2010,

title = {Metal-specific effects on metallothionein gene induction and riboflavin content in coelomocytes of Allolobophora chlorotica},

author = {Joanna Homa and Malgorzata Klimek and Jerzy Kruk and Claude Cocquerelle and Franck Vandenbulcke and Barbara Plytycz},

year  = {2010},

date = {2010-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {73},

number = {8},

pages = {1937-1943},

abstract = {Metal pollution affects earthworm coelomocytes, including their differential counts, riboflavin content and metallothioneins (mt) involved in metal homoeostasis and detoxification. the present work shows effects of ni, cu, zn, cd, and pb at the same molarity (1 mm) on coelomocytes of allolobophora chlorotica after 2-day worm dermal exposure to metal chlorides. numbers of coelomocytes/eleocytes extruded by electric shock and amounts of riboflavin in coelomocyte lysates were significantly decreased in cu-exposed worms, less diminished in response to ni, zn, cd, and unaffected by pb. in sharp contrast, real-time pcr revealed a very strong (272 fold) mt-mrna induction in response to cd only. the induction was very low in response to zn, cu, pb, and ni ions (2.6, 2.1, 1.4, and 1.3-fold, respectively). in conclusion, decreased cell counts and riboflavin content are molecular biomarkers of cu exposure while induction of mt-mrna is a molecular biomarker of worm cd exposure},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Lemiere2010a,

title = {Indoor microcosm studies for ecotoxicological evaluation of metal contaminated soil remediation agents},

author = {Sébastien Lemière and Jean-Philippe Godet and Marion Tanguy and Fabien Grumiaux and Régine Leroux and Franck Vandenbulcke and Sylvain Demuynck and Céline Pernin and Christelle Pruvot and Patrick Delplace and Francis Douay and Alain Leprêtre},

year  = {2010},

date = {2010-01-01},

booktitle = {SETAC Europe 20th Annual Meeting. 23-27 May 2010, Seville (Espagne)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Peres2010,

title = {The use of earthworms as tool for soil monitoring, characterization and risk assessment. Example of a Bioindicator Programme developed at National scale (France)},

author = {Guénola Pérès and Franck Vandenbulcke and Muriel Guernion and Denis Piron and Laurence Rougé and Mickaël Hedde and Antonio Bispo and Laurence Galsomies and Cécile Grand and Daniel Cluzeau},

year  = {2010},

date = {2010-01-01},

booktitle = {International Symposium on Earthworm Ecology (ISEE). 5-10 September 2010, Xalapa, Veracruz (Mexico)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Bernard2009,

title = {Metallic Trace Elements body burden and gene expression analysis of biomarker candidates in Eisenia fetida, during an “intox-detox” protocol performed with field soils},

author = {Fabien Bernard and Franck Brulle and Francis Douay and Sébastien Lemière and Sylvain Demuynck and Alain Leprêtre and Franck Vandenbulcke},

year  = {2009},

date = {2009-01-01},

booktitle = {SETAC Europe 19th Annual Meeting, 31 May-4 June 2009, Göteborg (Suède)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Brulle2009a,

title = {Développement de biomarqueurs d’exposition aux métaux chez un organisme test: l’Annélide Oligochète Eisenia fetida},

author = {Franck Brulle and Claude Cocquerelle and Francis Douay and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},

year  = {2009},

date = {2009-01-01},

booktitle = {2ème Rencontres Nationales de la Recherche sur les Sites & Sols Pollués, ADEME. 20-21 octobre 2009, Paris},

abstract = {Couches supérieures des sols, où les concentrations peuvent être largement supérieures aux teneurs habituelles régionales. les annélides oligochètes sont présents dans ces sols pollués bien qu’ils présentent une sensibilité exacerbée aux métaux. très peu de choses étant connues quant à l’identification et la mise en place des mécanismes de réponse à ces métaux au niveau moléculaire, le travail de caractérisation des candidats biomarqueurs de pollution a été réalisé à partir des cellules immunitaires du ver eisenia fetida, une espèce modèle recommandée en écotoxicologie. deux approches ont été mises en place. une première approche, qualifiée de ciblée et basée sur la conservation phylogénique observée entre espèces, a permis l’identification de plusieurs candidats. une deuxième approche, dite globale et consistant en la construction de banques soustractives a permis d’identifier des gènes dont l’expression est affectée lors d’une exposition à un mélange complexe de métaux. enfin, les candidats biomarqueurs de pollution métallique mis en lumière par les deux approches ont été mesurés sur des animaux exposés à des sols pollués de longue date par des activités métallurgiques. ce travail a été réalisé dans le cadre d’une thèse de doctorat (f. brulle) financée par l’ademe et la région nord-pas-de-calais. 1- premièrement, nous avons cloné et analysé le niveau d’expression de 14 candidats biomarqueurs chez le modèle de laboratoire eisenia fetida exposé in vivo à des sols standards contaminés par le cadmium. les 14 candidats ont été choisis parmi des gènes codant des protéines fortement conservées pour lesquelles des variations d’expression génique ont été rapportées dans la littérature, lors d’exposition de macroinvertébrés à des etms. le niveau d’expression des gènes a été mesuré dans les coelomocytes (cellules immunitaires) puisque la majorité des effecteurs sélectionnés sont connus pour leur implication dans des mécanismes de défense [1].},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Brulle2009b,

title = {Développement de biomarqueurs d'exposition aux métaux chez un organisme test: l'Annélide Oligochète Eisenia fetida},

author = {Franck Brulle and Claude Cocquerelle and Francis Douay and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},

year  = {2009},

date = {2009-01-01},

booktitle = {15ème Colloque National de la Recherche en IUT, CNRIUT 2009. 8-10 juin 2009, Villeneuve d’Ascq},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Brulle2009c,

title = {Gene expression analysis of 4 biomarker candidates in Eisenia fetida exposed to an environmental cadmium gradient: a microcosm study},

author = {Franck Brulle and Francis Douay and Alain Leprêtre and Franck Vandenbulcke},

year  = {2009},

date = {2009-01-01},

booktitle = {SETAC Europe 19th Annual Meeting, 31 May-4 June 2009, Göteborg (Suède)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Brulle2009d,

title = {Développement de biomarqueurs d'exposition aux métaux basés sur les fonctions physiologiques de l'annélide oligochète Eisenia fetida},

author = {Franck Brulle and Franck Vandenbulcke},

year  = {2009},

date = {2009-01-01},

journal = {Etude et Gestion des Sols},

volume = {16},

number = {3/4},

pages = {159-174},

abstract = {Les activités métallurgiques entraînent i'accumulation d'éléments traces métalliques dans les couches superficielles des sols, où l'on peut observer des concentrations largement supérieures au fond pédogéochimique et aux normes en vigueur. la contamination des sols est une menace pour la santé publique et la présence de grandes quantités d'éléments traces métalliques peut générer un stress susceptible d'affecter les organismes exposés à des sols pollués. les annélides oligochètes vivent en contact étroit avec ces sols pollués et sont parmi les organismes vivants présentant une sensibilité exacerbée aux métaux. très peu de choses sont connues quant à i'identification et la mise en place des mécanismes de réponse à ces métaux au niveau moléculaire. en exploitant la conservation phylogénique observée entre espèces, nous avons été capables de cloner et de caractériser un ensemble de biomarqueurs potentiels à partir des coelomocytes de i'annélide oligochète eisenia fetida, une espèce modèle recommandée en écotoxicologie. deux approches ont été mises en place. premièrement, une approche qualifiée de ciblée, consistant à identifier tous les effecteurs parmi des protéines fortement conservées pour lesquels une variation lors d'une exposition métallique était reportée dans la littérature. deuxièmement, nous avons entrepris une approche, qualifiée de globale, consistant en la construction de banques soustractives pour identifier chez eisenia fetida les gènes dont l'expression est affectée lors d'une exposition à un mélange complexe de métaux, représentatif d'un site naturel fortement contaminé. ces deux approches ont permis i'identification de 4 candidats biomarqueurs de pollution métallique.},

note = {ACLN},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Gueguen2009,

title = {Oyster hemocytes express a proline-rich peptide displaying synergistic antimicrobial activity with a defensin},

author = {Yannick Gueguen and Bernard Romestand and Julie Fievet and Paulina Schmitt and Delphine Destoumieux-Garzón and Franck Vandenbulcke and Philippe Bulet and Evelyne Bachère},

year  = {2009},

date = {2009-01-01},

journal = {Molecular Immunology},

volume = {46},

number = {4},

pages = {516-522},

abstract = {A cdna sequence that encodes a 61-amino acid polypeptide precursor with homologies to proline-rich antimicrobial peptides (amps) was identified in the oyster crassostrea gigas. after release of a hydrophobic signal peptide, the resulting 37-amino acid peptide, cg-prp, is composed of an acidic region and a cationic proline-rich region. to evaluate the biological properties of cg-prp, multiple proline-rich peptides corresponding to putative processing of the full-length cg-prp were synthesized. a limited antimicrobial activity was observed for two of them, which also showed strong synergistic antimicrobial activity with cg-def, a defensin from c. gigas. to our knowledge, this is the first evidence of synergy between a defensin and another amp in an invertebrate. by in situ hybridization, the expression of cg-prp was found to be restricted to hemocytes and induced following bacterial challenge. cg-prp transcripts were also detected in hemocytes infiltrating mantle, where cg-def is expressed. additionally, by immunocytochemistry, we showed that cg-prp or one of its variants is present in some hemocytes together with defensins. in conclusion, we described here the first proline-rich amp from mollusk. from our study, it is likely to provide a first line of defense against bacterial invasion by acting through synergy with defensins.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Lemiere2009,

title = {Utilisation de lombricides exposés en microcosms pour évaluer l’intérêt d’agents immobilisants pour la remediation de sols urbains contaminés par les métaux},

author = {Sébastien Lemière and Fabien Grumiaux and Jean-Philippe Godet and Marion Tanguy and Patrick Delplace and Sylvain Demuynck and Franck Brulle and Christelle Pruvot and Franck Vandenbulcke and Francis Douay and Alain Leprêtre},

year  = {2009},

date = {2009-01-01},

booktitle = {2ème Rencontres Nationales de la Recherche sur les Sites & Sols Pollués, ADEME. 20-21 octobre 2009, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke2009,

title = {Identification and analysis of the expression profiles of new biomarker candidates in Eisenia fetida: a transcriptomic approach},

author = {Franck Vandenbulcke},

year  = {2009},

date = {2009-01-01},

booktitle = {International Symposium of Risk Assessment (ISTA). 30 août-3 septembre 2009, Metz},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Brulle2008,

title = {Identification and expression profile of gene transcripts differentially expressed during metallic exposure in Eisenia fetida coelomocytes},

author = {Franck Brulle and Claude Cocquerelle and Guillaume Mitta and Vincent Castric and Francis Douay and Alain Leprêtre and Franck Vandenbulcke},

year  = {2008},

date = {2008-01-01},

journal = {Developmental and Comparative Immunology},

volume = {32},

pages = {1441-1453},

abstract = {The aim of this work was to identify in eisenia fetida genes whose expression are regulated following exposure to a complex mixture of metallic trace elements (mte) representative of a highly polluted smelter soil. suppression subtractive hybridization (ssh) was used to construct cdna libraries enriched in up- or down-regulated transcripts in the immunecirculating cells of the coelomic cavities, namely coelomocytes, from worms exposed to metallic pollution. among 1536 ssh-derived cdna clones sequenced, we identified 764 unique ests of which we selected 18 candidates on the basis of their redundancy. these selected candidates were subjected to a two-step validation procedure based on the study of their expression level by real-time pcr. the first step consisted in measuring the expression of the 18 candidates in worms exposed to artificial contaminated soil. the second step consisted in measuring the expression in animals exposed to a ‘‘naturally’’ contaminated soil sampled close to a smelter. both steps allowed us to highlight 3 candidates that are strongly induced in worms exposed to a smelter polluted soil. these candidates are: the well-known mte-induced cd–metallothionein and 2 original biomarkers, lysenin, and a transcript, which cloning of the complete coding sequence identified as the coactosin-like protein.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Brulle2008b,

title = {Identification and expression profile of gene transcripts differentially expressed during metallic exposure in Eisenia fetida},

author = {Franck Brulle and Claude Cocquerelle and Guillaume Mitta and Vincent Castric and Francis Douay and Alain Leprêtre and Franck Vandenbulcke},

year  = {2008},

date = {2008-01-01},

booktitle = {SETAC Europe 18th Annual Meeting, 25-29 May 2008, Krakow (Poland)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Brulle2008c,

title = {Identification and analysis of the expression profiles of new biomarker candidates to gain insight the immune response to a metallic stress},

author = {Franck Brulle and Claude Cocquerelle and Guillaume Mitta and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},

year  = {2008},

date = {2008-01-01},

booktitle = {SETAC Europe 18th Annual Meeting. 25-29 May 2008, Krakow (Pologne)},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Brulle2008a,

title = {CDNA cloning and expression analysis of Eisenia fetida (Annelida:Oligochaeta) phytochelatin synthase under cadmium exposure},

author = {Franck Brulle and Claude Cocquerelle and Atta-Nda Wamalah and Andrew-John Morgan and Peter Kille and Alain Leprêtre and Franck Vandenbulcke},

year  = {2008},

date = {2008-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {71},

number = {1},

pages = {47-55},

abstract = {Metallothioneins (mts) are central to trace metal homeostasis and detoxification throughout biological systems. prokaryotes, plants, and fungi utilize both gene encoded cysteine-rich polypeptides (classically designated class i and ii mts) and enzymatically synthesized cysteinerich peptides (classically designated class iii mts or phytochelatins). in contrast, although gene encoded mts are ubiquitous in animal species the identification of a functional phytochelatin synthase in the nematode caenorhabditis elegans, a representative member of the ecdysozoa, provided the first evidence for these metal-binding peptides in animals. by exploiting the conservation observed between species we have been able to clone and transcriptionally characterize a phytochelatin synthase from the immune cells of the earthworm eisenia fetida, the first evidence for its presence in a phylum belonging to the lophototrochozoa. the complete coding sequence of this enzyme was determined and the phylogenetic relationship to plant, yeast and nematode enzymes elucidated. temporal- and dose-profiling of the transcriptional regulation of phytochelatin synthase and mt in response to cadmium was performed by using real-time pcr.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Lefebvre2008,

title = {Cathepsin L and cystatin B gene expression discriminates immune cœlomic cells in the leech Theromyzon tessulatum},

author = {Christophe Lefebvre and Franck Vandenbulcke and Béatrice Bocquet and Aurélie Tasiemski and Annie Desmons and Mathilde Vierstraete and Michel Salzet and Claude Cocquerelle},

year  = {2008},

date = {2008-01-01},

journal = {Developmental and Comparative Immunology},

volume = {32},

number = {7},

pages = {795-807},

abstract = {Previous studies evidenced that cystatin b-like gene is specifically expressed and induced in large circulating coelomic cells following bacterial challenge in the leech theromyzon tessulatum. in order to understand the role of that cysteine proteinase inhibitor during immune response, we investigated the existence of members of cathepsin family. we cloned a cathepsin l-like gene and studied its tissue distribution. immunohistochemical studies using anti-cathepsin l and anti-cystatin b antibodies and ultrastructural results demonstrated the presence of three distinct coelomic cell populations: (1) the chloragocytes, which were initially defined as large coelomocytes, (2) the granular amoebocytes and (3) small coelomic cells. among those cells, while chloragocytes contain cystatin b and cathepsin l, granular amoebocytes contain only cathepsin l and the third cell population contains neither cathepsin nor inhibitor. finally, results evidenced that cathepsin l immunopositive granular amoebocytes are chemoattracted to the site of injury and phagocyte bacteria},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Lemiere2008,

title = {Eisenia fetida responses to metal immobilizing agents for contaminated soil remediation: a microcosm evaluation},

author = {Sébastien Lemière and Jean-Philippe Godet and Fabien Grumiaux and Patrick Delplace and Sylvain Demuynck and Franck Vandenbulcke and Francis Douay and Alain Leprêtre},

year  = {2008},

date = {2008-01-01},

booktitle = {SETAC Europe 18th Annual Meeting. 25-29 May 2008, Krakow (Pologne)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Lemiere2008a,

title = {An indoor microcosm study with zebra mussels of metalcontaminated sediment resupension},

author = {Sébastien Lemière and Didier Técher and Patrick Delplace and C Gilliers and Sylvain Demuynck and Franck Vandenbulcke and Alain Leprêtre},

year  = {2008},

date = {2008-01-01},

booktitle = {SETAC Europe 18th Annual Meeting, 25-29 May 2008, Krakow (Poland)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Munoz2008,

title = {Analisis de localizacion y distribucion de ARNm de péptidos antimicrobianos expresados en células de camaron por hibridación in situ},

author = {Marcelo Munoz and Evelyne Bachère and Guillaume Mitta and Franck Vandenbulcke},

year  = {2008},

date = {2008-01-01},

journal = {Revista Tecnológica ESPOL},

volume = {21},

number = {1},

pages = {83-90},

abstract = {La presente publicación describe adaptaciones a métodos para la detección y localización de arnmensajeros de penaeidins, péptidos antimicrobianos del camarón penaeus vannamei, utilizandomarcadores fríos y calientes tanto para muestras correspondientes a células citocentrifugadas como paratejidos incluidos en parafina. si se describen los procedimientos y adaptaciones para realizar lostratamientos previos a la hibridación en los cuales se determinan los pasos para desparafinar tejidos,tiempos de post fijación y tratamiento con arnasa para preservar las condiciones de los tejidos para unabuena observación luego de la hibridación. además, se establecen los mejores parámetros para prepararribosondas y se discuten las ventajas de la utilización de este tipo de sondas con respecto a las sondas dedoble cadena de adn. finalmente se determinan los pasos optimizados para realizar una hibridación ydetección de las ribosondas marcadas a la forfatasa alcalina que permitan la mejor definición ysensibilidad en las células objetivo.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Vandenbulcke2008,

title = {Transcriptomic study of Eisenia fetida immune cells},

author = {Franck Vandenbulcke},

year  = {2008},

date = {2008-01-01},

booktitle = {French-Hungarian Scientific meeting. 1-2 December 2008, Budapest (Hongrie)},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Brulle2007,

title = {The strong induction of metallothionein gene following cadmium exposure transiently affects the expression of many genes in Eisenia fetida: A trade-off mechanism?},

author = {Franck Brulle and Guillaume Mitta and Régine Leroux and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},

year  = {2007},

date = {2007-01-01},

journal = {Comparative Biochemistry and Physiology C-Toxicology & Pharmacology},

volume = {144},

number = {4},

pages = {334-341},

abstract = {Metal pollution causes disturbances at various levels of biological organization in most species. important physiological functions could be affected in the exposed individuals and among the main physiological functions, immunity may provide one (or more) effector(s) whose expression can be directly affected by a metal exposure in various macroinvertebrates. protein expressions were studied in order to test them as molecular biomarkers of metal exposure in eisenia fetida. selected effectors were calmodulin, heat shock proteins, superoxide dismutase, catalase, metallothionein, beta-adrenergic receptor kinase, pyruvate carboxylase, transcriptionally controlled tumor protein, protein kinase c, ubiquitin and cyclophilin-a. the level of expression of each gene was analysed in whole organism following exposures to cadmium in soil using real-time pcr. metallothionein, transcriptionally controlled tumor protein and cyclophilin-a expression were also measured following copper exposures in soil because these genes seemed to be sensitive to copper. this work enabled to distinguish metallothionein and cyclophilin-a among the 15 selected effectors. a strong decrease of the number of transcripts was also detected for most effectors soon after the exposure to cadmium suggesting that a trade-off mechanism occurs},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Gonzalez2007,

title = {Evidence of a bactericidal permeability increasing protein in an invertebrate, the Crassostrea gigas Cg-BPI},

author = {Marcelo Gonzalez and Yannick Gueguen and Delphine Destoumieux-Garzón and Bernard Romestand and Julie Fievet and Martine Pugnière and Françoise Roquet and Jean-Michel Escoubas and Franck Vandenbulcke and Ofer Levy and Laure Sauné and Philippe Bulet and Evelyne Bachère},

year  = {2007},

date = {2007-01-01},

journal = {Proceedings of the National Academy of Sciences of the United States of America},

volume = {104},

number = {45},

pages = {17759-17764},

abstract = {A cdna sequence with homologies to members of the lps-binding protein and bactericidal/permeability-increasing protein (bpi) family was identified in the oyster crassostrea gigas. the recombinant protein was found to bind lps, to display bactericidal activity against escherichia coli, and to increase the permeability of the bacterial cytoplasmic membrane. this indicated that it is a bpi rather than an lps-binding protein. by in situ hybridization, the expression of the c. gigas bpi (cg-bpi) was found to be induced in hemocytes after oyster bacterial challenge and to be constitutive in various epithelia of unchallenged oysters. thus, cg-bpi transcripts were detected in the epithelial cells of tissues/organs in contact with the external environment (mantle, gills, digestive tract, digestive gland diverticula, and gonad follicles). therefore, cg-bpi, whose expression profile and biological properties are reminiscent of mammalian bpis, may provide a first line of defense against potential bacterial invasion. to our knowledge, this is the first characterization of a bpi in an invertebrate.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Grumiaux2007,

title = {Effect of fluidized bed combustion ashes used in metal polluted soil remediation on life history traits of the oligochaeta Eisenia andrei},

author = {Fabien Grumiaux and Sylvain Demuynck and David Schikorski and Sébastien Lemière and Franck Vandenbulcke and Alain Leprêtre},

year  = {2007},

date = {2007-01-01},

journal = {European Journal of Soil Biology},

volume = {43},

number = {SUPPL. 1},

pages = {S256-S260},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Molendi-Coste2007,

title = {Effets d'une dénutrition maternelle périnatale sur la différenciation neuronale et neuroendocrine dans le modèle de la médullosurrénale: des altérations précoces aux implications possibles dans la programmation de pathologies à  l'âge adulte},

author = {Olivier Molendi-Coste and Christine Laborie and Christophe Breton and J Mairesse and Corinne Abbadie and Franck Vandenbulcke and Luca Grumolato and N Aubert and Hafida Ghzili and B Gonzalez and Hubert Vaudry and Youssef Anouar and Didier Vieau},

year  = {2007},

date = {2007-01-01},

booktitle = {34ème colloque de la Société de Neuroendocrinologie, 25-27 septembre 2007, Tours},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Molendi-Coste2007a,

title = {Effects of maternal perinatal undernutrition on the activity of adrenomedullary cells in 8-month-old rats},

author = {Olivier Molendi-Coste and Franck Vandenbulcke and Christophe Breton and Luca Grumolato and Hafida Ghzili and G Van-Camp and Hubert Vaudry and Youssef Anouar and Christine Laborie and Didier Vieau},

year  = {2007},

date = {2007-01-01},

booktitle = {8ème Colloque de la Société des Neurosciences. 22-25 mai 2007, Montpellier},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Munoz2007,

title = {Procedimientos Inmunocitoquímicos para Estudiar la Distribución y la Participación en la Defensa de Péptidos Antimicrobianos},

author = {Marcelo Munoz and Evelyne Bachère and Guillaume Mitta and Annie Desmons and Franck Vandenbulcke},

year  = {2007},

date = {2007-01-01},

journal = {Revista Tecnologica ESPOL},

volume = {6},

pages = {79-87},

abstract = {El presente artículo describe adaptaciones y principios básicos para la realización de análisis inmunocitoquímicospara la detección de péptidos antimicrobianos tanto de camarones como de mejillones. estos análisisinmunocitoquímicos, no solamente describen en forma estática la ubicación de estos péptidos, si no que permitirtener una comprensión funcional de los fenómenos ligados a localización y distribución de estos efectores. losanálisis aquí descritos son variados y comienzan con la descripción de la detección los efectores en corteshistológicos provenientes de bloques de parafina y el uso de la microscopía confocal. además, este artículodescribe también protocolos y pone de manifiesto ciertos detalles técnicos de la detección inmune de los efectoresantes o después de la inclusión en la resina asociados a la microscopía electrónica tanto de células como depequeños piezas de tejidos disectados.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Tirape2007,

title = {Expression of immune-related genes in the oyster Crassostrea gigas during ontogenesis},

author = {Ana Tirapé and Coralie Bacque and Raphaël Brizard and Franck Vandenbulcke and Viviane Boulo},

year  = {2007},

date = {2007-01-01},

journal = {Developmental and Comparative Immunology},

volume = {31},

number = {9},

pages = {859-873},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Brulle2006,

title = {Cloning and real-time PCR testing of 14 potential biomarkers in Eisenia fetida following cadmium exposure},

author = {Franck Brulle and Guillaume Mitta and Claude Cocquerelle and Didier Vieau and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},

year  = {2006},

date = {2006-01-01},

journal = {Environmental Science and Technology},

volume = {40},

number = {8},

pages = {2844-2850},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Brulle2006a,

title = {Cloning and real-time PCR testing of 14 potential biomarkers in Eisenia fetida following cadmium exposure},

author = {Franck Brulle and Guillaume Mitta and Claude Cocquerelle and Didier Vieau and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},

year  = {2006},

date = {2006-01-01},

booktitle = {8th International Symposium on Earthworm Ecology, 4-9 September 2006, Krakow (Pologne)},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Brulle2006b,

title = {Real-time PCR testing of potential biomarkers in soil invertebrates},

author = {Franck Brulle and Guillaume Mitta and Claude Cocquerelle and Didier Vieau and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},

year  = {2006},

date = {2006-01-01},

booktitle = {SETAC Europe 16th Annual Meeting, « Controversies and Solutions in Environmental Sciences » 7-11 May 2006, La Haye (Pays-Bas)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Grumiaux2006,

title = {Effect of fluidized bed combustion ashes used in metal polluted soil remediation on life history traits of the oligochaeta Eisenia andrei},

author = {Fabien Grumiaux and Sylvain Demuynck and Sébastien Lemière and Franck Vandenbulcke and Alain Leprêtre},

year  = {2006},

date = {2006-01-01},

booktitle = {8th International Symposium on Earthworm Ecology, 4-9 September 2006, Krakow (Pologne)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Molendi-Coste2006,

title = {Effets d'une dénutrition maternelle périnatale sur la fonctionnalité des cellules chromaffines de la médullosurrénale chez le rat mâle adulte},

author = {Olivier Molendi-Coste and Franck Vandenbulcke and Christophe Breton and Luca Grumolato and Hafida Ghzili and G Van-Camp and Hubert Vaudry and Youssef Anouar and Christine Laborie and Didier Vieau},

year  = {2006},

date = {2006-01-01},

booktitle = {10ème Journée LARC Neurosciences. 10 novembre 2006, Lille},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Brulle2005,

title = {Identification, characterization and validation of pollution biomarkers in soil invertebrates},

author = {Franck Brulle and Guillaume Mitta and Claude Cocquerelle and Didier Vieau and Sébastien Lemière and Alain Leprêtre and Franck Vandenbulcke},

year  = {2005},

date = {2005-01-01},

booktitle = {Ecotox 2005. Advances and Trends in Ecotoxicology. 5-7 September 2005, Brno (Czech Republic)},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Gonzalez2004,

title = {Molecular characterization of an immune related gene, CgBP/LBP isolated from the oyster Crassostrea gigas},

author = {Marcelo Gonzalez and Yannick Gueguen and Julie Fievet and Franck Vandenbulcke and Evelyne Bachère},

year  = {2004},

date = {2004-01-01},

booktitle = {Aquaculture Europe 2004: Biotechnologies for quality. 20-23 October 2004, Barcelona (Espagne)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Lefebvre2004,

title = {Transcriptomic analysis in the leech Theromyzon tessulatum: Involvement of cystatin B in innate immunity},

author = {Christophe Lefebvre and Claude Cocquerelle and Franck Vandenbulcke and David Hot and Ludovic Huot and Yves Lemoine and Michel Salzet},

year  = {2004},

date = {2004-01-01},

journal = {Biochemical Journal},

volume = {380},

number = {3},

pages = {617-625},

abstract = {At the present time, there is little information on mechanisms of innate immunity in invertebrate groups other than insects, especially annelids. in the present study, we have performed a transcriptomic study of the immune response in the leech theromyzon tessulatum after bacterial challenge, by a combination of differential display rt (reverse transcriptase)–pcr and cdna microarrays. the results show relevant modulations concerning several known and unknown genes. indeed, threonine deaminase, malate dehydrogenase, cystatin b, polyadenylate-binding protein and α-tubulin-like genes are up-regulated after immunostimulation. we focused on cystatin b (stefin b), which is an inhibitor of cysteine proteinases involved in the vertebrate immune response.we have cloned the full-length cdna and named the t. tessulatum gene as tt-cysb. main structural features of cystatins were identified in the derived amino acid sequence of tt-cysb cdna; namely, a glycine residue in the n-terminus and a consensus sequence of gln-xaa-val-xaa-gly (qxvxg) corresponding to the catalytic site. moreover, tt-cysb is the first cystatin b gene characterized in invertebrates. we have determined by in situ hybridization and immunocytochemistry that tt-cysb is only expressed in large coelomic cells. in addition, this analysis confirmed that tt-cysb is up-regulated after bacterial challenge, and that increased expression occurs only in coelomic cells. these data demonstrate that the innate immune response in the leech involves a cysteine proteinase inhibitor that is not found in ecdysozoan models, such as drosophila melanogaster or caenorhabditis elegans, and so underlines the great need for information about innate immunity mechanisms in different invertebrate groups.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Munoz2004,

title = {Involvement of penaeidins in defense reactions of the shrimp Litopenaeus stylirostris to a pathogenic vibrio},

author = {Marcelo Munoz and Franck Vandenbulcke and Julien Garnier and Yannick Gueguen and Philippe Bulet and Denis Saulnier and Evelyne Bachère},

year  = {2004},

date = {2004-01-01},

journal = {Cellular and Molecular Life Sciences},

volume = {61},

number = {7-8},

pages = {961-972},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Riviere2004,

title = {Characterization of the first non-insect invertebrate functional angiotensin-converting enzyme (ACE): Leech TtACE resembles the N-domain of mammalian ACE},

author = {Guillaume Rivière and Annie Michaud and Laurence Deloffre and Franck Vandenbulcke and Angélique Levoye and Christophe Breton and Pierre Corvol and Michel Salzet and Didier Vieau},

year  = {2004},

date = {2004-01-01},

journal = {Biochemical Journal},

volume = {382},

number = {2},

pages = {565-573},

abstract = {Angiotensin-converting enzyme (ace) is a zinc metallopeptidase that plays a major role in blood homoeostasis and reproduction in mammals. in vertebrates, both transmembrane and soluble ace, containing one or two homologous active sites, have been characterized. so far, several aces from invertebrates have been cloned, but only in insects. they are soluble and display a single active site. using biochemical procedures, an ace-like activity was detected in our model, the leech, theromyzon tessulatum. annelida is the most distant phylum in which an ace activity has been observed. to gain more insight into the leech enzyme, we have developed a pcr approach to characterize its mrna. the approx. 2 kb cdna has been predicted to encode a 616-amino-acid soluble enzyme containing a single active site, named ttace (t. tessulatum ace). surprisingly, its primary sequence shows greater similarity to vertebrates than to invertebrates. stable in vitro expression of ttace in transfected chinese-hamster ovary cells revealed that the leech enzyme is a functional metalloprotease. as in mammals, this 79 kda glycosylated enzyme functions as a dipeptidyl carboxypeptidase capable of hydrolysing angiotensin i to angiotensin ii. however, a weak chloride inhibitory effect and acetylated n-acetyl-sdkp (ac sdackp) hydrolysis reveal that ttace activity resembles that of the n-domain of mammalian ace. in situ hybridization shows that its cellular distribution is restricted to epithelial midgut cells. although the precise roles and endogenous substrates of ttace remain to be identified, characterization of this ancestral peptidase will help to clarify its physiological roles in non-insect invertebrate species.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Sautiere2004,

title = {Leech central nervous system response following septic challenge: a proteomic approach},

author = {Pierre-Eric Sautière and David Vergote and Franck Vandenbulcke and Guillaume Mitta and Jacopo Vizioli and Eduardo-R. Macagno and Michel Salzet},

year  = {2004},

date = {2004-01-01},

booktitle = {First international meeting on Evolution, Development and neurobiology of the leech. 29 septembre-3 octobre 2004, Villeneuve d’Ascq},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Tasiemski2004,

title = {Molecular characterization of two novel antibacterial peptides inducible upon bacterial challenge in an annelid, the leech Theromyzon tessulatum},

author = {Aurélie Tasiemski and Franck Vandenbulcke and Guillaume Mitta and Jérôme Lemoine and Christophe Lefebvre and Pierre-Eric Sautière and Michel Salzet},

year  = {2004},

date = {2004-01-01},

journal = {Journal of Biological Chemistry},

volume = {279},

number = {30},

pages = {30973-30982},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Tasiemski2004a,

title = {Molecular characterization of two novel antibacterial peptides inducible upon bacterial challenge in an annelid, the leech Theromyzon tessulatum},

author = {Aurélie Tasiemski and Franck Vandenbulcke and Guillaume Mitta and Jérôme Lemoine and Christophe Lefebvre and Pierre-Eric Sautière and Michel Salzet},

year  = {2004},

date = {2004-01-01},

booktitle = {First international meeting on Evolution, Development and neurobiology of the leech. 29 septembre-3 octobre 2004, Villeneuve d’Ascq, France},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke2004,

title = {Necessity to explore innate immunity in diverse models: the leech Theromyzon tessulatum},

author = {Franck Vandenbulcke},

year  = {2004},

date = {2004-01-01},

booktitle = {XIX International Congress of Zoology. 23-27 August 2004, Beijing (China)},

note = {INV},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Vergote2004,

title = {Up-regulation of neurohemerythrin expression in the central nervous system of the medicinal leech, Hirudo medicinalis, following septic injury},

author = {David Vergote and Pierre-Eric Sautière and Franck Vandenbulcke and Didier Vieau and Guillaume Mitta and Eduardo-R. Macagno and Michel Salzet},

year  = {2004},

date = {2004-01-01},

journal = {Journal of Biological Chemistry},

volume = {279},

number = {42},

pages = {43828-43837},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Bachere2003,

title = {Penaeidins, a family of antimicrobial peptides ubiquitous in Crustacea: involvement in shrimp immune response to infections},

author = {Evelyne Bachère and Delphine Destoumieux-Garzón and Marcelo Munoz and Franck Vandenbulcke and Philippe Bulet},

year  = {2003},

date = {2003-01-01},

booktitle = {4th International Gordon Research Conference on Antimicrobial Peptides, 27 April-2 May 2003, Barga (Italie)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Bachere2003a,

title = {Penaeidines, une famille de peptides antimicrobiens: implication dans la réponse immunitaire},

author = {Evelyne Bachère and Marcelo Munoz and Franck Vandenbulcke and Philippe Bulet and Julien Garnier and Julie Fievet and Jacques Dietrich and Bernard Romestand and Yannick Gueguen},

year  = {2003},

date = {2003-01-01},

booktitle = {Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Gonzalez2003,

title = {Identification de la première protéine de type LBP/BPI chez un invertébré, l’huitre creuse Crassotrea gigas: étude de l’expression du gène et caractérisation préliminaire de la protéine recombinante},

author = {Marcelo Gonzalez and Yannick Gueguen and Jean-Michel Escoubas and Jacques Dietrich and Julie Fievet and Franck Vandenbulcke and Evelyne Bachère},

year  = {2003},

date = {2003-01-01},

booktitle = {Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Lefebvre2003,

title = {Implication de la cystatine B et des cathepsines dans l'immunité innée chez la sangsue Theromyzon tessulatum},

author = {Christophe Lefebvre and Claude Cocquerelle and Franck Vandenbulcke and David Hot and Ludovic Huot and Yves Lemoine and Michel Salzet},

year  = {2003},

date = {2003-01-01},

booktitle = {Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Munoz2003,

title = {Expression of penaeidin antimicrobial peptides in early larval stages of the shrimp Penaeus vannamei},

author = {Marcelo Munoz and Franck Vandenbulcke and Yannick Gueguen and Evelyne Bachère},

year  = {2003},

date = {2003-01-01},

journal = {Developmental and Comparative Immunology},

volume = {27},

number = {4},

pages = {283-289},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Roch2003,

title = {Originality of the Mytilus (Bivalve Mollusc) antibacterial peptides: structurally related to insects but involved as in mammals},

author = {Philippe Roch and Guillaume Mitta and Franck Vandenbulcke and Michel Salzet and André Aumelas and Yin-Shan Yang and Alain Chavanieu and Bernard Calas},

year  = {2003},

date = {2003-01-01},

booktitle = {The new panorama of animal evolution. Proceedings of the XVIII International Congress of Zoology, September 2000, Athens (Greece)},

pages = {177-184},

publisher = {Pensoft Publishers. Sofia-Moscow},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vergote2003,

title = {Le système nerveux de la sangsue répond il à une stimulation bactérienne?},

author = {David Vergote and Franck Vandenbulcke and Didier Vieau and Michel Salzet and Pierre-Eric Sautière},

year  = {2003},

date = {2003-01-01},

booktitle = {Réunion Immunologie des Invertébrés. 17-19 novembre 2003, Banyuls},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@book{Beaudet2002,

title = {Confocal and Electron Microscopic Tracking of Internalized Neuropeptides and/or Their Receptors},

author = {Alain Beaudet and Alexander-C. Jackson and Franck Vandenbulcke},

year  = {2002},

date = {2002-01-01},

booktitle = {Cellular and Molecular Methods in Neuroscience Research},

pages = {15-28},

abstract = {This chapter describes confocal and electron microscopic methods for tracking peptide ligands and/or their receptors following their internalization in cell cultures or brain slices. the confocal microscopic techniques are based upon the use of high affinity fluorescent ligands that were originally developed in our laboratory to study the fate of internalized neurotensin (nt), somatostatin (srif), and opioid peptides (9,13,17). the electron microscopic techniques are adapted from the pre-embedding immunogold method developed by virginia pickel and her team (5) as applied by us to study the effect of ligand exposure on the subcellular distribution of various subtypes of neuropeptide receptors.},

note = {OS},

keywords = {ER4},

pubstate = {published},

tppubtype = {book}

}

@article{Munoz2002,

title = {Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp},

author = {Marcelo Munoz and Franck Vandenbulcke and Denis Saulnier and Evelyne Bachère},

year  = {2002},

date = {2002-01-01},

journal = {European Journal of Biochemistry},

volume = {269},

number = {11},

pages = {2678-2689},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@book{Sautiere2002,

title = {New powerful cascade coagulant inhibitors isolated from the gut of the leech Theromyzon tessulatum},

author = {Pierre-Eric Sautière and Aurélie Tasiemski and Franck Vandenbulcke and Annie Desmons and Michel Salzet},

year  = {2002},

date = {2002-01-01},

booktitle = {A new model for analyzing antimicrobial peptides with biomedical applications},

edition = {E.L. Cooper et al. (Eds) IOS Press},

note = {OS},

keywords = {ER4},

pubstate = {published},

tppubtype = {book}

}

@article{Tasiemski2002,

title = {Presence of chromogranin-derived antimicrobial peptides in plasma during coronary artery bypass surgery and evidence of an immune origin of these peptides},

author = {Aurélie Tasiemski and Hamida Hammad and Franck Vandenbulcke and Christophe Breton and Thomas-J. Bilfinger and Joel Pestel and Michel Salzet},

year  = {2002},

date = {2002-01-01},

journal = {Blood},

volume = {100},

number = {2},

pages = {553-559},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Vandenbulcke2002,

title = {Autometallography: a cytological method for intracellular localization of heavy metals},

author = {Franck Vandenbulcke and Claire Grelle and Marie-Chantal Fabre and Michel Descamps},

year  = {2002},

date = {2002-01-01},

booktitle = {Advantages and limits. Joint Microscopy Meeting. 25-28 juin 2002, Lille},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke2002a,

title = {Caractérisation et rôle des peptides antimicrobiens dans l’immunité innée de la sangsue Theromyzon tessulatum},

author = {Franck Vandenbulcke and Aurélie Tasiemski and H Finet and Annie Desmons and Jacopo Vizioli and Pierre-Eric Sautière and Michel Salzet},

year  = {2002},

date = {2002-01-01},

booktitle = {126ème Congrés de la Société Zoologique de France. 16-18 septembre 2002, Plouzané},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Deloffre2001,

title = {Caractérisation moléculaire d’une molécule apparentée à la progranuline, chez Hediste diversicolor},

author = {Laurence Deloffre and Pierre-Eric Sautière and Didier Vieau and Franck Vandenbulcke and Michel Salzet and Jean-Claude Andriès},

year  = {2001},

date = {2001-01-01},

booktitle = {Colloque « Immunologie des Invertébrés ». 11-14 Septembre 2001, Villeneuve d’Ascq},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Matias2001,

title = {Evidence for an endocannabinoid system in the central nervous system of the leech Hirudo medicinalis},

author = {Isabel Matias and Tiziana Bisogno and Dominique Melck and Franck Vandenbulcke and Martine Verger-Bocquet and Luciano De-Petrocellis and Christian Sergheraert and Christophe Breton and Vicenzo Di-Marzo and Michel Salzet},

year  = {2001},

date = {2001-01-01},

journal = {Molecular Brain Research},

volume = {87},

number = {2},

pages = {145-159},

abstract = {In invertebrates, like hydra and sea urchins, evidence for a functional cannabinoid system was described. the partial characterization of a putative cb1 cannabinoid receptor in the leech hirudo medicinalis led us to investigate the presence of a complete endogenous cannabinoid system in this organism. by using gas chromatography–mass spectrometry, we demonstrate the presence of the endocannabinoids anandamide (n-arachidonoylethanolamine, 21.560.7 pmol/g) and 2-arachidonoyl-glycerol (147.4642.7 pmol/g), and of the biosynthetic precursor of anandamide, n-arachidonylphosphatidyl-ethanolamine (16.563.3 pmol/g), in the leech central nervous system (cns). anandamide-related molecules such as n-palmitoylethanolamine (32.461.6 pmol/g) and n-linolenoylethanolamine (5.8 pmol/g) were also detected.we also found an anandamide amidase activity in the leech cns cytosolic fraction with a maximal activity at ph 7 and little sensitivity to typical fatty acid amide hydrolase (faah) inhibitors. using an antiserum directed against the amidase signature sequence, we focused on the identification and the localization of the leech amidase. firstly, leech nervous system protein extract was subjected to western blot analysis, which showed three immunoreactive bands at ca. |42, |46 and |66 kda. the former and latter bands were very faint and were also detected in whole homogenates from the coelenterate hydra vulgaris, where the presence of cb1-like receptors, endocannabinoids and a faah-like activity was reported previously. secondly, amidase immunocytochemical detection revealed numerous immunoreactive neurons in the cns of three species of leeches. in addition, we observed that leech amidase-like immunoreactivity matches to a certain extent with cb1-like immunoreactivity. finally, we also found that stimulation by anandamide of this receptor leads, as in mammals, to inhibition of camp formation, although this effect appeared to be occurring through the previously described anandamide-induced and cb1-mediated activation of nitric oxide release. taken together, these results suggest the existence of a complete and functional cannabinoid system in leeches. ó 2001 elsevier science b.v. all rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Munoz2001,

title = {Les pénaeidines dans la réponse immunitaire chez les crevettes pénéides},

author = {Marcelo Munoz and Franck Vandenbulcke and Denis Saulnier and Evelyne Bachère},

year  = {2001},

date = {2001-01-01},

booktitle = {Colloque « Immunologie des Invertébrés ». 11-14 Septembre 2001, Villeneuve d’Ascq},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Riviere2001,

title = {Clonage et étude du mode d’expression de l’enzyme de conversion de l’angiotensine chez la sangsue Theromyzon tessulatum},

author = {Guillaume Rivière and Laurence Deloffre and Franck Vandenbulcke and Angélique Levoye and Christophe Breton and Michel Salzet and Didier Vieau},

year  = {2001},

date = {2001-01-01},

booktitle = {Colloque du réseau LARC (Lille-Amiens-Rouen-Caen). 15 octobre 2001, Rouen},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Tasiemski2001,

title = {Gene expression of theromacin, a novel cystein rich antibacterial peptide isolated from the leech Theromyzon tessulatum, is induced after bacterial challenge},

author = {Aurélie Tasiemski and Franck Vandenbulcke and Guillaume Mitta and Pierre-Eric Sautière and Jérôme Lemoine and Michel Salzet},

year  = {2001},

date = {2001-01-01},

booktitle = {Colloque « Immunologie des Invertébrés ». 11-14 septembre 2001, Villeneuve d’Ascq},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Chopin2000,

title = {Therostasin, a novel clotting factor Xa inhibitor from the rhynchobdellid leech, Theromyzon tessulatum},

author = {Vincent Chopin and Michel Salzet and Jean-Luc Baert and Franck Vandenbulcke and Pierre-Eric Sautière and Jean-Pierre Kerckaert and Jean Malecha},

year  = {2000},

date = {2000-01-01},

journal = {Journal of Biological Chemistry},

volume = {275},

number = {42},

pages = {32701-32707},

abstract = {Therostasin is a potent naturally occurring tight-binding inhibitor of mammalian factor xa (k i, 34 pm), isolated from the rhynchobdellid leechtheromyzon tessulatum. therostasin is a cysteine-rich protein (8991 da) consisting of 82 amino acid residues with 16 cysteine residues. its amino acid sequence has been determined by a combination of techniques, including edman degradation, enzymatic cleavage, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) on the native and s-β-pyridylethylated compound. sequence analysis reveals that it shares no significant homology with other factor xa inhibitors except for the putative reactive site. moreover, it contains a signature pattern for proteins of the endothelin family, potent vasoconstrictors isolated in mammal and snake venom. therostasin cdna (825 bp) codes for a polypeptide of 82 amino acid residues preceded by 19 residues, representing a signal peptide sequence. as for the other known inhibitors of factor xa, therostasin is expressed and stored in the cells of the leech salivary glands.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Matias2000,

title = {Evidence for a cannabinoid system in leech Hirudo medicinalis},

author = {Isabel Matias and Tiziana Bisogno and Dominique Melck and Franck Vandenbulcke and Christophe Breton and Vicenzo Di-Marzo and Michel Salzet},

year  = {2000},

date = {2000-01-01},

booktitle = {International Symposium on non-mammalian eicosanoids and bioactive lipids. 29-31 May 2000, Berlin (Allemagne)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Mitta2000,

title = {Involvement of mytilins in mussel antimicrobial defense},

author = {Guillaume Mitta and Franck Vandenbulcke and Florence Hubert and Michel Salzet and Philippe Roch},

year  = {2000},

date = {2000-01-01},

journal = {Journal of Biological Chemistry},

volume = {275},

number = {17},

pages = {12954-12962},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Mitta2000a,

title = {Differential distribution and defence involvement of antimicrobial peptides in mussel},

author = {Guillaume Mitta and Franck Vandenbulcke and Thierry Noël and Bernard Romestand and Jean-Claude Beauvillain and Michel Salzet and Philippe Roch},

year  = {2000},

date = {2000-01-01},

journal = {Journal of Cell Science},

volume = {113},

number = {15},

pages = {2759-2769},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Mitta2000b,

title = {Original involvement of antimicrobial peptides in mussel innate immunity},

author = {Guillaume Mitta and Franck Vandenbulcke and Philippe Roch},

year  = {2000},

date = {2000-01-01},

journal = {FEBS Letters},

volume = {486},

number = {3},

pages = {185-190},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Vandenbulcke2000,

title = {Ligand-induced internalization of neurotensin in transfected COS-7 cells: Differential intracellular trafficking of ligand and receptor},

author = {Franck Vandenbulcke and Dominique Nouel and Jean-Pierre Vincent and Jean Mazella and Alain Beaudet},

year  = {2000},

date = {2000-01-01},

journal = {Journal of Cell Science},

volume = {113},

number = {17},

pages = {2963-2975},

abstract = {The neuropeptide neurotensin (nt) is known to be internalized in a receptor-mediated fashion into its target cells. to gain insight into the mechanisms underlying this process, we monitored in parallel the migration of the nt1 neurotensin receptor subtype and a fluorescent analog of nt (fluo-nt) in cos-7 cells transfected with a tagged nt1 construct. fluo-nt internalization was prevented by hypertonic sucrose, potassium depletion and cytosol acidification, demonstrating that it proceeded via clathrin-coated pits. within 0–30 minutes, fluo-nt accumulated together with its receptor in acridine orange-positive, acidic organelles. these organelles concentrated transferrin and immunostained positively for rab 5a, therefore they were early endosomes. after 30–45 minutes, the ligand and its receptor no longer colocalized. fluo-nt was first found in rab 7-positive late endosomes and later in a nonacidic juxtanuclear compartment identified as the trans-golgi network (tgn) by virtue of its staining for syntaxin 6. this juxtanuclear compartment also stained positively for rab 7 and for the tgn/pericentriolar recycling endosome marker rab 11, suggesting that the ligand could have been recruited to the tgn from either late or recycling endosomes. by that time, internalized receptors were detected in lamp-1-immunoreactive lysosomes. these results demonstrate that neurotensin/nt1 receptor complexes follow a recycling cycle that is unique among the g protein-coupled receptors studied to date, and provide the first evidence for the targeting of a nonendogenous protein from endosomes to the tgn},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Deram1999,

title = {Lead biodisponibility to soil invertebrate fauna and perennial grass},

author = {Annabelle Deram and Claire Grelle and Franck Vandenbulcke and Marie-Chantal Fabre and Patrick Scaps and Michel Descamps},

year  = {1999},

date = {1999-01-01},

booktitle = {International Conference on lead exposure, Reproductive toxixity and carcinogenicity. 7-9 June 1999, Gargano (Italie)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Garrido1999,

title = {Confocal microscopy reveals thimet oligopeptidase (EC 3.4.24.15) and neurolysin (EC 3.4.24.16) in the classical secretory pathway},

author = {Paula-A.G. Garrido and Franck Vandenbulcke and Antoine-R. Ramjaun and Bruno Vincent and Frédéric Checler and Emer Ferro and Alain Beaudet},

year  = {1999},

date = {1999-01-01},

journal = {DNA and Cell Biology},

volume = {18},

number = {4},

pages = {323-331},

abstract = {Thimet oligopeptidase (ec 3.4.24.15; ep24.15) and neurolysin (ec 3.4.24.16; ep24.16) are closely related enzymes involved in the metabolic inactivation of bioactive peptides. both of these enzymes were previously shown to be secreted from a variety of cell types, although their primary sequence lacks a signal peptide. to investigate the mechanisms responsible for this secretion, we examined by confocal microscopy the subcellular localization of these two enzymes in the neuroendocrine cell line att20. both ep24.15 and ep24.16 were found by immunohistochemistry to be abundantly expressed in att20 cells. western blotting experiments confirmed that the immunoreactivity detected in the soma of these cells corresponded to previously cloned isoforms of the enzymes. at the subcellular level, both enzymes colocalized extensively with the integral trans-golgi network protein, syntaxin-6, in the juxtanuclear region. in addition, both ep24.15 and ep24.16 were found within small vesicular organelles distributed throughout the cell body. some, but not all, of these organelles also stained positively for acth. these results demonstrate that both ep24.15 and ep24.16 are present within the classical secretory pathway. their colocalization with acth further suggests that they may be targeted to the regulated secretory pathway, even in the absence of a signal peptide.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Grelle1999,

title = {L’impact des métaux lourds sur les peuplements de macroinvertébrés de la faune du sol},

author = {Claire Grelle and Marie-Chantal Fabre and Alain Leprêtre and Franck Vandenbulcke and Michel Descamps},

year  = {1999},

date = {1999-01-01},

booktitle = {Colloque « Les enjeux, pour la santé humaine et le maintien de la biodiversité, de la caractérisation et de la validation de systèmes d’évaluation écotoxicologiques de la qualité des sédiments et des boues de dragage. MACC. 28 septembre 1999, Villeneuve d’Ascq},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Matias1999,

title = {L’anandamide amidase chez les Hirudinées},

author = {Isabel Matias and Christophe Breton and Franck Vandenbulcke and Martine Verger and Michel Salzet},

year  = {1999},

date = {1999-01-01},

booktitle = {Journée Scientifique du réseau LARC-Neurosciences. 3 octobre 1999, Amiens},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Matias1999a,

title = {L’anandamide amidase chez les Hirudinées},

author = {Isabel Matias and Christophe Breton and Franck Vandenbulcke and Martine Verger and Michel Salzet},

year  = {1999},

date = {1999-01-01},

booktitle = {Réunion « Immunologie de Invertébrés ». 19-22 septembre 1999, Mont Sainte-Odile},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Mitta1999,

title = {Mussel defensins are synthesised and processed in granulocytes then released into the plasma after bacterial challenge},

author = {Guillaume Mitta and Franck Vandenbulcke and Florence Hubert and Philippe Roch},

year  = {1999},

date = {1999-01-01},

journal = {Journal of Cell Science},

volume = {112},

number = {23},

pages = {4233-4242},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Vandenbulcke1999,

title = {Les peptides antimicrobiens chez Mytilus galloprovincialis},

author = {Franck Vandenbulcke and Guillaume Mitta and Michel Salzet and Philippe Roch},

year  = {1999},

date = {1999-01-01},

booktitle = {Réunion « Immunologie de Invertébrés ». 19-22 septembre 1999, Mont Sainte-Odile},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke1999a,

title = {Localisation ultrastructurale des peptides antimicrobiens Mytilin et MGD1 chez Mytilus},

author = {Franck Vandenbulcke and Guillaume Mitta and Michel Salzet and Philippe Roch},

year  = {1999},

date = {1999-01-01},

booktitle = {Journée Scientifique du réseau LARC-Neurosciences. 3 octobre 1999, Amiens},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Beaudet1998,

title = {Fluorescent ligands for studying neuropeptide receptors by confocal microscopy},

author = {Alain Beaudet and Dominique Nouel and Thomas Stroh and Franck Vandenbulcke and C Dal-Farra and Jean-Pierre Vincent},

year  = {1998},

date = {1998-01-01},

journal = {Brazilian Journal of Medical and Biological Research},

volume = {31},

number = {11},

pages = {1479-1489},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Garrido1998,

title = {Metalloendopeptidase EC 3.4.24.15 is targeted to and secreted from the regulated secretory pathway in att-20 cells},

author = {Paula-A.G. Garrido and Franck Vandenbulcke and Emer-Suavinho Ferro and Alain Beaudet},

year  = {1998},

date = {1998-01-01},

booktitle = {Congrès de la Fédération des Sociétés de Biologie Expérimentale. 26-29 August 1998, Caxambu (Brésil)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Valentijn1998,

title = {Distribution, cellular localization, and ontogeny of preprothyrotropin- releasing hormone-(160-169) (Ps4)-binding sites in the rat pituitary},

author = {Karine Valentijn and Franck Vandenbulcke and Ester Piek and Jean-Claude Beauvillain and Hubert Vaudry},

year  = {1998},

date = {1998-01-01},

journal = {Endocrinology},

volume = {139},

number = {3},

pages = {1306-1313},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Vandenbulcke1998,

title = {Implication of the midgut of the centipede Lithobius forficatus in the heavy metal detoxification process},

author = {Franck Vandenbulcke and Claire Grelle and Marie-Chantal Fabre and Michel Descamps},

year  = {1998},

date = {1998-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {41},

number = {3},

pages = {258-268},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Vandenbulcke1998a,

title = {Ultrastructural and autometallographic studies of the nephrocytes of Lithobius forficatus L. (Myriapoda, Chilopoda): Role in detoxification of cadmium and lead},

author = {Franck Vandenbulcke and Claire Grelle and Marie-Chantal Fabre and Michel Descamps},

year  = {1998},

date = {1998-01-01},

journal = {International Journal of Insect Morphology and Embryology},

volume = {27},

number = {2},

pages = {111-120},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Vandenbulcke1998b,

title = {Cheminement intracellulaire differentiel de la neurotensine et de son récepteur après internalisation dans des cellules COS-7 transfectées},

author = {Franck Vandenbulcke and Dominique Nouel and Jean-Pierre Vincent and Alain Beaudet},

year  = {1998},

date = {1998-01-01},

booktitle = {XXVII colloque de la Société de Neuroendocrinologie Expérimentale. 2-5 septembre 1998, Lille},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke1998c,

title = {Differential intracellular trafficking of neurotensin and of its receptor in COS-7 transfected cells},

author = {Franck Vandenbulcke and Dominique Nouel and Jean-Pierre Vincent and Alain Beaudet},

year  = {1998},

date = {1998-01-01},

booktitle = {28th annual meeting of the Society for Neuroscience. 7-12 November 1998, Los Angeles (USA)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Deloffre1997,

title = {Caractérisation biochimique d’une molécule apparentée aux granulines mammaliennes chez un Annélide Polychète, Nereis diversicolor},

author = {Laurence Deloffre and Pierre-Eric Sautière and Franck Vandenbulcke and Jean-Claude Andriès},

year  = {1997},

date = {1997-01-01},

booktitle = {Première Journée Scientifique du réseau LARC-Neurosciences. 3 octobre 1997, Rouen},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Grelle1997,

title = {Effets des métaux lourds sur l'ultrastructure d'un organe athrocytaire chez Lithobius forficatus L. (Myriapode Chilopode)},

author = {Claire Grelle and Marie-Chantal Fabre and Franck Vandenbulcke and Michel Descamps},

year  = {1997},

date = {1997-01-01},

booktitle = {Colloque Lille-ULB. 9 Janvier 1997, Bruxelles (Belgique)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Salzet1997,

title = {Leech egg-laying-like hormone: Structure, neuronal distribution and phylogeny},

author = {Michel Salzet and Martine Verger-Bocquet and Franck Vandenbulcke and Jan Van-Minnen},

year  = {1997},

date = {1997-01-01},

journal = {Molecular Brain Research},

volume = {49},

number = {1-2},

pages = {211-221},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Vandenbulcke1997a,

title = {L’autométallographie: une méthode pour la localisation intracellulaire des métaux lourds. Application à un invertébré du sol, le mille-pattes Lithobius forficatus (Myriapode Chilopode)},

author = {Franck Vandenbulcke and Claire Grelle and Marie-Chantal Fabre and Michel Descamps},

year  = {1997},

date = {1997-01-01},

booktitle = {Congrès “ L’après charbon ”. 17-20 mars 1997, Lille},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Vandenbulcke1997b,

title = {Biochemical identification and ganglionic localization of leech angiotensin-converting enzymes},

author = {Franck Vandenbulcke and Virginie Laurent and Martine Verger-Bocquet and George-B. Stefano and Michel Salzet},

year  = {1997},

date = {1997-01-01},

journal = {Molecular Brain Research},

volume = {49},

number = {1-2},

pages = {229-237},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Vandenbulcke1997bb,

title = {Distribution, localisation cellulaire et ontogenene des sites de liaison du PS4 (pré pro TRH 160-169) dans l'hypophyse du rat},

author = {Franck Vandenbulcke and Karine Valentijn and Ester Piek and Jean-Claude Beauvillain and Hubert Vaudry},

year  = {1997},

date = {1997-01-01},

booktitle = {Première Journée Scientifique du réseau LARC-Neurosciences. 3 octobre 1997, Rouen},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Laurent1996,

title = {The leech Angiotensin-Converting Enzyme},

author = {Virginie Laurent and Franck Vandenbulcke and Martine Verger-Bocquet and Michel Salzet},

year  = {1996},

date = {1996-01-01},

booktitle = {2nd Meeting of European Neuroscience. 24-28 septembre 1996, Strasbourg},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Laurent1996a,

title = {The leech angiotensin-converting enzyme},

author = {Virginie Laurent and Franck Vandenbulcke and Martine Verger-Bocquet and Michel Salzet},

year  = {1996},

date = {1996-01-01},

booktitle = {Congress of Comparative Endocrinology. 10-14 septembre 1996, Rouen},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Salzet1996,

title = {Structural characterization of osmoregulator peptides from the brain of the leech Theromyzon tessulatum: IPEPYVWD and IPEPYVWD-amide},

author = {Michel Salzet and Franck Vandenbulcke and Martine Verger-Bocquet},

year  = {1996},

date = {1996-01-01},

journal = {Molecular Brain Research},

volume = {43},

number = {1-2},

pages = {301-310},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Vandenbulcke1996,

title = {Localisation ultrastructurale de l'endopeptidase-24.11 dans l'éminence médiane du Rat: arguments morphologiques en faveur d'une action locale modulatrice des enképhalines},

author = {Franck Vandenbulcke},

year  = {1996},

date = {1996-01-01},

booktitle = {Conférence dans le cadre du Centre de Biologie Cellulaire (Unité de neuroendocrinologie expérimentale) de l'Université de Lille I. 15 février 1996, Villeneuve d’Ascq},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Ciofi1995,

title = {Immunoréactivité anti-récepteur mu dans l'éminence médiane du rat: distribution et plasticité d'expression},

author = {Philippe Ciofi and Franck Vandenbulcke and Soizic Dutoit and D Deneux and A Mansour and S J Watson and G Tramu and Jean-Claude Beauvillain},

year  = {1995},

date = {1995-01-01},

booktitle = {24ème Colloque de la Société de Neuroendocrinologie Expérimentale. 19-22 septembre 1995, Québec (Canada)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Ciofi1995a,

title = {Mu opioid receptor-like immunoreactivity in the rat median eminence: distribution and plasticity in expression},

author = {Philippe Ciofi and Franck Vandenbulcke and Soizic Dutoit and D Deneux and A Mansour and S J Watson and G Tramu and Jean-Claude Beauvillain},

year  = {1995},

date = {1995-01-01},

booktitle = {Society for Neuroscience. 11-16 November 1995, San Diego, California (USA)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke1995,

title = {Nature des systèmes neurohormonaux contenant l'endopeptidase neutre dans l'éminence médiane du Rat: étude ultrastructurale},

author = {Franck Vandenbulcke and Philippe Ciofi and G Tramu and Soizic Dutoit and Jean-Claude Beauvillain},

year  = {1995},

date = {1995-01-01},

booktitle = {24ème Colloque de la Société de Neuroendocrinologie Expérimentale. 19-22 septembre 1995, Québec (Canada)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Vandenbulcke1994,

title = {Concomitant variation in immunoreactivity for enkephalins and neutral endopeptidase in the median eminence of the lactating rat},

author = {Franck Vandenbulcke and Philippe Ciofi and Jean-Claude Beauvillain},

year  = {1994},

date = {1994-01-01},

journal = {Neuroscience Letters},

volume = {175},

number = {1-2},

pages = {53-57},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Vandenbulcke1994a,

title = {Detection of neutral endopeptidase (NEP, Enkephalinase, E.C.3.4.24.11) in relation to dopaminergic and gonadoliberinergic nerve endings in the median eminence of the male rat: A double labeling ultrastructural study},

author = {Franck Vandenbulcke and Philippe Ciofi and Jean-Claude Beauvillain},

year  = {1994},

date = {1994-01-01},

journal = {Journal of Neuroendocrinology},

volume = {6},

number = {6},

pages = {655-664},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Vandenbulcke1994b,

title = {Concomitant variation in immunoreactivity for enkephalins and neutral endopeptidase in the median eminence of lactating rats},

author = {Franck Vandenbulcke and Philippe Ciofi and Jean-Claude Beauvillain},

year  = {1994},

date = {1994-01-01},

booktitle = {Society for Neuroscience. 13-18 November 1994, Miami Beach, Florida (USA)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke1994c,

title = {Variation concomitante d'immunoréactivité pour l'endopeptidase neutre et les enképhalines dans l'éminence médiane du Rat pendant la lactation},

author = {Franck Vandenbulcke and Philippe Ciofi and Jean-Claude Beauvillain},

year  = {1994},

date = {1994-01-01},

booktitle = {23° Colloque de la société de Neuroendocrinologie Expérimentale. 1-3 septembre 1994, Sophia Antipolis},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke1993a,

title = {Modulation enképhalinergique indirecte de la libération de GnRH dans l'éminence médiane du Rat: arguments morphologiques},

author = {Franck Vandenbulcke and Philippe Ciofi and D Deneux and Jean-Claude Beauvillain},

year  = {1993},

date = {1993-01-01},

booktitle = {22ème Colloque de la Société de Neuroendocrinologie Expérimentale. 8-10 Septembre 1993, Leuven/Louvain (Belgique)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke1993b,

title = {Enkephalins modulate median eminence GnRH release through a dopaminergic mechanism: morphological evidence},

author = {Franck Vandenbulcke and Philippe Ciofi and I Dutriez and D Deneux and Jean-Claude Beauvillain},

year  = {1993},

date = {1993-01-01},

booktitle = {Society for Neuroscience. 7-12 November 1993, Washington (USA)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Vandenbulcke1993c,

title = {Arguments morphologiques ultrastructuraux en faveur de l'existence d'une modulation de type paracrine dans l'éminence médiane du Rat},

author = {Franck Vandenbulcke and D Deneux and Valérie Mitchell and Jean-Claude Beauvillain},

year  = {1993},

date = {1993-01-01},

booktitle = {75e Congrès de l'Association des Anatomistes. 12-14 mai 1993, Lille},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Vandenbulcke1993d,

title = {Radioimmunocytochemical distribution of neutral endopeptidase (enkephalinase E.C.3.4.24.11) at the ultrastructural level in the rat median eminence},

author = {Franck Vandenbulcke and Hélène Pollard and Valérie Mitchell and Jean-Claude Beauvillain},

year  = {1993},

date = {1993-01-01},

journal = {Journal of Neuroendocrinology},

volume = {5},

number = {2},

pages = {205-212},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Beauvillain1992,

title = {Arguments morphologiques ultrastructuraux en faveur d'une action des enképhalines sur des prolongements tanycytaires dans l'éminence médiane},

author = {Jean-Claude Beauvillain and Franck Vandenbulcke and Valérie Mitchell and P Poulain and Hélène Pollard and E Moyse and Muriel Mazzuca},

year  = {1992},

date = {1992-01-01},

booktitle = {Colloque de la Société des Neurosciences. 4-7 mai 1992, Strasbourg},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}