Membre

@article{hechelski:hal-04633986,

title = {Influence of P-fertilizers on photosynthetic pigments and phytohormones of perennial ryegrass grown on co-contaminated soils},

author = {Marie Hechelski and Brice Louvel and Christophe Waterlot},

url = {https://hal.science/hal-04633986},

doi = {10.1007/s10725-023-01088-9},

year  = {2023},

date = {2023-11-01},

urldate = {2023-11-01},

journal = {Plant Growth Regulation},

volume = {102},

number = {3},

pages = {659-668},

publisher = {Springer Verlag},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{leclercqdransart:hal-03767130,

title = {Comparison of the interest of four types of organic mulches to reclaim degraded areas (Part 2): Microbial activities and abiotic factors},

author = {Julie Leclercq-Dransart and Sylvain Demuynck and Fabien Grumiaux and Alain Leprêtre and Sébastien Lemière and Brice Louvel and Céline Pernin and Francis Douay},

url = {https://hal.archives-ouvertes.fr/hal-03767130},

doi = {10.1016/j.ecoleng.2022.106694},

year  = {2022},

date = {2022-09-01},

urldate = {2022-09-01},

journal = {Ecological Engineering},

volume = {182},

pages = {106694},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Mikajlo2022,

title = {Plant-soil nitrogen, carbon and phosphorus content after the addition 

	of biochar, bacterial inoculums and nitrogen fertilizer},

author = {Irina Mikajlo and Bertrand Pourrut and Brice Louvel and Jaroslav Hynst and Jaroslav Zahora},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Plant Nutrition},

pages = {1-15},

abstract = {The use of biochar in combination with mineral or biological amendments to improve its influence on soil-plant properties has received growing attention. The changes of nitrogen, carbon and phosphorus content in Lactuca sativa var. capitata aboveground plant biomass and soil after the addition of beech wood biochar combined with the addition of bacterial inoculums (Bacofil and Novarefm) and nitrogen fertilizer have been studied using spectrophotometry methods. Pots were filled with the arable soil from the plots in the protection zone of water sources (Březová nad Svitavou, South Moravia, Czech Republic). Biochar with inoculums decreased plant growth in the first yield of Novaferm treatment and both yields of Bactofil treatment. Increased plant biomass growth was observed with Novaferm addition in the second yield. Total nitrogen increase has been obtained in the plant aboveground biomass and soil of the treatments amended with inoculums and nitrogen fertilizer. The decrease of phosphorus content has been observed in plant aboveground biomass in the biochar amended samples.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Hechelski2021,

title = {Biomass of ryegrass from field experiments: toward a cost-effective and efficient biosourced catalyst for the synthesis of Moclobemide},

author = {Marie Hechelski and Christophe Waterlot and Pierrick Dufrénoy and Brice Louvel and Adam Daïch and Alina Ghinet},

year  = {2021},

date = {2021-01-01},

journal = {Green Chemistry Letters and Reviews},

volume = {14},

number = {1},

pages = {13-20},

abstract = {Lolium perenne L., a common plant perennial ryegrass from Europe, 

	northern Africa and Asia, was selected to produce biomass under field 

	conditions. The biomass of this nonfood crop was from a highly contaminated 

	agricultural soil by potentially toxic metals (Cd, Pb and Zn) with 

	the aim of maintaining the agricultural vocation of these soils. 

	In this perspective and in view of the metal concentration (Cd, Pb, 

	Zn, macro- and micro-nutrients), the biomass of ryegrass was considered 

	as a bio ’ore’ resource and was used as the starting material for 

	the preparation of contemporary biosourced catalysts. The heterogeneous 

	catalyst from the current work was successfully used in the synthesis 

	of Moclobemide, used to treat anxiety and major depressive episodes. 

	Through this original approach, Moclobemide was synthesized in one 

	step, in 80% yield under solvent-free conditions.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{atmos12080994,

title = {Experimental Comparative Study between Conventional and Green Parking Lots: Analysis of Subsurface Thermal Behavior under Warm and Dry Summer Conditions},

author = {Ryad Bouzouidja and François Leconte and Márton Kiss and Margaux Pierret and Christelle Pruvot and Sébastien Détriché and Brice Louvel and Julie Bertout and Zakaria Aketouane and Tingting Vogt Wu and Rémy Goiffon and Baptiste Colin and Anélie Pétrissans and Philippe Lagière and Mathieu Pétrissans},

url = {https://www.mdpi.com/2073-4433/12/8/994},

doi = {10.3390/atmos12080994},

issn = {2073-4433},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Atmosphere},

volume = {12},

number = {8},

abstract = {Green infrastructure has a role to play in climate change adaptation strategies in cities. Alternative urban spaces should be designed considering new requirements in terms of urban microclimate and thermal comfort. Pervious pavements such as green parking lots can contribute to this goal through solar evaporative cooling. However, the cooling benefits of such systems remain under debate during dry and warm periods. The aim of this study was to compare experimentally the thermal behavior of different parking lot types (PLTs) with vegetated urban soil. Four parking lots were instrumented, with temperature probes buried at different depths. Underground temperatures were measured during summer 2019, and the hottest days of the period were analyzed. Results show that the less mineral used in the surface coating, the less it warms up. The temperature difference at the upper layer can reach 10 °C between mineral and non-mineral PLTs. PLTs can be grouped into three types: (i) high surface temperature during daytime and nighttime, important heat transfer toward the sublayers, and low time shift (asphalt system); (ii) high (resp. low) surface temperature during daytime (resp. nighttime), weak heat transfer toward the sublayers, and important time shift (paved stone system); and (iii) low surface temperature during daytime and nighttime, weak heat transfer toward the sublayers, and important time shift (vegetation and substrate system, wood chips system, vegetated urban soil). The results of this study underline that pervious pavements demonstrate thermal benefits under warm and dry summer conditions compared to conventional parking lot solutions. The results also indicate that the hygrothermal properties of urban materials are crucial for urban heat island mitigation.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{doi:10.1080/17518253.2020.1856943,

title = {Biomass of ryegrass from field experiments: toward a cost-effective and efficient biosourced catalyst for the synthesis of Moclobemide},

author = {Marie Hechelski and Christophe Waterlot and Pierrick Dufrénoy and Brice Louvel and Adam Daïch and Alina Ghinet},

url = {https://doi.org/10.1080/17518253.2020.1856943},

doi = {10.1080/17518253.2020.1856943},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Green Chemistry Letters and Reviews},

volume = {14},

number = {1},

pages = {15-22},

publisher = {Taylor & Francis},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{doi:10.1080/00103624.2021.1984505,

title = {Biochar Effects on Soil–Plant Properties after Six Month Adaptation in Soil},

author = {Irina Mikajlo and Brice Louvel and Jaroslav Záhora and Alena Žalmanová and Bertrand Pourrut},

url = {https://doi.org/10.1080/00103624.2021.1984505},

doi = {10.1080/00103624.2021.1984505},

year  = {2021},

date = {2021-01-01},

urldate = {2022-01-01},

journal = {Communications in Soil Science and Plant Analysis},

volume = {53},

number = {1},

pages = {63-76},

publisher = {Taylor & Francis},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{mikajlo2021soil,

title = {Soil Microbial and Physicochemical Changes After the Addition of Biochar, Bacterial Inoculums and Nitrogen Fertilizer},

author = {Irina Mikajlo and Bertrand Pourrut and Brice Louvel and Jaroslav Hynšt and Jaroslav Záhora},

doi = {10.11118/actaun.2021.045},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis},

volume = {69},

pages = {45},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{NSANGANWIMANA2021112125,

title = {Potentials of Miscanthus x giganteus for phytostabilization of trace element-contaminated soils: Ex situ experiment},

author = {Florien Nsanganwimana and Karim Suhail Al Souki and Christophe Waterlot and Francis Douay and Aurélie Pelfrêne and Andrea Ridošková and Brice Louvel and Bertrand Pourrut},

url = {https://www.sciencedirect.com/science/article/pii/S0147651321002360},

doi = {https://doi.org/10.1016/j.ecoenv.2021.112125},

issn = {0147-6513},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {214},

pages = {112125},

abstract = {Phytomanagement is proposed as a cost-effective and environmentally-friendly suggestion for sustainable use of large metal-contaminated areas. In the current work, the energy crop miscanthus (Miscanthus × giganteus) was grown in ex situ conditions on agricultural soils presenting a Cd, Pb and Zn contamination gradient. After 93 days of culture, shoot and root growth parameters were measured. Soils and plants were sampled as well to study the TE accumulation in miscanthus and the effects of this plant on TE mobility in soils. Results demonstrated that miscanthus growth depended more on the soils silt content rather than TE-contamination level. Moreover, soil organic carbon at T93 increased in the soils after miscanthus cultivation by 25.5–45.3%, whereas CaCl2-extractible TEs decreased due to complex rhizosphere processes driving plant mineral uptake, and organic carbon inputs into the rhizosphere. In the contaminated soils, miscanthus accumulated Cd, Pb and Zn mainly in roots (BCF in roots: Cd " Zn > Pb), while strongly reducing the transfer of these elements from soil to all organs and from roots to rhizomes, stems and leaves (average TFs: 0.01–0.06, 0.11–1.15 and 0.09–0.79 corresponding to Cd, Pb and Zn respectively). Therefore, miscanthus could be considered a TE-excluder, hence a potential candidate crop for coupling phytostabilization and biomass production on the studied Metaleurop TE-contaminated soils.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{mikajlo:hal-04288712,

title = {Soil Microbial and Physicochemical Changes After the Addition of Biochar, Bacterial Inoculums and Nitrogen Fertilizer},

author = {Irina Mikajlo and Bertrand Pourrut and Jaroslav Hynv st and Brice Louvel and Jaroslav Záhora},

url = {https://hal.science/hal-04288712},

doi = {10.11118/actaun.2021.045},

year  = {2021},

date = {2021-09-01},

urldate = {2021-09-01},

journal = {Acta Universitatis Agriculturae Et Silviculturae Mendelianae Brunensis},

volume = {69},

number = {4},

pages = {501-510},

publisher = {Mendel University in Brno},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Hechelski2020,

title = {Toward a new way for the valorization of miscanthus biomass produced on metal-contaminated soils Part 1: Mesocosm and field experiments},

author = {Marie Hechelski and Brice Louvel and Pierrick Dufrénoy and Alina Ghinet and Christophe Waterlot},

year  = {2020},

date = {2020-01-01},

journal = {Sustainability},

volume = {12},

pages = {9370},

abstract = {The effects of P-fertilizers (mono- and di-calcium phosphates) on 

	the bioavailability of metals and nutrients in leaves and stems of 

	Miscanthus × giganteus were studied in mesocosm and field experiments 

	in order to propose a new way for the valorization of miscanthus 

	biomass. The concentration of potentially toxic elements was generally 

	higher in stems than in leaves. Although P-fertilizers were added 

	to contaminated soils under sustainable conditions (from 0.022% to 

	0.026% w/w), the average of leaf and stem biomass generally increased 

	in the presence of P-fertilizers due to the changes in the speciation 

	of phosphorus. Leaves of the investigated miscanthus may be of great 

	interest as a catalyst in organic chemistry, since the Ca concentration 

	was up to 9000 mg kg−1 DW. Stems represent a potential biomass that 

	can be used as renewable resource of Lewis acids, currently used 

	in organic syntheses (the sum of Zn, Cu, Mn, Fe, Mg, Si and Al was 

	near 1000 mg kg−1 DW). The percentage of Cd and Pb in leaves and 

	stems of miscanthus did not significantly change with P-fertilizers. 

	Depending on the mesocosm and field experiments, it ranged from 0.004% 

	to 0.016% and from 0.009% and 0.034% for Cd in leaves and stems, 

	respectively, and from 0.004% to 0.015% and from 0.009% and 0.033% 

	for Pb in leaves and stems, respectively.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Mikajlo2020,

title = {Plant-soil nitrogen, carbon and phosphorus content after the addition of biochar, bacterial inoculums and N fertilizer},

author = {Irina Mikajlo and Bertrand Pourrut and Brice Louvel and Jaroslav Hynšt and Jaroslav Záhora},

year  = {2020},

date = {2020-01-01},

journal = {bioRxiv},

volume = {2020-02},

pages = {35pp},

abstract = {The use of biochar in combination with mineral or biological amendments 

	in order to improve its influence on soil-plant properties has received 

	growing attention. The changes of N, C and P content in Lactuca sativa 

	var. capitata aboveground plant biomass and soil after the addition 

	of beech wood biochar combined with the addition of bacterial inoculums 

	(Bacofil and Novarefm) and N fertilizer have been studied using spectrophotometry 

	methods. Pots were filled with the arable soil from the plots in 

	protection zone of water sources (Březová nad Svitavou, South Moravia, 

	Czech Republic). Biochar with inoculums decreased plant growth in 

	the first yield of Novaferm treatment and in both yields of Bactofil 

	treatment. Increased plant biomass growth was observed with Novaferm 

	addition in the second yield. Total N increase has been obtained 

	in the plant aboveground biomass and soil of the treatments amended 

	with inoculums and nitrogen fertilizer. The decrease of P content 

	has been observed in plant aboveground biomass in the biochar amended 

	samples.},

note = {ACLO},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{ALSOUKI2020110654,

title = {Miscanthus x giganteus culture on soils highly contaminated by metals: Modelling leaf decomposition impact on metal mobility and bioavailability in the soil–plant system},

author = {Al Souki Karim Suhail and Clarisse Liné and Brice Louvel and Christophe Waterlot and Francis Douay and Bertrand Pourrut},

url = {https://www.sciencedirect.com/science/article/pii/S0147651320304930},

doi = {https://doi.org/10.1016/j.ecoenv.2020.110654},

issn = {0147-6513},

year  = {2020},

date = {2020-01-01},

urldate = {2020-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {199},

pages = {110654},

abstract = {Miscanthus x giganteus is suggested as a good candidate for phytostabilization of metal-polluted soils. Its late harvest in winter generates large amounts of leaf litter on the soil surface. However, little is known about the mobility and the bioavailability of metals following leaf decomposition and the consequences on the succeeding culture. Ex situ artificial aging for 1, 3, and 6 months was conducted with miscanthus leaf fragments incorporated into three agricultural soils displaying a gradient concentration in Cd (0.6, 3.1 and 7.9 mg kg−1), Pb (32.0, 194.6 and 468.6 mg kg−1), and Zn (48.4, 276.3 and 490.2 mg kg−1) to simulate the leaf litter input over 20 years of miscanthus culture. We investigated the impacts on physicochemical and biological soil parameters, CaCl2-extractable metal, and their subsequent ryegrass shoot concentrations, and hence on ryegrass health. The results showed that the amended soils possessed higher pH along with greater available phosphorous and soil organic carbon values. The respiratory activity and microbial biomass carbon in the amended soils increased mainly after 1 month of aging, and decreased afterwards. Despite the higher Pb- and Zn–CaCl2 extractability in the amended soils, the phytoavailability slightly increased only in the most contaminated soils. Moreover, leaf incorporation did not affect the ryegrass biomass, photosynthetic pigment contents, nor the antioxidative enzyme activities. Conclusively, leaf incorporation induced slight variations in soil physicochemical and biological parameters, as well as metal extractability, but not to an extent that might cause a considerable threat to the subsequent culture. Nevertheless, these results are preliminary data that require confirmation by long-term in-situ experimentations as they reflect the modelization of long-term impact of leaf decomposition on soil-plant system.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Bidar2019,

title = {Influence of amendments on metal environmental and toxicological availability in highly contaminated brownfield and agricultural soils},

author = {Géraldine Bidar and Aurélie Pelfrêne and Brice Louvel and Adeline Janus and Francis Douay},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Science and Pollution Research},

volume = {26},

pages = {33086–33108},

abstract = {The immobilizing effects of wood biochar (BW2%) and iron grit (Z1%) 

	applied alone or in combination (BW2% + Z1%) to agricultural (M750) 

	and brownfield (MAZ) soils highly contaminated by metals were assessed 

	in a greenhouse experiment. The results showed that Z1% and BW2% 

	+ Z1% were the most efficient amendments to reduce Cd, Cu, Pb, and 

	Zn mobility, environmental availability, and phytoavailability in 

	the M750 soil. The oxidation of Z1% allowed part of the Cu and Zn 

	pools present in exchangeable or carbonate-bound forms (labile fraction) 

	to complex in less mobile forms. In this soil, the metal chemical 

	extractions (0.01 M CaCl2 and 0.05 M EDTA) and the DGT (diffusive 

	gradient in thin films) devices to assess metal in soil solution 

	and soil pore water (SPW) also highlighted the immobilizing characteristic 

	of Z1%. In most cases, the addition of BW2% to Z1% (BW2% + Z1%) did 

	not improve this effect, except for the dissolved Pb and Zn concentrations 

	in the M750 soil solution. It was also observed that Cd, Pb, and 

	Zn passed throughout DGT mimicking the biological cell membrane were 

	reduced by all amendments of the M750 soil corroborating metal concentrations 

	measured in rye grass shoots. In the MAZ soil, metals were less available 

	as shown by their low extractability rate, low capacity of metal 

	resupply from the solid phase to pore water, and low phytoavailability. 

	The poor metal availability could be explained by the high levels 

	of carbonate and organic matter contents in this soil. Nevertheless, 

	a decrease of the Cu environmental availability and the Cu concentrations 

	in rye grass shoots grown on the MAZ soil was also observed in the 

	soil amended with Z1% alone or in combination with BW2%. From a health 

	point of view, the most effective amendment to reduce human exposure 

	through ingestion of soil particles for the M750 and MAZ soils was 

	BW2% for Cd and BW2% + Z1% for Pb. However, the presence of rye grass 

	minimized the amendments’ beneficial effects.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Hechelski2019a,

title = {The potential of ryegrass (Lolium perenne L.) to clean up multi-contaminated soils from labile and phytoavailable potentially toxic elements to contribute into a circular economy},

author = {Marie Hechelski and Brice Louvel and Pierrick Dufrénoy and Alina Ghinet and Christophe Waterlot},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Science and Pollution Research},

volume = {26},

number = {17},

pages = {17489–17498},

abstract = {Aided phytoremediation was studied for 48 weeks with the aim of reducing 

	extractable and phytoavailable toxic elements and producing potential 

	marketable biomass. In this sense, biomass of ryegrass was produced 

	under greenhouse on two contaminated garden soils that have been 

	amended with two successive additions of phosphates. After the first 

	addition of phosphates, seeds of ryegrass were sown and shoots were 

	harvested twice. A second seedling was performed after carefully 

	mixing the roots from the first production (used as compost), soils 

	and phosphates. Forty-eight weeks after starting the experiments, 

	the concentrations of Cd, Pb, Zn, Cu, Fe, and Mn extracted using 

	the rhizosphere-based method were generally lower than those measured 

	before the addition of phosphates and cultivation (except for Pb 

	and Fe in the most contaminated soil). The concentrations of metals 

	in the shoots of ryegrass from the second production were lower than 

	those from the first (except for Al). The best results were obtained 

	with phosphates and were the most relevant in the lowest contaminated 

	soil, demonstrating that the available metal concentrations have 

	to be taken into account in the management of contaminated soils. 

	In view of the concentration of metals defined as carcinogens, mutagens, 

	and reprotoxics (e.g., Cd, Pb) and those capable to be transformed 

	into Lewis acids (e.g., Zn, Fe), the utilization of ryegrass in the 

	revegetation of contaminated soils and in risk management may be 

	a new production of marketable biomass. The development of phytomanagement 

	in combination with this type of biomass coincided with the view 

	that contaminated soils can still represent a valuable resource that 

	should be used sustainably.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Leclercq-Dransart2019,

title = {Does adding fly ash to metal-contaminated soils play a role in soil functionality regarding metal availability, litter quality, microbial activity and the community structure of Diptera larvae?},

author = {Julie Leclercq-Dransart and Sylvain Demuynck and Géraldine Bidar and Francis Douay and Fabien Grumiaux and Brice Louvel and Céline Pernin and Alain Leprêtre},

year  = {2019},

date = {2019-01-01},

journal = {Applied Soil Ecology},

volume = {138},

pages = {99-111},

abstract = {This study aimed at evaluating the impact of the use of fly ash-aided 

	phytostabilisation as a method to manage highly metal-contaminated 

	soils on soil functionality taking into account the soil and the 

	litter qualities, the Diptera larvae communities and the microbial 

	activity. To reach this goal, 70 parameters were studied. The main 

	results showed that the two studied fly ashes Soproline® and Sodeline® 

	had a positive influence on soil characteristics especially the availability 

	of metals, nearly 14 years after amendment. Regarding biological 

	parameters, no positive or negative effects were found by adding 

	fly ashes to the soil concerning soil basal respiration and urease 

	activity. The fungal activity was even enhanced. In the same way, 

	the fly ashes had no negative effect on the Diptera larvae community 

	and a greater richness of larvae was even found in amended soils. 

	Thus, this study confirmed that aided-phytostabilisation using these 

	fly ashes should be an advantageous technique to manage metal-polluted 

	soils and to recycle industrial wastes.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Bidar2019b,

title = {Evaluation in situ des effets d’amendements sur le comportement du blé cultivé sur une parcelle agricole contaminée par les activités passées d’une fonderie de plomb},

author = {Géraldine Bidar and Brice Louvel and Adeline Janus and Jacques Blarel and Guillaume Lemoine and Francis Douay},

year  = {2019},

date = {2019-01-01},

booktitle = {4ème Rencontres nationales de la Recherche sur les sites et sols pollués, Le Beffroi de Montrouge, 26-27 novembre 2019, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Bidar2019a,

title = {In situ assessment of soil amendment effects on hemp behavior in soils highly contaminated by past smelter activities},

author = {Géraldine Bidar and Brice Louvel and Adeline Janus and Jacques Blarel and Guillaume Lemoine and Francis Douay},

year  = {2019},

date = {2019-01-01},

booktitle = {Intersol’2019, International Conference-Exhibition on Soils, Sediments and Water, 26-28 mars 2019, Lille},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Hechelski2019b,

title = {Effects of calcium phosphates on the (im)mobilization of metals and nutrients, on the biological activity and on the plant health from multi-contaminated urban soils},

author = {Marie Hechelski and Brice Louvel and Pierrick Dufrénoy and Alina Ghinet and Christophe Waterlot},

year  = {2019},

date = {2019-01-01},

journal = {Water, Air, & Soil Pollution},

volume = {230},

number = {8},

pages = {206-223},

abstract = {Two smelters in the North of France emitted potentially toxic metals 

	for more than a century and today, the resulting contamination represents 

	a risk to human health and affects also the biodiversity. To limit 

	health risks and to improve the soil quality, a study using calcium 

	phosphates (monocalcium phosphate, dicalcium phosphate and a mixture 

	of both salts) and Lolium perenne L was conducted. Through this preliminary 

	investigation, we will try to shed some light about (i) the effects 

	of a sustainable amount of calcium phosphates on the agronomic, biological 

	(microbial and fungi communities) and physiological parameters (chlorophyll 

	a and b, antocyanins, carotenoids) as well as the phytoavailability 

	of potentially toxic metals and nutrients in time, and (ii) the potential 

	use of contaminated biomass from ryegrass as a source of new valorisation 

	ways instead of using it as contaminated compost by gardeners. Although 

	slight variations in pH and significant increases of assimilable 

	phosphorus after adding calcium phosphates were registered, the physiology 

	of plants and the biological parameters were statistically unchanged. 

	The germination of the ryegrass seeds was favoured with calcium phosphates 

	regardless the contamination level of the studied soils. No clear 

	effects of calcium phosphates on the microbial and fungi communities 

	were detected. In contrast, results indicated relationships between 

	the physicochemical parameters of soils, their contamination level 

	and the composition of fungal communities. Indeed, for one of the 

	soils studied, calcium could limit the transport of nutrients, causing 

	an increase in fungi to promote again the transfer of nutrients. 

	Surprisingly, the phytoavailability of Pb increased in the most contaminated 

	soil after adding dicalcium phosphate and the mixture of phosphates 

	whereas a slight decrease was highlighted for Cd and Mn. Although 

	minor changes in the phytoavailability of potentially toxic metals 

	were obtained using calcium phosphates, the ability of ryegrass to 

	accumulate Zn and Ca (up to 600 and 20,000 mg kg−1, respectively) 

	make possible to qualify this plant as a bio ‘ore’ resource.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Hechelski2019ab,

title = {A sustainable approach to manage metal-contaminated soils: a preliminary greenhouse study for the possible production of metal-enriched ryegrass biomass for biosourced catalysts},

author = {Marie Hechelski and Brice Louvel and Pierrick Dufrénoy and Alina Ghinet and Christophe Waterlot},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Monitoring and Assessment},

volume = {191},

number = {10},

pages = {626-640},

abstract = {Two kitchen garden soils (A and B) sampled in contaminated areas were 

	amended using phosphates in sustainable quantities in order to reduce 

	the environmental availability of potentially toxic inorganic elements 

	(PTEs) and to favour the availability of alkali, alkali earth and 

	micronutrients. The environmental availability of PTEs was evaluated 

	using a potential plant for revegetation of contaminated soils (ryegrass) 

	and a mixture of low molecular weight organic acids. Despite the 

	highest contamination level of B, the concentration of metals was 

	highest in the ryegrass shoots grown on A for the two harvests. These 

	results correlated well with those obtained using low molecular weight 

	organic acids for Cd, Zn and Cu, whereas this mixture failed to represent 

	the transfer of nutrients due to the presence of biological and physiological 

	mechanisms. The statistical differences between the biomass of ryegrass 

	obtained at the first and the second harvests were attributed to 

	the decrease of available potassium, implicated in the growth and 

	development of plants. Phosphates increased the ratios Zn/Cd, Zn/Pb 

	and Zn/Cu up to 176 ± 48, 38 ± 6 and 80 ± 12, respectively, and made 

	possible the reduction of the concentration of Cd and Pb in the shoots 

	of ryegrass by 22% and 25%, respectively. The concentration of Zn 

	in the shoots of ryegrass from the first and the second harvests 

	grown on soil A were in the range 1050–2000 mg kg−1, making this 

	plant a potential biomass to (i) produce biosourced catalysts for 

	organic chemistry applications in a circular economy concept and 

	(ii) limit human exposure to commercial Lewis acids. A preliminary 

	application was identified.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Louvel2019,

title = {Développement d’une économie biosourcée franco-belge liée au phytomanagement de sites marginaux},

author = {Brice Louvel and Valérie Bert and Aricia Evlard and Erik Meers},

year  = {2019},

date = {2019-01-01},

booktitle = {4ème Rencontres nationales de la Recherche sur les sites et sols pollués, Le Beffroi de Montrouge, 26-27 novembre 2019, Paris},

abstract = {Le projet Interreg France-Wallonie-Flandres New-C-Land (2018-2021) 

	contribue au développement de l'économie biosourcée et encourage 

	la production durable de biomasse végétale utilisée en énergie et 

	matières sur sites marginaux (surfaces délaissées non cultivées). 

	La COP 21 et le programme Climat et énergie 2030 invitent les pays 

	à réduire leur dépendance aux sources fossiles, à réduire les émissions 

	de gaz à effet de serre et à mettre en oeuvre des activités économiques 

	durables. Dans ce cadre, le projet New-C-Land a reçu le soutien du 

	Fonds européens de développement régional (FEDER) pour identifier 

	des sites abandonnés, sous-utilisés, souvent négligés et parfois 

	pollués. Le projet vise à stimuler la bio-économie sur les 3 territoires 

	en développant sur des surfaces marginales des cultures non alimentaires 

	de biomasses pour être utilisées localement. New-C-Land a pour objectifs 

	(i) d’identifier les terres marginales en Flandre, Wallonie et dans 

	les Hauts de France, (ii) mettre en contact les propriétaires fonciers 

	et les utilisateurs de la biomasse afin d’encourager le développement 

	de chaînes de valeur industrielles, (iii) apporter un outil cartographique 

	et d’aide à la décision et (iv) tester les filières de valorisation 

	identifiées grâce à l’outil d’aide à la décision in situ sur pilotes 

	et à l’échelle industrielle.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Louvel2019a,

title = {Évaluation de la phytodisponibilité des éléments métalliques présents dans un digestat solide épandu sur une terre contaminée ou non},

author = {Brice Louvel and Mathieu Bossuwe and Francis Douay},

year  = {2019},

date = {2019-01-01},

booktitle = {4ème Rencontres nationales de la Recherche sur les sites et sols pollués, Le Beffroi de Montrouge, 26-27 novembre 2019, Paris},

abstract = {L’épandage de digestats de méthanisation, issus d’intrants agricoles 

	provenant d’une zone contaminée en métaux, interroge sur le devenir 

	des polluants et le comportement des cultures. Pour apporter des 

	éléments de réponse, des digestats de méthanisation ont été élaborés 

	à partir de productions issues ou non de la zone affectée massivement 

	par les émissions passées de Metaleurop Nord (Noyelles-Godault). 

	Une culture de ray-grass a été réalisée en serre sur deux terres 

	prélevées dans l’horizon labouré de deux parcelles situées ou non 

	sous l’influence de l’ancienne fonderie de plomb et de zinc. Ce protocole 

	expérimental a été complété avec une modalité comportant une fertilisation 

	minérale NPK et un témoin (eau). Sur la terre non contaminée, l’ajout 

	des digestats solides contaminés ou non n’a pas eu d’effet sur le 

	rendement des feuilles de ray-grass alors que sur la terre contaminée 

	a été constaté un accroissement notable de la biomasse. La contamination 

	de la terre contaminée a été soulignée par la modalité témoin (eau) 

	; les concentrations en Cd, Pb et Zn dans les parties aériennes du 

	ray-grass cultivé sur la terre contaminée sont respectivement près 

	de 5, 2 et 5 fois plus élevées que celles se rapportant au ray-grass 

	récolté sur la terre non contaminée. Les concentrations en Cd et 

	Zn du ray-grass cultivé sur la terre non contaminée amendée n’ont 

	pas montré de différence avec celles du témoin. L’apport de digestat 

	issu des productions de la zone Metaleurop a augmenté les concentrations 

	en Cd et Zn dans les feuilles de ray-grass cultivé sur la terre contaminée 

	par rapport au témoin. Toutefois, cet accroissement n’a pas été significativement 

	différent de celui constaté avec l’apport de digestat issu de végétaux 

	non contaminés ou de fertilisants minéraux. Ceci laisse donc penser 

	que l’accroissement des concentrations en métaux dans le ray-grass 

	est davantage lié à la contamination des sols qu’aux contaminants 

	apportés avec les digestats.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Louvel2019b,

title = {Expérimentation de phytomanagement sur les sols de l’ancien site Rhodia de Saint-André-Lez-Lille},

author = {Brice Louvel and Tanguy Latron and Damien Delsarte and Francis Douay},

year  = {2019},

date = {2019-01-01},

booktitle = {4ème Rencontres nationales de la Recherche sur les sites et sols pollués, Le Beffroi de Montrouge, 26-27 novembre 2019, Paris},

abstract = {La reconquête des friches industrielles en coeur des métropoles est 

	un enjeu majeur dans le cadre du renouvellement urbain. La période 

	de transition vers un nouvel usage des sites peut s’échelonner sur 

	plusieurs années. Pendant ce laps de temps, il est important de répondre 

	aux attentes environnementales (confinement des contaminants sur 

	le site…) et sociétale (amélioration du cadre de vie des riverains…). 

	Les couvertures végétales peuvent apporter une réponse à ces contraintes. 

	La diversité des espèces végétales couramment utilisées est cependant 

	restreinte. Une expérimentation de phytomanagement on-site a été 

	mise en place de 2015 à 2018 sur un ancien site industriel lié à 

	la chimie minérale et ceci, en vue d’évaluer l’aptitude de différents 

	couverts végétaux à se développer sur des matériaux multi-contaminés 

	(fortes anomalies en As, Hg et Pb). Une plateforme expérimentale 

	comportant 14 placettes a été créée pour être dédiée à l’étude de 

	12 espèces végétales sélectionnées au regard de leur comportement 

	vis-à-vis des polluants métalliques et de leur aptitude à les accumuler 

	dans leurs organes aériens (Medicago lupulina, Brassica juncea, Agrostis 

	capillaris, Salix alba, Lolium perenne, Anthyllis vulneraria, Festuca 

	rubra, Festuca ovina, Achillea millefolium, Deschampsia cespitosa, 

	Valeriana officinalis, Miscanthus x giganteus). Lolium perenne a 

	été semé seul ou en association avec Salix alba. Après trois années 

	de suivi, seules quatre espèces initialement installées étaient encore 

	présentes se sont adaptées aux conditions expérimentales. Les teneurs 

	en contaminants inorganiques (As, Cd, Cr, Zn) dans les parties aériennes 

	ont été supérieures dans le Miscanthus x giganteus par rapport à 

	celles mesurées dans Agrostis capillaris, Festuca ovina et Festuca 

	rubra.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Louvel2019c,

title = {Evaluation in situ de modes de gestion appliqués sur une friche multicontaminée de la carbochimie},

author = {Brice Louvel and Guillaume Lemoine and Sébastien Détriché and Géraldine Bidar and Adeline Janus and Francis Douay},

year  = {2019},

date = {2019-01-01},

booktitle = {4ème Rencontres nationales de la Recherche sur les sites et sols pollués, Le Beffroi de Montrouge, 26-27 novembre 2019, Paris},

abstract = {La reconquête des friches industrielles présente des enjeux environnementaux 

	et sanitaires d’envergure. Dans cet objectif, les phytotechnologies 

	sont présentées dans la littérature comme un mode de gestion innovant, 

	transitoire ou pérenne, et limitant la dispersion des polluants en 

	protégeant les sols de l’érosion. Elles peuvent réduire la disponibilité 

	des polluants. Néanmoins, ces techniques manquent encore de recul 

	opérationnel [1]. Le projet MisChar, soutenu par l’ADEME, vise à 

	apporter un regard scientifique, technique et économique sur différents 

	modes de gestion appliqués sur des sols multicontaminés par des activités 

	industrielles passées. Une expérimentation a été mise en place avec 

	pour objectif d’étudier les effets de modes de gestion basés sur 

	l’utilisation d’un amendement des sols, l’installation de communautés 

	végétales et des pratiques usuelles d’entretien des espaces verts. 

	Elle concerne une parcelle située dans l’ancien Bassin minier du 

	Nord – Pas de Calais sur laquelle ont été déposés des matériaux multicontaminés 

	issus de la carbochimie (usines de Mazingarbe). Au printemps 2018, 

	20 placettes d’une surface de 20 m² ont été définies. Le sol de 10 

	de ces placettes a été amendé au moyen d’un mélange de biochar de 

	miscanthus et de compost de déchets verts. Les 10 autres placettes 

	ont été utilisées comme témoin. A chacune de ces placettes correspond 

	un type d’ensemencement particulier et un mode de gestion distinct. 

	Ainsi, diverses essences végétales ont été semées. Il s’agit (1) 

	d’un mélange d’espèces classiquement mis en oeuvre sur les espaces 

	verts (ray-grass anglais, fétuque rouge gazonnante, fétuque rouge 

	traçante et pâturin des prés), (2) d’un mélange d’espèces sélectionnées 

	pour leurs comportements présumés vis-à-vis des polluants (stabilisation 

	des polluants métalliques, rhizo/phytodégradation de certains polluants 

	organiques (ray-grass anglais, agrostide stolonifère, fétuque rouge, 

	trèfle blanc), et des mêmes espèces en implantation monospécifique 

	avec (3) un ray-grass, (4) une agrostis stolonifère, (5) un trèfle 

	blanc, et (6) une fétuque rouge. Une septième modalité correspond 

	à l’installation d’une végétation spontanée. Aux espèces implantées 

	en mélange et à la végétation spontanée, correspondent deux modes 

	de gestion (tonte ou pas du couvert végétal). Durant les trois années 

	d’expérimentation seront étudiés le comportement des polluants des 

	sols, les effets des modes de gestion sur les communautés végétales 

	et d’une façon globale, sur la fonctionnalité des sols.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Waterlot2019b,

title = {Benefits of Ryegrass on Multicontaminated Soils Part 2: Green process to provide Idrocilamide},

author = {Christophe Waterlot and Pierrick Dufrénoy and Marie Hechelski and Brice Louvel and Adam Daïch and Alina Ghinet},

year  = {2019},

date = {2019-01-01},

journal = {Sustainability},

volume = {11},

number = {23},

pages = {6685-6694},

abstract = {A restoration of highly contaminated garden soil is proposed as a 

	greener alternative to the production of vegetables. Depending on 

	potentially toxic elements and their concentration, ryegrass shoots 

	accumulate these elements in sufficient quantity to be used as a 

	catalyst in organic synthesis. The analysis of Cd, Pb, Zn, Cu, Fe, 

	Mn, and Al in ashes issued from the calcination of ryegrass shoots 

	revealed that the concentration of Zn was highest (>7000 mg kg–1). 

	The ratios between potential Lewis acids (Zn, Cu, Fe, Mn, and Al) 

	to carcinogenic metals (Cd or Pb) were 191 ± 7 for LA/Cd and 235 

	± 13 for LA/Pb, making the shoots of ryegrass suitable for the production 

	of Zn-rich polymetallic biosourced catalysts. This material was used 

	in the synthesis of idrocilamide under free-solvent condition, providing 

	the drug in a good yield (69.9%). Data show that a limitation of 

	waste, a maximization of the material incorporation in the process, 

	a minimization of the steps, and an optimization of the stoichiometric 

	factor are the main innovative factors in the current process in 

	comparison with those previously reported.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@techreport{Chalmandrier2019,

title = {Retour d’expérience sur la mise en place d’une expérimentation sur une friche péri-urbaine contaminée : dépasser la notion «d’acceptabilité sociale» pour faire du sol un bien commun},

author = {Chalmandrier Maud and Douay Francis and Brice Louvel and Petit-Berghem Yves and Rémy Elisabeth},

year  = {2019},

date = {2019-01-01},

urldate = {2019-01-01},

note = {VAL},

keywords = {ER4},

pubstate = {published},

tppubtype = {techreport}

}

@conference{Hechelski2018,

title = {An original approach in green chemistry: From assisted-phytoremediation of contaminated soil to upcycling of plant biomass for biosourced catalyst production},

author = {Marie Hechelski and Pierrick Dufrénoy and Brice Louvel and Alina Ghinet and Christophe Waterlot},

year  = {2018},

date = {2018-01-01},

booktitle = {International Conference Contaminated Sites 2018, 8-10 October 2018, Banská Bystrica (Slovak Republic)},

abstract = {A kitchen garden soil was sampled in a contaminated urban area located 

	in the north of France. Samples were air-dried and crushed to pass 

	through a 10-mm stainless steel sieve. Due to the high heterogeneity 

	of garden soils in the studied area, much attention have been paid 

	on the homogeneity of soil samples. After this step, the mass of 

	sampled soils (48 kg) was divided to obtain four subsamples Each 

	of them was divided in six replicates, unamended or amended using 

	dicalcium phosphate (DCP) or monocalcium phosphate (MCP) or a mixture of these two compounds (MxP=75%DCP+25%MCP) The phosphorous amendments 

	were added into the soil in small quantity (0.02 %) with the aim 

	at reducing the environmental availability of carcinogenic metals 

	and to increase the Zn availability (Figure 1) After the stabilisation 

	period (2 months) in a greenhouse, 1 5 g of ryegrass seeds (Lolium 

	perenne L.) were sown in the 24 containers. Eight weeks after sowing, 

	ryegrass shoots were harvested, oven-dried at 40 °C and calcined 

	at 500 °C in a muffle furnace (Nabertherm P330, Lilienthal, Germany).},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Hechelski2018a,

title = {From Conventional Lewis Acids to Heterogeneous Montmorillonite K10: Eco-Friendly Plant-Based Catalysts Used as Green Lewis Acids},

author = {Marie Hechelski and Alina Ghinet and Brice Louvel and Pierrick Dufrénoy and Benoît Rigo and Adam Daïch and Christophe Waterlot},

year  = {2018},

date = {2018-01-01},

journal = {ChemSusChem},

volume = {11},

number = {8},

pages = {1249-1277},

abstract = {The concept of green chemistry began in the USA in the 1990s. Since 

	the publication of the 12 principles of this concept, many reactions 

	in organic chemistry have been developed, and chemical products have 

	been synthesized under environmentally friendly conditions. Lewis 

	acid mediated synthetic transformations are by far the most numerous 

	and best studied. However, the use of certain Lewis acids may cause 

	risks to environmental and human health. This Review discusses the 

	evolution of Lewis acid catalyzed reactions from a homogeneous liquid 

	phase to the solid phase to yield the expected organic molecules 

	under green, safe conditions. In particular, recent developments 

	and applications of biosourced catalysts from plants are highlighted.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Hechelski2018b,

title = {Phytomanagement of contaminated soils by potentially toxic metals in urban areas: A new approach combining sustainable amount of phosphates and ryegrass},

author = {Marie Hechelski and Brice Louvel and Pierrick Dufrénoy and Alina Ghinet and Christophe Waterlot},

year  = {2018},

date = {2018-01-01},

booktitle = {26e Réunion des Sciences de la Terre, 22-26 octobre 2018, Lille},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Janus2018,

title = {Influence of two amendments on phyto- and sanitary availability of metals in highly contaminated soils: A greenhouse study},

author = {Adeline Janus and Aurélie Pelfrêne and Géraldine Bidar and Brice Louvel and Francis Douay},

year  = {2018},

date = {2018-01-01},

booktitle = {International Conference Contaminated Sites 2018, 8-10 October 2018, Banská Bystrica (Slovak Republic)},

pages = {101},

abstract = {Soil is an essential and non-renewable resource which can perform 

	a high number of economic, social and environmental functions as 

	biomass production, source of raw materials or protection of humans 

	and environment (Blum, 2005). However, the soil functionality becomes 

	increasingly compromised due to contaminations caused by human activities. 

	In 2006 and in 39 countries, the European Environmental Agency inventoried 

	approximately 3 million of sites where pollutant activities occurred 

	with more than 1 .8 million potentially contaminated sites (CGDD 

	2013). In 2012, the most frequently identified contaminants were 

	metals (35 %), hydrocarbons (24 %) and polycyclic aromatic hydrocarbons 

	(11 %). Until recently, the most common remediation technique was 

	the excavation of contaminated soil and its disposal as landfill. 

	However, this kind of method is considered inappropriate because 

	it generates considerable disturbances, is expensive and economically 

	unfeasible on a large scale . Thus, other remediation techniques 

	(ex and in situ) have been developed to overcome these disadvantages 

	. Among them, a technique consists in adding inorganic or organic 

	amendments to the contaminated soils in order to decrease the mobility 

	and bioavailability of pollutants in soils (Kumpiene et al., 2008; 

	Vangronsveld et al., 2009; Bolan et al., 2014; Nejad et al., 2017) 

	. The most often used amendments are phosphate compounds, liming 

	materials, metal oxides and biochars, used alone or in combination 

	(Waterlot et al., 2017; Lahori et al., 2017; Oustrière et al., 2017). 

	The goal of the present work consists in evaluating the ability of 

	two amendments (woody biochar and iron grit, used alone or in combination) 

	to immobilize Cd, Cu, Pb, and Zn in contaminated soils under greenhouse 

	conditions},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Pottiez2018,

title = {Reconception durable de deux systèmes grandes cultures et légumiers pour réduire de moitié l'utilisation des produits phytosanitaires},

author = {Bruno Pottiez and S Allexandre and M Grouselle-Labouysse and A Mollet and K Petit and J Bruyere and Sandrine Oste and F Simeon and F Delassus and V Delannoy and Brice Louvel and Francis Douay and Dominique Werbrouck and Ali Siah and Patrice Halama},

year  = {2018},

date = {2018-01-01},

journal = {Innovations Agronomiques},

volume = {70},

pages = {305-316},

abstract = {Cette étude, conduite dans le cadre du dispositif DEPHY EXPE Ecophyto, 

	sur une durée de six ans, vise à utiliser des leviers agronomiques 

	et techniques en vue de réduire de 50 % l’utilisation des produits 

	phytopharmaceutiques conventionnels dans deux systèmes de culture 

	(grandes cultures et cultures légumières). Après cinq années d’expérimentation, 

	la majorité des règles de décision ont été appliquées et la faisabilité 

	de certains leviers mis en place a également été analysée. Ont également 

	été identifiés des problèmes non résolus avec la technicité disponible, 

	comme par exemple la maîtrise des bio-agresseurs du pois de conserve, 

	le désherbage de l’oignon et la modulation des seuils de nuisibilité 

	selon les niveaux de populations des auxiliaires. Les résultats mettent 

	en évidence, en moyenne, des niveaux de performance moindres dans 

	la conduite IFT50 (réduction de 50 % des IFT à l’échelle du système), 

	avec des variations selon la culture et l’année considérée. Ces performances 

	sont toutefois encourageantes et peuvent être améliorées afin d’optimiser 

	l’apport de certains leviers},

note = {ACLN},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Leclercq-Dransart2018,

title = {Litter breakdown as a tool for assessment of the efficiency of afforestation and ash-aided phytostabilisation on metal contaminated soils functioning in Northern France},

author = {Julie Leclercq-Dransart and Lucia Santorufo and Céline Pernin and Brice Louvel and Sylvain Demuynck and Fabien Grumiaux and Francis Douay and Alain Leprêtre},

year  = {2018},

date = {2018-01-01},

journal = {Environmental Science and Pollution Research},

abstract = {The main objective of the study was to assess the efficiency of phytoremediation methods implemented for 14 years on highly metal contaminated soils. The different experimental strategies were plots planted with a tree mix or with a single tree species coupled or not with the use of fly-ashes as an amendment to limit metals mobility in soil. The breakdown of poplar litter on the four plots was monitored during 10 months. In parallel, colonization of litter bags by functional groups of mesofauna (Collembola and Acari) was followed. Two mesh sized litter-bags were used to allow distinguishing microbial and mesofaunal actions	on the litter breakdown. We observed the breakdown of litter in four 

studied plots. Litter breakdown occurred faster in 3 mm litter bags than 250 µm ones during summer demonstrating the importance of mesofauna. Mixed plantation allowed faster litter breakdown than mono-specific plantation. A higher abundance of mesofauna and/or better abiotic conditions (moisture, shading…) could explain this result. Regarding litter breakdown and mesofauna, no significant difference was observed between the amended plots and those subjected to soil phytomanagement.	However, communities of the studied area are disturbed since a low abundance of detritivores was observed. This could explain also the slower litter breakdown than expected in our study. To conclude, 

among the phytomanagement methods tested, mixed plantations could provide a benefit for the restoration of degraded soils. By contrast, the use of fly-ashes does not seem to have any effect on the functionality	of ecosystem neither on the litter breakdown process nor on the abundance of mesofauna.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Al-Souki2017a,

title = {Interest of Miscanthus x Giganteus in phytomanaging heavy metal contaminated soils},

author = {Karim Al-Souki and Brice Louvel and Francis Douay and Bertrand Pourrut},

year  = {2017},

date = {2017-01-01},

booktitle = {2nd World Congress on Civil, Structural, and Environmental Engineering (CSEE’17), 2-4 April 2017, Barcelona (Espagne)},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Al-Souki2017c,

title = {Assessment of Miscanthus x giganteus capacity to restore the functionality of metal-contaminated soils: Ex situ experiment},

author = {Karim-Suhail Al-Souki and Brice Louvel and Francis Douay and Bertrand Pourrut},

year  = {2017},

date = {2017-01-01},

journal = {Applied Soil Ecology},

volume = {115},

pages = {44-52},

abstract = {Phytostabilization can be an appropriate choice for managing soils contaminated by diverse metals. Miscanthus x giganteus was selected to phytostabilize polluted soils surrounding the former lead smelter Metaleurop Nord, in Northern France. The aim of the current study was to determine the impact of miscanthus plants on restoring the corresponding soil functionality. Ex situ experiments revealed that soil biological activities (basal respiration, fluorescein diacetate hydrolytic activity, acid phosphatases, laccases and ureases) as well as microbial biomass carbon increased in contaminated soils cultivated with miscanthus, compared to unplanted soils. These results verify the capacity of miscanthus to restore polluted soil functionalities, indicating the positive effect of phytostabilization on soil biological activities. In conclusion, revegetating polluted soils with miscanthus plants can restore their capacity to perform biochemically and biologically.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Hechelski2017,

title = {Toward a new approach in drug design: from polluted soil an non hyper-accumulating plants to anti-inflammatory agent},

author = {Marie Hechelski and Pierrick Dufrénoy and Brice Louvel and Alina Ghinet and Christophe Waterlot},

year  = {2017},

date = {2017-01-01},

booktitle = {4th French-Romanian Colloquium on Medicinal Chemistry, 5-7 October 2017, Iasi (Roumanie)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pottiez2017,

title = {Utilisation de leviers agronomiques et techniques dans deux systemes de cultures en vue de reduire de 50% l'utilisation des produits phytopharmaceutiques dans le Nord de la France},

author = {B Pottiez and S Allexandre and M Labouysse and K Petit and J Bruyere and V Delannoy and Brice Louvel and Francis Douay and Sandrine Oste and D Werbrouck and Ali Siah and Patrice Halama},

year  = {2017},

date = {2017-01-01},

booktitle = {6e COMAPPI, Conférence sur les Moyens Alternatifs de Protection pour une Production Intégrée, 21-23 mars 2017, Lille},

pages = {660-669},

abstract = {This study, conducted in the framework of the Ecophyto network over 

	a six-year period, aim at using ergonomic and technical levers in 

	order to reduce by 50% the use of conventional phytosanitary products 

	in two cropping systems (arable and vegetable crops). After three 

	years of experimentation, the majority of the expected technics were 

	used and the application of some levers was analyzed. Some problems 

	without solutions were identified, such as the control of bio-aggressors 

	in canned peas, weeding in onion and modulation of thresholds regarding 

	populations of auxiliaries. Results showed a lower performance of 

	IFT50 conditions (reduced by 50% in the use of phytosanitary products 

	at the cropping system scale) that varies depending on the considered 

	crop and year. However, the performances obtained are encouraging 

	and can be further improved by the end of the project},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Leclercq-Dransart2017c,

title = {Efficacité de méthodes de phytomanagement sur la fonctionnalité de sols fortement contaminés par les métaux},

author = {Julie Leclercq-Dransart and Lucia Santorufo and Céline Pernin and Brice Louvel and Sylvain Demuynck and Fabien Grumiaux and Francis Douay and Alain Leprêtre},

year  = {2017},

date = {2017-01-01},

booktitle = {Société Française d'Ecotoxicologie Fondamentale et Appliquée, 29-30 juin 2017, Lille},

abstract = {Le site atelier metaleurop situé dans l'ancien bassin minier du nord - pas de calais, présente des paysages fortement dégradés par les activités humaines (urbanisation, activités industrielles, agriculture intensive). la forte contamination des sols en métaux aux alentours de l’ancienne fonderie metaleurop nord est bien connue et plusieurs études ont été menées sur le secteur (e.g: comportement des métaux dans le sol et phytomanagement [1,2], risques sanitaires [3] et communautés biologiques [4,5]). une expérimentation a été mise en place sur le terrain pendant 10 mois avec pour objectif d'évaluer l'efficacité de méthodes de remédiation mises en place depuis 17 ans sur des sols contaminés en métaux et fortement dégradés. il s'agit de plantations mixtes ou mono-spécifiques couplées ou non à l'utilisation de cendres volantes comme amendement pour limiter la mobilité des métaux dans le sol. l'approche mise en oeuvre est basée sur le suivi de la dégradation d'une litière de peuplier selon les différents modes de gestion et sa colonisation par les organismes de la mésofaune, connus pour intervenir lors de la dégradation de la matière organique et fournir de nombreux services écosystémiques [6]. la technique des sacs de litière a été utilisée. les principaux résultats montrent que la dégradation de la litière est significativement plus rapide sur la plantation mixte que la monospécifique en été ce qui pourrait s'expliquer par une plus forte abondance de la mésofaune et/ou des conditions abiotiques (humidité, ombrage…) plus favorables à l'activité de celle-ci. en revanche, aucune différence significative n'a été observée entre les parcelles amendées (dégradation de la litière apportée et communautés mésofauniques) et celle soumise au seul phytomanagement. on note que les communautés sont perturbées et que l'abondance des organismes détritivores est faible ce qui pourrait expliquer une dégradation des litières plus lente qu'attendue. ainsi, parmi les méthodes de phytomanagement testées sur le site expérimental, les plantations mixtes pourraient apporter un bénéfice pour la restauration des sols dégradés. l'emploi de cendres volantes n'a pas d'incidence notable sur la fonctionnalité des sols.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Nsanganwimana2016,

title = {Metal, nutrient and biomass accumulation during the growing cycle of Miscanthus established on metal-contaminated soils},

author = {Florien Nsanganwimana and Christophe Waterlot and Brice Louvel and Bertrand Pourrut and Francis Douay},

year  = {2016},

date = {2016-01-01},

journal = {Journal of Plant Nutrition and Soil Science},

volume = {179},

number = {2},

pages = {257-269},

abstract = {The energy crop miscanthus presents high potentials for phytomanagement. its shoot yield and nutrient accumulation has been extensively characterized in uncontaminated agricultural soils, while very little is known for metal-contaminated conditions. this study aimed at assessing potential differences in dry matter and metal and nutrient accumulation of the standing aerial biomass in miscanthus (m. x giganteus) growing in situ on agricultural plots presenting different soil cd, pb, and zn concentrations. plant samplings were conducted monthly along the growing period from may to december. cadmium, pb, zn, and the concentrations of the nutrients n, p, k, ca, mg, and na were determined in leaves and stems separately. during the growing phase, the maximum dry matter was reached in early in autumn. whatever the organ, cd and zn concentrations were higher on contaminated than on uncontaminated plots. during summer and autumn, zn and pb concentrations were higher in leaves than in stems whereas cd concentrations did not significantly differ between the organs. concentrations of n, p, k, and mg decreased across the study period whereas those of ca and na increased. overall, metal and nutrient concentrations depended on plant organ and its development stage. the dry matter and nutrient accumulation patterns were not different between contaminated and uncontaminated plots. the significance of these findings is discussed in light of best phytomanagement practices and potential uses of miscanthus biomass.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Goulas2015,

title = {Analytical method for determining polycyclic aromatic hydrocarbon pollutants using ultra-fast liquid chromatography with fluorescence detection and the recent column packed with the new 5 μm Kinetex-C18 core-shell particles},

author = {Anaïs Goulas and Brice Louvel and Christophe Waterlot},

year  = {2015},

date = {2015-01-01},

journal = {Canadian Journal of Chemistry},

volume = {93},

number = {5},

pages = {564-571},

abstract = {Nous avons optimisé une méthode de chromatographie liquide à ultra haute performance à détection par fluorescence pour réaliser le dosage de 15 hydrocarbures aromatiques polycycliques (hap) en employant la nouvelle colonne kinetex-c18 (250 mm × 4,6 mm). nous avons utilisé cette colonne récemment commercialisée, contenant un nouveau type de particules poreuses de type core-shell dont la taille moyenne est de 5 μm et fonctionnant à très basse pression, afin de séparer divers composés par chromatographie liquide. après optimisation de la méthode analytique, la séparation des 15 hap dans des échantillons d’eau du robinet dopés a été réalisée, sans coélution de produits, en 21,5 min à 16 °c, dans une phase mobile qui consistait en un mélange aqueux d’acétonitrile, en conditions de gradient de concentrations, à très faible débit (0,7 à 1,0 ml min−1) et à faible pression (870 à 1 590 psi = 60 à 110 bar); toutes ces conditions présentant un intérêt sur le plan économique. pour chacun des composés, nous avons effectué la synchronisation entre les variations de longueur d’onde dans le temps et le temps d’élution dans le but de prévenir une dérive de la ligne de base. nous avons mené la validation de l’ensemble de la procédure expérimentale en tenant compte des paramètres suivants : courbe de calibration, linéarité, limites de détection et de quantification, exactitude, sensibilité, précision et répétabilité du temps de rétention de chaque hap. la méthode analytique proposée présentait une bonne linéarité dans la gamme de concentrations entre 0,025 et 10 μg l−1, où le coefficient de corrélation était supérieur à 0,9980. la répétabilité (écart-type relatif en pour cent},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Nsanganwimana2015,

title = {Metal accumulation and shoot yield of Miscanthus × giganteus growing in contaminated agricultural soils: Insights into agronomic practices},

author = {Florien Nsanganwimana and Bertrand Pourrut and Christophe Waterlot and Brice Louvel and Géraldine Bidar and Sonia Labidi and Joël Fontaine and Jérôme Muchembled and Anissa Lounès-Hadj-Sahraoui and Hervé Fourrier and Francis Douay},

year  = {2015},

date = {2015-01-01},

journal = {Agriculture, Ecosystems & Environment},

volume = {213},

pages = {61-71},

abstract = {The choice of agronomic practices for phytomanagement of metal-contaminated soils is of crucial importance to optimize plant biomass yields and to mitigate both environmental and health risks due to metal exposure. the present study aimed to assess the effects of agronomic practices on shoot yield and on metal (cd, pb, and zn) accumulation in the organs of the energy crop miscanthus (miscanthus × giganteus) during the first three years since the plantation on metal-contaminated agricultural soils. three miscanthus cultivars, hereafter named mis-a, mis-b and mis-i, were planted at low and high density. an inoculum of arbuscular mycorrhizal fungi-amf (glomus lpa val 1) was added during plantation, and nitrogen fertilization was applied during the third growing season. metal accumulation in miscanthus organs was determined during the second growing season, whereas shoot yields and their metal concentrations were determined during both the second and the third growing seasons. based on metal concentrations and bioconcentration factors, the three cultivars mainly accumulated metals in their roots. the shoot yields increased from 3.7 to 10.3 t dw ha−1 in the second growing season to 15.8–23.3 t dw ha−1 in third growing season. there were no or very few significant differences in metal concentrations and shoot yields within treatments comprising the same cultivar. the addition of amf inoculum increased metal (mainly cd and zn) accumulation in miscanthus organs and in the shoot yields and this was more observed in both mis-b and mis-i which presented a higher root mycorrhization level than in mis-a. shoot yields in treatments comprising different cultivars depended not on fertilization but on the interactions between cultivar and planting density, and between cultivar, planting density and amf inoculum. whatever the treatment and the sampling period, pb concentrations did not significantly differ in shoot yields. the interaction between cultivar and planting density resulted in higher cd concentrations in the yields of mis-b planted at low density during the third growing season. zn concentrations increased with fertilization in all treatments, and with the addition of the amf inoculum in mis-b and in mis-i. overall, the results demonstrated that the three cultivars could be potential candidates for coupling phytostabilization and biomass production on metal-contaminated soils.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Pottiez2015,

title = {Intégration de méthodes de lutte alternative dans des systèmes de culture pour réduire de 50% l’utilisation des produits conventionnels de protection des plantes},

author = {B Pottiez and S Allexandre and E Craye and F Couturier and G Billaut and K Petit and J Bruyere and V Delannoy and Brice Louvel and Francis Douay and S Oste and D Werbrouck and Ali Siah and Patrice Halama},

year  = {2015},

date = {2015-01-01},

booktitle = {5th International Conference on Alternative Methods of Crop Protection, 11-13 mars 2015, Lille},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Sahmer2015,

title = {Utilisation d'une régression non linéaire pour des applications microbiologiques},

author = {Karin Sahmer and Caroline Deweer and Jérôme Muchembled and Lucia Santorufo and Brice Louvel and Francis Douay and Patrice Halama},

year  = {2015},

date = {2015-01-01},

booktitle = {Séminaire Modal (MOdels for Data Analysis and Learning), INRIA, 13 janvier 2015, Lille},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Bidar2014,

title = {Effets à moyen terme d’une phytostabilisation aidée sur la distribution verticale des paramètres physico-chimiques et des éléments traces métalliques dans les sols},

author = {Géraldine Bidar and Brice Louvel and Nicolas Proix and Christophe Waterlot and Hervé Fourrier and Antoine Richard and Francis Douay},

year  = {2014},

date = {2014-01-01},

booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Burrow2014,

title = {Influence des modalités de gestion d'un technosol sur le maintien de communautés de collemboles - Expérimentations en microcosmes},

author = {Coralie Burrow and Brice Louvel and Céline Pernin and Alain Leprêtre},

year  = {2014},

date = {2014-01-01},

booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris},

abstract = {Objectifs tester 3 modes de gestion - brf, paillis et semis - déjà expérimentés aux niveaux supérieurs (placettes in situ, mésocosmes) en analysant plus finement les préférences des collemboles en termes d’habitat . ces collemboles ont un rôle actif dans les processus de minéralisation de la matière organique et sont considérés comme étant de bons indicateurs de la qualité et du fonctionnement d'un sol},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Dumat2014,

title = {Evaluation de la qualité des plantes potagères: guide d’échantillonnage et bases de données},

author = {Camille Dumat and Elodie-Denise Chenot and Bertrand Pourrut and B Schwartz and P Leglize and S Ouvrard and Brice Louvel and Francis Douay and C Hulot and Franck Marot},

year  = {2014},

date = {2014-01-01},

booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Louvel2014,

title = {Influence de la phytostabilisation aidée sur les activités microbiennes dans des sols contaminés par des éléments traces métalliques},

author = {Brice Louvel and Hervé Fourrier and Francis Douay},

year  = {2014},

date = {2014-01-01},

booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Leclercq2014a,

title = {Can mulching boost ecological connectivity between different management options in a disturbed environment?},

author = {Julie Leclercq-Dransart and Lucia Santorufo and Céline Pernin and Sylvain Demuynck and Brice Louvel and Fabien Grumiaux and Sébastien Détriché and Francis Douay and Alain Leprêtre},

year  = {2014},

date = {2014-01-01},

booktitle = {First Global Soil Biodiversity Conference - Assessing soil biodiversity and its role for ecosystem services, 2-5 décembre 2014, Dijon},

abstract = {Today, rare are unfragmented habitats. the landscapes are transformed by human activities and species are threatened: isolation, downsizing, reduced trade between populations... the former coal mining area of northern france is not an exception: urbanization, industry, intensive agriculture and metal pollution are the main causes of land fragmentation and disturbance. it is important to look for ways to reconnect these isolated and degraded areas. the present study aimed at evaluating the attractivity of different kind of mulches and their efficiency to promote recolonization of isolated and degrades areas. since march 2013, 18 experimental rows (6m long x 0.5 m width) of mulch were installed between two wood plots (pluri and monospecific plantations) on a surface area which stretch on 4400 m². five kind of mulch (ramial chipped wood (rcw), dead leaves, chopped miscanthus, chopped wheat straw and canvas cover) and a control (bare soil) were tested (3 replicates each). the control consisted in 3 replicated of bare soil. each mulch was characterized by the loss of biomass with litter bag technique, metal concentrations, temporal monitoring of enzymatic activities and organic matter, etc. the attendance of these mulches by pedofauna was followed using both interception and emergence traps. the formers are wet pitfall traps revealing the activity intensity of aboveground fauna. the latter track imagos of soil insects. the trapping of pedofauna was carried out from april to july 2014 using 90 pitfall traps and 78 soil emergence traps (no trap on canvas cover). a general inventory of pedofauna trapped was made, focusing on two groups models: groundbeetles (carabidae) and woodlice (isopoda), which belong to two different trophic groups, predators and saprophageous. first results show that mulches welcome more organisms than bare soil. leaves and rcw are the modalities with the highest abundances. more detailed results will be presented and discussed.},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Leclercq2014,

title = {Contribution à la restauration d’une biodiversité dans un contexte péri-industriel fortement dégradé du Nord de la France},

author = {Julie Leclercq-Dransart and Céline Pernin and Brice Louvel and Sylvain Demuynck and Fabien Grumiaux and Christelle Pruvot and Francis Douay and Alain Leprêtre},

year  = {2014},

date = {2014-01-01},

booktitle = {Rever 5 : REVER et CONCILIER, 5ème journées atelier. 5-6 février 2014, Université de Rouen},

abstract = {Contexte l’ancien bassin minier du nord - pas de calais, et plus particulièrement le site atelier metaleurop (réseau safir http://www.safir-network.com/site_metaleurop.html), est un secteur constitué de paysages fortement dégradés et morcelés par les activités humaines (urbanisation, activités industrielles, agriculture intensive). la forte contamination des sols en plomb, cadmium et zinc aux alentours de l’ancienne fonderie de plomb metaleurop nord (noyelles-godault) est notoirement connue. elle conduit à de profonds dysfonctionnements des sols et les concentrations en métaux des productions agricoles végétales excèdent souvent les valeurs réglementaires en vigueur pour l’alimentation humaine, voire animale. l’enjeu majeur pour les institutions en charge de la gestion de ces espaces dégradés est de contribuer à leur restauration en vue de réduire d’une part, les dangers pour les populations et l’environnement et d’autre part, de favoriser leur renaturation et une reconquête paysagère. les services de l’etat envisagent de boiser les terres agricoles les plus contaminées pour les exclure de la production alimentaire. c’est une démarche qu’avait entreprise metaleurop nord entre 1997 et 2003, date de sa fermeture. ceci s’est traduit par le boisement d’environ 85 ha parmi les terres agricoles les plus contaminées aux alentours de l’ancienne fonderie. il est projeté d’exclure toute production agricole sur les sols présentant des concentrations en plomb supérieures à 500 mg kg-1. environ 50 ha de terres labourées pourraient changer d’usage et être boisées. objectif général en octobre 2012, un travail de thèse a débuté avec pour objectif de contribuer au développement du phytomanagement sur des sols fortement contaminés par des métaux. il s’agit de favoriser une renaturation et une biodiversité fonctionnelle des sols les plus contaminés en s’inspirant du retour d’expériences acquises sur les sols anciennement boisés. la fonctionnalité des sols sera observée à différentes échelles au regard de différents indicateurs physico-chimiques et biologiques. matériel et méthode plusieurs dispositifs expérimentaux sont mis en place en vue : •de comparer la vitesse de dégradation des matières organiques sur 4 parcelles boisées (mono/pluri espèces, amendements minéraux des sols ou non) à l’aide de 256 sacs de litière et suivi de la pédofaune ; •d’évaluer la restauration d’une connectivité écologique au travers de 6 modalités (bois raméal fragmenté, miscanthus, paille de céréale, feuilles mortes, bâche plastique et témoin) : inventaire mensuel de la pédofaune (grands groupes taxonomiques), suivi de la dégradation des matières organiques et des activités enzymatiques des sols… •d’examiner différents aménagements paysagers sur ce secteur dégradé à l’aide d’un panel d’indicateurs biologiques et physico-chimiques des sols. premiers résultats il a été mis en évidence : une mosaïque de paysages (plantations arborées, bois, cultures, zones résidentielles et d’activités économiques) entre lesquels la transition est souvent brutale ; des caractéristiques pédologiques variables notamment en termes de teneurs en carbonates, d’engorgement des sols par la nappe phréatique et de degré de contamination ; des communautés faunistiques perturbées, bien que la richesse spécifique de certains groupes soit élevée (carabes) ; et aussi l’absence de micro-habitats et de continuité écologique. les premiers résultats seront présentés et discutés.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Leclercq2014d,

title = {Évaluation de méthodes d’ingénierie écologique visant à restaurer les habitats dégradés: Intérêt du mulching dans la reconnexion écologique},

author = {Julie Leclercq-Dransart and Lucia Santorufo and Céline Pernin and Sylvain Demuynck and Brice Louvel and Fabien Grumiaux and Christelle Pruvot and Francis Douay and Alain Leprêtre},

year  = {2014},

date = {2014-01-01},

booktitle = {Gestion et requalification durable des sites et sols pollués : Expériences en Nord - Pas de Calais, 25-26 septembre 2014, Villeneuve d’Ascq},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Leclercq2014c,

title = {Le mulch de miscanthus peut-il présenter un intérêt pour la pédofaune dans un contexte dégradé et contaminé par les éléments traces métalliques?},

author = {Julie Leclercq-Dransart and Lucia Santorufo and Céline Pernin and Sylvain Demuynck and Brice Louvel and Fabien Grumiaux and Francis Douay and Alain Leprêtre},

year  = {2014},

date = {2014-01-01},

booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris},

abstract = {L’urbanisation, les activités industrielles, la pollution métallique, l’agriculture intensive sont les causes principales de la fragmentation et de la perturbation des sols dans l’ancien bassin minier de la région nord-pas de calais. ceux-ci induisent une perte de biodiversité et le fonctionnement du sol s’en trouve altéré. l’objectif de ce travail est d’évaluer l’intérêt du mulch de miscanthus x giganteus pour constituer un milieu attractif pour la pédofaune et favoriser la recolonisation des sols dégradés. en mars 2013, une expérimentation a été mise en place sur deux parcelles boisées. trois modalités de mulch (miscanthus, bois raméal fragmenté (brf), feuilles mortes) et un témoin (sol nu) ont été caractérisées en termes de concentrations en cadmium (cd), plomb (pb) et zinc (zn) ainsi que par les abondances moyennes et totales de la macrofaune. le mulch de miscanthus, bien que moins colonisé par la pédofaune par rapport aux feuilles ou au brf, est toutefois plus favorable qu’un sol nu. il présente des teneurs en cd, pb et zn très inférieures à celles du brf et des feuilles. le miscanthus apparaît alors comme une solution intermédiaire qui pourrait présenter un intérêt pour la pédofaune dans les réflexions de restauration de sols dégradés.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Santorufo2014,

title = {Linking litter characteristics and microbial communities in different stages of decomposition in fly ash amended soils},

author = {Lucia Santorufo and Julie Leclercq-Dransart and Brice Louvel and Francis Douay},

year  = {2014},

date = {2014-01-01},

booktitle = {First Global Soil Biodiversity Conference - Assessing soil biodiversity and its role for ecosystem services, 2-5 décembre 2014, Dijon},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Leclercq2014b,

title = {The use of indicators to characterize the functioning of soils strongly affected by metallurgical activities},

author = {Julie Leclercq-Dransart and Lucia Santorufo and Céline Pernin and Sylvain Demuynck and Brice Louvel and Fabien Grumiaux and Sébastien Détriché and Francis Douay and Alain Leprêtre},

year  = {2014},

date = {2014-01-01},

booktitle = {First Global Soil Biodiversity Conference - Assessing soil biodiversity and its role for ecosystem services, 2-5 décembre 2014, Dijon},

abstract = {The former coal mining field of northern france has been heavily disturbed due to human activities (urbanization, industry and intensive agriculture). this is particularly the case around a lead smelter (metaleurop nord) that emitted into the atmosphere until 2003 considerable quantities of metals. in order to restore this degraded area and to help reducing the environmental hazard, the most contaminated sites were afforested started in 1997. thus, 85 ha were planted around this former industrial plant creating a “green ring”. different tree species and different management options were established, but no study has been conducted on the effects of different tree species and managements on soil quality and functionality. the main aim of this study was to assess soil functionality of several parcels, differing by vegetation cover (species of trees, planting density, etc.), using soil physico-chemical and biological indicators. results would allow proposing a management for the restoration of these degraded environments. the study site consists of 12 plots: nine wooded plots (plantations of ash, poplar, maple or mixed plantations, with or without soil amendment) and three reference soils (agricultural, hedgerow, forest). the general status of trees and the truck diameters measurements were made. in addition, the topsoil was characterized by texture, bulk density, ph, cec, trace metal concentrations, etc. several soil biological indicators belonging to different functional and trophic groups were selected: microbial activity, ergosterol quantification (as estimation of fungal biomass), enzyme activities (laccase and urease) and several groups of pedofauna. indeed, the imagos emergence of soil insects was monitoring. it was conducted from mid-april to july 2014 using three soil emergence traps per plot.},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Leclercq2013,

title = {Ecologie et restauration de la biodiversité des sols perturbés dans un contexte de phytomanagement},

author = {Julie Leclercq-Dransart and Brice Louvel and Christelle Pruvot and Francis Douay},

year  = {2013},

date = {2013-01-01},

booktitle = {Journée de la recherche de l’UCL. 21 mai 2013, Lille},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Cebron2011a,

title = {Root exudates modify bacterial diversity of phenanthrene degraders in PAH-polluted but not phenanthrene degradation rates},

author = {Aurélie Cébron and Brice Louvel and Pierre Faure and Christian France-Lanord and Yin Chen and J.-Colin Murrell and Corinne Leyval},

year  = {2011},

date = {2011-01-01},

journal = {Environmental Microbiology},

volume = {13},

number = {3},

pages = {722-736},

abstract = {To determine whether the diversity of phenanthrenedegrading bacteria in an aged polycyclic aromatic hydrocarbon (pah) contaminated soil is affected by the addition of plant root exudates, dna stable isotope probing (sip) was used. microcosms of soil with and without addition of ryegrass exudates and with 13c-labelled phenanthrene (phe) were monitored over 12 days. phe degradation was slightly delayed in the presence of added exudate after 4 days of incubation. after 12 days, 68% of added phe disappeared both with and without exudate. carbon balance using isotopic analyses indicated that a part of the 13c-phe was not totally mineralized as 13co2 but unidentified 13c-compounds (i.e. 13c-phe or 13c-labelled metabolites) were trapped into the soil matrix. temporal thermal gradient gel electrophoresis (ttge) analyses of 16s rrna genes were performed on recovered 13c-enriched dna fractions. 16s rrna gene banding showed the impact of root exudates on diversity of phe-degrading bacteria. with phe as a fresh sole carbon source, pseudoxanthomonas sp. and microbacterium sp. were the major phe degraders, while in the presence of exudates, pseudomonas sp. and arthrobacter sp. were favoured. these two different phe-degrading bacterial populations were also distinguished through detection of pah-ring hydroxylating dioxygenase (pah-rhda) genes by real-time pcr. root exudates favoured the development of a higher diversity of bacteria and increased the abundance of bacteria containing known pahrhda genes.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Louvel2011,

title = {Root exudates affect phenanthrene biodegradation, bacterial community and functional gene expression in sand microcosms},

author = {Brice Louvel and Aurélie Cébron and Corinne Leyval},

year  = {2011},

date = {2011-01-01},

journal = {International Biodeterioration & Biodegradation},

volume = {65},

number = {7},

pages = {947-953},

abstract = {Polycyclic aromatic hydrocarbon (pah) degradation may be increased in plant rhizosphere, but the role of plants in pah degradation is not clearly understood. we investigated the effect of ryegrass root exudates on phenanthrene (phe) degradation, phe degrading bacterial population and functional gene expression in sand microcosms inoculated with bacteria from an industrial pah polluted soil. dominant bacteria were identified using 16s rdna based temporal thermal gradient electrophoresis (ttge). functional pah-ring hydroxylating dioxygenase (pah-rhdα) genes (dna) and transcripts (rna) were quantified through real-time pcr and rt-pcr. without root exudates phe degradation was rapid and complete within 5 days. herbaspirillum sp. and pseudomonas putida were the dominant strains. percentage of pah-rhdα relative to 16s rrna, both for genes and transcripts, was the highest after only two days and then decreased. with phenanthrene and root exudates as carbon sources, only 50% of the phe was degraded in 10 days. p. putida-related bacteria were also detected with other strains related to pseudomonas sp. and paenibacillus. phe degradation was reduced, and the percentage of pah-rhdα transcripts was lower with root exudates than with only phe, but increased over the incubation, while pah-rhdα gene copy number was higher},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}