Membre

@article{ridoskova:hal-04879845,

title = {Enhancing the prediction of arsenic bioavailability in soils with the diffusive gradient in thin film technique},

author = {Andrea Ridošková and Aurélie Pelfrêne and Pavlína Pelcová and Christophe Waterlot and Denisa Holasová and Michal Morávek},

url = {https://hal.science/hal-04879845},

doi = {10.1016/j.chemosphere.2024.144015},

year  = {2025},

date = {2025-02-01},

urldate = {2025-02-01},

journal = {Chemosphere},

volume = {371},

pages = {144015},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{oforiagyemang:hal-04362740,

title = {Plant testing with hemp and miscanthus to assess phytomanagement options including biostimulants and mycorrhizae on a metal-contaminated soil to provide biomass for sustainable biofuel production},

author = {Felix Ofori-Agyemang and Christophe Waterlot and James Manu and Roman Laloge and Romain Francin and Eleni Papazoglou and Efthymia Alexopoulou and Anissa Lounès-Hadj Sahraoui and Benoît Tisserant and Michel Mench and Aritz Burges and Nadège Oustrière},

url = {https://hal.science/hal-04362740},

doi = {10.1016/j.scitotenv.2023.169527},

year  = {2024},

date = {2024-02-01},

urldate = {2024-02-01},

journal = {Science of the Total Environment},

volume = {912},

pages = {169527},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{waterlot:hal-04403673,

title = {Sustainable pathways to oxicams via heterogeneous biosourced catalysts - Recyclable and reusable materials},

author = {Christophe Waterlot and Alina Ghinet and Pierrick Dufrénoy and Marie Hechelski and Adam Daïch and Damien Betrancourt and David Bulteel},

url = {https://hal.science/hal-04403673},

doi = {10.1016/j.jclepro.2024.140684},

year  = {2024},

date = {2024-01-01},

urldate = {2024-01-01},

journal = {Journal of Cleaner Production},

pages = {140684},

publisher = {Elsevier},

note = {ACL},

keywords = {ER2, ER4},

pubstate = {published},

tppubtype = {article}

}

@article{oforiagyemang:hal-04619295,

title = {Phytomanagement of a metal-contaminated agricultural soil with Sorghum bicolor, humic / fulvic acids and arbuscular mycorrhizal fungi near the former Pb/Zn metaleurop Nord smelter},

author = {Felix Ofori-Agyemang and Aritz Burges and Christophe Waterlot and Anissa Lounès andHadj Sahraoui and Benoît Tisserant and Michel Mench and Nadège Oustrière},

url = {https://hal.science/hal-04619295},

doi = {10.1016/j.chemosphere.2024.142624},

year  = {2024},

date = {2024-08-01},

urldate = {2024-08-01},

journal = {Chemosphere},

volume = {362},

pages = {142624},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{oconnor:hal-04633831,

title = {Young trees share soil water with wheat in an alley-cropping system in a wet crop year: Evidence from 2H2O artificial labeling},

author = {Claire O'Connor and Caroline Choma and Aichatou Ndiaye and François Delbende and Bernhard Zeller and Eric Manouvrier and Hélène Desmyttère and Ali Siah and Christophe Waterlot and Kasaina Sitraka Andrianarisoa},

url = {https://hal.science/hal-04633831},

doi = {10.1016/j.jhydrol.2024.131021},

year  = {2024},

date = {2024-05-01},

urldate = {2024-05-01},

journal = {Journal of Hydrology},

volume = {635},

pages = {131021},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{oconnor:hal-04457985,

title = {Tree–wheat vertical fine root distribution in a 4-year-old temperate alley-cropping system},

author = {Claire O'Connor and Caroline Choma and François Delbende and Bernhard Zeller and Eric Manouvrier and Hélène Desmyttère and Ali Siah and Christophe Waterlot and Kasaina Sitraka Andrianarisoa},

url = {https://hal.science/hal-04457985},

doi = {10.1007/s10457-023-00945-w},

year  = {2024},

date = {2024-01-01},

urldate = {2024-01-01},

journal = {Agroforestry Systems},

publisher = {Springer Verlag},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{oconnor:hal-04458013,

title = {Early share of 15N-labelled fertilizer between trees and crop in young temperate alley-cropping system},

author = {Claire O'Connor and Bernhard Zeller and Caroline Choma and François Delbende and Eric Manouvrier and Hélène Desmyttère and Ali Siah and Christophe Waterlot and Kasaina Sitraka Andrianarisoa},

url = {https://hal.science/hal-04458013},

doi = {10.1016/j.eja.2023.127013},

year  = {2024},

date = {2024-01-01},

urldate = {2024-01-01},

journal = {European Journal of Agronomy},

volume = {152},

pages = {pp. 127013},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{safi:hal-04767976,

title = {Cu-promoted Access to 1,4-Diazine-Fused Isoindoles Through Concomitant C sp 3 –N and C sp 2 –N Bonds Formation Starting from Constrained N,O -acetals},

author = {Christine Safi and Mohamed Othman and Ata Martin Lawson and Ján Moncol and Hassan Oulyadi and Sergiu Shova and Christophe Waterlot and Alina Ghinet and Adam Daïch},

url = {https://hal.science/hal-04767976},

doi = {10.1002/adsc.202400343},

year  = {2024},

date = {2024-10-01},

urldate = {2024-10-01},

journal = {Advanced Synthesis and Catalysis},

publisher = {Wiley-VCH Verlag},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{OConnor2023,

title = {Trees in temperate alley-cropping systems develop deep fine roots 

	5 years after plantation: What are the consequences on soil resources?},

author = {Claire O'Connor and Bernarhd Zeller and Caroline Choma and François Delbende and Ali Siah and Christophe Waterlot and Kasaina-Sitraka Andrianarisoa},

year  = {2023},

date = {2023-01-01},

journal = {Agriculture, Ecosystems & Environment},

volume = {345},

pages = {108339},

abstract = {Trees in alley-cropping systems (AC) were reported to develop deeper fine roots compared to forest trees and that they can modify soil water (SWC), mineral nitrogen (SMN) and organic matter (SOM) content. However, intercropping young trees has not been studied extensively. This study aimed to count tree fine roots abundance (TFRA) along a chronosequence of AC stands, to determine factors explaining its variability and to highlight its effects on soil resources. Seventeen alley-cropping plots ranging from 3 to 12 years old were chosen on farms in northern France. TFRA was measured by the core break method using soil samples collected at 0, 1, 3 and 10 m from a referent tree (a maple, a hybrid walnut or a hornbeam) down to 2 m depth. Before four years old, tree fine roots colonized the topsoil (0–30 cm) in rows and then mainly grew vertically from 4 to 6 years old, before laterally exploring deep soil layer (1–2 m) beyond this age. Stepwise analyses showed that stand age, tillage frequency and crop rotation duration explained 60 % of the variability of the sum of TFRA calculated for all soil layers at all distances from the tree row. The SWC was negatively correlated to TFRA suggesting that as trees get older, they dried the deep soil layer below the crop rooting zone and increased the soil depth able to store autumn and winter rainfall. No significant effect of either stand age or distance from tree rows was observed for SMN. It varied significantly with soil depth (R² = 0.3***) and was strongly correlated with soil nitrate content (R² = 0.97***). The soil ammonium content was significantly correlated with TFRA, suggesting that tree fine roots favor ammonium production or accumulation in soil, which may potentially allow for a reduction in the mineral nitrogen (N) mobility for leaching. Finally, we found a significantly high SOM correlated with TFRA only in topsoil on the tree rows at our oldest stands. No change of SOM was observed in the deep soil layer regardless of stand age. From this study, we concluded that fine root plasticity of intercropped trees occurred at early stage and may contribute with age to a better use of soil water, to managing the soil mineral N dynamic and to sequestrating carbon, at least in tree rows.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Paguet2023,

title = {Multivariate analysis of chemical and genetic diversity of wild Humulus 

	lupulus L. (hop) collected in situ in northern France},

author = {Anne-Sophie Paguet and Ali Siah and Gabriel Lefèvre and Sophie Moureu and Thierry Cadalen and Jennifer Samaillie and Franck Michels and Barbara Deracinois and Christophe Flahaut and Harmony Alves-Dos-Santos and Audrey Etienne-Debaecker and Caroline Rambaud and Sylvie Chollet and Roland Molinié and Jean-Xavier Fontaine and Christophe Waterlot and Marie-Laure Fauconnier and Sevser Sahpaz and Céline Rivière},

year  = {2023},

date = {2023-01-01},

journal = {Phytochemistry},

volume = {225},

pages = {113508},

abstract = {Highlights •50 wild hops from the North of France were genetically and chemically characterized. •Microsatellite-based study underlined high polymorphism among wild hop accessions. •Volatile compound analysis showed minor but original metabolites in wild hops. •Wild hops differ from commercial varieties in their high β-acid content and metabolomic diversity. •Multifactorial analysis suggested a potential terroir effect to explain this chemodiversity. The hop plant (Humulus lupulus L.) has been exploited for a long time for both its brewing and medicinal uses, due in particular to its specific chemical composition. These last years, hop cultivation that was in decline has been experiencing a renewal for several reasons, such as a craze for strongly hopped aromatic beers. In this context, the present work aims at investigating the genetic and chemical diversity of fifty wild hops collected from different locations in Northern France. These wild hops were compared to ten commercial varieties and three heirloom varieties cultivated in the same sampled geographical area. Genetic analysis relying on genome fingerprinting using 11 microsatellite markers showed a high level of diversity. A total of 56 alleles were determined with an average of 10.9 alleles per locus and assessed a significant population structure (mean pairwise FST = 0.29). Phytochemical characterization of hops was based on volatile compound analysis by HS-SPME GC-MS, quantification of the main prenylated phenolic compounds by UHPLC-UV as well as untargeted metabolomics by UHPLC-HRMS and revealed a high level of chemical diversity among the assessed wild accessions. In particular, analysis of volatile compounds revealed the presence of some minor but original compounds, such as aromadendrene, allo-aromadendrene, isoledene, β-guaiene, α-ylangene and β-pinene in some wild accessions; while analysis of phenolic compounds showed high content of β-acids in these wild accessions, up to 2.37% of colupulone. Genetic diversity of wild hops previously observed was hence supported by their chemical diversity. Sample soil analysis was also performed to get a pedological classification of these different collection sites. Results of the multivariate statistical analysis suggest that wild hops constitute a huge pool of chemical and genetic diversity of this species.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Vuillemin2023,

title = {Copper-uptake mediated by an ecofriendly zwitterionic ionic liquid: 

	A new challenge for a cleaner bioeconomy},

author = {Marie-E. Vuillemin and Christophe Waterlot and Anthony Verdin and Sylvain Laclef and Christine Cézard and David Lesur and Catherine Sarazin and Dominique Courcot and Caroline Hadad and Eric Husson and Albert-Nguyen Van-Nhien},

year  = {2023},

date = {2023-01-01},

journal = {Journal of Environmental Sciences},

volume = {130},

pages = {92-101},

abstract = {This study aims to investigate the ability of an imidazolium biobased Zwitterionic Ionic Liquids (ZILs) in enhancing the phytoavailability of copper from garden (G) and vineyard (V) soils using the model plant ryegrass. Uncontaminated and artificially contaminated CuSO4 soils, unamended and ZIL-amended soil modalities were designed. The copper/ZIL molar ratio (1/4) introduced was rationally established based on molecular modeling and on the maximal copper concentration in artificially contaminated soil. Higher accumulation of copper in the shoots was detected for the uncontaminated and copper contaminated ZIL amended V soils (18.9 and 23.3 mg.kg−1, respectively) contrary to G soils together with a ZIL concentration of around 3% w/w detected by LC-MS analyses. These data evidenced a Cu-accumulation improvement of 38 and 66% compared to non-amended V soils (13.6 and 13.9 mg.kg−1 respectively). ZIL would be mainly present under Cu(II)-ZIL4 complexes in the shoots. The impact on the chemical composition of shoot were also studied. The results show that depending on the soils modalitity, the presence of free copper and/or ZIL led to different chemical compositions in lignin and monomeric sugar contents. In the biorefinery context, performances of enzymatic hydrolysis of shoots were also related to the presence of both ZIL and copper under free or complex forms. Ecotoxicity assessment of the vineyard soil samples indicated that the quantity of copper and ZIL remaining in the soils had no significant toxicity. ZIL amendment in a copper-contaminated soil was demonstrated as being a promising way to promote the valorization of phytoremediation plants.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{oconnor:hal-04288814,

title = {Trees in temperate alley-cropping systems develop deep fine roots 5 years after plantation: What are the consequences on soil resources?},

author = {Claire O'Connor and Bernarhd Zeller and Caroline Choma and François Delbende and Ali Siah and Christophe Waterlot and Kasaina Sitraka Andrianarisoa},

url = {https://hal.inrae.fr/hal-04288814},

doi = {10.1016/j.agee.2022.108339},

year  = {2023},

date = {2023-04-01},

urldate = {2023-04-01},

journal = {Agriculture, Ecosystems & Environment},

volume = {345},

pages = {108339},

publisher = {Elsevier},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{hechelski:hal-04633986,

title = {Influence of P-fertilizers on photosynthetic pigments and phytohormones of perennial ryegrass grown on co-contaminated soils},

author = {Marie Hechelski and Brice Louvel and Christophe Waterlot},

url = {https://hal.science/hal-04633986},

doi = {10.1007/s10725-023-01088-9},

year  = {2023},

date = {2023-11-01},

urldate = {2023-11-01},

journal = {Plant Growth Regulation},

volume = {102},

number = {3},

pages = {659-668},

publisher = {Springer Verlag},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{guerin:hal-03771356,

title = {The phytoextraction power of Cichorium intybus L. on metal-contaminated soil: Focus on time- and cultivar-depending accumulation and distribution of cadmium, lead and zinc},

author = {Théo Guérin and Alina Ghinet and Christophe Waterlot},

url = {https://hal.archives-ouvertes.fr/hal-03771356},

doi = {10.1016/j.chemosphere.2021.132122},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

journal = {Chemosphere},

volume = {287},

pages = {132122},

publisher = {Elsevier},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{luyckx:hal-03851601b,

title = {Impact of Silicon and Heavy Metals on Hemp (Cannabis sativa L.) Bast Fibres Properties: An Industrial and Agricultural Perspective},

author = {Marie Luyckx and Mathilde Blanquet and Arnaud Isenborghs and Gea Guerriero and Géraldine Bidar and Christophe Waterlot and Francis Douay and Stanley Lutts},

url = {https://hal.archives-ouvertes.fr/hal-03851601},

doi = {10.1007/s41742-022-00446-1},

year  = {2022},

date = {2022-10-01},

urldate = {2022-10-01},

journal = {International Journal of Environmental Research},

volume = {16},

number = {5},

pages = {82},

publisher = {Springer Verlag},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Dufrenoy2022,

title = {Green synthesis of a new series of pyroglutamides targeting human 

	farnesyltransferase},

author = {Pierrick Dufrénoy and Marie Hechelski and Adam Daïch and Amaury Farce and Emmanuelle Lipka and Joëlle Dubois and Christophe Waterlot and Alina Ghinet},

year  = {2022},

date = {2022-01-01},

journal = {Sustainable Chemistry and Pharmacy},

volume = {30},

pages = {100894},

abstract = {Pyroglutamide derivatives have emerged as promising inhibitors of human farnesyltransferase (FTIs), an important target in oncology and also in rare diseases such as Hutchinson-Gilford progeria syndrome (HGPS). This report describes the chemical efforts to enrich the pyroglutamide series using greener and recyclable catalysts. The central reaction studied was an amidation between methyl pyroglutamates or vinylogues and amines. Ten catalysts have been tested in this amidation reaction: two classical Lewis acids (ZnCl2, ZrCl4), four impregnated montmorillonite K10 with ZnCl2 namely Cat1, Cat2, Cat3 and Cat4 (not activated, activated at 120 °C, 280 °C and 500 °C, respectively) and four montmorillonites K10 (commercial montmorillonite K10 not activated, activated at 120 °C, 280 °C and 500 °C). The most efficient catalyst was Cat4. The recyclability of Cat4 over five synthesis runs has been successfully tested. Twenty-six amides were synthesized and screened for their potential to inhibit human farnesyltransferase. Four points of chemical modulation around the pyrrolidine-2-one ring have been realized allowing to complete structure-activity relationships in these series. The study revealed several potent inhibitors targeting human farnesyltransferase in vitro with IC50 values in the submicromolar range and down to 30 nM. The docking of compounds in the active site of FTase highlighted that the S-isomers of pyroglutamides had good affinity. This study propels pyroglutamide derivatives as promising candidates for future functionality assays and in vivo evaluation.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Janus2022,

title = {Synthesis of bio-sourced catalysts from ryegrass produced on the 

	metallurgical sites for the synthesis of therapeutic molecules: a 

	possible option? Feedback from the NEW-REGENERATIS project},

author = {Adeline Janus and Alina Ghinet and Christophe Waterlot},

year  = {2022},

date = {2022-01-01},

booktitle = {International Conference on Soils, Sediments and water (InterSoil'2022), 

	5-6 October, Bruxelles (Belgium)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Schnackenberg2022,

title = {Effects of amendments on the mobility, bioaccessibility, and phytoavailability of As, Cd, Pb, and Zn in contaminated urban kitchen garden soils},

author = {Ashley Schnackenberg and Géraldine Bidar and Valérie Bert and Patrice Cannavo and Sébastien Détriché and Francis Douay and René Guenon and Liliane Jean-Soro and Alice Kohli and Thierry Lebeau and Karen Perronnet and Laure Vidal-Beaudet and Christophe Waterlot and Aurélie Pelfrêne},

year  = {2022},

date = {2022-01-01},

booktitle = {11th Conference on Soils of Urban, Industrial, Traffic and Mining Areas (SUITMA), 5-9 September 2022, Berlin (Germany)},

abstract = {Urban gardening is a growing trend that responds to the need for nature in cities, a growing movement towards healthy and sustainable eating and food production, and difficult economic and societal contexts. Both private and community gardens in these areas are often on urban wastelands, along roads, highways, or railways, near industrial sites, and have even sometimes earned the term “guerrilla gardens” for their lack of agronomic regulation. In France, many urban gardens are associated with varying levels of inorganic contaminants hailing from anthropogenic activity or geogenic metal(loid) presence. Moderately metal(loid)- contaminated urban kitchen garden soils can benefit from gentle remediation options including the use of soil amendments, which are able to improve soil function and agronomic quality while decreasing environmental and human health risk. This study first analyses the effects of common doses of a various commercialized soil amendments on the mobility of metalloid(s) in contaminated urban kitchen garden soils. Fourteen different amendments and amendment mixes were tested on three kitchen garden soils with diverse sources of anthropogenic or geogenous contamination and varying physico-chemical characteristics. Amendments from four differing categories were tested, including i) organic amendments like composts, ii) natural fertilizers such as bone meal, crushed horn, and organic poultry manure fertilizer, iii) calcareous amendments including two different limes, and iv) natural siliceous and alumino-silicate amendments, including diatomaceous earth and zeolite. Amendments at varying common doses were evaluated via chemical extractions on their ability to reduce the mobility of metallic elements after multiple weeks of maturation at a constant humidity and temperature. The most pertinent of these amendments were then chosen to be tested in conjunction with a vegetal model grown upon the amended soils in greenhouse conditions. 05.09.– 09.09 2022 Berlin, Germany Changes in physico-chemical soil characteristics, mobility and bioaccessibility of metal(loid)s in the soil, and phytoavailability in the lettuce were then tested. Mobility and bioaccessible metal(loid)s were evaluated with 0.05 M EDTA buffered at a pH of 7, an ammonium nitrate extraction, a 4-phase sequential extraction, and a UBM bioaccessibility test. Phytoavailability was determined using pseudo-total soil and plant metal(loid) contents. The study results show that it is possible to reduce both extractable and phytoavailable metal(oid)s with amendment addition. However, variable effects are seen according to the contaminated soil tested, and differences arise in the presence of a plant model.},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Vuillemin2022,

title = {Combining Phytoremediation and Biorefinery Strategies Assisted by 

	an Ecofriendly Zwitterionic Ionic Liquid: A New Challenge for a Cleaner 

	Bioeconomy},

author = {Marie Vuillemin and Christophe Waterlot and Anthony Verdin and Sylvain Laclef and Christine Cézard and David Lesur and Catherine Sarazin and Dominique Courcot and Caroline Hadad and Eric Husson and Albert Nguyen-van-Nhien},

year  = {2022},

date = {2022-01-01},

journal = {SSRN},

pages = {27pp},

abstract = {This study aims to investigate the ability of an imidazolium biobased Zwitterionic Ionic Liquids (ZILs) in enhancing the phytoextraction of copper from garden and vineyard soils using the model plant ryegrass (Lolium perenne L.). The copper/ZIL molar ratio (1/4) introduced was rationally established based on molecular modeling and on the maximal copper concentration in artificially contaminated soil. Higher accumulation of copper in the shoots was detected for the uncontaminated and copper contaminated ZIL amended soils (18.9 and 23.3 mg.kg-1, respectively) together with a similar ZIL concentration of around 3% w/w detected by LC-MS analyses. These data evidenced a phytoextraction improvement of 38 and 66% compared to non-amended soils. ZIL would be mainly present under Cu(II)-ZIL4 complexes in the shoots. However depending on the soil modalities, the presence of free copper and/or free ZIL led to different chemical compositions in lignin and monomeric sugar contents. In the biorefinery context, performances of enzymatic hydrolysis of shoots were related to the presence of both ZIL and copper under free or complex forms. Ecotoxicity of the different soils indicated that the quantity of copper and ZIL remaining in the soils had no significant toxicity. ZIL amendment in a copper-contaminated soil was demonstrated as being a promising way to promote the valorization of phytoremediation plants.},

note = {ACLO},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Waterlot2022,

title = {Biomass valorization},

author = {Christophe Waterlot},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

booktitle = {Current challenges of environmental contamination & its impact on  the ecosystems and human health, 4-5 juillet, Lille},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Ofori-Agyeman2022,

title = {Optimization of phytomanagement strategies on soils contaminated with metals (Cd, Pb, Zn) to provide biomass for clean biofuel production – Experience from a pot trial},

author = {Felix Ofori-Agyemang and Christophe Waterlot and Michel Mench and Nadège Oustrière},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

booktitle = {21st International Conference- Exhibition on Soils, Sediments and Water (INTERSOL), 21-23 June, Lyon},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Ofori-Agyeman2022a,

title = {Optimizing phytomanagement strategies on a metal (Cd, Pb, Zn, and 	Cu)-contaminated soil to provide biomass for clean biofuel production – progress from pot trial to Cd and Zn mixture},

author = {Felix Ofori-Agyemang and Christophe Waterlot and Michel Mench and Nadège Oustrière},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

booktitle = {8th European Bioremediation Conference, 12-17 June, Chania (Greece)},

pages = {224},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{schnackenberg:hal-03583334,

title = {Effects of Inorganic and Organic Amendments on the Predicted Bioavailability of As, Cd, Pb and Zn in Kitchen Garden Soils},

author = {Ashley Schnackenberg and Géraldine Bidar and Valérie Bert and Patrice Cannavo and Sébastien Détriché and Francis Douay and René Guenon and Liliane Jean-Soro and Alice Kohli and Thierry Lebeau and Karen Perronnet and Laure Vidal-Beaudet and Christophe Waterlot and Aurélie Pelfrêne},

url = {https://hal.archives-ouvertes.fr/hal-03583334},

doi = {10.21926/aeer.2201004},

year  = {2022},

date = {2022-02-01},

urldate = {2022-02-01},

journal = {Advances in Environmental and Engineering Research},

volume = {3},

pages = {1 - 1},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Guerin2022,

title = {Removal of heavy metals from contaminated water using industrial 	wastes containing calcium and magnesium},

author = {Théo Guérin and Nadège Oustrière and David Bulteel and Damien Betrancourt and Alina Ghinet and Sandhya Malladi and Justice-G. Kaleo-Bioh and Amaury Blanc-Brude and Abraham Pappoe and Christophe Waterlot},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

journal = {Journal of Cleaner Production},

volume = {337},

pages = {130472},

abstract = {Two industrial wastes (IW1 and IW2) were investigated as potential sorbents to retain cadmium and lead from contaminated water. The sorption experiments have been conducted through lab-engineered cartridge filtration system in order to get as close as possible to industrial processes. The effectiveness of IW1 and IW2 were compared to that of activated charcoal Norit® (AC), the best-known matrix for its excellent retention capacity. The sorption isotherms of metals on the three solid sorbents (IW1, IW2 and AC) were built, and then mathematically modelled. Free Gibbs energy (ΔG°) of the sorption processes as well as the equilibrium parameter (RL) have been calculated for each pollutant-sorbent couple. The study revealed that: i) for cadmium sorption, IW2 was much more effective than IW1 and especially than AC (18-fold higher in term of maximal sorption capacity); ii) if IW2 was slightly less effective than AC to retain lead, it can be still considered as an interesting sorbent due to its low cost; iii) the sorption of cadmium was as spontaneous on AC as on IW1, and almost half as much on IW2; iv) regarding lead retention, the sorption on the three sorbents was spontaneous; (v) the retention of cadmium and lead was mainly explained by precipitation since otavite, cerussite and hydrocerussite were identified and characterized by X-ray diffraction of used sorbents. The study showed that the industrial wastes studied stood out as new efficient materials with sorption power equal to or greater than the reference material AC. The prospect of a new generation of industrial wastes with lasting efficiency as contaminated water depollution agents comes at a key moment in the search for new perspectives in the circular economy.},

note = {ACL},

keywords = {ER2, ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Hechelski2021,

title = {Biomass of ryegrass from field experiments: toward a cost-effective and efficient biosourced catalyst for the synthesis of Moclobemide},

author = {Marie Hechelski and Christophe Waterlot and Pierrick Dufrénoy and Brice Louvel and Adam Daïch and Alina Ghinet},

year  = {2021},

date = {2021-01-01},

journal = {Green Chemistry Letters and Reviews},

volume = {14},

number = {1},

pages = {13-20},

abstract = {Lolium perenne L., a common plant perennial ryegrass from Europe, 

	northern Africa and Asia, was selected to produce biomass under field 

	conditions. The biomass of this nonfood crop was from a highly contaminated 

	agricultural soil by potentially toxic metals (Cd, Pb and Zn) with 

	the aim of maintaining the agricultural vocation of these soils. 

	In this perspective and in view of the metal concentration (Cd, Pb, 

	Zn, macro- and micro-nutrients), the biomass of ryegrass was considered 

	as a bio ’ore’ resource and was used as the starting material for 

	the preparation of contemporary biosourced catalysts. The heterogeneous 

	catalyst from the current work was successfully used in the synthesis 

	of Moclobemide, used to treat anxiety and major depressive episodes. 

	Through this original approach, Moclobemide was synthesized in one 

	step, in 80% yield under solvent-free conditions.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{cornu2021advantages,

title = {Advantages and limits to copper phytoextraction in vineyards},

author = {Jean-Yves Cornu and Christophe Waterlot and Thierry Lebeau},

doi = {https://doi.org/10.1007/s11356-021-13450-3},

year  = {2021},

date = {2021-01-01},

journal = {Environmental Science and Pollution Research},

pages = {1--10},

publisher = {Springer},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{su13010034,

title = {Toward a New Way for the Valorization of Miscanthus Biomass Produced on Metal-Contaminated Soils Part 2: Miscanthus-Based Biosourced Catalyst: Design, Preparation, and Catalytic Efficiency in the Synthesis of Moclobemide},

author = {Théo Guérin and Alina Ghinet and Christophe Waterlot},

url = {https://www.mdpi.com/2071-1050/13/1/34},

doi = {10.3390/su13010034},

issn = {2071-1050},

year  = {2021},

date = {2021-01-01},

journal = {Sustainability},

volume = {13},

number = {1},

abstract = {The conception of two biosourced catalysts (biocatalysts) using stems of miscanthus from the first part of this study are described herein. The temperature and the process used to extract metals from plant as mixture of Lewis acids were investigated in detail and proved to be essential in the design of the biosourced catalysts and their catalytic efficiency. One part of the crude mixture of Lewis acids extracted from the aerial parts of miscanthus plants was used without further treatment as a homogeneous biocatalyst (M1), and the other part was supported on montmorillonite K10 to provide a heterogeneous biocatalyst (MM1). M1 and MM1 were next tested in the synthesis of moclobemide (main ingredient of a drug used to treat depression) and led to excellent yield. Additional comparative experiments with different commercial metallic salts (NaCl, KCl, CaCl2, MgCl2, CuCl2, ZnCl2, FeCl2, FeCl3, MnCl2, and AlCl3) and their mixtures were carried out and underlined the importance of the multimetallic synergy on catalytic activity. Finally, a comparison of this new synthetic method assisted by the biosourced catalyst with the previously described procedures to access moclobemide was realized by calculating their green chemistry metrics. This study revealed that the use of the biosourced catalyst led to one of the greenest synthetic methods described today to produce moclobemide.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{doi:10.1080/17518253.2020.1856943,

title = {Biomass of ryegrass from field experiments: toward a cost-effective and efficient biosourced catalyst for the synthesis of Moclobemide},

author = {Marie Hechelski and Christophe Waterlot and Pierrick Dufrénoy and Brice Louvel and Adam Daïch and Alina Ghinet},

url = {https://doi.org/10.1080/17518253.2020.1856943},

doi = {10.1080/17518253.2020.1856943},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Green Chemistry Letters and Reviews},

volume = {14},

number = {1},

pages = {15-22},

publisher = {Taylor & Francis},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{NSANGANWIMANA2021112125,

title = {Potentials of Miscanthus x giganteus for phytostabilization of trace element-contaminated soils: Ex situ experiment},

author = {Florien Nsanganwimana and Karim Suhail Al Souki and Christophe Waterlot and Francis Douay and Aurélie Pelfrêne and Andrea Ridošková and Brice Louvel and Bertrand Pourrut},

url = {https://www.sciencedirect.com/science/article/pii/S0147651321002360},

doi = {https://doi.org/10.1016/j.ecoenv.2021.112125},

issn = {0147-6513},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {214},

pages = {112125},

abstract = {Phytomanagement is proposed as a cost-effective and environmentally-friendly suggestion for sustainable use of large metal-contaminated areas. In the current work, the energy crop miscanthus (Miscanthus × giganteus) was grown in ex situ conditions on agricultural soils presenting a Cd, Pb and Zn contamination gradient. After 93 days of culture, shoot and root growth parameters were measured. Soils and plants were sampled as well to study the TE accumulation in miscanthus and the effects of this plant on TE mobility in soils. Results demonstrated that miscanthus growth depended more on the soils silt content rather than TE-contamination level. Moreover, soil organic carbon at T93 increased in the soils after miscanthus cultivation by 25.5–45.3%, whereas CaCl2-extractible TEs decreased due to complex rhizosphere processes driving plant mineral uptake, and organic carbon inputs into the rhizosphere. In the contaminated soils, miscanthus accumulated Cd, Pb and Zn mainly in roots (BCF in roots: Cd " Zn > Pb), while strongly reducing the transfer of these elements from soil to all organs and from roots to rhizomes, stems and leaves (average TFs: 0.01–0.06, 0.11–1.15 and 0.09–0.79 corresponding to Cd, Pb and Zn respectively). Therefore, miscanthus could be considered a TE-excluder, hence a potential candidate crop for coupling phytostabilization and biomass production on the studied Metaleurop TE-contaminated soils.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@inbook{10.1007/978-3-030-51210-1_92,

title = {Histopathological Changes in the Hepatopancreas of Porcellio Laevis (Crustacea, Isopoda) After Exposure to Cd and Zn Mixture},

author = {Chedliya Ghemari and Raja Jelassi and Hajer Khemaissia and Christophe Waterlot and Maryline Raimond and Catherine Souty-Grosset and Francis Douay and Karima Nasri-Ammar},

editor = {Mohamed Ksibi and Achraf Ghorbal and Sudip Chakraborty and Helder I. Chaminé and Maurizio Barbieri and Giulia Guerriero and Olfa Hentati and Abdelazim Negm and Anthony Lehmann and Jörg Römbke and Armando Costa Duarte and Elena Xoplaki and Nabil Khélifi and Gilles Colinet and Jo ao Miguel Dias and Imed Gargouri and Eric D. Van Hullebusch and Benigno Sánchez Cabrero and Settimio Ferlisi and Chedly Tizaoui and Amjad Kallel and Sami Rtimi and Sandeep Panda and Philippe Michaud and Jaya Narayana Sahu and Mongi Seffen and Vincenzo Naddeo},

doi = {https://doi.org/10.1007/978-3-030-51210-1_92},

isbn = {978-3-030-51210-1},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

booktitle = {Recent Advances in Environmental Science from the Euro-Mediterranean and Surrounding Regions (2nd Edition)},

pages = {587--592},

publisher = {Springer International Publishing},

address = {Cham},

abstract = {The ultrastructural changes on the hepatopancreas of Porcellio laevis individuals were investigated after their exposure to a mixture of cadmium (Cd) and zinc (Zn) during four weeks. At the end of the experiment, trace element (TE) concentrations were determined using atomic absorption spectrometry (AAS). The growth of individuals was calculated and compared among the treatments. From the first week until the end of the experiment, a weight gain was observed in P. laevis individuals. Additionally, the TE concentrations in the hepatopancreas and in the rest of the body were considerable and seem to be dose dependent. Moreover, our results confirmed the role of the hepatopancreas as the main storage organ. Thus, the conducted transmission electron microscopy highlighted remarkable alterations on the hepatopancreas cells resulting from the Cd and Zn bioaccumulation. The displayed alterations are principally the disorganization of the S- and B-cells leading to a destruction of the microvilli border, an increase of the lipid droplets and mitochondria as well as the appearance of trace elements in the form of B-type granules. Based on the obtained results herein, we can conclude that Porcellio laevis might be a good candidate in the prevention of TE pollution in the terrestrial ecosystems.},

note = {OS},

keywords = {ER4},

pubstate = {published},

tppubtype = {inbook}

}

@article{guerin:hal-03772849,

title = {Ecocatalysed Hurtley reaction: Synthesis of urolithin derivatives as new potential RAGE antagonists with anti-ageing properties},

author = {Théo Guérin and Christophe Waterlot and Emmanuelle Lipka and Philippe Gervois and David Bulteel and Damien Betrancourt and Carla Moignard and Adrian Sorin Nica and Christophe Furman and Alina Ghinet},

url = {https://hal.science/hal-03772849},

doi = {10.1016/j.scp.2021.100518},

year  = {2021},

date = {2021-10-01},

urldate = {2021-10-01},

journal = {Sustainable Chemistry and Pharmacy},

volume = {23},

pages = {100518},

publisher = {ELSEVIER},

note = {ACL},

keywords = {ER2, ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Ghemari2020,

title = {Physiological and histopathological responses of Porcellio laevis (Isopoda, Crustacea) as indicators of metal trace element contamination},

author = {Chedliya Ghemari and Raja Jelassi and Hajer Khemaissia and Christophe Waterlot and Maryline Raimond and Catherine Souty-Grosset and Francis Douay and Karima Nasri-Ammar},

year  = {2020},

date = {2020-01-01},

journal = {Microscopy Research and Technique},

volume = {83},

number = {4},

pages = {402-409},

abstract = {Abstract This study was designed to assess the impact of the mixture 

	of cadmium (Cd) and zinc (Zn) on the bioaccumulation and the ultrastructural 

	changes in the hepatopancreas of Porcellio laevis (Latreille, 1804) 

	after 4?weeks of exposure to contaminated Quercus leaves under laboratory 

	conditions. For each metal, four concentrations were used with four 

	replicates for each concentration. Metal concentrations in the hepatopancreas 

	and the rest of the body were determined using atomic absorption 

	spectrometry. From the first week until the end of the experiment, 

	a weight gain in P. laevis was observed particularly between the 

	first and the end of exposure from 93.3?±?18.22?mg fw to 105.22?±?16.16?mg 

	fw and from 106.4?±?22.67?mg fw to 125.9?±?23.9 mg fw for Mix1 

	and Mix4, respectively. Additionally, the determined metal trace 

	elements (MTE) concentrations in the hepatopancreas were considerably 

	higher compared to those in the rest of the body and seem to be dose-dependent. 

	Using transmission electron microscopy (TEM), some alterations were 

	highlighted in the hepatopancreas. The main observed alterations 

	were (a) the destruction of the microvilli border in a considerable 

	portion of cells, (b) the increase of the lipid droplets with different 

	shapes and sizes, (c) the increase in the number of the mitochondria, 

	and (d) the appearance of TE in the form of B-type granules. The 

	obtained results confirmed the ability of P. laevis to deal with 

	high amounts of MTE, suggesting its possible use in future soil's 

	biomonitoring programs.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Janus2020,

title = {Ex situ evaluation of the effects of biochars on environmental and toxicological availabilities of metals and polycyclic aromatic hydrocarbons},

author = {Adeline Janus and Christophe Waterlot and Francis Douay and Aurélie Pelfrêne},

year  = {2020},

date = {2020-01-01},

journal = {Environmental Science and Pollution Research},

volume = {27},

pages = {1852–1869},

abstract = {The present study experimented five biochars, one made from wood (400 

	°C, 12 h) and four made from miscanthus cultivated on contaminated 

	soils (temperature 400/600 °C, duration 45/90 min). They were used 

	as amendments at a 2% application rate on soil, cultivated or not 

	cultivated with ryegrass, contaminated with (i) metals (Cd, Pb, and 

	Zn), (ii) eight polycyclic aromatic hydrocarbons (PAHs), and (iii) 

	a mix of metals and PAHs. The objectives were (i) to compare the 

	effectiveness of the five biochars on soil parameters and pollutant 

	availability and (ii) to determine the influence of soil multicontamination 

	and ryegrass cultivation on biochar effectiveness. The results showed 

	that biochar application did not necessarily lead to lower pollutant 

	extractability and metal bioaccessibility. However, differences were 

	highlighted between the biochars. The miscanthus biochars produced 

	at 600 °C (BM600) showed higher effectiveness at decreasing metal 

	extractability than the miscanthus biochars produced at 400 °C (BM400) 

	due to its better sorption characteristics. In addition, ryegrass 

	cultivation did not impact pollutant availability but modified metal 

	bioaccessibility, especially for the soil amended with the BM600 

	and the woody biochar. Moreover, the presence of PAHs also negatively 

	impacted the metal bioaccessibility in the soil amended with the 

	BM600, and, on the contrary, positively impacted it in the soil amended 

	with the BM400. Complementary studies are therefore necessary to 

	understand the mechanisms involved, particularly in a context where 

	soils requiring remediation operations are often multicontaminated 

	and vegetated.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Bidar2020,

title = {Urban kitchen gardens: Effect of the soil contamination and parameters on the trace element accumulation in vegetables – A review},

author = {Géraldine Bidar and Aurélie Pelfrêne and Christophe Schwartz and Christophe Waterlot and Karin Sahmer and Franck Marot and Francis Douay},

year  = {2020},

date = {2020-01-01},

journal = {Science of the Total Environment},

volume = {738},

pages = {139569},

abstract = {Trace element contaminants in kitchen garden soils can contribute 

	to human exposure through the consumption of homegrown vegetables. 

	In urban areas, these soils can be contaminated to various degrees 

	by trace element (TE). They are characterized by a great variability 

	in their physicochemical parameters due to the high anthropization 

	level, the wide variety and combination of disturbance sources, aswell 

	as the diversity of cultivation practices and the large range of 

	contamination levels. Pollutants can be taken up by vegetables cultivated 

	in these soils and be concentrated in their edible parts. In this 

	review, the behavior of vegetables cultivated in contaminated kitchen 

	gardens is assessed through six examples of the most widely cultivated 

	vegetables (lettuce, tomato, bean, carrot, radish, potato). The role 

	of soil parameters that could influence the uptake of As, Cd, Cr, 

	Ni, Pb, and Zn by these vegetables is also discussed.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Bouchard2020,

title = {Etude des performance agroenvironnementales de différents outils d’aide à la decision (OAD) en fertilisation azotée et développement d’une méthode de pilotage dynamique},

author = {Marie-Astrid Bouchard and Herinaina-A.-L. Andriamandroso and Sitraka Andrianarisoa and Ali Siah and Christophe Waterlot and A Carlier and Sébastien Dandrifosse and C Guille and Bertrand Vandoorne},

year  = {2020},

date = {2020-01-01},

booktitle = {Phloème, 29-30 janvier 2020, Cité des Sciences & de l’Industrie, Paris},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Dufrenoy2020,

title = {New efficient eco-friendly supported catalysts for the synthesis of amides with antioxidant and anti-inflammatory properties},

author = {Pierrick Dufrénoy and Rogatien Charlet and Marie Hechelski and Adam Daïch and Christophe Waterlot and Samir Jawhara and Alina Ghinet},

year  = {2020},

date = {2020-01-01},

journal = {ChemMedChem},

volume = {15},

pages = {459-467},

abstract = {A new environmentally friendly approach for the synthesis of idrocilamide 

	(1), a marketed myorelaxant and anti-inflammatory agent, is reported 

	herein. The synthetic strategy involves a solvent-free aminolysis 

	reaction catalyzed by zinc-containing species (ZnCl2, montmorillonite 

	K10 (MK10) impregnated with ZnCl2 or eco-catalysts). The latter have 

	been prepared from the aerial parts of Lolium perenne L. plants grown 

	on contaminated soils from northern France without and with thermal 

	activation at 120 °C and supported on MK10 (Ecocat1 and Ecocat2, 

	respectively). The best aminolysis catalysts in the current study 

	(ZnCl2 and Ecocat2) were selected for additional aminolyses. Compared 

	to ZnCl2, Ecocat2 had the advantage of being reusable over five test 

	runs and constituted a sustainable catalyst allowing a green route 

	to idrocilamide. Synthesized derivatives 1–4, 6 and 9 were first 

	evaluated for their effect on reactive oxygen species (ROS) generation 

	from macrophages and displayed antioxidant properties by preventing 

	ROS production. Next, the analysis of the effect of molecules 1–4, 

	6 and 9 on macrophage migration between epithelial cells to human 

	opportunistic fungus Candida albicans indicated that molecules 2–4, 

	6 and 9 exert anti-inflammatory properties via reducing macrophage 

	migration while the parent idrocilamide (1) did not show any significant 

	effect. This work opens the way for the discovery of new analogues 

	of idrocilamide with improved properties.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Ghemari2020b,

title = {Bioaccumulation of heavy metals in the terrestrial isopod Porcellionides pruinosus in the vicinity of Gabes-Ghannouch industrial complex},

author = {Chedliya Ghemari and Christophe Waterlot and Anas Ayari and Francis Douay and Karima Nasri-Ammar},

year  = {2020},

date = {2020-01-01},

journal = {Human and Ecological Risk Assessment: An International Journal},

volume = {26},

number = {5},

pages = {1270-1284},

abstract = {The current study reports the effects of heavy metals issued from 

	the emissions of phosphate and their bioaccumulation in the soils, 

	leaf litter, and the terrestrial isopod Porcellionides pruinosus 

	in the vicinity of Gabes-Ghannouch industrial complex. Cd, Pb, Zn, 

	and Cu concentrations were measured in soils, leaf litter, and P. 

	pruinosus individuals, collected from four stations located at different 

	distances from the factory. Results showed that heavy metal concentrations 

	in soils varied from one site to another and did not reflect those 

	measured in individuals. In contrast, a positive correlation was 

	found between the isopods’ body length and the distance from the 

	pollution source, revealing the isopod sensitivity to the soil metal 

	concentrations. The bioaccumulation factor was calculated using soil 

	and leaf litter as a solid support with the aim of ordering the metal 

	accumulation in P. pruinosus for each site. Overall, P. pruinosus 

	could be defined as a macroconcentrator of Cd, Zn, and Cu (BAF>2) 

	and as a deconcentrator of Pb (BAF<1).},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Pelfrene2020,

title = {Evaluation of single-extraction methods to estimate the oral bioaccessibility of metal(loid)s in soils},

author = {Aurélie Pelfrêne and Karin Sahmer and Christophe Waterlot and Philippe Glorennec and Francis Douay and Barbara Le-Bot},

year  = {2020},

date = {2020-01-01},

journal = {Science of the Total Environment},

volume = {727},

pages = {138553},

abstract = {Incidental ingestion of polluted soil particles exposes the population 

	to toxic metal(loid)s. To refine the methods of exposure and risk 

	assessment, it is relevant to use bioaccessible concentrations of 

	metal(loid)s determined via in vitro digestion methods. However, 

	some validated methods are complex and costly, involving high technical 

	skills and numerous reagents. The objective of the present study 

	was to evaluate the suitability of four simple chemical extractions 

	to mimic the bioaccessible fraction of As, Cd, and Pb in the gastric 

	(G) and gastrointestinal (GI) phases obtained using the validated 

	UBM (unified bioaccessibility method) test. Acetic acid (0.11 M), 

	citric acid (0.11M), EDTA (0.16 M), and hydrochloric acid (HCl, 0.65%)were 

	separately tested in 201 soil sampleswith a wide range of physicochemical 

	parameters and metal(loid)s concentrations. Significant linear relationships 

	were observed with HCl, EDTA, and to a lesser extent with citric 

	acid. For the cheaper HCl method, correlations with the UBM ranged 

	from 0.91 to 0.99 for the G phase and from 0.72 to 0.97 for the GI 

	phase. This test can be used at least as a first-tier screening to 

	assess the oral bioaccessibility of As, Cd, and Pb.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Guerin2020,

title = {Wheat and ryegrass biomass ashes as effective sorbents for metallic and organic pollutants from contaminated water in lab-engineered cartridge filtration system},

author = {Théo Guérin and Alina Ghinet and Marc Hossarte and Christophe Waterlot},

year  = {2020},

date = {2020-01-01},

journal = {Bioresource Technology},

volume = {318},

pages = {124044},

abstract = {Three plant biomasses (miscanthus, ryegrass and wheat) have been considered 

	for the preparation of five different sorbents evaluated for their 

	potential to sorb cadmium and lead and four emergent organic compounds 

	(diclofenac, sulfamethoxazole, 17α-ethynylestradiol and triclosan) 

	from artificially contaminated water. Labcreated cartridges were 

	filled with each sorbent and all experiments were systematically 

	compared to activated charcoal Norit®. Results from activated charcoal, 

	wheat straw and acidified wheat straw were supported by the Langmuir 

	and Freundlich models. Wheat straw ashes were an excellent metal 

	extractor that exceeded the potential of well-known activated charcoal. 

	Acidified sorbents (wheat and ryegrass) were very effective in eliminating 

	the selected emerging organic contaminants displaying equipotent 

	or superior activity compared to activated charcoal. These results 

	open the way for further in natura studies by proposing new biosource 

	materials as new effective tools in the fight against water pollution.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Hechelski2020,

title = {Toward a new way for the valorization of miscanthus biomass produced on metal-contaminated soils Part 1: Mesocosm and field experiments},

author = {Marie Hechelski and Brice Louvel and Pierrick Dufrénoy and Alina Ghinet and Christophe Waterlot},

year  = {2020},

date = {2020-01-01},

journal = {Sustainability},

volume = {12},

pages = {9370},

abstract = {The effects of P-fertilizers (mono- and di-calcium phosphates) on 

	the bioavailability of metals and nutrients in leaves and stems of 

	Miscanthus × giganteus were studied in mesocosm and field experiments 

	in order to propose a new way for the valorization of miscanthus 

	biomass. The concentration of potentially toxic elements was generally 

	higher in stems than in leaves. Although P-fertilizers were added 

	to contaminated soils under sustainable conditions (from 0.022% to 

	0.026% w/w), the average of leaf and stem biomass generally increased 

	in the presence of P-fertilizers due to the changes in the speciation 

	of phosphorus. Leaves of the investigated miscanthus may be of great 

	interest as a catalyst in organic chemistry, since the Ca concentration 

	was up to 9000 mg kg−1 DW. Stems represent a potential biomass that 

	can be used as renewable resource of Lewis acids, currently used 

	in organic syntheses (the sum of Zn, Cu, Mn, Fe, Mg, Si and Al was 

	near 1000 mg kg−1 DW). The percentage of Cd and Pb in leaves and 

	stems of miscanthus did not significantly change with P-fertilizers. 

	Depending on the mesocosm and field experiments, it ranged from 0.004% 

	to 0.016% and from 0.009% and 0.034% for Cd in leaves and stems, 

	respectively, and from 0.004% to 0.015% and from 0.009% and 0.033% 

	for Pb in leaves and stems, respectively.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@book{Pelfrene2020c,

title = {Spécificités des potagers en contexte (péri)urbain au lourd passé industriel},

author = {Aurelie Pelfrêne and Géraldine Bidar and Sébastien Détriché and Christophe Waterlot and Christelle Pruvot and Francis Douay},

year  = {2020},

date = {2020-01-01},

booktitle = {in "Les sols urbains sont-ils cultivables ?" (Editions Quae, Collection Matière à débattres & décider)},

note = {OS},

keywords = {ER4},

pubstate = {published},

tppubtype = {book}

}

@article{ALSOUKI2020110654,

title = {Miscanthus x giganteus culture on soils highly contaminated by metals: Modelling leaf decomposition impact on metal mobility and bioavailability in the soil–plant system},

author = {Al Souki Karim Suhail and Clarisse Liné and Brice Louvel and Christophe Waterlot and Francis Douay and Bertrand Pourrut},

url = {https://www.sciencedirect.com/science/article/pii/S0147651320304930},

doi = {https://doi.org/10.1016/j.ecoenv.2020.110654},

issn = {0147-6513},

year  = {2020},

date = {2020-01-01},

urldate = {2020-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {199},

pages = {110654},

abstract = {Miscanthus x giganteus is suggested as a good candidate for phytostabilization of metal-polluted soils. Its late harvest in winter generates large amounts of leaf litter on the soil surface. However, little is known about the mobility and the bioavailability of metals following leaf decomposition and the consequences on the succeeding culture. Ex situ artificial aging for 1, 3, and 6 months was conducted with miscanthus leaf fragments incorporated into three agricultural soils displaying a gradient concentration in Cd (0.6, 3.1 and 7.9 mg kg−1), Pb (32.0, 194.6 and 468.6 mg kg−1), and Zn (48.4, 276.3 and 490.2 mg kg−1) to simulate the leaf litter input over 20 years of miscanthus culture. We investigated the impacts on physicochemical and biological soil parameters, CaCl2-extractable metal, and their subsequent ryegrass shoot concentrations, and hence on ryegrass health. The results showed that the amended soils possessed higher pH along with greater available phosphorous and soil organic carbon values. The respiratory activity and microbial biomass carbon in the amended soils increased mainly after 1 month of aging, and decreased afterwards. Despite the higher Pb- and Zn–CaCl2 extractability in the amended soils, the phytoavailability slightly increased only in the most contaminated soils. Moreover, leaf incorporation did not affect the ryegrass biomass, photosynthetic pigment contents, nor the antioxidative enzyme activities. Conclusively, leaf incorporation induced slight variations in soil physicochemical and biological parameters, as well as metal extractability, but not to an extent that might cause a considerable threat to the subsequent culture. Nevertheless, these results are preliminary data that require confirmation by long-term in-situ experimentations as they reflect the modelization of long-term impact of leaf decomposition on soil-plant system.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{nokey,

title = {Ecocatalyzed synthesis of valuable therapeutic molecules},

author = {Théo Guérin and Christophe Waterlot and Alina Ghinet},

year  = {2020},

date = {2020-01-19},

urldate = {2020-01-19},

address = {St Anton, Austria},

note = {2nd Alpine Winter Conference on Medicinal and Synthetic Chemistry, St Anton, Austria, COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Dufrenoy2019,

title = {An innovative and efficient method to synthesize meloxicam in one-step procedure with respect to the green chemistry},

author = {Pierrick Dufrénoy and Alina Ghinet and Marie Hechelski and Adam Daïch and Christophe Waterlot},

year  = {2019},

date = {2019-01-01},

journal = {Journal of the Iranian Chemical Society},

volume = {16},

number = {3},

pages = {501-509},

abstract = {An improved procedure for the synthesis of meloxicam drug (methyl 

	4-hydroxy-2-methyl-2H-1,2-benzothiazol-2-amine-3-carboxylate 1,1-dioxide) 

	was described in one-step using mainly impregnated montmorillonite 

	K10 (MK10) with ZnCl2 as a heterogeneous catalyst. This innovative 

	method was compared to the last described procedure employed in the 

	manufacture of this anti-inflammatory drug by means of some metrics 

	used in a first step of the evaluation process of the environmental 

	impact of a chemical transformation. Apart from the yield, which 

	was 90%, atom economy, waste, environmental factor, reaction mass 

	efficiency and stoichiometric factor were calculated as 91.6%, 8.4%, 

	0, 8.1% and 1%, respectively. Interpretation of these metrics was 

	given and highlighted the fact that the strategy used in the current 

	study may be considered as an environmental-friendly and sustainable 

	method that fits well in the green chemistry concepts.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Ghemari2019,

title = {Histopathological changes in the hepatopancreas of Porcellio laevis (Crustacea, Isopoda) after exposure to Cd and Zn mixture},

author = {Chedliya Ghemari and Raja Jelassi and Hajer Khemaissia and Christophe Waterlot and Maryline Raimond and Catherine Souty-Grosset and Francis Douay and Karima Nasri-Ammar},

year  = {2019},

date = {2019-01-01},

booktitle = {2nd Euro-Mediterranean Conference for Environmental Integration, 10-13 October 2019, Sousse (Tunisia)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Ghemari2019a,

title = {Effects of Heavy Metals Artificial Contamination on Porcellio laevis (Latreille, 1804) (Crustacea: Isopoda: Oniscidea)},

author = {Chedliya Ghemari and Christophe Waterlot and Anas Ayari and Francis Douay and Karima Nasri-Ammar},

year  = {2019},

date = {2019-01-01},

journal = {Bulletin of Environmental Contamination and Toxicology},

volume = {103},

pages = {416-420},

abstract = {This study aimed at determining the competition of cadmium (Cd), lead 

	(Pb), zinc (Zn) and copper (Cu) on their assimilation, on the food 

	consumption and the growth of terrestrial isopod Porcellio laevis. 

	Individuals were exposed to artificially contaminated litter of Quercus 

	for 4 weeks and were weekly weighed. At the end of the experiment, 

	the concentration of Cd, Pb, Zn and Cu in individuals were measured 

	by atomic absorption spectrometry. Biological parameters such as 

	growth, and bioaccumulation factor (BAF) were calculated and results 

	from the various treatments were compared. Depending on metals, weight 

	loss or gain were recorded for isopods during the four weeks of exposure. 

	A weight loss was measured on individuals exposed to Cd-contaminated 

	litter whereas a weight gain was highlighted for those exposed to 

	the Zn-contaminated litter. BAF values revealed that P. laevis was 

	macroconcentrator of Zn and Cu and deconcentrator of Cd and Pb},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Hechelski2019a,

title = {The potential of ryegrass (Lolium perenne L.) to clean up multi-contaminated soils from labile and phytoavailable potentially toxic elements to contribute into a circular economy},

author = {Marie Hechelski and Brice Louvel and Pierrick Dufrénoy and Alina Ghinet and Christophe Waterlot},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Science and Pollution Research},

volume = {26},

number = {17},

pages = {17489–17498},

abstract = {Aided phytoremediation was studied for 48 weeks with the aim of reducing 

	extractable and phytoavailable toxic elements and producing potential 

	marketable biomass. In this sense, biomass of ryegrass was produced 

	under greenhouse on two contaminated garden soils that have been 

	amended with two successive additions of phosphates. After the first 

	addition of phosphates, seeds of ryegrass were sown and shoots were 

	harvested twice. A second seedling was performed after carefully 

	mixing the roots from the first production (used as compost), soils 

	and phosphates. Forty-eight weeks after starting the experiments, 

	the concentrations of Cd, Pb, Zn, Cu, Fe, and Mn extracted using 

	the rhizosphere-based method were generally lower than those measured 

	before the addition of phosphates and cultivation (except for Pb 

	and Fe in the most contaminated soil). The concentrations of metals 

	in the shoots of ryegrass from the second production were lower than 

	those from the first (except for Al). The best results were obtained 

	with phosphates and were the most relevant in the lowest contaminated 

	soil, demonstrating that the available metal concentrations have 

	to be taken into account in the management of contaminated soils. 

	In view of the concentration of metals defined as carcinogens, mutagens, 

	and reprotoxics (e.g., Cd, Pb) and those capable to be transformed 

	into Lewis acids (e.g., Zn, Fe), the utilization of ryegrass in the 

	revegetation of contaminated soils and in risk management may be 

	a new production of marketable biomass. The development of phytomanagement 

	in combination with this type of biomass coincided with the view 

	that contaminated soils can still represent a valuable resource that 

	should be used sustainably.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Le-Bot2019,

title = {Simplified method for oral bioaccessibility of metal(loid)s in soils and alternative to the Unified Barge Method},

author = {Barbara Le-Bot and Philippe Glorennec and Christophe Waterlot and Francis Douay and Aurélie Pelfrêne},

year  = {2019},

date = {2019-01-01},

booktitle = {International Societies of Exposure Science (ISES) and Indoor Air Quality and Climate (ISIAQ), 18-22 August 2019, Kaunas (Lithuania)},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pelfrene2019a,

title = {Evaluation of four first tier methods to measure metal(loid) bioaccessibility in polluted soils},

author = {Aurélie Pelfrêne and Barbara Le-Bot and Christophe Waterlot and Philippe Glorennec and Karin Sahmer and Francis Douay},

year  = {2019},

date = {2019-01-01},

booktitle = {SETAC Europe, 29th Annual meeting, 26-30 May 2019, Helsinki (Finland)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Pelfrene2019d,

title = {From environmental data acquisition to assessment of gardeners' exposure: feedback in an urban context highly contaminated with metals},

author = {Aurélie Pelfrêne and Karin Sahmer and Christophe Waterlot and Francis Douay},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Science and Pollution Research},

volume = {26},

number = {20},

pages = {20107-20120},

abstract = {Although growing vegetables in urban gardens has several benefits, 

	some questions in relation with the safety of foods remain when the 

	self-production is carried out on highly contaminated garden soils. 

	To better assess the local population's exposure to Cd and Pb induced 

	by the past activities of a lead smelter, a participatory program 

	was initiated in 115 private kitchen gardens located in northern 

	France to assist gardeners in understanding their soil environment. 

	The challenge included contributing to the database of urban garden 

	soils with the collection of a large number of samples: 1525 crops 

	grouped into 12 types (leaf, fruiting, root, stem and bulbous vegetables, 

	tubers, cabbages, leguminous plants, celeriac, fresh herbs, fruits, 

	and berries), 708 topsoils, and 52 samples of self-produced compost. 

	The main results were as follows: (i) topsoils were strongly contaminated 

	by Cd and Pb compared to regional reference values; (ii) great variability 

	in physicochemical parameters and metal concentrations in topsoils; 

	(iii) the highest concentrations of Cd and Pb for celeriac and fresh 

	herbs and the lowest for fruits and fruiting vegetables; (iv) a high 

	percentage of vegetables that did not comply with the European foodstuff 

	legislation; and (v) most self-produced compost samples were strongly 

	contaminated. This study aimed to raise awareness and generate functional 

	recommendations to reduce human exposure and to provide useful data 

	that could be considered in other environmental contexts.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2019,

title = {Benefits of Ryegrass on Multicontaminated Soils Part 1: Effects of Fertilizers on Bioavailability and Accumulation of Metals},

author = {Christophe Waterlot and Marie Hechelski},

year  = {2019},

date = {2019-01-01},

journal = {Sustainability},

volume = {11},

number = {18},

pages = {5093},

abstract = {Effects of three phosphorus fertilizers on the shoot biomass and on 

	the accumulation of alkali, alkaline earth, and transition metals 

	in the shoots and roots of ryegrass were studied with two contaminated 

	garden soils. Phosphates were added in sustainable quantities in 

	order to reduce the environmental availability of carcinogenic metals 

	(e.g., Cd and Pb) and to enhance the bioavailability of alkali and 

	alkaline earth metals as well as micronutrients needed by plants. 

	Addition of Ca(H2PO4)2 was the most convenient way to (i) limit the 

	concentration of Cd and Pb, (ii) keep constant the transfer of macro- 

	and micronutrient from the soil to the ryegrass shoots, (iii) decrease 

	the availability of metals, and (iv) increase the ratio values between 

	potential Lewis acids and Cd or Pb in order to produce biosourced 

	catalysis. For instance, the real phytoavailability was reduced by 

	27%–57% and 64.2%–94.8% for Cd and Pb, respectively. Interestingly, 

	the real phytoavailability of Zn was the highest in the least contaminated 

	soils. Even if soils were highly contaminated, no visual toxicity 

	symptoms were recorded in the growing ryegrasses. This indicates 

	that ryegrass is suitable for the revegetation of contaminated gardens. 

	To promote the sustainable ryegrass production on contaminated soils 

	for production of new organic fragrance and drugs in green processes 

	according to REACH (Registration, Evaluation, Authorisation, and 

	Restriction of Chemicals) regulation, two processes should be recommended: 

	assisted phytostabilization of the elements, and then assisted phytoextraction 

	by using chelators. View Full-Text},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Hechelski2019b,

title = {Effects of calcium phosphates on the (im)mobilization of metals and nutrients, on the biological activity and on the plant health from multi-contaminated urban soils},

author = {Marie Hechelski and Brice Louvel and Pierrick Dufrénoy and Alina Ghinet and Christophe Waterlot},

year  = {2019},

date = {2019-01-01},

journal = {Water, Air, & Soil Pollution},

volume = {230},

number = {8},

pages = {206-223},

abstract = {Two smelters in the North of France emitted potentially toxic metals 

	for more than a century and today, the resulting contamination represents 

	a risk to human health and affects also the biodiversity. To limit 

	health risks and to improve the soil quality, a study using calcium 

	phosphates (monocalcium phosphate, dicalcium phosphate and a mixture 

	of both salts) and Lolium perenne L was conducted. Through this preliminary 

	investigation, we will try to shed some light about (i) the effects 

	of a sustainable amount of calcium phosphates on the agronomic, biological 

	(microbial and fungi communities) and physiological parameters (chlorophyll 

	a and b, antocyanins, carotenoids) as well as the phytoavailability 

	of potentially toxic metals and nutrients in time, and (ii) the potential 

	use of contaminated biomass from ryegrass as a source of new valorisation 

	ways instead of using it as contaminated compost by gardeners. Although 

	slight variations in pH and significant increases of assimilable 

	phosphorus after adding calcium phosphates were registered, the physiology 

	of plants and the biological parameters were statistically unchanged. 

	The germination of the ryegrass seeds was favoured with calcium phosphates 

	regardless the contamination level of the studied soils. No clear 

	effects of calcium phosphates on the microbial and fungi communities 

	were detected. In contrast, results indicated relationships between 

	the physicochemical parameters of soils, their contamination level 

	and the composition of fungal communities. Indeed, for one of the 

	soils studied, calcium could limit the transport of nutrients, causing 

	an increase in fungi to promote again the transfer of nutrients. 

	Surprisingly, the phytoavailability of Pb increased in the most contaminated 

	soil after adding dicalcium phosphate and the mixture of phosphates 

	whereas a slight decrease was highlighted for Cd and Mn. Although 

	minor changes in the phytoavailability of potentially toxic metals 

	were obtained using calcium phosphates, the ability of ryegrass to 

	accumulate Zn and Ca (up to 600 and 20,000 mg kg−1, respectively) 

	make possible to qualify this plant as a bio ‘ore’ resource.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Hechelski2019ab,

title = {A sustainable approach to manage metal-contaminated soils: a preliminary greenhouse study for the possible production of metal-enriched ryegrass biomass for biosourced catalysts},

author = {Marie Hechelski and Brice Louvel and Pierrick Dufrénoy and Alina Ghinet and Christophe Waterlot},

year  = {2019},

date = {2019-01-01},

journal = {Environmental Monitoring and Assessment},

volume = {191},

number = {10},

pages = {626-640},

abstract = {Two kitchen garden soils (A and B) sampled in contaminated areas were 

	amended using phosphates in sustainable quantities in order to reduce 

	the environmental availability of potentially toxic inorganic elements 

	(PTEs) and to favour the availability of alkali, alkali earth and 

	micronutrients. The environmental availability of PTEs was evaluated 

	using a potential plant for revegetation of contaminated soils (ryegrass) 

	and a mixture of low molecular weight organic acids. Despite the 

	highest contamination level of B, the concentration of metals was 

	highest in the ryegrass shoots grown on A for the two harvests. These 

	results correlated well with those obtained using low molecular weight 

	organic acids for Cd, Zn and Cu, whereas this mixture failed to represent 

	the transfer of nutrients due to the presence of biological and physiological 

	mechanisms. The statistical differences between the biomass of ryegrass 

	obtained at the first and the second harvests were attributed to 

	the decrease of available potassium, implicated in the growth and 

	development of plants. Phosphates increased the ratios Zn/Cd, Zn/Pb 

	and Zn/Cu up to 176 ± 48, 38 ± 6 and 80 ± 12, respectively, and made 

	possible the reduction of the concentration of Cd and Pb in the shoots 

	of ryegrass by 22% and 25%, respectively. The concentration of Zn 

	in the shoots of ryegrass from the first and the second harvests 

	grown on soil A were in the range 1050–2000 mg kg−1, making this 

	plant a potential biomass to (i) produce biosourced catalysts for 

	organic chemistry applications in a circular economy concept and 

	(ii) limit human exposure to commercial Lewis acids. A preliminary 

	application was identified.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Pelfrene2019f,

title = {Une méthode simplifiée pour estimer la bioaccessibilité des polluants métalliques dans les sols},

author = {Aurélie Pelfrêne and Barbara Le-Bot and Christophe Waterlot and Philippe Glorennec and Karin Sahmer and Francis Douay},

year  = {2019},

date = {2019-01-01},

booktitle = {4ème Rencontres nationales de la Recherche sur les sites et sols pollués, Le Beffroi de Montrouge, 26-27 novembre 2019, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Waterlot2019b,

title = {Benefits of Ryegrass on Multicontaminated Soils Part 2: Green process to provide Idrocilamide},

author = {Christophe Waterlot and Pierrick Dufrénoy and Marie Hechelski and Brice Louvel and Adam Daïch and Alina Ghinet},

year  = {2019},

date = {2019-01-01},

journal = {Sustainability},

volume = {11},

number = {23},

pages = {6685-6694},

abstract = {A restoration of highly contaminated garden soil is proposed as a 

	greener alternative to the production of vegetables. Depending on 

	potentially toxic elements and their concentration, ryegrass shoots 

	accumulate these elements in sufficient quantity to be used as a 

	catalyst in organic synthesis. The analysis of Cd, Pb, Zn, Cu, Fe, 

	Mn, and Al in ashes issued from the calcination of ryegrass shoots 

	revealed that the concentration of Zn was highest (>7000 mg kg–1). 

	The ratios between potential Lewis acids (Zn, Cu, Fe, Mn, and Al) 

	to carcinogenic metals (Cd or Pb) were 191 ± 7 for LA/Cd and 235 

	± 13 for LA/Pb, making the shoots of ryegrass suitable for the production 

	of Zn-rich polymetallic biosourced catalysts. This material was used 

	in the synthesis of idrocilamide under free-solvent condition, providing 

	the drug in a good yield (69.9%). Data show that a limitation of 

	waste, a maximization of the material incorporation in the process, 

	a minimization of the steps, and an optimization of the stoichiometric 

	factor are the main innovative factors in the current process in 

	comparison with those previously reported.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Leclercq-Dransar2019,

title = {Distribution of metals and cell wall compounds in leaf parts of three tree species suitable for the phytomanagement of heavy metal–contaminated soils},

author = {Julie Leclercq-Dransart and Sylvain Demuynck and Christophe Waterlot and Géraldine Bidar and Karin Sahmer and Céline Pernin and A Deram and Alain Leprêtre and Francis Douay},

year  = {2019},

date = {2019-01-01},

journal = {Water Air and Soil Pollution},

volume = {230},

number = {237},

pages = {1-16},

abstract = {Phytomanagement used on soils contaminated with metals aims to stabilize 

	them in the soil. It generally uses less demanding tree species such 

	as maple, poplar, and black locust. However, it is important to consider 

	the rate of accumulation of metals in the leaves as well as their 

	location (leaf blade/petiole) together with the contents of cell 

	wall compounds (hemicelluloses, cellulose, lignin). These two aspects 

	are likely not only to have repercussions on the decomposition of 

	litter but also to lead to a possible transfer ofmetal contamination 

	into the food chain via soil decomposers/detritivorous. A successful 

	phytoremediation is therefore the result of compromise: no tree species 

	can meet all these criteria. The results obtained show that poplar 

	is not recommended due to a high accumulation of Cd and Zn. The most 

	appropriate species among our 3 studied species, which seem the most 

	suitable in terms of remediation of metal polluted soils, could be 

	the black locust and the maple which are less susceptible to contribute 

	to the transfer of exogenous metals such as Cd to the trophic chain. 

	However, maple strongly accumulates Pb in the leaf blade and it is 

	susceptible to degrade quickly due to its high hemicelluloses and 

	cellulose contents and low lignin content. The black locust accumulates 

	metals mainly in the petiole (little consumed by detritivorous). 

	However, its exotic character and high lignin content may limit the 

	interest of its use.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2018,

title = {Alternative approach to the standard, measurements and testing programme used to establish phosphorus fractionation in soils},

author = {Christophe Waterlot},

year  = {2018},

date = {2018-01-01},

journal = {Analytica Chimica Acta},

volume = {1003},

pages = {26-33},

abstract = {The fractionation of phosphorus in 9 soils was established according	to the standards, measurements and testing (SMT) programme. Five fractions were so defined and phosphorus was analysed by spectrophotometry. In parallel, the phosphorus extracted in each fraction was determined using graphite furnace atomic absorption spectrometry (GFAAS) after	validation of this technique using certified reference materials. Phosphorus in soils was named total phosphorus, inorganic and organic phosphorus, apatite and non-apatite inorganic phosphorus, depending on extractants. The use of both analytical techniques revealed differences between the concentration of phosphorus in soluble extracts and highlighted the fact that spectrophotometry was a selective analytical technique. In view of the resourceand time-consuming of the SMT procedure and the results obtained in the present study, an alternative method was proposed to estimate the fractionation of phosphorus in soil in order to precise the potential effects of phosphorus on plant nutrition when plant biomass is produced as part of metal-contaminated soil management. (C) 2017 Elsevier B.V. All rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Waterlot2018a,

title = {From the contaminated soils to the ecocatalysts: An original investigation},

author = {Christophe Waterlot and Marie Hechelski},

year  = {2018},

date = {2018-01-01},

booktitle = {INTECHEM PROCESS, 7-8 mars 2018, Compiègne},

abstract = {The number of contaminated sites by metals and metalloids (As, Cd,	Cu, Pb and Zn) was around 1 250,000 in Europe in 2016. Most of them are located in France, notably in Northern France where numerous habitants live due to the high past industrial activities (mining, metallurgical, inorganic and organic chemistry). Consequently, environmental and sanitary problems like degradation and perturbation of soil activities (loss of the biodiversity…), non-compliant agricultural commodity,	production of contaminated vegetables, lead poisoning and different	types of symptoms in relation with metal human exposure were highlighted. This explains why the management of these contaminated soils (agricultural, urban and forest) is a great concern in the Hauts-de-France region	and particularly in the contaminated area affected by the dust emission of two former lead and zinc smelters.Physical and chemical techniques (soil removal, soil washing, electrokinetic…) are widely used to remediate contaminated soils. However, most of them are expensive and destructive (fauna and flora are highly impacted and the resulting soils are often non-productive).To avoid the dispersion of contaminated soil particles, to restore contaminated sites and ecosystems and to maintain an economic activity in this area, an environmental friendly management based on the concept of assisted-phytoremediation and 

assisted-phytoextraction was studied. From this approach, the first interest was to immobilise the carcinogenic metals (Cd, Pb) and the second was to increase non-carcinogenic metal uptake by plants in order to elaborate new heterogeneous catalysts (called ecocatalysts) from the plant biomass. These bio-sourced catalysts were used in organic synthesis to produce pharmaceuticals taking into account the green chemistry concept. It was highlighted that these new catalysts were reusable, increased the reaction yields while minimizing the production of waste.},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Dufrenoy2018,

title = {Ecocatalysis for the synthesis of high-added molecules for therapeutic purposes. Innovative techniques for chemistry and processes},

author = {Pierrick Dufrénoy and Marie Hechelski and Christophe Waterlot and Alina Ghinet},

year  = {2018},

date = {2018-01-01},

booktitle = {INTECHEM PROCESS, 7-8 mars 2018, Compiègne},

note = {INV},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Ghemari2018,

title = {Measure of environmental stress on Porcellio laevis Latreille, 1804 sampled near active Tunisian industrial areas},

author = {Chedliya Ghemari and Anas Ayari and Nabil Hamdi and Christophe Waterlot and Francis Douay and Karima Nasri-Ammar},

year  = {2018},

date = {2018-01-01},

journal = {Ecotoxicology},

volume = {27},

number = {6},

pages = {729-741},

abstract = {This study aimed to observe the type of asymmetry exhibited by Porcellio 

	laevis sampled from 15 sites belonging to Tunisian industrialized 

	areas. Physicochemical parameters such as pH, organic matter and 

	CaCO3 contents were measured in soils. Moreover, Cd, Pb, Zn, and 

	Cu concentrations were determined in both soils and woodlice. Additionally, 

	10 metrical traits were measured to evaluate the type of asymmetry 

	on individuals: the basis, the second and the third articles of the 

	antenna, the first article of the flagellum of the antenna and the 

	merus, the carpus, and the propodus of the sixth and the seventh 

	pereopods. Among the 531 measured individuals, 432 exhibited fluctuating 

	asymmetry (FA) while the remaining individuals exhibited antisymmetry 

	or directional asymmetry. The data obtained were analyzed using a 

	multivariate statistical analysis. Contrary to our hypothesis, the 

	results showed that individuals from contaminated sites have a low 

	FA level, whereas those from uncontaminated sites have a high FA 

	level, particularly females but with some exceptions. Variations 

	in FA level in the traits and populations studied and its usefulness 

	as a stress indicator were discussed.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Hechelski2018,

title = {An original approach in green chemistry: From assisted-phytoremediation of contaminated soil to upcycling of plant biomass for biosourced catalyst production},

author = {Marie Hechelski and Pierrick Dufrénoy and Brice Louvel and Alina Ghinet and Christophe Waterlot},

year  = {2018},

date = {2018-01-01},

booktitle = {International Conference Contaminated Sites 2018, 8-10 October 2018, Banská Bystrica (Slovak Republic)},

abstract = {A kitchen garden soil was sampled in a contaminated urban area located 

	in the north of France. Samples were air-dried and crushed to pass 

	through a 10-mm stainless steel sieve. Due to the high heterogeneity 

	of garden soils in the studied area, much attention have been paid 

	on the homogeneity of soil samples. After this step, the mass of 

	sampled soils (48 kg) was divided to obtain four subsamples Each 

	of them was divided in six replicates, unamended or amended using 

	dicalcium phosphate (DCP) or monocalcium phosphate (MCP) or a mixture of these two compounds (MxP=75%DCP+25%MCP) The phosphorous amendments 

	were added into the soil in small quantity (0.02 %) with the aim 

	at reducing the environmental availability of carcinogenic metals 

	and to increase the Zn availability (Figure 1) After the stabilisation 

	period (2 months) in a greenhouse, 1 5 g of ryegrass seeds (Lolium 

	perenne L.) were sown in the 24 containers. Eight weeks after sowing, 

	ryegrass shoots were harvested, oven-dried at 40 °C and calcined 

	at 500 °C in a muffle furnace (Nabertherm P330, Lilienthal, Germany).},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Hechelski2018a,

title = {From Conventional Lewis Acids to Heterogeneous Montmorillonite K10: Eco-Friendly Plant-Based Catalysts Used as Green Lewis Acids},

author = {Marie Hechelski and Alina Ghinet and Brice Louvel and Pierrick Dufrénoy and Benoît Rigo and Adam Daïch and Christophe Waterlot},

year  = {2018},

date = {2018-01-01},

journal = {ChemSusChem},

volume = {11},

number = {8},

pages = {1249-1277},

abstract = {The concept of green chemistry began in the USA in the 1990s. Since 

	the publication of the 12 principles of this concept, many reactions 

	in organic chemistry have been developed, and chemical products have 

	been synthesized under environmentally friendly conditions. Lewis 

	acid mediated synthetic transformations are by far the most numerous 

	and best studied. However, the use of certain Lewis acids may cause 

	risks to environmental and human health. This Review discusses the 

	evolution of Lewis acid catalyzed reactions from a homogeneous liquid 

	phase to the solid phase to yield the expected organic molecules 

	under green, safe conditions. In particular, recent developments 

	and applications of biosourced catalysts from plants are highlighted.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Hechelski2018b,

title = {Phytomanagement of contaminated soils by potentially toxic metals in urban areas: A new approach combining sustainable amount of phosphates and ryegrass},

author = {Marie Hechelski and Brice Louvel and Pierrick Dufrénoy and Alina Ghinet and Christophe Waterlot},

year  = {2018},

date = {2018-01-01},

booktitle = {26e Réunion des Sciences de la Terre, 22-26 octobre 2018, Lille},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Sahmer2018,

title = {Modélisation de l’accumulation du cadmium dans des légumes en fonction des concentrations extractibles des sols},

author = {Karin Sahmer and A Potel and Christophe Waterlot and Aurélie Pelfrêne},

year  = {2018},

date = {2018-01-01},

booktitle = {Chimiométrie XIX, 30-31 janvier 2018, Paris},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Waterlot2018c,

title = {Effets des teneurs en carbonates sur la distribution des éléments métalliques de sols contaminés évaluée au moyen de la procédure validée par le Standards, Measurements and Testing Programme},

author = {Christophe Waterlot},

year  = {2018},

date = {2018-01-01},

journal = {Spectra Analyse},

volume = {321},

pages = {54-60},

note = {ACLN},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2018ab,

title = {Core-shell Particles: A Way to Greening Liquid Chromatography in Environmental Applications},

author = {Christophe Waterlot and Alina Ghinet and Emmanuelle Lipka},

year  = {2018},

date = {2018-01-01},

journal = {Current Chromatography},

volume = {5},

number = {2},

pages = {78-90},

abstract = {Background: Since the 1990s, technical developments and theoretical 

	studies of columns packed with core-shell and sub-core-shell particles 

	demonstrated improved efficiency separation, time reduction, solvent 

	use than fully porous columns. Thanks to this chromatographic system, 

	environmental pollutants are being analysed under green conditions. 

 

	Objective: This paper presents theoretical aspects and a review of 

	the main features of core-shell technology focusing essentially on 

	environmental applications. 

 

	Method: The main advantages of core-shell columns may be summarized 

	as follows: i) reduction of the eddy dispersion A term; ii) homogeneity 

	of the particle size distribution reducing the velocity bias; (iii) 

	superior mass transfer kinetics; iv) better performance of the separation 

	(faster and greater). 

 

	Results: Systematic comparisons with fully porous particles undeniably 

	highlighted the benefits of core-shell technology in the separation 

	of a great number of pollutants and “micropollutants” of different 

	molecular weights which surround us and have an impact on the ecosystem. 

 

	Conclusion: Core-shell particles are emerging as green substrates 

	for the analysis of chemical species problematic for environment. 

	The environmental gain in terms of limitation of analysis time and 

	solvent consumption has been demonstrated. The current manuscript 

	proposes an update of the literature devoted to the use of core-shell 

	particles in environmental applications. Future trends in the field 

	and the expectations of the scientific community are also described 

	since increasing attention is being paid to the detection of environmental 

	pollutants.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Janus2018b,

title = {Do biochars influence the availability and human oral bioaccessibility of Cd, Pb, and Zn in a contaminated slightly alkaline soil?},

author = {Adeline Janus and Christophe Waterlot and Sophie Heymans and Christophe Deboffe and Francis Douay and Aurélie Pelfrêne},

year  = {2018},

date = {2018-01-01},

journal = {Environmental Monitoring and Assessment},

volume = {190},

number = {218},

pages = {1-13},

abstract = {Different remediation techniques have been used to restore metal-contaminated 

	sites, including stabilizing metals by adding amendments to the soils. 

	This study experimented three biochars, made from wood and miscanthus, 

	cultivated on contaminated and uncontaminated soils, used as amendments 

	at a 2% application rate on a metal-contaminated soil for 9 months 

	in laboratory-controlled conditions. The objective was to evaluate 

	whether biochars were able to decrease the availability and human 

	oral bioaccessibility of metals in an alkaline soil. To meet this 

	goal, the modifications of the soil’s physicochemical parameters, 

	metal distribution in soil, and human bioaccessibility were evaluated 

	at different sampling times. The results showed that biochar application 

	to the alkaline soil did not always decrease the soil metal availability, 

	which challenges the value of using biochars in already slightly 

	alkaline soils at a low application rate. However, differences in 

	efficiency between the three biochars tested were highlighted. The 

	biochar produced with miscanthus cultivated on uncontaminated soil 

	led to higher soil metal bioaccessibility. Moreover, because of the 

	absence of any increase in soil metal availability with the biochar 

	produced from biomass cultivated on contaminated soil, the use of 

	such biochars can be recommended for the remediation of contaminated 

	soil.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Adusei-Gyamfi2017,

title = {Elimination of metallic pollutants from contaminated aqueous solution using different iron particles},

author = {Junias Adusei-Gyamfi and Alexandre Berquand and Michael Molinari and Jacques Cohen and Christophe Waterlot and Victor Acha},

year  = {2017},

date = {2017-01-01},

booktitle = {Journées Condorcet 2017, Institut Universitaire de Technologie A, Université de Lille, 8-9 juin 2017, Lille},

abstract = {Three iron based reactants; nano zero valent iron, magnetite and micro iron particles were studied to access their efficiencies in remediating metallic polluted aqueous media. Iron particles of such size (nanoscale) were chosen because they can be 10 to 1,000 times highly reactive	than the granular particles and their sorption capacity is also much higher. TEM analysis of the synthesized magnetite showed an average particle size of 5 nm and with a generally pretty rounded shape. The pollutants studied were Cd, Pb, Ni and Cu. Two approaches were used; univariate analysis and multivariate analysis using CORICO design. Comparing the three different iron particles studied under	four univariate conditions, the removal efficiency of the three iron products can be ranked in the order; magnetite > nano powder > micro particles. For the multivariate analysis, it was observed that the most important condition for remediating metals when using magnetite is the concentration of the magnetite followed by contact time, pH and pollutant concentration, respectively.},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Dufrenoy2017,

title = {Valorization of montmorillonite K10-supported Lewis acids as new catalysts for the synthesis of new antitumor compounds},

author = {Pierrick Dufrénoy and Benoit Rigo and Adam Daïch and Marie Hechelski and Christophe Waterlot and Alina Ghinet},

year  = {2017},

date = {2017-01-01},

booktitle = {4th French-Romanian Colloquium on Medicinal Chemistry, 5-7 October 2017, Iasi (Roumania)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Dufrenoy2017a,

title = {Valorization of new eco-catalysts in the synthesis of new antitumor compounds},

author = {Pierrick Dufrénoy and Benoit Rigo and Adam Daïch and Christophe Waterlot and Marie Hechelski and Alina Ghinet},

year  = {2017},

date = {2017-01-01},

booktitle = {Journées Nord-Ouest Européennes des Jeunes Chercheurs et du Groupe Français des Polymères Grand Ouest-JNOEJC/GFP. 08-09 juin 2017, Caen},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Dufrenoy2017b,

title = {Valorization of supported catalysts in the synthesis of new farnesyltransferase inhibitors},

author = {Pierrick Dufrénoy and Benoit Rigo and Adam Daïch and Christophe Waterlot and Marie Hechelski and Alina Ghinet},

year  = {2017},

date = {2017-01-01},

booktitle = {Journées du Drug Discovery, Faculté de pharmacie, Lille 2, 8 décembre 2017, Lille},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Dufrenoy2017c,

title = {Valorization of new eco-catalysts in the synthesis of anti-inflammatory compounds},

author = {Pierrick Dufrénoy and Benoit Rigo and Christophe Waterlot and Marie Hechelski and Adam Daïch and Alina Ghinet},

year  = {2017},

date = {2017-01-01},

booktitle = {Journées des Jeunes Chercheurs de la Société de Chimie Thérapeutique, 08-10 février 2017, Faculté de pharmacie Châtenay-Malabry},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Hechelski2017,

title = {Toward a new approach in drug design: from polluted soil an non hyper-accumulating plants to anti-inflammatory agent},

author = {Marie Hechelski and Pierrick Dufrénoy and Brice Louvel and Alina Ghinet and Christophe Waterlot},

year  = {2017},

date = {2017-01-01},

booktitle = {4th French-Romanian Colloquium on Medicinal Chemistry, 5-7 October 2017, Iasi (Roumanie)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Janus2017a,

title = {Determination of PAHs by ultra fast liquid chromatography using a coreshell technology – Application to their determination after using biochar as adsorbent},

author = {Adeline Janus and Anaïs Goulas and Aurélie Pelfrêne and Francis Douay and Christophe Waterlot},

year  = {2017},

date = {2017-01-01},

journal = {Measurement},

volume = {106},

pages = {137-142},

abstract = {The c18 column (250 mm 4.6 mm) packed with 5 lm core-shell particles was used in an effort to optimize the separation of 8 polycyclic aromatic hydrocarbons under very low pressure values (44–52 bars), low temperature (16 c) and in 13 min. chromatogram and peaks of each polycyclic aromatic hydrocarbon studied, obtained using an ultra fast liquid chromatography fitted with this column and equipped with a diode array detector, were compared with those obtained using two other traditional c18 columns, packed with fully porous 5 lm particles, usually used in our laboratory and specifically designed for the separation of polycyclic aromatic hydrocarbons. thinnest peaks, highest sensitivity and efficiency are the main results related to the core-shell technology of the recent kinetex column compared to more common c18 columns due to the bed uniformity and packing quality of the recent core-shell column. on the other hand, reduced retention time was highlighted for each polycyclic aromatic hydrocarbon using the column packed with core-shell particles resulting from the lower surface area of these particles compared to the totally porous particles of the both other columns used. limits of detection and quantification, linearity range and repeatability were determined for the method developed with the core-shell column. a practical application was realized by evaluating the sorption capacity of the 8 polycyclic aromatic hydrocarbons studied in mixture using a biochar made from woody plants. to this end, the biochar was mixed with aqueous solution spiked with the mixture of polycyclic aromatic hydrocarbons and a kinetic study of their sorption was conducted. sorption percentage was higher than 89% for each polycyclic aromatic hydrocarbon studied showing that the studied biochar could be good and low cost alternative products for the sorption of this type of pollutants.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Janus2017b,

title = {Value of biochars from Miscanthus x giganteus cultivated on contaminated soils to decrease the availability of metals in multicontaminated aqueous solutions},

author = {Adeline Janus and Aurélie Pelfrêne and Karin Sahmer and Sophie Heymans and Christophe Deboffe and Francis Douay and Christophe Waterlot},

year  = {2017},

date = {2017-01-01},

journal = {Environmental Science and Pollution Research},

volume = {24},

number = {22},

pages = {18204-18217},

abstract = {The objective of this study was to evaluate the sorption efficiency of eight biochars, made from miscanthus x giganteus cultivated on contaminated agricultural soil, in aqueous solutions contaminated with metals alone or mixed with polycyclic aromatic hydrocarbons. these biochars were produced in different pyrolysis conditions (temperature, 400/600 °c; heating rate, 5/10 °c min−1; duration, 45/90 min) and compared with an uncontaminated commercialized biochar made of wood. the physicochemical characterization of the miscanthus biochars confirmed the impact of the pyrolysis on the biochar parameters with substantial differences between the biochars in terms of ph, cation exchange capacity, and specific surface area. the sorption experiment showed higher sorption efficiency of cd, pb, and zn for the miscanthus biochars produced at 600 °c compared with the biochars produced at 400 °c when the aqueous solutions were mono- or multicontaminated. furthermore, the desorption study showed that the sorption process was largely irreversible. therefore, the high sorption capacity of miscanthus biochars and the low sorption reversibility confirmed that these biochars are a suitable sorbent for metals.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Pelfrene2017c,

title = {A decision-making tool for managing franch polluted sites: Toward an alternative approach to the Unified Barge Method},

author = {Aurélie Pelfrêne and Christophe Waterlot and Barbara Le-Bot and Philippe Glorennec and Francis Douay},

year  = {2017},

date = {2017-01-01},

booktitle = {6-9 November 2017, Varsovie (Pologne)},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Waterlot2017b,

title = {Green revolution in chemistry: existing and future challenges},

author = {Christophe Waterlot},

year  = {2017},

date = {2017-01-01},

booktitle = {4th French-Romanian Colloquium on Medicinal Chemistry, 5-7 October 2017, Iasi (Roumanie)},

note = {INV},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Waterlot2017b,

title = {Chemical availability of Cd, Pb and Zn in anthropogenically polluted soil: Assessing the geochemical reactivity and oral bioaccessibility},

author = {Christophe Waterlot and Francis Douay and Aurélie Pelfrêne},

year  = {2017},

date = {2017-01-01},

journal = {Pedosphere},

volume = {27},

number = {3},

pages = {616-629},

abstract = {The most recent in vitro tests used to determine metal bioaccessiblility are generally time-consuming and expensive. this study aimed at determining potential relationships between the concentrations of metals extracted using single-extraction methods and the concentrations of bioaccessible metals assessed by a harmonised in vitro test, the unified barge method (ubm). a total number of 27 soil samples were collected from kitchen gardens and lawns with various physicochemical parameters and contamination levels. significant relationships were obtained between cd, pb and zn extracted in gastric and gastrointestinal phases and using single extractions. the best relationhips were established using acetic and citric acids for cd, whereas for pb, citric acid and ethylenediaminetetraacetic acid (edta) were identified as the best extractants. these relationships were improved by means of a linear multiple regression with a downward stepwise procedure involving agronomic parameters (soil cation exchange capacity and assimilated p). this method highlighted the fact that the cation exchange capacity and p contents in soils were the two main parameters that controlled the human bioaccessibility of cd, pb and zn in the gastric phase. besides, the metal concentrations extracted with the acetic and citric acids correlated well with the metal concentrations in the gastric and gastrointestinal phases, suggesting that the bioaccessible metals were mainly in a soluble form, weakly bound to the organic matter and associated with the carbonates and the fe and mn oxides/hydroxides in soils.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2017a,

title = {Impact of a phosphate amendment on the environmental availability and phytoavailability of Cd and Pb in moderately and highly carbonated kitchen garden soils},

author = {Christophe Waterlot and Christelle Pruvot and Franck Marot and Francis Douay},

year  = {2017},

date = {2017-01-01},

journal = {Pedosphere},

volume = {27},

number = {3},

pages = {588-605},

abstract = {The behaviour of metals mainly depends on soil ph, carbonate contents and contamination level, which should be considered for the management of contaminated soils. in this study, kitchen garden topsoils (0–25 cm) were sampled from the area around three smelters in france, with different cd and pb contamination levels. effect of a phosphate amendment (a mixture of diammonium phosphate and hydroxyapatite) on the environmental availability and phytoavailability of cd and pb was evaluated by different chemical extractions and cultivating lettuce (lactuca sativa l.), respectively. changes in the distribution of cd and pb were found in most contaminated soils after phosphate amendment. an increase of cd and pb in the residual phase was highlighted in almost all carbonated contaminated soils, whereas a decrease of pb in the exchangeable, water and acid- soluble phase was observed in most contaminated soils with the lowest carbonate contents. the concentrations of extractable cd and pb using calcium chloride and acetic and citric acids generally decreased after the soil amendment. lettuces grown on amended soils were acceptable for human consumption as regard to pb concentration. in contrast, some lettuces were unacceptable for human consumption, since the concentrations of cd in the leaves were higher than the european legislation limit. surprisingly, in carbonated soils with very low concentration of cd, the cd concentrations in lettuce reached up to the european legislation limit, making the lettuce unacceptable for human consumption. our study highlighted the fact that the total metal concentration in soils does not always allow to predict the metal accumulation in the edible parts of vegetables in order to make a judgement about their acceptability or unacceptability for human consumption. key words: chemical amendment, contamination, immobilisation, lettuce, metal, urban soil},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Ghemari2017,

title = {Assessment of heavy metals in soil and terrestrial isopod Porcellio laevis in Tunisian industrialized areas},

author = {Chedliya Ghemari and Christophe Waterlot and Anas Ayari and Julie Leclercq-Dransart and Francis Douay and Karima Nasri-Ammar},

year  = {2017},

date = {2017-01-01},

journal = {Environmental Earth Sciences},

volume = {76},

pages = {623-636},

abstract = {In this study, data on several metals (cd, pb, zn and cu) in soil and isopod porcellio laevis taken at 21 sites from the most important industrial areas in tunisia (bizerte, nabeul, zaghouan, sfax and gabes) were presented. heavy metal concentrations in both soil samples and isopods were determined using atomic absorption spectrometry. soil contamination was estimated using the contamination factor (cf). on the other hand, the bioaccumulation factor (baf) was determined to estimate metal accumulation in isopods. the cf values show that the level of contamination varies between sampled soils, which may be due to the source of pollution at each site. the baf values allow defining the order of accumulation in p. laevis which was classified for the majority of the sampled sites as a macro-concentrator of cu and zn and a deconcentrator of cd with some exceptions. a principal component analysis (pca) was conducted between soil properties (ph, om and caco3) and metal concentrations in soils. through pca, we obtained four groups in which soils were distinguished by their physicochemical properties and their metal concentrations. moreover, linear multiple regressions with a downward stepwise procedure were conducted to test the relationships between the physicochemical parameters and metal concentrations in both soils and isopods. thus, positive correlations (0.78 < r 2 < 0.99) were obtained for pb considering dataset from the groups 1, 2 and for zn with data of groups 2 and 3. finally, results showed that p. laevis could be used as a bio-indicator for monitoring and reducing the impact of pollution in terrestrial ecosystems},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Bidar2016,

title = {Sustainability of an in situ aided phytostabilisation on highly contaminated soils using fly ashes: Effects on the vertical distribution of physicochemical parameters and trace elements},

author = {Géraldine Bidar and Christophe Waterlot and Anthony Verdin and Nicolas Proix and Dominique Courcot and Sébastien Détriché and Hervé Fourrier and Antoine Richard and Francis Douay},

year  = {2016},

date = {2016-01-01},

journal = {Journal of Environmental Management},

volume = {171},

pages = {204-216},

abstract = {Aided phytostabilisation using trees and fly ashes is a promising technique which has shown its effectiveness in the management of highly metal-contaminated soils. however, this success is generally established based on topsoil physicochemical analysis and short-term experiments. this paper focuses on the long-term effects of the afforestation and twofly ashes (silico-aluminous and sulfo-calcic called fa1 and fa2, respectively) by assessing the integrity of fly ashes 10 years after their incorporation into the soil as well as the vertical distribution of the physicochemical parameters and trace elements (tes) in the amended soils (f1 and f2) in comparison with a non-amended soil (r). ten years after the soil treatment, the particle size distribution analysis between fly ashes and their corresponding masses (fly ash þ soil particles) showed a loss or an agglomeration of finer particles. this evolution matches with the appearance of gypsum (caso4 2h2o) in fa2m instead of anhydrite (caso4), which is the major compound of fa2. this finding corresponds well with the dissolution and the lixiviation of ca, s and p included in fa2 along the f2 soil profile, generating an accumulation of these elements at 30 cm depth. however, no variation of te contaminationwas found between 0 and 25 cm depth in f2 soil except for cd. conversely, cd, pb, zn and hg enrichmentwas observed at 25 cm depth in the f1 soil, whereas no enrichmentwas observed for as. the fly ashes studied, and notably fa2, were able to reduce cd, pb and zn availability in soil and this capacity persists over the time despite their structural and chemical changes.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Janus2016,

title = {Value of Miscanthus biochars to decrease the availability of metals in aqueous solutions},

author = {Adeline Janus and Aurélie Pelfrêne and Karin Sahmer and Sophie Heymans and Christophe Deboffe and Francis Douay and Christophe Waterlot},

year  = {2016},

date = {2016-01-01},

booktitle = {International Conference Contaminated Sites 2016, 12-13 September 2016, Bratislava (Slovakia)},

abstract = {Introduction in europe, more than 2.5 millions of potentially contaminated site a new approach: use biochars produced with biomass cultivated on agricultural soils contaminated by metals biochar production materials and methods results conclusions the pyrolysis process impacts the biochar characteristics. the biochars produced at 600 °c show the highest ssa and ph and the lowest cec. the biochars show a high efficiency to sorb the three metals, especially those produced at 600°c. the presence of metals and pahs affect the removal efficiency, notably for the biochars produced at 400°c. the high sorption capacity of miscanthus biochars confirmed these biochars as suitable sorbent for cd, pb and zn.},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Nsanganwimana2016,

title = {Metal, nutrient and biomass accumulation during the growing cycle of Miscanthus established on metal-contaminated soils},

author = {Florien Nsanganwimana and Christophe Waterlot and Brice Louvel and Bertrand Pourrut and Francis Douay},

year  = {2016},

date = {2016-01-01},

journal = {Journal of Plant Nutrition and Soil Science},

volume = {179},

number = {2},

pages = {257-269},

abstract = {The energy crop miscanthus presents high potentials for phytomanagement. its shoot yield and nutrient accumulation has been extensively characterized in uncontaminated agricultural soils, while very little is known for metal-contaminated conditions. this study aimed at assessing potential differences in dry matter and metal and nutrient accumulation of the standing aerial biomass in miscanthus (m. x giganteus) growing in situ on agricultural plots presenting different soil cd, pb, and zn concentrations. plant samplings were conducted monthly along the growing period from may to december. cadmium, pb, zn, and the concentrations of the nutrients n, p, k, ca, mg, and na were determined in leaves and stems separately. during the growing phase, the maximum dry matter was reached in early in autumn. whatever the organ, cd and zn concentrations were higher on contaminated than on uncontaminated plots. during summer and autumn, zn and pb concentrations were higher in leaves than in stems whereas cd concentrations did not significantly differ between the organs. concentrations of n, p, k, and mg decreased across the study period whereas those of ca and na increased. overall, metal and nutrient concentrations depended on plant organ and its development stage. the dry matter and nutrient accumulation patterns were not different between contaminated and uncontaminated plots. the significance of these findings is discussed in light of best phytomanagement practices and potential uses of miscanthus biomass.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2016,

title = {Temperature effects on retention and separation of PAHs in reversed-phase liquid chromatography using columns packed with fully porous and core-shell particles},

author = {Christophe Waterlot and Anaïs Goulas},

year  = {2016},

date = {2016-01-01},

journal = {Journal of Chemistry},

volume = {2016},

pages = {1-12},

abstract = {Effects of temperature on the reversed-phase chromatographic behavior of pahs were investigated on three columns. the first was the recent c18 column (250 mm × 4.6 mm) packed with 5 µm core-shell particles while the others were more conventional c18 columns (250 mm × 4.6 mm) packed with fully porous particles. among the 16 pahs studied, special attention has been paid to two pairs of pahs, fluorene/acenaphthene and chrysene/benzo[a]anthracene, which often present coeluting problems. due to the low surface area of the core-shell particles, lowest retention time of each pah was highlighted and effects of the temperature on the separation of pahs were negligible in regard to those using columns packed with fully porous particles. for each pah studied, it was demonstrated that peaks were symmetrical and may be considered as gaussian peaks when the column packed with core-shell particle was employed. in the best condition, the separation of pahs was conducted at 16°c under very low pressure values (670–950 psi = 46–65 bars). depending on pahs, the limit of detection ranged from 0.88 to 9.16 μg l−1. analysis of spiked acetonitrile samples with pahs at 10 and 50 µg l−1 and tap water at 10 µg l−1 gave very good recoveries (94%–109.3%) and high precision (1.1%–3.5%).},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2016a,

title = {Determining the influence of the physicochemical parameters of urban soils on As availability using chemometric methods: A preliminary study},

author = {Christophe Waterlot and Aurélie Pelfrêne and Francis Douay},

year  = {2016},

date = {2016-01-01},

journal = {Journal of Environmental Sciences},

volume = {47},

pages = {183-192},

abstract = {An initial exploration was conducted using mathematical and statistical 

	methods to obtain relevant information about the determination of 

	the physicochemical parameters capable of controlling As uptake by 

	ryegrass grown on contaminated topsoils. Concentrations of As in 

	the soils were from 10 to 47 mg/kg, mainly in the As(V) form (57%–73%). 

	Concentrations of As in water extracts were very low (61–700 μg/kg). 

	It was suggested that As(III) was mainly in the uncharged species 

	and As(V) in the charged species. Chemometric methods revealed that 

	the values of the ratio As(III)/As(V) depended on the assimilated-phosphorus, 

	the pseudo-total and water-extractable Fe contents and the soil pH. 

	Arsenic concentrations measured in ryegrass shoots ranged from 119 

	to 1602 μg/kg. Positive linear correlations were obtained between 

	As in ryegrass shoots and water extractable-As. The transfer coefficient 

	of As correlated well with the ratio assimilated-phosphorus/Fe-oxides. 

	As(III) uptake by the shoot of ryegrass was controlled by the organic 

	matter and Fe-oxide contents.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2016b,

title = {Prediction of extractable Cd, Pb and Zn in contaminated woody habitat soils using a change point detection method},

author = {Christophe Waterlot and Christelle Pruvot and Géraldine Bidar and Clémentine Fritsch and Annette De-Vaufleury and Renaud Scheifler and Francis Douay},

year  = {2016},

date = {2016-01-01},

journal = {Pedosphere},

volume = {26},

number = {3},

pages = {282-298},

abstract = {Accumulation of heavy metals in soils poses a potential risk to plant 

	production, which is related to availability of the metals in soil. 

	The phytoavailability of metals is usually evaluated using extracting 

	solutions such as salts, acids or chelates. The purpose of this study 

	was to identify the most significant soil parameters that can be 

	used to predict the concentrations of acetic and citric acid-extractable 

	cadmium (Cd), lead (Pb) and zinc (Zn) in contaminated woody habitat 

	topsoils. Multiple linear regression models were established using 

	two analysis strategies and three sets of variables based on a dataset 

	of 260 soil samples. The performance of these models was evaluated 

	using statistical parameters. Cation exchange capacity, CaCO3, organic 

	matter, assimilated P, free Al oxide, sand and the total metal concentrations 

	appeared to be the main soil parameters governing the solubility 

	of Cd, Pb and Zn in acetic and citric acid solutions. The results 

	strongly suggest that the metal solubility in extracting solutions 

	is extractable concentration-dependent since models were overall 

	improved by incorporating a change point. This change point detection 

	method was a powerful tool for predicting extractable Cd, Pb and 

	Zn. Suitable predictions of extractable Cd, Pb and Zn concentrations 

	were obtained, with correlation coefficient (adjusted r) ranging 

	from 0.80 to 0.99, given the high complexity of the woody habitat 

	soils studied. Therefore, the predictive models can constitute a 

	decision-making support tool for managing phytoremediation of contaminated 

	soils, making recommendations to control the potential bioavailability 

	of metals. The relationships between acetic and/or citric acid-extractable 

	concentrations and the concentrations of metals into the aboveground 

	parts of plants need to be predicted, in order to make their temporal 

	monitoring easier.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Goulas2015,

title = {Analytical method for determining polycyclic aromatic hydrocarbon pollutants using ultra-fast liquid chromatography with fluorescence detection and the recent column packed with the new 5 μm Kinetex-C18 core-shell particles},

author = {Anaïs Goulas and Brice Louvel and Christophe Waterlot},

year  = {2015},

date = {2015-01-01},

journal = {Canadian Journal of Chemistry},

volume = {93},

number = {5},

pages = {564-571},

abstract = {Nous avons optimisé une méthode de chromatographie liquide à ultra haute performance à détection par fluorescence pour réaliser le dosage de 15 hydrocarbures aromatiques polycycliques (hap) en employant la nouvelle colonne kinetex-c18 (250 mm × 4,6 mm). nous avons utilisé cette colonne récemment commercialisée, contenant un nouveau type de particules poreuses de type core-shell dont la taille moyenne est de 5 μm et fonctionnant à très basse pression, afin de séparer divers composés par chromatographie liquide. après optimisation de la méthode analytique, la séparation des 15 hap dans des échantillons d’eau du robinet dopés a été réalisée, sans coélution de produits, en 21,5 min à 16 °c, dans une phase mobile qui consistait en un mélange aqueux d’acétonitrile, en conditions de gradient de concentrations, à très faible débit (0,7 à 1,0 ml min−1) et à faible pression (870 à 1 590 psi = 60 à 110 bar); toutes ces conditions présentant un intérêt sur le plan économique. pour chacun des composés, nous avons effectué la synchronisation entre les variations de longueur d’onde dans le temps et le temps d’élution dans le but de prévenir une dérive de la ligne de base. nous avons mené la validation de l’ensemble de la procédure expérimentale en tenant compte des paramètres suivants : courbe de calibration, linéarité, limites de détection et de quantification, exactitude, sensibilité, précision et répétabilité du temps de rétention de chaque hap. la méthode analytique proposée présentait une bonne linéarité dans la gamme de concentrations entre 0,025 et 10 μg l−1, où le coefficient de corrélation était supérieur à 0,9980. la répétabilité (écart-type relatif en pour cent},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Janus2015,

title = {Elaboration, characteristics and advantages of biochars for the management of contaminated soils with a specific overview on Miscanthus biochars},

author = {Adeline Janus and Aurélie Pelfrêne and Sophie Heymans and Christophe Deboffe and Francis Douay and Christophe Waterlot},

year  = {2015},

date = {2015-01-01},

journal = {Journal of Environmental Management},

volume = {162},

pages = {275-289},

abstract = {Biochars are products that are rich in carbon obtained by pyrolysis processes that consist in introducing a biomass (such as wood or manure) in a closed container and heating it with little or no available air. this paper reports the impacts of pyrolysis parameters on biochar characteristics. a preliminary examination of the scientific literature revealed that the type of feedstock, the temperature, the heating rate and the gas flow were the major parameters influencing the biochar characteristics. this review highlights the multitude of biochars that can be made and shows the importance of characterizing them before their use in soils. then we assess how the input of biochars in soils can affect soil parameters. a review of the literature showed modifications on: i) the physical properties of soils (i.e. the modification in soil structure and water retention), ii) the chemical properties of soils (i.e. the modification of ph, cation exchange capacity, nutrient availability, the organic matter content) and iii) the biological properties (i.e. the changes in microbial and faunal communities). all these modifications can lead to an increase in crop productivity, which confirms the value of biochars as a soil amendment. moreover, biochars can also provide an advantage for soil remediation. indeed, biochars efficiently reduce the bioavailability of organic and inorganic pollutants. in addition, this review focuses on a specific plant that can be used to produce biochars: miscanthus, a non-wood rhizomatous c4 perennial grass. miscanthus presents advantages for biochar production due to: i) its lignocellulosic content, ii) its silicon content, which can mitigate environmental stresses (notably for plants grown on contaminated sites) and iii) the greater surface area of the miscanthus biochars compared to the biochars produced with other feedstock},

note = {ACLS},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Janus2015a,

title = {Interest of Miscanthus biochars to decrease the bioavailability of metals and PAHs in aqueous solutions},

author = {Adeline Janus and Aurélie Pelfrêne and Sophie Heymans and Christophe Deboffe and Francis Douay and Christophe Waterlot},

year  = {2015},

date = {2015-01-01},

booktitle = {Joint International Biochar Symposium “Understanding Biochar Mechanisms for Pratical Implentation”, 28–30 September 2015, Geisenheim (Allemagne)},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Nsanganwimana2015,

title = {Metal accumulation and shoot yield of Miscanthus × giganteus growing in contaminated agricultural soils: Insights into agronomic practices},

author = {Florien Nsanganwimana and Bertrand Pourrut and Christophe Waterlot and Brice Louvel and Géraldine Bidar and Sonia Labidi and Joël Fontaine and Jérôme Muchembled and Anissa Lounès-Hadj-Sahraoui and Hervé Fourrier and Francis Douay},

year  = {2015},

date = {2015-01-01},

journal = {Agriculture, Ecosystems & Environment},

volume = {213},

pages = {61-71},

abstract = {The choice of agronomic practices for phytomanagement of metal-contaminated soils is of crucial importance to optimize plant biomass yields and to mitigate both environmental and health risks due to metal exposure. the present study aimed to assess the effects of agronomic practices on shoot yield and on metal (cd, pb, and zn) accumulation in the organs of the energy crop miscanthus (miscanthus × giganteus) during the first three years since the plantation on metal-contaminated agricultural soils. three miscanthus cultivars, hereafter named mis-a, mis-b and mis-i, were planted at low and high density. an inoculum of arbuscular mycorrhizal fungi-amf (glomus lpa val 1) was added during plantation, and nitrogen fertilization was applied during the third growing season. metal accumulation in miscanthus organs was determined during the second growing season, whereas shoot yields and their metal concentrations were determined during both the second and the third growing seasons. based on metal concentrations and bioconcentration factors, the three cultivars mainly accumulated metals in their roots. the shoot yields increased from 3.7 to 10.3 t dw ha−1 in the second growing season to 15.8–23.3 t dw ha−1 in third growing season. there were no or very few significant differences in metal concentrations and shoot yields within treatments comprising the same cultivar. the addition of amf inoculum increased metal (mainly cd and zn) accumulation in miscanthus organs and in the shoot yields and this was more observed in both mis-b and mis-i which presented a higher root mycorrhization level than in mis-a. shoot yields in treatments comprising different cultivars depended not on fertilization but on the interactions between cultivar and planting density, and between cultivar, planting density and amf inoculum. whatever the treatment and the sampling period, pb concentrations did not significantly differ in shoot yields. the interaction between cultivar and planting density resulted in higher cd concentrations in the yields of mis-b planted at low density during the third growing season. zn concentrations increased with fertilization in all treatments, and with the addition of the amf inoculum in mis-b and in mis-i. overall, the results demonstrated that the three cultivars could be potential candidates for coupling phytostabilization and biomass production on metal-contaminated soils.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Ouni2015,

title = {Réponses physiologiques et comportementale chez Armadillo officinalis (Duméril, 1816) suite à une contamination du substrat par des ETM},

author = {Ahmed Ouni and Chedliya Ghemari and Christophe Waterlot and Anas Ayari and Francis Douay and Karima Nasri-Ammar},

year  = {2015},

date = {2015-01-01},

booktitle = {16émes journées des sciences de la mer, December 2015, Zarzis (Tunisie)},

abstract = {Armadillo officinalis (duméril, 1816), espèce à large répartition géographique est qualifiée de bio-accumulatrice de métaux lourds. afin de mettre en évidence les réponses physiologiques et comportementales de cette espèce vis-à-vis d’une contamination par des éléments traces métalliques (cd, pb, zn et cu), des expérimentations dans des conditions de laboratoire de température (20° c) et de photopériode (ld16:8) ont été réalisées. pour ce faire, des spécimens en repos sexuel, collectés au niveau des berges de la lagune de ghar el melh, ont été exposés durant 42 jours à des concentrations croissantes des etm cités plus haut. le taux de survie ainsi que l’évolution de la masse pondérale ont été estimés chaque semaine. par ailleurs, un test d’évitement a été réalisé afin de mettre en évidence l’ec50 de cette espèce vis-à-vis des métaux étudiés cd, pb, zn et cu, qui est égale à 2.48, 63.53, 447.63 et 234,05 ppm respectivement},

note = {AFF},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Pelfrene2015a,

title = {Effect of miscanthus cultivation on metal fractionation and human bioaccessibility in metal-contaminated soils: Comparison between greenhouse and field experiments},

author = {Aurélie Pelfrêne and Andrea Kleckerová and Bertrand Pourrut and Florien Nsanganwimana and Francis Douay and Christophe Waterlot},

year  = {2015},

date = {2015-01-01},

journal = {Environmental Science and Pollution Research},

volume = {22},

pages = {3043–3054},

abstract = {The in situ stabilization of metals in soils using plants with great biomass value is a promising, cost-effective, and ecologically friendly alternative to manage metal-polluted sites. the goal of phytostabilization is to reduce the bioavailable concentrations of metals in polluted soil and thus reduce the risk to the environment and human health. in this context, this study aimed at evaluating miscanthus × giganteus efficiency in phytostabilizing metals on three contaminated agricultural sites after short-term exposure under greenhouse conditions and after long-term exposure under field conditions. particular attention was paid to the influence of miscanthus cultivation on (i) cd, pb, and zn fractionation using sequential extractions and (ii) metal bioaccessibility using an in vitro gastrointestinal digestion test. data gave evidence of (i) different behaviors between the greenhouse and the field; (ii) metal redistribution in soils induced by miscanthus culture, more specifically under field conditions; (iii) higher environmental availability for cd than for pb and zn was found in both conditions; and (iv) overall, a higher bioaccessible fraction for pb (about 80 %) and cd (65–77 %) than for zn (36– 52 %) was recorded in the gastric phase, with a sharp decrease in the intestinal phase (18–35 % for cd, 5–30 % for pb, and 36–52 % for zn). compared to soils without culture, the results showed that phytostabilization using miscanthus culture provided evidence for substantial effects on oral bioaccessibility of cd, pb, and zn.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Pelfrene2015b,

title = {Use of an in vitro digestion method to estimate human bioaccessibility of Cd in vegetables grown in smelter-impacted soils: the influence of cooking},

author = {Aurélie Pelfrêne and Christophe Waterlot and Annie Guérin and Nicolas Proix and Antoine Richard and Francis Douay},

year  = {2015},

date = {2015-01-01},

journal = {Environmental Geochemistry and Health},

volume = {37},

pages = {767-778},

abstract = {Metal contamination of urban soils and homegrown vegetables has caused major concern. some studies showed that cadmium (cd) was among the most significant hazards in kitchen garden soils and prolonged exposure to this metal could cause deleterious health effects in humans. in general, most risk assessment procedures are based on total concentrations of metals in vegetables. the present study assesses human bioaccessibility of cd in vegetables cultivated in smelter-impacted kitchen garden soils. seven vegetables (radish, lettuce, french bean, carrot, leek, tomato, and potato) were considered. using the ubm protocol (unified barge bioaccessibility method), the bioaccessibility of cd was measured in raw/cooked vegetables. a considerable amount of cd was mobilized from raw vegetables during the digestion process (on average 85 % in the gastric phase and 69 % in the gastrointestinal phase), which could be attributed to a high uptake of cd during the growth of the vegetables. most cd is accumulated in the vacuoles of plant cells, except what is absorbed by the cell wall, allowing cd to be released from plant tissues under moderate conditions. cooking by the steaming process generally increased the bioaccessibility of cd in french bean, carrot, and leek. for potato, few or no significant differences of cd bioaccessibility were observed after the steaming process, while the frying process strongly decreased bioaccessibility in both phases. the estimation of metal bioaccessibility in vegetables is helpful for human health risk assessment.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Pelfrene2015c,

title = {Evaluation de l'exposition des populations en lien avec la consommation de légumes autoproduits aux alentours d’une ancienne fonderie de plomb: Contribution à l’évaluation des risques sanitaires (BioacLeg)},

author = {Aurélie Pelfrêne and Christophe Waterlot and Nicolas Proix and Annie Guérin and Antoine Richard and Francis Douay},

year  = {2015},

date = {2015-01-01},

booktitle = {Journée Santé Environnement Société, Région Nord – Pas de Calais, 23 juin 2015, Lille},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@phdthesis{Waterlot2015a,

title = {Les éléments métalliques : Intérêt, analyse et devenir dans les sols contaminés : une source potentielle d’innovation en synthèse organique},

author = {Christophe Waterlot},

year  = {2015},

date = {2015-01-01},

school = {HDR Université Lille 1, 358pp},

abstract = {Les éléments métalliques sont souvent mentionnés comme étant indispensables à la synthèse de molécules chimiques en une ou plusieurs étapes. parmi toutes les réactions qui permettent d’illustrer ce constat, citons les réactions d’acylation et d’alkylation de friedel-crafts ou encore les réactions de vinylation de type heck. parallèlement, la contamination des sols par les éléments métalliques est au cœur des préoccupations des instances régionales, nationales et internationales. ceci tient au fait que ces sols présentent des concentrations élevées en éléments métalliques en raison d’une industrialisation et d’une urbanisation très présentes mais aussi, à des comportements variables des éléments métalliques selon leurs spécificités, les paramètres physico-chimiques et biologiques des sols. il en résulte des questionnements en lien avec les problèmes environnementaux et sanitaires et de façon plus globale, des interrogations en lien avec la gestion durable de ces sols. le travail exposé dans mon mémoire porte sur une approche transversale qui pourrait amener in fine à gérer durablement des sols contaminés à partir de matériaux verts et ceci, au service de la synthèse organique. dans un premier volet, il décrit l’intérêt d’utiliser la montmorillonite k10 imprégnée par des éléments métalliques comme d’une part, le zinc dans les réactions de friedel-crafts et d’autre part, le cuivre et le plomb dans les réactions de vinylation des amines aromatiques mais aussi, les effets synergiques entre les éléments métalliques complexés et le motif hydroquinonique présents sur un copolymère régénérable doté de bonnes propriétés en lien avec la réduction du dioxygène dissous dans l’eau. dans un second volet, le mémoire dresse une synthèse des travaux collaboratifs menés au sein du laboratoire génie civil et géo-environnement (lgcge). ils visent à contribuer durablement à la gestion des sols affectés à des degrés divers par deux fonderies de plomb et de zinc connues pour avoir par le passé rejeté des quantités considérables de poussières métalliques dans l’atmosphère. la démarche présentée s’articule autour de 3 axes de recherche : l’analyse des éléments métalliques par spectrométrie d’absorption atomique et les problèmes liés aux interférences spectrales, l’étude du comportement des éléments métalliques dans les sols selon leurs usages (agricoles, urbains et forestiers) au travers de leur mobilité (extractions séquentielles et simples) et de leur phytodisponibilité, l’évaluation des effets de techniques de remédiation (phytostabilisation et/ou immobilisation par voie chimique) sur le comportement des éléments métalliques. le lien qui apparaît entre l’utilisation des éléments métalliques pour la synthèse de macromolécules et ceux présents dans les sols contaminés constitue mon projet de recherche. [...]},

note = {TH HDR},

keywords = {ER4},

pubstate = {published},

tppubtype = {phdthesis}

}

@article{Waterlot2015b,

title = {Arsenic mobility and speciation in contaminated kitchen garden and lawn soils: an evaluation of water for assessment of As phytoavailability},

author = {Christophe Waterlot and Francis Douay},

year  = {2015},

date = {2015-01-01},

journal = {Environmental Science and Pollution Research},

volume = {22},

number = {8},

pages = {6164-6175},

abstract = {Emissions from primary lead smelters have been recognized as one of the mainly factor which has contributed to the contamination of soils by metals. less attention has been paid to volatile metalloids such as arsenic (as) which accompanies lead (pb) smelting activities. one of the objectives of this study was to determine the as concentrations in various extracting solutions using a collection of urban soils located no far away from two former pb and zinc plants in the north of france. the procedure for the determination of as, asiii, and asv with hydride vapor generator atomic absorption spectrometry was described in details. pseudo-total concentrations of as in the studied soils ranged from 5.3 to 65.9 mg kg-1. good correlations were found between as and lead, zinc, and cadmium concentrations in soils. these depended on the soil uses and the soil distance from the source of contamination. because the form of as may pose a health risk to human population, its speciation was determined in each urban top soils. very good correlations were found between asiii and asv versus as concentrations in soils studied, but the results did no permit to establish a relation between the location of soils and their uses. in contrast, it was shown that the highest mobility factor and lowest partitioning index values were related to the location. the mobilty of as depended on the assimilated phosphorus (p), carbonate contents, and ph. the percentages of the water-extractable as concentrations ranged from 0.3 to 3.0 % of the as concentrations in soils. very good positive correlations between water-extractable asiii and asv versus water-extractable as concentrations were obtained. it was shown that the water-extractable asiii concentrations depended on the soil uses. the results revealed that soils for which the as was the most mobile presented the highest water-extractable as concentrations. principal component analysis indicated that mechanisms related to the release of as depended on the physico-chemical parameters of the soils, particularly on the assimilated p, organic matter, and/or iron oxides/hydroxides contents. finally, the glasshouse experiments using ryegrass as plant model and three soils with similar physico-chemical parameters with regard to the pca analysis showed that the water extracting solution could be a good indicator to evaluate the as phytoavailability},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Bidar2014,

title = {Effets à moyen terme d’une phytostabilisation aidée sur la distribution verticale des paramètres physico-chimiques et des éléments traces métalliques dans les sols},

author = {Géraldine Bidar and Brice Louvel and Nicolas Proix and Christophe Waterlot and Hervé Fourrier and Antoine Richard and Francis Douay},

year  = {2014},

date = {2014-01-01},

booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Nsanganwimana2014bb,

title = {Intérêt de Miscanthus × giganteus pour le phytomanagement de sols agricoles contaminés par des éléments traces métalliques dans le Nord de la France},

author = {Florien Nsanganwimana and Bertrand Pourrut and Christophe Waterlot and Géraldine Bidar and Francis Douay},

year  = {2014},

date = {2014-01-01},

booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Nsanganwimana2014c,

title = {Miscanthus x giganteus: une graminée pérenne pour la gestion de sols agricoles contaminés par les éléments trace métalliques dans le nord de la France},

author = {Florien Nsanganwimana and Bertrand Pourrut and Christophe Waterlot and Francis Douay},

year  = {2014},

date = {2014-01-01},

booktitle = {Journée de l'environnement, LaSalle Beauvais, 27 mars 2014, Beauvais},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Nsanganwimana2014d,

title = {Comportement de Miscanthus cultivé sur des sols fortement contaminés en Cd, Pb et Zn},

author = {Florien Nsanganwimana and Christophe Waterlot and Géraldine Bidar and Bertrand Pourrut and Francis Douay},

year  = {2014},

date = {2014-01-01},

booktitle = {Gestion et requalification durable des sites et sols pollués : Expériences en Nord - Pas de Calais, 25-26 septembre 2014, Villeneuve d’Ascq},

abstract = {Objectifs spécifiques de l’étude §evaluer l’aptitude de m. × giganteus à se developer sur les sols contaminés §etudier l’influence de la culture sur les paramètres physico-chimiques des sols et le comportement de cd, pb et zn §déterminer l’accumulation des etm dans les organes de m. × giganteus selon le gradient de contamination des sols et les pratiques culturales},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pelfrene2014,

title = {Evaluation de l'impact de deux filières de phytomanagement sur la bioaccessibilité orale de Cd et Pb pour l’homme},

author = {Aurélie Pelfrêne and Christophe Waterlot and Francis Douay},

year  = {2014},

date = {2014-01-01},

booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris},

abstract = {Dans une démarche de gestion des sites et sols pollués, la requalification des sols par le phytomanagement permet de limiter les risques environnementaux et de maintenir une activité économique. le mode de gestion proposé dans la présente étude implique deux filières de phytomanagement à l’aide (1) d’une graminée vivace (miscanthus x giganteus) et (2) d’essences arborées avec et sans ajout d’amendements minéraux. en vue de mieux évaluer les dangers sanitaires en lien avec l’ingestion de particules de sols contaminés, la bioaccessibilité orale de cd et pb a été déterminée sur des sols collectés avant et après leur requalification. l’intérêt d’introduire la notion de bioaccessibilité au sein de l’évaluation de risques, au lieu des concentrations totales en métaux, est d’obtenir une valeur d’exposition plus pertinente. globalement, les modes de gestion proposés ne permettent pas de diminuer la bioaccessibilité orale de cd et pb dans la phase gastrique. dans la phase gastro-intestinale, qui d’un point de vue physiologique correspond à la phase la plus pertinente, la bioaccessibilité de cd n’est pas diminuée. en revanche, le phytomanagement (principalement avec le miscanthus) permet la réduction de la bioaccessibilité orale pour l’homme de pb.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pelfrene2014a,

title = {Use of an in vitro digestion method to estimate cadmium bioaccessibility in vegetables grown in smelter-impacted soils: influence of cooking},

author = {Aurélie Pelfrêne and Christophe Waterlot and Nicolas Proix and Annie Guérin and Antoine Richard and Francis Douay},

year  = {2014},

date = {2014-01-01},

booktitle = {30th International SEGH conference, 30 June – 4 July 2014, Newcastle upon Tyne (UK)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pourrut2014a,

title = {Miscanthus x giganteus: a promising perennial grass for sustainable phytomanagement of heavy metal contaminated sites in Northern France},

author = {Bertrand Pourrut and Florien Nsanganwimana and Christophe Waterlot and Francis Douay},

year  = {2014},

date = {2014-01-01},

booktitle = {Sustainable Remediation Conference 2014, 17-19 September 2014, Ferrara (Italie)},

abstract = {Soil contamination by metals is of major concern in northern france, particularly in the former coal-mining region. metaleurop nord, located at noyelles-godault, was a major european lead (pb) and zinc (zn) smelter for almost a century until its closure in 2003. in this area, the mean concentrations of cd, pb and zn in ploughed layers are 20-50 times higher than the regional background values and their concentrations in agricultural crops exceed threshold values for human consumption. contaminated dusts are also a risk for human health, especially children. the remediation of the highly contaminated soils is a major preoccupation for national and local authorities. however, the contaminated area is too large to be remediated in an economically relevant way by the currently applied remediation techniques. moreover, in order to find new income sources for local farmers, a sustainable management of these polluted soils is crucial. in this context, the phytener project aims to assess phytostabilization in combination with energy crop production (wood and miscanthus crops). the phytener consortium is a strong region-wide network of 11 laboratories, which leads a multidisciplinary approach combining a study of the impacts of these crops on soils and the environment, on social perceptions and on economic outcomes. in spring 2007, three approximately-1 hectare miscanthus giganteus experimental fields were established on former agricultural lands presenting a contamination gradient. the aim of this study was to (a) evaluate the ability of this plant to grow on metal-contaminated soils, b) to establish metal accumulation patterns in m. giganteus organs and c) to study the relationship between metal accumulation in the plant organs and their fractionations in soils. topsoil and plant samplings were carried out in september 2011. in field experimental conditions, the results show that m. giganteus grows well on highly contaminated soils. measured metal concentrations, bioconcentration and transfer factors demonstrate that this species accumulates metals mainly in roots and strongly limits their transfer to aboveground parts. in roots and rhizomes, the accumulation was as follows: cd > zn > pb. conversely, in stems and leaves it was zn ≥ cd > pb. though soils present a clear contamination! gradient, there were no significant difference in metal concentrations in stems and leaves. therefore, m. giganteus could be considered as a metal-excluder plant and appears to be a potential candidate crop for coupling phytostabilization and production of a valuable biomass on contaminated sites.},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Waterlot2014,

title = {Intérêt de la méthode de correction du bruit de fond par renversement de spectre lors du dosage par spectrométrie d’absorption atomique électrothermique de l’arsenic dans des matrices contenant des concentrations élevées en aluminium},

author = {Christophe Waterlot and Francis Douay and Fabien Daurangeon},

year  = {2014},

date = {2014-01-01},

journal = {Spectra Analyse},

volume = {297},

pages = {52-56},

note = {ACLN},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2014a,

title = {Réduction des effets de matrices et des interférences lors du dosage de l’antimoine par spectrométrie d’absorption atomique électrothermique},

author = {Christophe Waterlot and Francis Douay and Fabien Daurangeon},

year  = {2014},

date = {2014-01-01},

journal = {Spectra Analyse},

volume = {297},

pages = {58-63},

note = {ACLN},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Waterlot2014b,

title = {Évaluation des effets de Miscanthus × giganteus sur la distribution des ETM d’une parcelle agricole},

author = {Christophe Waterlot and Aurélie Pelfrêne and Florien Nsanganwimana and Bertrand Pourrut and Francis Douay},

year  = {2014},

date = {2014-01-01},

booktitle = {3èmes Rencontres nationales de la Recherche sur les sites et sols pollués, ADEME, 18-19 novembre 2014, Paris},

abstract = {Le phytomanagement peut être un mode de gestion de sols contaminés en visant à limiter les dangers environnementaux et sanitaires mais aussi, à requalifier les milieux dégradés en restaurant leurs fonctions. l’un des modes de gestion proposé dans le cadre du programme phytener repose sur l’utilisation, à des fins de production d’une biomasse, d’une graminée vivace et pérenne, miscanthus × giganteus. dans le but d’étudier les effets du couvert végétal sur la mobilité des métaux, des extractions séquentielles ont été réalisées avant et après son installation. bien que la mobilité de cd, pb et zn ait significativement diminué la première année qui a suivi la mise en place du dispositif expérimental, il s’avère que l’extractabilité de cd et pb reste globalement inchangée à l’issue de trois années d’expérimentation. en revanche, miscanthus × giganteus induit une augmentation de la mobilité de zn.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Ghemari2014,

title = {Metal bioaccumulation in two species of Oniscideans: Porcellio laevis and Porcellionides pruinosus from Tunisian contaminated sites},

author = {Chedliya Ghemari and Christophe Waterlot and Julie Leclercq-Dransart and Francis Douay and K Nasri-Ammar},

year  = {2014},

date = {2014-01-01},

booktitle = {9th International Symposium of Terrestrial Isopod Biology, 26-30 juin 2014, Poitiers},

abstract = {Terrestrial isopods that are important decomposers, are known for their resistance to high soil contamination by metal trace, making their use very helpful in biomonotoring. the aim of the present study was to compare metal bioaccumulation of two common species of porcellio laevis and porcellionides pruinosus exposed to the same contaminated soil belonging to industrial area polluted by metal trace elements in the north (1), central (3) and south (1) of tunisia. we hypothesized that these two species would accumulate metals according to their bioavailability in soil and in vegetation. these results will be discussed in relation to the soil and climatic of the different sites explored},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Goulas2013,

title = {Optimisation des conditions d’analyses des HAP de la liste US EPA en UFLC munie d’un détecteur à barrettes de diodes et d’une colonne Kinetex®- C18 à particules Core-Shell},

author = {Anaïs Goulas and Aurélie Pelfrêne and Francis Douay and Christophe Waterlot},

year  = {2013},

date = {2013-01-01},

journal = {Spectra Analyse},

volume = {295},

pages = {48-53},

abstract = {Les hydrocarbures aromatiques polycycliques (hap) sont présents dans les compartiments environnementaux (sols, sédiments, particules en suspension dans l’eau et dans l’air) et dans la chaîne alimentaire. connus pour leurs effets mutagènes, génotoxiques et cancérogènes, les hap font l’objet de nombreuses études portant sur la caractérisation des dangers qu’ils représentent, soit individuellement, soit en mélange. quelles que soient les matrices contaminées, la quantification à l’état de traces et d’ultratraces des hap nécessite des techniques analytiques permettant une séparation des composés avec une sensibilité optimale. les caractéristiques analytiques d’un système associant une chaîne uflc (ultra fast liquid chromatography), un détecteur à barrettes de diodes et une colonne équipée des particules core- shell ont donc été déterminées pour l’analyse des 16 hap classés prioritaires par l’agence de protection de l’environnement américaine (us epa). leur séparation a été réalisée en 21,5 minutes et un bon compromis entre ce temps et la résolution des pics a permis l’intégration automatisée de l’ensemble des hap au point d’obtenir des limites de détection faibles (comprises entre 0,88 et 9,16 μg.l-1) au regard du détecteur utilisé.},

note = {ACLN},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Goulas2013a,

title = {Optimisation des conditions d’analyses des HAP en UFLC équipée d’un détecteur à barrettes de diodes et d’une colonne Kinetex®- C18},

author = {Anaïs Goulas and Aurélie Pelfrêne and Francis Douay and Christophe Waterlot},

year  = {2013},

date = {2013-01-01},

booktitle = {Université Catholique de Lille - Journée de la R&D en développement durable et responsabilité sociétale, 31 mai 2013, Lille},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Pelfrene2013a,

title = {Influence of land use on human bioaccessibility of metals in smelter-impacted soils},

author = {Aurélie Pelfrêne and Christophe Waterlot and Francis Douay},

year  = {2013},

date = {2013-01-01},

journal = {Environmental Pollution},

volume = {178},

pages = {80-88},

abstract = {An investigation was undertaken to evaluate the empirical model developed by pelfrene et al. (2012), predicting the human bioaccessibility of cd and pb in smelter-contaminated agricultural topsoils, by including other soil uses: 50 urban and 65 woody habitat topsoils collected in the same area. the results showed that land use significantly affected the pseudototal metal concentrations and their oral bioaccessibility. however, whatever the soil's physicochemical parameters and degree of contamination, the 'agricultural' model can be used to simulate metal gastric bioaccessibility in urban and woody habitat soils. to simulate gastrointestinal bioaccessibility, this model can be used directly if the pseudototal metal concentrations are on the same order of magnitude as those usually recorded in the agricultural soils studied or after the use of a correction factor if these concentrations are greater. these results showed that the oral bioaccessibility predictions could be applicable for further environmental risk evaluation. (c) 2013 elsevier ltd. all rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Stocker2013,

title = {On the synthesis and biological properties of isocombretastatins: a case of ketone homologation during Wittig reaction attempts},

author = {Vivien Stocker and Alina Ghinet and Marie Leman and Benoit Rigo and Régis Millet and Amaury Farce and Déborah Desravines and Joëlle Dubois and Christophe Waterlot and Philippe Gautret},

year  = {2013},

date = {2013-01-01},

journal = {RSC Advances},

volume = {3},

pages = {3683-3696},

abstract = {New isocombretastatins were synthesized by reacting the corresponding phenstatin analogs with ch3pph3br in presence of tbuok. these new derivatives showed significant activities against cellular proliferation and tubulin polymerization. in particular, monomethoxylated derivatives of phenstatin and isoca-4 exhibit similar activities to those of parent phenstatin. attempts of the wittig reaction on 2- (or 4-) methoxy-4′-nitrobenzophenones in the same conditions do not lead to the expected isocombretastatins but to methyleneketones with the exclusion of triphenylphosphine. a mechanism for this new ketone homologation was proposed},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2013,

title = {Contamination, fractionation and availability of metals in urban soils in the vicinity of former lead and zinc smelters, France},

author = {Christophe Waterlot and Géraldine Bidar and Aurélie Pelfrêne and Hélène Roussel and Hervé Fourrier and Francis Douay},

year  = {2013},

date = {2013-01-01},

journal = {Pedosphere},

volume = {23},

number = {2},

pages = {143-159},

abstract = {Soil contamination by metals from anthropogenic activities (e.g., mining and smelting) is a major concern for the environment and human health. environmental availability of cadmium (cd), lead (pb), zinc (zn), copper (cu), and indium (in) in 27 urban soils located around two former pb and zn smelters in northern france were studied by analysing the chemical forms of these metals and evaluating their phytoavailability. these metals were determined using flame or electrothermal absorption atomic spectrometry (faas or etaas), depending on their concentration levels. after optimisation of the etaas method, characteristic mass of in in water and aqua regia were 9.9 and 18 pg, respectively, showing the high sensitivity of the analytical procedure. metal partitioning was conducted using a four-step sequential extraction procedure. the results showed that cd and zn were mainly in the acid-extractable and reducible forms in the urban soils studied. in contrast, pb and in were largely in the reducible fraction. however, in some samples, the amount of in extracted in the residual or exchangeable fraction was higher than that in the reducible fraction. copper was mainly found in the reducible and residual fractions. a pot experiment was conducted in a glasshouse with seven soils (six contaminated and one uncontaminated) and two plant species, ryegrass and lettuce. the results showed transfer of metals from the contaminated soils to the shoots of ryegrass and the edible part of lettuce. the metal bioconcentration factor was in the order of cd >> cu > in > zn >> pb for lettuce leaves, whereas for ryegrass shoots, three orders were found, cd > zn > cu >> in > pb, cd >= in > zn > cu >> pb, and zn > cd > cu > in > pb, depending on the physico-chemical properties of the soils, such as ph, cation exchange capacity, carbonates, and organic matter. it was established that the metal toxicity was related to the contamination levels and the physico-chemical properties, including ph, organic matter, and in a lesser extent, ca, mg, and phosphorus contents, of the soils. however, it was shown that lettuce could grow on soils having high cd and caco3 contents. cadmium was one of the most available metals while pb was always the least available in the soils studied.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2013a,

title = {Evaluation des effets d’une lampe à cathode creuse pulsée à courant variable sur les interférences spectrales de l’arsenic dans le dosage du cadmium par spectrophotométrie d’absorption atomique},

author = {Christophe Waterlot and Francis Douay},

year  = {2013},

date = {2013-01-01},

journal = {Canadian Journal of Civil Engineering},

volume = {8},

number = {4},

pages = {485-492},

abstract = {Les teneurs en cadmium de solutions aqueuses contaminees a des teneurs croissantes en arsenic ont ete mesurees a l’aide d’un spectrophotome`tre d’absorption atomique equipe d’une flamme et d’un four electrothermique. pour chacune des techniques d’atomisation, a ete evaluee une me´thode de correction du bruit de fond base´e sur le renversement de spectre et connue sous le nom de « high-speed self-reversal method », au regard d’une autre methode plus couramment utilisee ne ne´cessitant qu’une lampe au deute´rium et une lampe au cadmium. en comparaison avec cette me´thode, les re- sultats ont montre que le mode de correction base´ sur le renversement spectral via l’utilisation d’une lampe au cadmium a` cathode creuse pulse´e a` courant variable etait une methode plus approprie´e pour corriger les phe´nome`nes d’interfe´rences causes par l’arsenic (as) lors du dosage du cadmium (cd). il a ete etabli que cette methode permettait de s’affranchir des interferences de l’arsenic pour des ratios [as]/[cd] infe´rieurs a` 50 000.},

note = {ACLN},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2013b,

title = {Minimizing matrix effects and spectral interferences produced by Fe absorption lines in the determination of cadmium by electrothermal atomic absorption spectrometry: Application to the fractionation of cadmium in moderated contaminated soils},

author = {Christophe Waterlot and Francis Douay},

year  = {2013},

date = {2013-01-01},

journal = {Measurement},

volume = {46},

pages = {2348-2358},

abstract = {Solutions, including the extracting solutions used in the sequential procedure recommended by the standard, measurements and testing program, with different cadmium and iron concentrations were prepared and analyzed using combined electrothermal atomic absorption spectrometry (etaas) and two background compensation techniques. the first was based on the deuterium background correction method in which a deuterium lamp was used in combination with a conventional cd hollow-cathode lamp. for the second, a high-intensity boosted discharge hollow-cathode lamp (bdhcl) was used as in the smith–hieftje (s–h) background correction method, but this lamp was modulated with a low and a high current mode at a modulation frequency of 100 hz. in the present study, the modulation cycle was 10 times higher than in the s–h system. therefore, the background correction method was called high-speed self-reversal method. pseudo-total cd concentrations were determined using etaas in five reference materials and fractionation was made in two reference soils. with regard to the results, the high-speed self-reversal background correction method provided a method of choice to eliminate or decrease the spectral interference due to the close positioning of the analytical lines of cd and fe in solutions containing high iron concentrations during the cd determination by electrothermal atomic absorption spectrometry. it is worth noting that using the hssr-method, the sensitivity losses were from 32% to 39% compared to the continuum source background corrector equipped with a deuterium lamp. the hssr-method was successfully applied to the determination of cd in contaminated samples. the results showed that interferences caused by absorption line overlapping of cd and fe could be compensated accurately for fe/cd = 100,000 in 0.11 m acetic acid and fe/cd < 10,000 in 0.5 m hydroxylamine hydrochloride.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Godet2012,

title = {Fluctuating asymmetry analysis on Porcellio scaber (Crustacea, Isopoda) populations living under metals-contaminated woody habitats},

author = {Jean-Philippe Godet and Sylvain Demuynck and Christophe Waterlot and Sébastien Lemière and Catherine Souty-Grosset and Francis Douay and Alain Leprêtre and Christelle Pruvot},

year  = {2012},

date = {2012-01-01},

journal = {Ecological Indicators},

volume = {23},

pages = {130-139},

abstract = {This field study aimed at determining the effect of metal-polluted environments on fluctuating asymmetry (fa) level of porcellio scaber as a measure of developmental stability (ds). for this, woodlice and litter were collected on 8 sites of northern france located either away from metal pollution source or near metallurgical smelters. physico-chemical parameters such as ph, c/n ratio and caco(3) were measured in litter since they could have potential effects on woodlouse population. moreover, cd, pb, and zn concentrations in litter were determined to evaluate the contamination degree of each site. the metal body burdens were determined in woodlice to evaluate their impregnation degree. fluctuating asymmetry levels on males and gravid females were performed on 9 traits (the 2nd and the 3rd articles of antennae, the 1st flagellum segment of the antennae, and the merus, carpus and propodus of the 6th and the 7th pereiopods) according (1) to each site and (2) to two habitat types (cf. metal-contaminated and uncontaminated habitats). results showed close correlations between litter metal concentrations and metal body burdens in woodlice. however, contrary to the initial hypothesis that metal pollution would induce an increase of the fa level, this seemed to be lower for individuals from metal-contaminated habitats than for those from uncontaminated habitats, and particularly for males. the role of antennae and pereiopods on the foraging behavior and reproduction processes on woodlice were discussed and could explain these observations. (c) 2012 elsevier ltd. all rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Pelfrene2012b,

title = {Validation and first deployment of the DGT technique in artificial human gastrointestinal fluids after ingestion of metal-containing soil particles},

author = {Aurélie Pelfrêne and Christophe Waterlot and Francis Douay},

year  = {2012},

date = {2012-01-01},

booktitle = {SETAC. 20-24 May 2012, Berlin (Allemagne)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Pelfrene2012c,

title = {Modélisation de la bioaccessibilité orale de Cd et Pb au regard des paramètres physico-chimiques de sols agricoles fortement contaminés : Application à un site contaminé du Nord-Pas de Calais},

author = {Aurélie Pelfrêne and Christophe Waterlot and Muriel Mazzuca and Catherine Nisse and Géraldine Bidar and Francis Douay},

year  = {2012},

date = {2012-01-01},

booktitle = {Intersol. 27-30 mars 2012, Paris},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Pelfrene2012a,

title = {Bioaccessibility of trace elements as affected by soil parameters in smelter-contaminated agricultural soils: A statistical modeling approach},

author = {Aurélie Pelfrêne and Christophe Waterlot and Muriel Mazzuca and Catherine Nisse and Damien Cuny and Antoine Richard and Sébastien Denys and Christophe Heyman and Hélène Roussel and Géraldine Bidar and Francis Douay},

year  = {2012},

date = {2012-01-01},

journal = {Environmental Pollution},

volume = {160},

pages = {130-138},

abstract = {An investigation was undertaken to identify the most significant soil parameters that can be used to predict cd, pb, and zn bioaccessibility in smelter-contaminated agricultural soils. a robust model was established from an extended database of soils by using: (i) a training set of 280 samples to select the main soil parameters, to define the best population to be taken into account for the model elaboration, and to construct multivariate regression models, and (ii) a test set of 110 samples to validate the ability of the regression models. total carbonate, organic matter, sand, p2o5, free feemn oxide, and pseudototal al and trace element (te) contents appeared as the main variables governing te bioaccessibility. the statistical modeling approach was reasonably successful, indicating that the main soil factors influencing the bioaccessibility of tes were taken into account and the predictions could be applicable for further risk evaluation in the studied area.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2012,

title = {Effects of grinding and shaking on Cd, Pb and Zn distribution in anthropogenically impacted soils},

author = {Christophe Waterlot and Géraldine Bidar and Christelle Pruvot and Francis Douay},

year  = {2012},

date = {2012-01-01},

journal = {Talanta},

volume = {98},

pages = {185-196},

abstract = {The effectsofgrinding size andshaking process on the results ofcd (cadmium), pb (lead) and zn (zinc) distribution measurements three agricultural and three kitchen garden soils highly contaminated by past atmospheric fallout of two lead and zinc smelters in northern france were studied. the physico-chemical parameters and pseudo-total concentration of metals within these soils were determined. the fractionation of metals was performed in triplicate, using the procedure recommended by the standards, measurements and testing program (sm&t), on each air-dried soil sample, ground to pass through 2-mm, 0.315-mm and 0.250-mm sieves and using a reciprocating or rotary shaker. the samples were analysed by flame or electrothermal absorption atomic spectrometry using a self-reversal background system. for both shaking processes, the grinding size had no effecton the fractionation of metals in contaminated agricultural soils. in contrast, using a reciprocating shaker, the fractionation of metals in the kitchen garden samples sieved at <2 mm was so different that in the samples prepared to pass through the 0.315-mm and 0.250-mm sieves. therefore changes (use of a 50 ml graduated polypropylene centrifuge tube, evaporation of the solution to a fixed volume in step 3 and the use of an automatic shaking heating bath) were made to the initial procedure and a rotary shaker was used to improve the suspension of the soil samples during extraction. for all grinding sizes, the fractionation of the three metals contained in the contaminated kitchen garden soil samples was successfully achieved. nevertheless, some discrepancies from samples sieved at <2 mm were obtained. on the other hand, it is worth noting that the effectof the type of shaker on the distribution of metal depended on the soil and the grinding size. from an analytical point of view, precision and trueness were improved after optimisation of the procedure for all sequential extraction procedure steps. the best results were obtained for samples sieved at <0.250 mm. similar trends were obtained using the crm bcr®-701 certified material. all the results showed that optimisation of the sequential extraction procedure could be adopted for the cd-, pb- and zn-partitioning in contaminated kitchen garden soils with high-level anthropogenic sources.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2012a,

title = {Minimizing Chloride Interferences Produced by Calcium Chloride in the Determination of Cd by Graphite Furnace Atomic Absorption Spectrometry},

author = {Christophe Waterlot and Francis Douay},

year  = {2012},

date = {2012-01-01},

journal = {ISRN Spectroscopy},

pages = {1-10},

abstract = {Cadmium concentrations in cacl2 extracting solutions at various concentrations were determined by graphite furnace atomic absorption using two background correctors: the deuterium and the high-speed self-reversal background correction systems. under- and overestimation of the cd absorbance signals in cacl2 solutions were observed for concentrations greater than 0.005m using the deuterium lamp while no important effect was observed using the other background correction system. the analytical performance of the spectrometer for the determination of cd was studied in 0.01m cacl2 solution and single extractions were performed using reference materials and contaminated soil samples. cadmium was determined using the two background correction systems and a third method, which consists of the use of the deuterium lamp without any chemical modifier, was added to the study. the results showed that the thirdmethod was unable to determine cd concentrations in the cacl2 solution due to the presence of extractable arsenic and iron. for solutions without any dilution or diluted with a very low dilution factor, the cacl2- extractable cd concentrations measured using the deuterium lamp were systematically below those found using the high-speed self-reversal method. these differences were explained by the presence of chloride ions in the atomization step.},

note = {ACLO},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2012b,

title = {La spectrométrie d’absorption atomique par électrothermie: une technique d’analyse simple pour la détermination de l’indium dans les sols contaminés},

author = {Christophe Waterlot and Francis Douay and Fabien Daurangeon},

year  = {2012},

date = {2012-01-01},

journal = {Spectra Analyse},

volume = {289},

pages = {24-28},

note = {ACLN},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2012c,

title = {Application of the high-speed self-reversal background corrector to the determination of cadmium by chemical vapor generation atomic absorption spectrometry},

author = {Christophe Waterlot and Aurélie Pelfrêne and Francis Douay},

year  = {2012},

date = {2012-01-01},

journal = {Canadian Journal of Chemistry},

volume = {90},

pages = {1-6},

abstract = {Les concentrations en cadmium (cd) dans les solutions d’extraction (sels neutres) de sols contaminés sont souvent trop faibles pour pouvoir être déterminées par spectrométrie d’absorption atomique en utilisant une simple flamme pour atomiser les éléments. la quantification de cd a` l’état de traces requière donc des méthodes d’analyses sensibles mais aussi exemptes d’interférences. dans ce contexte, le dosage de cd a été réalisé au moyen d’un spectromètre d’absorption atomique couplé a` un générateur d’hydrure en mode continu et combiné a` une méthode de correction du bruit de fond par renversement de spectre. les conditions optimales d’utilisation de la technique ont été déterminées de manière a` obtenir une sensibilité maximale. pour des concentrations de 2,5 et 5 ng ml–1, les absorbances maximales de cd ont été obtenues en préparant une solution de nabh4 a` 3 % dans une solution de soude a` 1,5 % et en analysant des solutions dont la concentration en hno3 a été de 0,3 mol/l. dans ces conditions, la limite de détection a été de 1 ng ml–1 et un coefficient de variation de 5 % a été trouvé en analysant a` 10 reprises des solutions de cd a` 0,2 et 1 ng ml–1, montrant ainsi une bonne sensibilité et reproductibilité de la méthode. de plus, la méthode analytique proposée s’est avérée être une méthode efficace permettant de minimiser les interférences causées par les cations as, al, ca, fe, mn, ni, pb, se et zn jusqu’a` 10 g ml–1. la méthode a été validée par la détermination des concentrations en cd dans les solutions d’extraction au cacl2 0,01 mol/l de sols certifiés et de sols contaminés. les données acquises avec la méthode proposées ont été comparées a` celles obtenues en utilisant des méthodes d’analyses plus conventionnelles, spectrométrie absorption atomique avec atomisation électrothermique et flamme. l’ensemble des résultats obtenus a montré que la méthode proposée était sensible, reproductible et permettait de s’affranchir de certaines interférences.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2012d,

title = {Effects of iron concentration level in extracting solutions from contaminated soils on the determination of zinc by flame atomic absorption spectrometry with two background correctors},

author = {Christophe Waterlot and Aurélie Pelfrêne and Francis Douay},

year  = {2012},

date = {2012-01-01},

journal = {Journal of Analytical Methods in Chemistry},

pages = {1-10},

abstract = {Zinc and iron concentrations were determined after digestion, water, and three-step sequential extractions of contaminated soils. analyses were carried out using flame absorption spectrometry with two background correctors: a deuterium lamp used as the continuum light source (d2 method) and the high-speed self-reversal method (hssr method). regarding the preliminary results obtained with synthetic solutions, the d2 method often emerged as an unsuitable configuration for compensating iron spectral interferences. in contrast, the hssr method appeared as a convenient and powerful configuration and was tested for the determination of zinc in contaminated soils containing high amounts of iron. simple, fast, and interference-free method, the hssr method allows zinc determination at the ppb level in the presence of large amounts of iron with high stability, sensitivity, and reproducibility of results. therefore, the hssr method is described here as a promising approach for monitoring zinc concentrations in various iron-containing samples without any pretreatment},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Brulle2011,

title = {Gene expression analysis of 4 biomarker candidates in Eisenia fetida exposed to an environmental metallic trace elements gradient: A microcosm study},

author = {Franck Brulle and Sébastien Lemière and Christophe Waterlot and Francis Douay and Franck Vandenbulcke},

year  = {2011},

date = {2011-01-01},

journal = {Science of the Total Environment},

volume = {409},

number = {24},

pages = {5470-5482},

abstract = {Past activities of 2 smelters (metaleurop nord and nyrstar) led to the accumulation of high amounts of metal trace elements (tes) in top soils of the noyelles-godault/auby area, northern france. earthworms were exposed to polluted soils collected in this area to study and better understand the physiological changes, the mechanisms of acclimation, and detoxification resulting from te exposure. previously we have cloned and transcriptionally characterized potential biomarkers from immune cells of the ecotoxicologically important earthworm species eisenia fetida exposed in vivo to te-spiked standard soils. in the present study, analysis of expression kinetics of four candidate indicator genes (cadmium-metallothionein, coactosin like protein, phytochelatin synthase and lysenin) was performed in e. fetida after microcosm exposures to natural soils exhibiting an environmental cadmium (cd) gradient in a kinetic manner. te body burdens were also measured. this microcosm study provided insights into: (1) the ability of the 4 tested genes to serve as expression biomarkers, (2) detoxification processes through the expression analysis of selected genes, and (3) influence of land uses on the response of potential biomarkers (gene expression or te uptake). (c) 2011 elsevier b.v. all rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Douay2011b,

title = {Impacts d’un mode de phytomanagement sur des sols fortement contaminés par des métaux},

author = {Francis Douay and Alena Lopareva-Pohu and Bertrand Pourrut and Christophe Waterlot and Guillaume Garçon and Géraldine Bidar and Christelle Pruvot and Pirouz Shirali},

year  = {2011},

date = {2011-01-01},

booktitle = {ValBiom, 8èmes rencontres de la Biomasse. 26 octobre 2011, Gembloux (Belgique)},

note = {COM},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@conference{Douay2011c,

title = {Fly ash-aided phytostabilisation of highly contaminated soils: assessment of field trial after 8 years},

author = {Francis Douay and Bertrand Pourrut and Christophe Waterlot and Alena Lopareva-Pohu and Christelle Pruvot and Guillaume Garçon and Géraldine Bidar and Pirouz Shirali},

year  = {2011},

date = {2011-01-01},

booktitle = {11th International Conference on the Biogeochemistry of Trace Elements (ICOBTE). 3-7 July 2011, Florence (Italie)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Godet2011,

title = {Growth and metal accumulation in Porcellio scaber exposed to poplar litter from Cd-, Pb- and Zn-contaminated sites},

author = {Jean-Philippe Godet and Sylvain Demuynck and Christophe Waterlot and Sébastien Lemière and Catherine Souty-Grosset and Renaud Scheifler and Francis Douay and Alain Leprêtre and Christelle Pruvot},

year  = {2011},

date = {2011-01-01},

journal = {Ecotoxicology and Environmental Safety},

volume = {74},

number = {3},

pages = {451-458},

abstract = {This study aimed at determining, in the laboratory, the effects of poplar litter collected in woody habitats contaminated by heavy metals on growth and metal accumulation in the terrestrial isopod porcellio scaber. cd, pb, and zn pseudototal and cacl2-extractable concentrations in litter types were determined using aas. juveniles were fed ad libitum, individually, for 28 days with four litter types presenting an increasing gradient of metal contamination. individuals were weighed every week and metal body burdens were determined at the end of the experiment. from the first week until the end of the experiment, a decrease in p. scaber growth related to the increase of metal concentration in litter types was recorded. significant correlations were observed between metal body burdens and metal concentrations in litter types. however, cd accumulation in woodlice appeared to be related to the cd/zn concentration ratio in litter types. all these results showed the potential of weight gain in p. scaber as a suitable indicator for litter quality assessment with ecological relevance.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Lopareva-Pohu2011b,

title = {Assessment of fly ash-aided phytostabilisation of highly contaminated soils after an 8-year field trial Part 1. Influence on soil parameters and metal extractability},

author = {Alena Lopareva-Pohu and Bertrand Pourrut and Christophe Waterlot and Guillaume Garçon and Géraldine Bidar and Christelle Pruvot and Pirouz Shirali and Francis Douay},

year  = {2011},

date = {2011-01-01},

journal = {Science of the Total Environment},

volume = {409},

number = {3},

pages = {647-654},

abstract = {Sustainable management of large surface areas contaminated with trace elements is a real challenge, since currently applied remediation techniques are too expensive for these areas. aided phytostabilisation appears to be a cost efficient technique to reduce metal mobility in contaminated soils and contaminated particle spread. in this context, this study aimed at evaluating the long-term efficiency of aided phytostabilisation on former agricultural soils highly contaminated with trace elements. the influence of afforestation and fly ash amendments to reduce metal mobility was investigated. before being planted with a tree mix, the study site was divided into three plots: a reference plot with no amendment, the second amended with silico-aluminous fly ash and the third with sulfo-calcic fly ash. after eight years, some soil physico-chemical parameters, including cadmium (cd), lead (pb) and zinc (zn) extractability were modified. in particular, ph decreased on the whole site while organic carbon content increased. the alteration of these parameters influencing trace element mobility is explained by afforestation. over time, concentrations of cacl2-extractable metals increased and were correlated with the soil ph decrease. in the amended soils, extractable cd, pb and zn concentrations were lower than in the reference soil. the results indicated that the two fly ashes buffered natural soil acidification due to vegetation development and limited trace element mobility and thus could limit their bioavailability. for long-term phytostabilisation, special attention should be focused on the soil ph, metal mobility and phytoavailability analysis. (c) 2010 elsevier b.v. all rights reserved.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Lopareva-Pohu2011a,

title = {Influence of fly ash aided phytostabilisation of Pb, Cd and Zn highly contaminated soils on Lolium perenne and Trifolium repens metal transfer and physiological stress},

author = {Alena Lopareva-Pohu and Anthony Verdin and Guillaume Garçon and Anissa Lounès-Hadj-Sahraoui and Bertrand Pourrut and Djouher Debiane and Christophe Waterlot and Frédéric Laruelle and Géraldine Bidar and Francis Douay and Pirouz Shirali},

year  = {2011},

date = {2011-01-01},

journal = {Environmental Pollution},

volume = {159},

number = {6},

pages = {1721-1729},

abstract = {Due to anthropogenic activities, large extends of soils are highly contaminated by metal trace element (mte). aided phytostabilisation aims to establish a vegetation cover in order to promote in situ immobilisation of trace elements by combining the use of metal-tolerant plants and inexpensive mineral or organic soil amendments. eight years after coal fly ash (cfa) soil amendment, mte bioavailability and uptake by two plants, lolium perenne and trifolium repens, were evaluated, as some biological markers reflecting physiological stress. results showed that the two plant species under study were suitable to reduce the mobility and the availability of these elements. moreover, the plant growth was better on cfa amended mte-contaminated soils, and the plant sensitivity to mte-induced physiological stress, as studied through photosynthetic pigment contents and oxidative damage was lower or similar. in conclusion, these results supported the usefulness of aided phytostabilisation of mte-highly contaminated soils. highlights ► aided phytostabilisation aims to establish a vegetation cover in order to promote immobilisation of mte. ► 8 years after the soil amendments, a pot culture study was carried out in greenhouse conditions. ► mte bioavailability and uptake by the two plants was drastically decreased with amendments. ► our results support the usefulness of aided phytostabilisation of mte-highly contaminated soils. ► cfa addition contributed to the reduction of the mte mobility and availability for the plants},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Pelfrene2011,

title = {In vitro digestion and DGT techniques for estimating cadmium and lead bioavailability in contaminated soils: Influence of gastric juice pH},

author = {Aurélie Pelfrêne and Christophe Waterlot and Francis Douay},

year  = {2011},

date = {2011-01-01},

journal = {Science of the Total Environment},

volume = {409},

pages = {5076-5085},

abstract = {A sensitivity analysis was conducted on an in vitro gastrointestinal digestion test (i) to investigate the influence of a low variation of gastric juice ph on the bioaccessibility of cd and pb in smelter-contaminated soils (fb, using the unified bioaccessibility method ubm) and fractions of metals that may be transported across the intestinal epithelium (fa, using the diffusive gradient in thin film technique), and (ii) to provide a better understanding of the significance of ph in health risk assessment through ingestion of soil by children. the risk of metal exposure to children (hazard quotient, hq) was determined for conditions that represent a worst-case scenario (i.e., ingestion rate of 200 mg day−1) using three separate calculations of metal daily intake: estimated daily intake (edi), bioaccessible edi (edi-fb), and oral bioavailable edi (edi-fa). the increasing ph from 1.2 to 1.7 resulted in: (i) no significant variation in cd-fb in the gastric phase but a decrease in the gastrointestinal phase; (ii) a decrease in soluble pb in the gastric phase and a significant variation in pb-fb in the gastrointestinal phase; (iii) a significant decrease in cd-fa and no variation in pb-fa; (iv) no change in edi-fb and edi-fa hqs for cd; (v) a significant decrease in edi-fb hqs and no significant variation in edi-fa hq for pb. in the analytical conditions, these results show that risk to children decreases when the bioavailability of pb in soils is taken into account and that the studied ph values do not affect the edi-fa hqs. the present results provide evidence that the inclusion of bioavailability analysis during health risk assessment could provide a more realistic estimate of cd and pb exposure, and opens a wide field of practical research on this topic (e.g., in contaminated site management).},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Pelfrene2011a,

title = {Investigation of DGT as a metal speciation tool in artificial human gastrointestinal fluids},

author = {Aurélie Pelfrêne and Christophe Waterlot and Francis Douay},

year  = {2011},

date = {2011-01-01},

journal = {Analytica Chimica Acta},

volume = {699},

pages = {177-186},

abstract = {This paper reports the results of an investigation on the performance of the diffusive gradient in thin film technique (dgt) as a speciation tool for trace elements (tes) in artificial human gastrointestinal fluids. the validity of cd, pb, and zn sampling by dgt in digestive fluids was checked. the te bioaccessibility in highly contaminated soils was determined using the in vitro unified barge method (ubm) test. dgt devices were deployed in the gastrointestinal solutions obtained after carrying out the ubm test. the computer speciation code jess (joint expert speciation system) was used to predict the metal speciation of cd, pb, and zn. combining the in vitro test with the dgt technique and jess provided an approach to the te species available for transport across the intestinal epithelium. the gastrointestinal absorption of ingested te ranged from 8 to 30% for cd, 0.6 to 11% for pb, and 0.8 to 7% for zn and was influenced by te speciation. in this original approach, the dgt technique was found to be simple and reliable in the investigation of te chemical speciation in digestive fluids. extrapolation to the in vivo situation should be undertaken very cautiously and requires further investigation.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Pelfrene2011b,

title = {Assessing Cd, Pb, Zn mobility and human bioaccessibility in smelter-contaminated agricultural topsoils (northern France)},

author = {Aurélie Pelfrêne and Christophe Waterlot and Muriel Mazzuca and Catherine Nisse and Géraldine Bidar and Francis Douay},

year  = {2011},

date = {2011-01-01},

journal = {Environmental Geochemistry and Health},

volume = {33},

number = {5},

pages = {477-493},

abstract = {The extractability of cd, pb, and zn was investigated in contaminated agricultural topsoils located in an area highly affected by the past atmospheric emissions of two smelters in northern france in order to assess their mobility and human bioaccessibility. the determination of cd, pb, and zn bioaccessibility (unified barge method, in vitro test) was made to evaluate the absolute trace element (te) bioavailability. the results highlighted differences in bioaccessibility between cd, pb, and zn (cd[pb[ zn). the mean values of the bioaccessible fractions of cd, pb, and zn during the gastric phase were 82, 55, and 33%, respectively, of the pseudototal concentrations, whereas during the gastrointestinal phase, the bioaccessible fractions of metals decreased to 45, 20, and 10%, respectively. stepwise multiple regression analysis showed that human bioaccessibility was affected by various physicochemical parameters (i.e., sand, carbonates, organic matter, assimilated p, free al oxides, and pseudototal fe contents). sequential extractions were performed as an indication of the te availability in these soils. cadmium occurred in the more available fractions, pb was mostly present as bound by oxides, and a significant contribution to the pseudototal zn concentration was defined as the unavailable residual form related to the crystalline structures of minerals. the concepts of bioavailability and bioaccessibility are important for quantifying the risks associated with exposure to environmental pollutants and providing more realistic information for human health},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Pelfrene2011c,

title = {Assessing Cd and Pb human bioaccessibility in smelter-contaminated agricultural and urban soils (northern France)},

author = {Aurélie Pelfrêne and Christophe Waterlot and Catherine Nisse and Muriel Mazzuca and Francis Douay},

year  = {2011},

date = {2011-01-01},

booktitle = {11th International Conference on the Biogeochemistry of Trace Elements (ICOBTE). 3-7 July 2011, Florence (Italy)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Pourrut2011a,

title = {Assessment of fly ash-aided phytostabilisation of highly contaminated soils after an 8-year field trial. Part 2. Influence on plants},

author = {Bertrand Pourrut and Alena Lopareva-Pohu and Christelle Pruvot and Guillaume Garçon and Anthony Verdin and Christophe Waterlot and Géraldine Bidar and Pirouz Shirali and Francis Douay},

year  = {2011},

date = {2011-01-01},

journal = {Science of the Total Environment},

volume = {409},

pages = {4504-4510},

abstract = {Aided phytostabilisation is a cost-efficient technique to manage metal-contaminated areas, particularly in the presence of extensive pollution. plant establishment and survival in highly metal-contaminated soils are crucial for phytostabilisation success, as metal toxicity for plants is widely reported. a relevant phytostabilisation solution must limit metal transfer through the food chain. therefore, this study aimed at evaluating the long-term efficiency of aided phytostabilisation on former agricultural soils highly contaminated by cadmium, lead, and zinc. the influence of afforestation and fly ash amendments on reducing metal phytoavailability was investigated as were their effects on plant development. before being planted with a tree mix, the site was divided into three plots: a reference plot with no amendment, a plot amended with silico-aluminous fly ash and one with sulfo-calcic fly ash. unlike salix alba and quercus robur, alnus glutinosa, acer pseudoplatanus and robinia pseudoacacia grew well on the site and accumulated, overall, quite low concentrations of metals in their leaves and young twigs. this suggests that these three species have an excluder phenotype for cd, zn and pb. after 8 years, metal availability to a. glutinosa, a. pseudoplatanus and r. pseudoacacia, and translocation to their above-ground parts, strongly decreased in fly ash-amended soils. such decreases fit well together with the depletion of cacl2-extractable metals in amended soils. although both fly ashes were effective to decrease cd, pb and zn concentrations in above-ground parts of trees, the sulfo-calcic ash was more efficient.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2011,

title = {Analysis of cadmium in water extracts from contaminated soils with high arsenic and iron concentration levels},

author = {Christophe Waterlot and Géraldine Bidar and Christelle Pruvot and Francis Douay},

year  = {2011},

date = {2011-01-01},

journal = {Journal of Environmental Science and Engineering},

volume = {5},

pages = {271-280},

abstract = {Cadmium (cd) concentrations in the water extracts often contaminated soils by arsenic (as) with various iron (fe) contents were measured using inductively coupled plasma atomic emission spectrometry (icp-aes) and electrothermal atomic absorption spectrometry (etaas). due to the potential spectral interferences of as and fe in the quantification of cd, two methods were used for the background compensation. the first method was based on the use of a deuterium lamp (bgcd2 mode). the second one required a cd hollow cathode lamp pulsated with a current for which the intensity varies in the course of time (bgcsr mode). the results showed that the choices of the analytical technique and the method used for the background compensation depend on the cd, as and fe concentrations in the solution and the concentration ratios as/cd or/and fe/cd. in comparison with the icp-aes and the etaas in combination with the bgcd2 mode, it was shown that the high-speed self-reversal method (hssr) was a more appropriate method to correct as and fe spectral interferences during the cd measurements. on the other hand, depending on the cd concentration, it was established that no significant fe interference occurred during the cd determination even if fe concentration was 50 mg/l, reflecting the efficiency of the hssr method to overcome the fe interferences in the determination of cd concentration by etaas without any matrix modifier in water extracts},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2011a,

title = {DFT calculations on the Friedel-Crafts benzylation of 1,4-dimethoxybenzene using ZnCl2 impregnated montmorillonite K10 — inversion of relative selectivities and reactivities of aryl halides},

author = {Christophe Waterlot and Daniel Couturier and Benoit Rigo and Alina Ghinet and Marc De-Backer},

year  = {2011},

date = {2011-01-01},

journal = {Chemical Papers},

volume = {65},

number = {6},

pages = {873-882},

abstract = {Zinc was bound on montmorillonite k10 by cation exchange to obtain a catalyst named clayzic. in the friedel-crafts benzylation of 1,4-dimethoxybenzene, this catalyst was used for the synthesis of substituted diphenylmethanes using 4-chlorobenzyl chloride and 4-bromobenzyl bromide. during the reaction, sub-products from a second benzylation reaction process were observed. for a better understanding of their formation, reactions were carried out at different times to obtain data on the progress of benzylation and the relative ratio of each product was calculated using two different analytical methods. it was shown that the selectivity and reactivity of both aryl halides were reversed under these experimental conditions contrary to those obtained using the more conventional catalyst, zinc dichloride. these results were explained by geometrical and electronic considerations. it was found that the formation of transition states and wheland intermediates from aryl bromide and chloride in the presence of clayzic can be explained in terms of preferential absorption. moreover, the high percentage of 4-chlorobenzyl chloride conversion was attributed to its covalent radius, which is smaller than that of br. at the same time it was shown that the presence of a brönsted acid, due to the liberation of hcl during the benzylation, is responsible for the poisoning of the clayzic catalyst. moreover, poisoning effect of the bromine anion could not be excluded},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Waterlot2011d,

title = {Lead mobility and bioaccessibility in kitchen garden soils (northern France) after immobilization with a mixture of phosphates},

author = {Christophe Waterlot and Aurélie Pelfrêne and Francis Douay},

year  = {2011},

date = {2011-01-01},

booktitle = {11th International Conference on the Biogeochemistry of Trace Elements (ICOBTE). 3-7 July 2011, Florence (Italy)},

note = {ACTI},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Waterlot2011b,

title = {Effects of a phosphorus amendment and the pH of water used for watering on the mobility and phytoavailability of Cd, Pb and Zn in highly contaminated kitchen garden soils},

author = {Christophe Waterlot and Christelle Pruvot and Henri Ciesielski and Francis Douay},

year  = {2011},

date = {2011-01-01},

journal = {Ecological engineering},

volume = {37},

number = {7},

pages = {1081-1093},

abstract = {Studies on two lead and zinc smelters in northern france (metaleurop nord and umicore) showed that the level of metallic contamination of kitchen garden soils is higher than the agricultural soils located in the same environment. this results most particularly from cropping practices and the addition of various products. due to the physical and chemical parameters of these soils, the behaviour and transfer of pollutants towards various plants (grass, trees, and vegetables) may be perceptibly different than what is observed on agricultural soils. for a better understanding of pollutant behaviour in kitchen garden topsoils, the cd, pb and zn was fractionated using the sm&t protocol and various extracting solutions (cacl2, acetic acid, and citric acid) to evaluate their mobility in two highly contaminated soils chosen in the area affected by the past atmospheric emissions of the two smelters. in addition, agricultural topsoil was sampled in a non-massively contaminated area and was therefore chosen as the control soil. the three soils were amended with a mixture of hydroxyapatite (ha) and diammonium phosphate (dap). at 6 months, extracting procedures were carried out to evaluate the effects of the amendment on the mobility of cd, pb and zn. this step was then supplemented by an evaluation of the impact of the amendment on the phytoavailability of pollutants, which was determined in plant uptake studies with ryegrass (lolium perenne l.) by considering only the pollutant concentrations in their shoots. two experiments were carried out. in the first one, unamended and amended soils and ryegrass were watered with distilled water (ph = 7). in the second one, osmosed water (ph = 5.5) was used to evaluate the effects of the acid water–phosphate amendment system on the mobility and phytoavailability of cd, pb and zn. six months after the start of the experiments, the selective extractions showed that the effectiveness of the amendment studied depended on the element, the soil and the water's ph. reductions of metal eluted from the contaminated soils were 1.5–37.9% for cd, and 9.1–80.9% for pb. application of p amendment to the combination of osmosed water was generally the most effective for immobilising cd and pb elution. in contrast, the mixture of ha and dap was ineffective for reducing zn elution. the plant-fresh biomass yield was significantly (p < 0.05) increased by the combination of p amendment and distilled water, whereas a reduction of biomass was recorded with the combined amendment and osmosed water. addition of p amendment generally reduced pb uptake in ryegrass shoots (1–47%), while both cd and zn were increased by 17.9–79% and 0.45–100%, respectively.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2011c,

title = {Structure and physical properties in crosslinked polyurethanes},

author = {Véronique Waterlot and Daniel Couturier and Christophe Waterlot},

year  = {2011},

date = {2011-01-01},

journal = {Journal of Applied Polymer Science},

volume = {119},

number = {3},

pages = {1742-1751},

abstract = {Crosslinked polyurethanes based on a mixture of toluene diisocyanate, polypropylene glycol, trimethylol propane (tmp), glycerol (gly), and desmophen (des) were synthesized with various ratios of des and gly. chemical crosslinks were introduced through the hard segment (tmp, gly) and through the soft segment (des). the effects of the degree of crosslinking on the properties were examined. the crystallinity of the obtained polymers were studied by using modulated differential scanning calorimetry, differential scanning calorimetry, dynamic mechanical analysis and their morphology was studied by atomic force microscopy. it appeared that the degree of crosslinking increased according to the increase of gly content. moreover, it was found that chemical crosslinks in the hard segment destroyed the crystallinity of the hard phase and reduced the mobility of the soft phase, improving the heat stability of the hard domains, and modifying the mechanical properties of polyurethane films.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Fritsch2010,

title = {Spatial distribution of metals in smelter-impacted soils of woody habitats: Influence of landscape and soil properties, and risk for wildlife},

author = {Clémentine Fritsch and Patrick Giraudoux and Michaël Coeurdassier and Francis Douay and Francis Raoul and Christelle Pruvot and Christophe Waterlot and Annette De-Vaufleury and Renaud Scheifler},

year  = {2010},

date = {2010-01-01},

journal = {Chemosphere},

volume = {81},

pages = {141-155},

abstract = {The spatial distribution of total and cacl2-extracted cd, pb and zn concentrations in smelter-impacted soils was investigated over a polluted site (40 km2) in northern france. the study was conducted on 262 soils sampled in woody habitats. total and extracted concentrations of trace metals (tms) rose up to 2402 and 59.5 mg kg1 for cd, 41 960 and 13.7 mg kg1 for pb, 38 760 and 143.0 mg kg1 for zn, respectively. the spatial dependence of both total and extracted concentrations showed a high spatial auto-correlation and ordinary kriging was used to predict soil concentrations. investigating which variables influenced metal concentrations and their spatial distribution, we found that total concentrations mostly depended on the distance from the smelter, the wind and the organic carbon, while extracted concentrations were mainly modulated by the ph. conditionally to those variables, other soil properties and landscape characteristics influenced both total and extracted concentrations. we conclude that total and extracted tm concentrations are governed by different processes which act at various spatial ranges: total concentrations are mainly related to input and retention of metals (large scale) whereas extracted concentrations were mainly explained by factors controlling metal solubility in soils (local scale). spatial distributions of total and extracted tms differed over the area, which should be considered for risk assessment. maps of risk based on the us epa’s eco-ssls (for plants, invertebrates and vertebrates) were realized, showing that wildlife may be at risk but that the relevance of eco-ssl values is questionable.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Pohu-Lopareva2010,

title = {Asymétrie fluctuante des feuilles d’Alnus glutinosa: Indicateur de stress dans le cadre d’une phytostabilisation aidée sur des sols fortement pollués par des éléments traces métalliques},

author = {Alena Pohu-Lopareva and Hélène Roussel and Christophe Waterlot and Guillaume Garçon and Géraldine Bidar and Christelle Pruvot and Denis Baize and A Shirali and Francis Douay},

year  = {2010},

date = {2010-01-01},

journal = {Bulletin Bodenkundliche Gesellschaft der Schweiz},

volume = {30},

pages = {51-56},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Roussel2010,

title = {Cd, Pb and Zn oral bioaccessibility of urban soils contaminated in the past by atmospheric emissions from two lead and zinc smelters},

author = {Hélène Roussel and Christophe Waterlot and Aurélie Pelfrêne and Christelle Pruvot and Muriel Mazzuca and Francis Douay},

year  = {2010},

date = {2010-01-01},

journal = {Archives of Environmental Contamination and Toxicology},

volume = {58},

number = {4},

pages = {945-954},

abstract = {Ingestion of dust or soil particles could pose a potential health risk due to long-term metal trace element (mte) exposure. twenty-seven urban topsoil samples (kitchen garden and lawn) were collected and analyzed for cd, pb and zn using the unified bioaccessibility research group of europe (barge) method (ubm) test to estimate the human bioaccessibility of these elements. the quantities of cd, pb and zn extracted from soils indicated, on average, 68, 62 and 47% bioaccessibility, respectively, in the gastric phase and 31, 32 and 23% bioaccessibility, respectively, in the gastro-intestinal phase. significant positive correlations were observed between concentrations extracted with ubm and total mte contents. stepwise multiple regression analysis showed that human bioaccessibility was also affected by some physico-chemical soil parameters (i.e. total nitrogen, carbonates, clay contents and ph). the unified test presents some valuable data for risk assessment. indeed, the incorporation of oral bioaccessible concentrations into risk estimations could give more realistic information for health risk assessment.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2010,

title = {Reduction of dissolved oxygen in boiler water using new redox polymers},

author = {Christophe Waterlot and Daniel Couturier},

year  = {2010},

date = {2010-01-01},

journal = {Journal of Applied Polymer Science},

volume = {118},

pages = {7-16},

abstract = {New polymers were used as catalysts for the removal of dissolved oxygen in boiler water. these polymers, based upon hydroquinone-quinone redox system, were prepared by polymerization of methyl 4-(2,5- dimethoxybenzyl)cinnamate and copolymerization of this monomer with 4-(40-vinylphenethyl)-1,10-phenanthroline. the resulting product was used to synthesize polymers containing transition metal ions. nuclear magnetic resonance, infrared spectroscopy, and elemental analysis were achieved to characterize monomers and/or electron- transfer polymers. these polymers were used for the removal of oxygen from water. it was shown that the oxygen content was reduced to less than 0.1 mg l in 70 s. based on the obtained results, the redox capacity of two polymers were determined. it was established that the poly-4-(2,5-dihydroxybenzyl)cinnamic acid reached a redox capacity of 69.7 mg of o2 per gram of polymer.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2010a,

title = {Evaluation des effets d’une lampe à cathode creuse pulsée à courant variable sur les interférences spectrales de l’arsenic dans le dosage du cadmium par spectrophotométrie d’absorption atomique},

author = {Christophe Waterlot and Francis Douay},

year  = {2010},

date = {2010-01-01},

journal = {Revue Canadienne de Génie Civil},

volume = {37},

pages = {346-353},

abstract = {Les teneurs en cadmium de solutions aqueuses contaminees a des teneurs croissantes en arsenic ont ete mesurees a l’aide d’un spectrophotome`tre d’absorption atomique equipe d’une flamme et d’un four electrothermique. pour chacune des techniques d’atomisation, a ete evaluee une me´thode de correction du bruit de fond base´e sur le renversement de spectre et connue sous le nom de « high-speed self-reversal method », au regard d’une autre methode plus couramment utilisee ne ne´cessitant qu’une lampe au deute´rium et une lampe au cadmium. en comparaison avec cette me´thode, les re- sultats ont montre que le mode de correction base´ sur le renversement spectral via l’utilisation d’une lampe au cadmium a` cathode creuse pulse´e a` courant variable etait une methode plus approprie´e pour corriger les phe´nome`nes d’interfe´rences causes par l’arsenic (as) lors du dosage du cadmium (cd). il a ete etabli que cette methode permettait de s’affranchir des interferences de l’arsenic pour des ratios [as]/[cd] infe´rieurs a` 50 000.},

note = {ACLN},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Douay2009,

title = {Contamination of woody habitat soils around a former lead smelter in the North of France},

author = {Francis Douay and Christelle Pruvot and Christophe Waterlot and Clémentine Fritsch and Hervé Fourrier and Alexandre Loriette and Géraldine Bidar and Cécile Grand and Annette De-Vaufleury and Renaud Scheifler},

year  = {2009},

date = {2009-01-01},

journal = {Science of the Total Environment},

volume = {407},

number = {21},

pages = {5564-5577},

abstract = {The Contamination of the Topsoil of 262 Woody Habitats around a Former Lead Smelter in the North of France Was Assessed. in This Urbanized and Industrialized Area, These Kinds of Habitats Comprise of Hedges, Groves, Small Woods, Anthropogenic Creations and One Large Forest. except for the Latter, Which Is 3 Km Away, These Woody Habitat Soils Often Present a High Anthropization Degree (a Significant Amount of Pebbles and Stones Related to Human Activities) with a High Metal Contamination. in the Studied Woody Habitat Topsoils, Cd, Pb and Zn Concentrations Largely Exceeded Those of Agricultural Topsoils Located in the Same Environmental Context. Therefore, Atmospheric Emissions from the Smelter Are Not the Only Cause of the High Contamination of the Woody Habitat Soils. This Last One Is Related to the Nature and the Contamination Level of Deposit in Relation with Human Activities (rubbles, Slag, Soils, Etc). with Regard to the Results Obtained with Chemical Extractions, the Mobility of Cd, Pb and Zn in These Soils Is Also Greater Than in Agricultural Soils. in the Forest, Pollutant Solubility Is Increased by Soil Acidic Ph. the Variability of the Physico-chemical Parameters and the High Metal Contamination of the Topsoils Are the Main Characteristics of the Woody Habitats Located around the Former Smelter. Although Never Taken into Account during Risk Assessment, the Disturbance of These Environmental Components Could Have Important Biogeochemical Impacts (nutrients and Metal Cycles). Moreover, Any Modification of the Soils' Use Could Potentially Cause Mobilization and Transfer of the Pollutants to the Biosphere. Six Years After the Closure of the Smelter, and As Social and Economic Pressures Considerably Increase in This Area, the Study of These Peculiar Ecosystems Is Necessary to Understand and Predict the Bioavailability, Transfer, Bioaccumulation and Effects of Pollutants in Food Chains.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Waterlot2009,

title = {The problem of arsenic interference in the analysis of Cd to evaluate its extractability in soils contaminated by arsenic},

author = {Christophe Waterlot and Francis Douay},

year  = {2009},

date = {2009-01-01},

journal = {Talanta},

volume = {80},

pages = {716-722},

abstract = {The arsenic (as) spectral interference observed in the determination of cadmium (cd) by inductively coupled plasma atomic emission spectrometry (icp-aes) was studied in atomic absorption spectrometry (aas) using flame (faas) and graphite furnace (gfaas) as atomizers. the soils of 15 kitchen gardens located near two smelters in the north of france were selected according the ratio as/cd. four different extracting solutions usually used to evaluate the mobility of cd were chosen to extract cd from these soil samples: citric acid 0.11 m, acetic acid 0.11 m, calcium chloride 0.01m and water. the quantitative determinations of cd in the 15 soils for each solvent were investigated by icp-aes at two lines (228.802 and 214.438 nm) and by faas or gfaas with two-way background compensation. compared to the cd concentrations measured in the acid solutions and in the cacl2 solution after the addition of a chemical modifier, it was clearly demonstrated that the high-speed self-reversal background compensation (hssr-method) was the method of choice to eliminate the spectral interference of as during cd determination by faas and by gfaas. in water, it was shown that the deuterium lamp used for the background compensation (d2-method) was able to eliminate the most of the as interference. in comparison with cd concentrations in water after adding a chemical modifier, those obtained with the hssr-method were similar and a very good correlation was obtained between these two methods (r2 = 0.995). it was therefore established that the hssr-method would be able to replace the chemical modifiers to eliminate as interference in the determination of cd-extractable from as contaminated soils.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@conference{Waterlot2009a,

title = {Évaluation d'une méthode de dosage du cadmium dans les solutions d’extraction de sols de potagers localisés aux alentours d’une ancienne fonderie de plomb},

author = {Christophe Waterlot and Christelle Pruvot and Francis Douay},

year  = {2009},

date = {2009-01-01},

booktitle = {2ème Rencontres Nationales de la Recherche sur les Sites & Sols Pollués, ADEME. 20-21 octobre 2009, Paris},

abstract = {Les activités passées de la fonderie de plomb metaleurop nord (1873 – 2003) située à noyelles- godault ont entraîné des rejets dans l’atmosphère de quantités importantes de poussières. ceux-ci ont engendré une forte contamination des sols aux alentours. des travaux antérieurs ont ainsi montré que les concentrations totales en cd, pb et zn dans les horizons labourés des sols agricoles du secteur excédaient fortement les teneurs agricoles habituelles régionales (sterckeman et al. 2000). dans une moindre mesure, il a aussi été constaté une contamination en ag, as, bi, cu, hg, in, ni, sb, se, sn et tl (sterckeman et al. 2002). plus récemment, douay et al. (2008) ont mis en évidence un degré de contamination en cd, pb et zn, mais aussi en bi, in, sb et as des sols urbains du secteur, supérieur à celui des sols agricoles situés dans le même contexte environnemental. ceci pourrait être expliqué par des remaniements et les pratiques des jardiniers, mais aussi par des apports en liaison avec l’utilisation du charbon pour les besoins domestiques. quels que soient leurs usages, les sols aux alentours de metaleurop nord présentent une contamination multi métallique pouvant engendrer des interférences lors du dosage de certains éléments. ainsi, les teneurs en cd mesurées peuvent différer selon les techniques analytiques utilisées. la détermination des teneurs en cd dans les solutions d’extraction en utilisant la spectrométrie d’émission atomique couplée à une torche à plasma (icp-aes : inductively coupled plasma – atomic emission spectrometry) peut présenter des inconvénients. ceux-ci sont particulièrement rencontrés lorsque les matrices digérées présentent des teneurs élevées en fe, en al, mais aussi en as, en comparaison avec celles en cd (lambkin and alloway 2000). c’est notamment le cas des sols aux alentours de metaleurop nord. par ailleurs, les longueurs d’onde 228,802 nm, 214,438 nm et 226,502 nm caractéristiques des raies d’émission de cd définissent celles relatives aux émissions secondaires respectivement de as, al et fe. des interférences spectrales causées par ces éléments au cours du dosage de cd peuvent donc engendrer une surévaluation ou une sous-évaluation des teneurs en cd mesurées (lambkin and alloway 2000). le dosage de cd par spectrométrie d’absorption atomique à la longueur d’onde caractéristique de la raie principale d’absorption de cet élément permet de s’affranchir des interférences spectrales causées par fe et al. cette technique de dosage élimine partiellement les interférences spectrales entre as et cd (lambkin and alloway 2000). en revanche, les caractéristiques des spectromètres d’absorption atomique (saa) ne permettent pas à ce jour de s’affranchir totalement des interférences spectrales entre ces deux éléments, et ceci en raison des longueurs d’onde d’absorption très proches qui les caractérisent (ıcd = 228,802 nm et ıas = 228,812 nm). la présentation porte sur l’évaluation d’une méthode de correction du bruit de fond généré par as au cours du dosage de cd dans les solutions d’extraction de sols urbains présentant des teneurs en as parfois supérieures à celles en cd. localisés à moins de 2 km de l’ancien site métallurgique metaleurop nord, les horizons labourés de 10 sols de potagers ont été échantillonnés. après séchage à une température inférieures à à 40°c, les terres ont été homogénéisées, puis broyées pour passer au travers d’un tamis de 315 μm. des extractions à l’eau ultra pure, au chlorure de calcium 0,01 m et à l’acide citrique 0,11 m ont été réalisées en triplicat sur chacun des échantillons composites. les teneurs en cd et en as des solutions obtenues ont été déterminées par icp-aes aux longueurs d’onde ıcd = 228,802 nm, ııcd = 214,438 nm et ıas = 193,696 nm. la démarche a été complétée par la détermination en saa de leurs teneurs en cd en utilisant deux modes de correction du bruit causée par l’espèce concomitante, as. le premier, appelé bgc-d2 (deuterium background correction) nécessite l’utilisation d’une lampe au deutérium. le second mode, dénommé bgc-sr (self reversal background correction), est basé sur l’utilisation d’une lampe cd à cathode creuse pulsée avec un courant dont l’intensité varie au cours du temps.},

note = {ACTN},

keywords = {ER4},

pubstate = {published},

tppubtype = {conference}

}

@article{Douay2008,

title = {Contamination of urban soils in an area of Northern France polluted by dust emissions of two smelters},

author = {Francis Douay and Christelle Pruvot and Hélène Roussel and Henri Ciesielski and Hervé Fourrier and Nicolas Proix and Christophe Waterlot},

year  = {2008},

date = {2008-01-01},

journal = {Water Air and Soil Pollution},

volume = {188},

number = {1-4},

pages = {247-260},

abstract = {The contamination of 27 urban topsoils has been assessed around two lead and zinc smelters (metaleurop nord and umicore) in the north of france. eighteen trace elements have been analysed (ag, as, bi, cd, co, cr, cu, hg, in, ni, pb, sb, se, sn, tl, th, u and zn). the investigation included the study of the vertical distribution of cd, pb and zn as indicators of pollution. it was shown that cd, in, pb, sb and zn were major pollutants followed in lesser quantities by ag, bi, cu and hg. in addition, as, ni, se, sn and tl were present at levels slightly higher than regional agricultural values. the other elements (co, cr, th and u) were at endogenous levels. the observations have highlighted the strong heterogeneity of the physico-chemical parameters of urban soils and the existence of heavy contamination of the under layers by cd, pb and zn. a potential transfer of metals from the topsoil to the deeper layers and especially cd and zn, is not excluded. indeed the soil rework is not the only factor explaining contamination level of the deeper layers of the studied soils. the comparison of the studied element concentrations in urban soils with nearby local agricultural values shows that the dust emission originating from the metaleurop and umicore smelters were not the only source of contamination. thus a large contamination of the studied urban soils by sb and in could be explained by domestic combustion of coal for heating.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}

@article{Douay2008b,

title = {Impact of a smelter closedown on metal contents of wheat cultivated in the neighbourhood},

author = {Francis Douay and Hélène Roussel and Christelle Pruvot and Christophe Waterlot},

year  = {2008},

date = {2008-01-01},

journal = {Environmental Science and Pollution Research},

volume = {15},

pages = {162-169},

abstract = {Background. the contamination of soils by heavy metals engenders important environmental and sanitary problems in northern france where a smelter has been located for more than one hundred of years. it has been one of the most important pb production sites in europe until its closedown in march 2003. ore smelting process generated considerable atmospheric emissions of dust. despite an active environmental strategy, these emissions were still significant in 2002 with up to 17 tonnes of pb, 32 tonnes of zn and 1 tonne of cd. over the years, the generated deposits have led to an important contamination of the surrounding soils. previous studies have shown pollutant transfers to plants, which can induce a risk for human and animal health. the objective of this study was to evaluate the consequences of the smelter closedown on the cd and pb contents of wheat (grain and straw) cultivated in the area. methods. paired topsoil and vegetable samples were taken at harvest time at various distances to the smelter. the sample sites were chosen in order to represent a large range of soil metal contamination. sampling was realised on several wheat harvests between 1997 and 2003. 25 samples were collected before the smelter closedown and 15 after. all ears of about 1 m long of two rows were manually picked and threshed in the lab. similarly, straw was harvested at the same time. total metal contents in soil and wheat samples were quantified. results. a negative correlation between metal concentrations in soil and the distance to the smelter was shown. the wheat grain and straw showed significant cd and pb contents. the straw had higher metal contents than the grain. during the smelter activity, the grain contents were up to 0.8 mg kg–1 dm of cd and 8 mg kg–1 dm of pb. for the straw, maximum contents were 5 mg kg–1 dm of cd and 114 mg kg–1 dm of pb. after the smelter closedown, we observed a very large decrease of pb in the grain (82%) and in the straw (91%). a smaller decrease was observed for cd in grain. despite this improvement, 80% of the studied samples remained non-acceptable for human consumption, according to the european legislation values, due to a high cd content. discussion. results highlighted a difference in metal accumulation in the plant organs as well as a difference in metal uptake. the approach pointed out the importance of atmospheric fallout in the wheat contamination pathways for pb. the smelter closedown has lead to a decrease of the pb content in wheat. it is interesting to relate this finding with the lead blood levels in children living close to the smelter. conclusions. those results have confirmed the importance of dust fallout in the plant contamination pathways. before the closedown, pb measured in the plant was principally originating from the smelter dust emissions. it raised the question of the sanitary risks for humans and animals living in the surrounding area of the smelter. recommendations and perspectives. in the literature, very few articles take the dust deposit as contamination pathways for crops into consideration. however, in highly contaminated sites, this pathway can be very important. thus, it would be worthy studying the uptake of metal contaminants by plants through the foliar system.},

note = {ACL},

keywords = {ER4},

pubstate = {published},

tppubtype = {article}

}